LOCUS       pSC-pGAL10-CRISPRa     15201 bp ds-DNA     circular     19-MAY-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-62283-sequence-102117.gbk
FEATURES             Location/Qualifiers
     misc_feature    38..683
                     /label="Homology"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        276..683
                     /label="LEU2 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /gene="S. cerevisiae LEU2"
     promoter        2509..3173
                     /label="GAL1,10 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="divergent inducible promoter, regulated by Gal4"
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
     primer_bind     complement(2522..2545)
                     /label="GAL1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    2840..2957
                     /label="UAS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="upstream activating sequence mediating Gal4-dependent induction"
                     /bound_moiety="Gal4"
     CDS             3185..5164
                     /label="MCP-VPR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSLEMPKKKRKVGSMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYGSGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLINSRSSGSPKKKRKVGSQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLGSGSGSRDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF*"
     CDS             3200..3220
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     CDS             3593..3742
                     /label="VP64"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDML"
     CDS             3767..3787
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     CDS             4217..4573
                     /label="RelA (p65) AD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="transcriptional activation domain of human RelA, also known as p65 (O'Shea and Perkins, 2008)"
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALL"
     CDS             4592..5161
                     /label="Rta AD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="transcriptional activation domain from the human herpesvirus 4 (Epstein-Barr virus) replication and transcription activator Rta/BRLF1 (Hardwick et al., 1992; Chavez et al., 2015)"
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        5580..6244
                     /label="GAL1,10 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="divergent inducible promoter, regulated by Gal4"
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
     primer_bind     complement(5593..5616)
                     /label="GAL1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    5911..6028
                     /label="UAS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="upstream activating sequence mediating Gal4-dependent induction"
                     /bound_moiety="Gal4"
     CDS             6256..6264
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MLE"
     CDS             6265..10365
                     /label="dCas9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain"
                     /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             10378..10398
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     CDS             10402..10422
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     CDS             10426..10446
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    11179..11703
                     /label="Homology"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      11931..12386
                     /label="f1 ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
     promoter        13024..13128
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /gene="bla"
     CDS             13129..13989
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     complement(13347..13366)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    14084..14766
                     /label="ColE1 ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccgt tttttctcct
     2521 tgacgttaaa gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa
     2581 taagaagtaa tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt
     2641 gcatttatat atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa
     2701 ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt
     2761 gaaggtttgt ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta
     2821 ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg
     2881 cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct
     2941 cggcggcttc taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt
     3001 tccaaagaga aggttttttt aggctaagat aatggggctc tttacatttc cacaacatat
     3061 aagtaagatt agatatggat atgtatatgg atatgtatat ggtggtaatg ccatgtaata
     3121 tgattattaa acttctttgc gtccatccaa aaaaaaagta agaatttttg aaaattcaat
     3181 ataaatgtct ctcgagatgc ccaaaaagaa aagaaaagtg ggtagtatgg cttctaactt
     3241 tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg ccccaagcaa
     3301 cttcgctaac gggatcgctg aatggatcag ctctaactcg cgttcacagg cttacaaagt
     3361 aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt
     3421 gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta accattccaa ttttcgccac
     3481 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
     3541 gattccctca gcaatcgcag caaactccgg catctacggc tccggacgag ccgatgctct
     3601 cgacgatttt gacctcgata tgctgggatc cgacgctctc gatgacttcg accttgacat
     3661 gctggggagt gacgctctgg acgacttcga tctcgacatg ctgggctccg acgccctgga
     3721 tgacttcgat ctggacatgc tcatcaactc tagaagttcc ggatctccga aaaagaaacg
     3781 caaagttggt agccagtacc tgcccgacac cgacgaccgg caccggatcg aggaaaagcg
     3841 gaagcggacc tacgagacat tcaagagcat catgaagaag tcccccttca gcggccccac
     3901 cgaccctaga cctccaccta gaagaatcgc cgtgcccagc agatccagcg ccagcgtgcc
     3961 aaaacctgcc ccccagcctt accccttcac cagcagcctg agcaccatca actacgacga
     4021 gttccctacc atggtgttcc ccagcggcca gatctctcag gcctctgctc tggctccagc
     4081 ccctcctcag gtgctgcctc aggctcctgc tcctgcacca gctccagcca tggtgtctgc
     4141 actggctcag gcaccagcac ccgtgcctgt gctggctcct ggacctccac aggctgtggc
     4201 tccaccagcc cctaaaccta cacaggccgg cgagggcaca ctgtctgaag ctctgctgca
     4261 gctgcagttc gacgacgagg atctgggagc cctgctggga aacagcaccg atcctgccgt
     4321 gttcaccgac ctggccagcg tggacaacag cgagttccag cagctgctga accagggcat
     4381 ccctgtggcc cctcacacca ccgagcccat gctgatggaa taccccgagg ccatcacccg
     4441 gctcgtgaca ggcgctcaga ggcctcctga tccagctcct gcccctctgg gagcaccagg
     4501 cctgcctaat ggactgctgt ctggcgacga ggacttcagc tctatcgccg acatggactt
     4561 ctccgcactg ctgggtagcg gatcgggatc tcgggattcc agggaaggga tgtttttgcc
     4621 gaagcctgag gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc
     4681 aaaacgaatc cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc
     4741 cagcctcgca ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc
     4801 accagtccct cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt
     4861 ggaggatccc gatgaagaga cgagccaggc tgtcaaagcc cttcgggaga tggccgatac
     4921 tgtgattccc cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc
     4981 cccaaggggc catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa
     5041 cctggactca cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga
     5101 gtgcctcttg catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt
     5161 ttgaagtgct tttaactaag aattattagt cttttctgct tattttttca tcatagttta
     5221 gaacacttta tattaacgaa tagtttatga atctatttag gtttaaaaat tgatacagtt
     5281 ttataagtta ctttttcaaa gactcgtgct gtctattgca taatgcactg gaaggggaaa
     5341 aaaaaggtgc acacgcgtgg ctttttcttg aatttgcagt ttgaaaaata actacatgga
     5401 tgataagaaa acatggagta cagtcacttt gagaaccttc aatcagctgg taacgtcttc
     5461 gttaattgga tactcaaaaa agatggatag catgaatcac aagatggaag gaaatgcggg
     5521 ccacgaccac agtgatatgc atatgggaga tggagatgat acctggcccc ggtgctatag
     5581 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta
     5641 ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt
     5701 atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa
     5761 ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga
     5821 ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat
     5881 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
     5941 tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc
     6001 tgtcacccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca gttaagcgta
     6061 ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct ctttacattt
     6121 ccacaacata taagtaagat tagatatgga tatgtatatg gatatgtata tggtggtaat
     6181 gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt aagaattttt
     6241 gaaaattcaa tataaatgct cgaggacaag aagtattcta tcggactggc catcgggact
     6301 aatagcgtcg ggtgggccgt gatcactgac gagtacaagg tgccctctaa gaagttcaag
     6361 gtgctcggga acaccgaccg gcattccatc aagaaaaatc tgatcggagc tctcctcttt
     6421 gattcagggg agaccgctga agcaacccgc ctcaagcgga ctgctagacg gcggtacacc
     6481 aggaggaaga accggatttg ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc
     6541 gacgacagct tcttccatag gctggaagaa tcattcctcg tggaagagga taagaagcat
     6601 gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg cctatcacga gaaataccca
     6661 accatctacc atcttcgcaa aaagctggtg gactcaaccg acaaggcaga cctccggctt
     6721 atctacctgg ccctggccca catgatcaag ttcagaggcc acttcctgat cgagggcgac
     6781 ctcaatcctg acaatagcga tgtggataaa ctgttcatcc agctggtgca gacttacaac
     6841 cagctctttg aagagaaccc catcaatgca agcggagtcg atgccaaggc cattctgtca
     6901 gcccggctgt caaagagccg cagacttgag aatcttatcg ctcagctgcc gggtgaaaag
     6961 aaaaatggac tgttcgggaa cctgattgct ctttcacttg ggctgactcc caatttcaag
     7021 tctaatttcg acctggcaga ggatgccaag ctgcaactgt ccaaggacac ctatgatgac
     7081 gatctcgaca acctcctggc ccagatcggt gaccaatacg ccgacctttt ccttgctgct
     7141 aagaatcttt ctgacgccat cctgctgtct gacattctcc gcgtgaacac tgaaatcacc
     7201 aaggcccctc tttcagcttc aatgattaag cggtatgatg agcaccacca ggacctgacc
     7261 ctgcttaagg cactcgtccg gcagcagctt ccggagaagt acaaggaaat cttctttgac
     7321 cagtcaaaga atggatacgc cggctacatc gacggaggtg cctcccaaga ggaattttat
     7381 aagtttatca aacctatcct tgagaagatg gacggcaccg aagagctcct cgtgaaactg
     7441 aatcgggagg atctgctgcg gaagcagcgc actttcgaca atgggagcat tccccaccag
     7501 atccatcttg gggagcttca cgccatcctt cggcgccaag aggacttcta cccctttctt
     7561 aaggacaaca gggagaagat tgagaaaatt ctcactttcc gcatccccta ctacgtggga
     7621 cccctcgcca gaggaaatag ccggtttgct tggatgacca gaaagtcaga agaaactatc
     7681 actccctgga acttcgaaga ggtggtggac aagggagcca gcgctcagtc attcatcgaa
     7741 cggatgacta acttcgataa gaacctcccc aatgagaagg tcctgccgaa acattccctg
     7801 ctctacgagt actttaccgt gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg
     7861 atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg caattgtgga ccttctgttc
     7921 aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa
     7981 tgcttcgact ctgtggagat tagcggggtc gaagatcggt tcaacgcaag cctgggtacc
     8041 taccatgatc tgcttaagat catcaaggac aaggattttc tggacaatga ggagaacgag
     8101 gacatccttg aggacattgt cctgactctc actctgttcg aggaccggga aatgatcgag
     8161 gagaggctta agacctacgc ccatctgttc gacgataaag tgatgaagca acttaaacgg
     8221 agaagatata ccggatgggg acgccttagc cgcaaactca tcaacggaat ccgggacaaa
     8281 cagagcggaa agaccattct tgatttcctt aagagcgacg gattcgctaa tcgcaacttc
     8341 atgcaactta tccatgatga ttccctgacc tttaaggagg acatccagaa ggcccaagtg
     8401 tctggacaag gtgactcact gcacgagcat atcgcaaatc tggctggttc acccgctatt
     8461 aagaagggta ttctccagac cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc
     8521 cataaaccag agaacattgt catcgagatg gccagggaaa accagactac ccagaaggga
     8581 cagaagaaca gcagggagcg gatgaaaaga attgaggaag ggattaagga gctcgggtca
     8641 cagatcctta aagagcaccc ggtggaaaac acccagcttc agaatgagaa gctctatctg
     8701 tactaccttc aaaatggacg cgatatgtat gtggaccaag agcttgatat caacaggctc
     8761 tcagactacg acgtggacgc catcgtccct cagagcttcc tcaaagacga ctcaattgac
     8821 aataaggtgc tgactcgctc agacaagaac cggggaaagt cagataacgt gccctcagag
     8881 gaagtcgtga aaaagatgaa gaactattgg cgccagcttc tgaacgcaaa gctgatcact
     8941 cagcggaagt tcgacaatct cactaaggct gagaggggcg gactgagcga actggacaaa
     9001 gcaggattca ttaaacggca acttgtggag actcggcaga ttactaaaca tgtcgcccaa
     9061 atccttgact cacgcatgaa taccaagtac gacgaaaacg acaaacttat ccgcgaggtg
     9121 aaggtgatta ccctgaagtc caagctggtc agcgatttca gaaaggactt tcaattctac
     9181 aaagtgcggg agatcaataa ctatcatcat gctcatgacg catatctgaa tgccgtggtg
     9241 ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg agttcgtgta cggagactac
     9301 aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc aggagatcgg aaaggccacc
     9361 gcaaagtact tcttctacag caacatcatg aatttcttca agaccgaaat cacccttgca
     9421 aacggtgaga tccggaagag gccgctcatc gagactaatg gggagactgg cgaaatcgtg
     9481 tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac
     9541 atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta aggaatcaat cctccccaag
     9601 cgcaactccg acaagctcat tgcaaggaag aaggattggg accctaagaa gtacggcgga
     9661 ttcgattcac caactgtggc ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag
     9721 tctaagaagc tcaagagcgt gaaggaactg ctgggtatca ccattatgga gcgcagctcc
     9781 ttcgagaaga acccaattga ctttctcgaa gccaaaggtt acaaggaagt caagaaggac
     9841 cttatcatca agctcccaaa gtatagcctg ttcgaactgg agaatgggcg gaagcggatg
     9901 ctcgcctccg ctggcgaact tcagaagggt aatgagctgg ctctcccctc caagtacgtg
     9961 aatttcctct accttgcaag ccattacgag aagctgaagg ggagccccga ggacaacgag
    10021 caaaagcaac tgtttgtgga gcagcataag cattatctgg acgagatcat tgagcagatt
    10081 tccgagtttt ctaaacgcgt cattctcgct gatgccaacc tcgataaagt ccttagcgca
    10141 tacaataagc acagagacaa accaattcgg gagcaggctg agaatatcat ccacctgttc
    10201 accctcacca atcttggtgc ccctgccgca ttcaagtact tcgacaccac catcgaccgg
    10261 aaacgctata cctccaccaa agaagtgctg gacgccaccc tcatccacca gagcatcacc
    10321 ggactttacg aaactcggat tgacctctca cagctcggag gggatgaggg agctgatcca
    10381 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga
    10441 aaggtataga attctaccgg cggccgccac cgcggtggag ctctaagcaa atagctaaat
    10501 tatatacgaa ttaatattat gattaagtgt ttacgtgagt gcgatatttt tattactatc
    10561 ttatacagtt gtatatactc tataaaatga gttgtctatt aattaacgcg atgaatgctt
    10621 tctgggttta cctctccaac aactctagtt tacttctcaa tacattcaat tgtatttgat
    10681 ttgtcaatac ttcatcatta atcaattcta tagttttgtt tttctcgttt atttccaaat
    10741 ttaatgcatc aattttatta ttcaatttgt cgttgatttt ggttaatgat tttatggttt
    10801 gatctctggc attgattgtt tgtgttagtt tttcattatt gataattaaa ttatttaagt
    10861 tagttatcaa ctcggtgttt tcaagtttca agttttcaat ttctttagag tttattagat
    10921 ttgtcaaagt ttctgaattg cttgattggt cctgtagaag agtatttgtt gttgtggata
    10981 attgattcaa tttttgagac aattgctgga aggcgttgaa atatctagca tcaatctcat
    11041 ggtttttttc ccgagagtct cgtagattca attgttttaa tatatcttgg gaccactctt
    11101 gatttgaact catggaaatt aaactgggtg ttgtgttgtg gtgtaatgat tgtaccccct
    11161 ttgcttataa ttgtgtggcc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
    11221 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
    11281 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
    11341 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
    11401 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
    11461 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
    11521 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
    11581 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
    11641 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
    11701 gtaggccggc catttaaatg tttaaacggc cacgctggcc caattcgccc tatagtgagt
    11761 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    11821 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    11881 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
    11941 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    12001 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    12061 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    12121 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    12181 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    12241 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    12301 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
    12361 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
    12421 ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc taatttgtga
    12481 gtttagtatt aggatctcta cccttggcga aaagtcctct gccaacaatg atgatatctg
    12541 atccaccact tacaacttcg tcgacggttc tgtactgctg acccaatatg cgtatatata
    12601 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
    12661 aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa
    12721 ggtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    12781 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    12841 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    12901 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    12961 aataatggtt tcttagacgt cggcgcgccg aggacgtcag gtggcacttt tcggggaaat
    13021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    13081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    13141 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    13201 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    13261 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    13321 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    13381 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    13441 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    13501 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    13561 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    13621 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    13681 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    13741 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    13801 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    13861 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    13921 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    13981 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    14041 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    14101 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    14161 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    14221 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    14281 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    14341 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    14401 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    14461 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    14521 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    14581 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    14641 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    14701 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    14761 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    14821 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    14881 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    14941 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    15001 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    15061 gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat tgtgagcgga
    15121 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg aattaaccct
    15181 cactaaaggg aacaaaagct g
//