LOCUS       pHS-dCas9               8276 bp ds-DNA     circular     19-MAY-2025
DEFINITION  7930 bp ds-DNA    circular SYN 20-JAN-2014 .
COMMENT     Imported using the Genbank importer. File name:
            84239-attachment_rRoGDegu4mCD.gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label="M13F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="M13 fwd common sequencing primer, one of multiplesi milar variants"
                     /gene="M13F"
                     /note="common sequencing primer, one of multiple similarva riants"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        71..392
                     /label="5' PB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER 5' PB repeat"
                     /gene="5' PB"
                     /note="5' PB repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    422..947
                     /label="PGK Promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             960..5189
                     /label="dCas9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYASLGSGSPKKKRKVEDPKKKRKVDGIGSGSNG*"
     misc_feature    963..5063
                     /label="Sp dCas9"
                     /ApEinfo_revcolor="#5e06c0"
                     /ApEinfo_fwdcolor="#5e06c0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5067..5093
                     /label="HA tag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5112..5132
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5139..5159
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_fwdcolor="#00ff00"
                     /label="SV40 NLS(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        5201..5448
                     /label="3' PB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER 3' PB repeat"
                     /gene="3' PB"
                     /note="3' PB repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        complement(5489..5507)
                     /label="T3p"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER T3 promoter promoter for bacteriophage T3R NA polymerase"
                     /gene="T3p"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(5528..5544)
                     /label="M13R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="M13 rev common sequencing primer, one of multiplesi milar variants"
                     /gene="M13R"
                     /note="common sequencing primer, one of multiple similarva riants"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     rep_origin      complement(5930..6518)
                     /label="ORI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="REP_ORIGIN ori high-copy-numberColE1/pMB1/pBR322/pU C origin of replication"
                     /gene="ORI"
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofrep lication"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(6689..7549)
                     /label="AmpR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(6689..7549)
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#ffc0cb"
                     /ApEinfo_fwdcolor="#ffc0cb"
                     /note="CDS beta-lactamase AmpR confers resistance toampici llin, carbenicillin, and related antibiotics"
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,and related antibiotics"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(7550..7654)
                     /label="AmpRp"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER AmpR promoter"
                     /gene="AmpRp"
                     /note="AmpR promoter"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     rep_origin      complement(7680..8135)
                     /label="ORI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="REP_ORIGIN f1 ori f1 bacteriophage origin ofreplica tion; arrow indicates direction of (+) strandsynthesis"
                     /gene="ORI"
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrowindica tes direction of (+) strand synthesis"
                     /label="ORI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggggcgcg
       61 ccattctaga ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa
      121 tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc
      181 gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac
      241 tataacgacc gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt
      301 aactcatacg ataattatat tgttatttca tgttctactt acgtgataac ttattatata
      361 tatattttct tgttatagat atcaactaga atgctagcat gggcccagat ctcctgcagg
      421 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
      481 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
      541 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
      601 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
      661 gaagtagcac gtctcactag gctcgtgcag atggacagca ccgctgagca atggaagcgg
      721 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
      781 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
      841 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
      901 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttgtc gacgccacca
      961 tgGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA
     1021 TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC
     1081 ACAGCATCAA GAAGAACCTG ATCGGCGCCC TGCTGTTCGA CAGCGGAGAA ACAGCCGAGG
     1141 CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT
     1201 ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC
     1261 TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA
     1321 ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA
     1381 AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT CTATCTGGCC CTGGCCCACA
     1441 TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG
     1501 TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA GCTGTTCGAG GAAAACCCCA
     1561 TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC CAGACTGAGC AAGAGCAGAC
     1621 GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA GAATGGCCTG TTCGGCAACC
     1681 TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGAC CTGGCCGAGG
     1741 ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA CCTGGACAAC CTGCTGGCCC
     1801 AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA GAACCTGTCC GACGCCATCC
     1861 TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA GGCCCCCCTG AGCGCCTCTA
     1921 TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT GCTGAAAGCT CTCGTGCGGC
     1981 AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA GAGCAAGAAC GGCTACGCCG
     2041 GCTACATCGA TGGCGGAGCC AGCCAGGAAG AGTTCTACAA GTTCATCAAG CCCATCCTGG
     2101 AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA
     2161 AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT CCACCTGGGA GAGCTGCACG
     2221 CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA GGACAACCGG GAAAAGATCG
     2281 AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC TCTGGCCAGG GGAAACAGCA
     2341 GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC CCCCTGGAAC TTCGAGGAAG
     2401 TGGTGGACAA GGGCGCCAGC GCCCAGAGCT TCATCGAGCG GATGACCAAC TTCGATAAGA
     2461 ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT GTACGAGTAC TTCACCGTGT
     2521 ACAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA
     2581 GCGGCGAGCA GAAAAAAGCC ATCGTGGACC TGCTGTTCAA GACCAACCGG AAAGTGACCG
     2641 TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG CTTCGACTCC GTGGAAATCT
     2701 CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA CCACGATCTG CTGAAAATTA
     2761 TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA CATTCTGGAA GATATCGTGC
     2821 TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA ACGGCTGAAA ACCTATGCCC
     2881 ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG GAGATACACC GGCTGGGGCA
     2941 GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA GTCCGGCAAG ACAATCCTGG
     3001 ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT GCAGCTGATC CACGACGACA
     3061 GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC CGGCCAGGGC GATAGCCTGC
     3121 ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA GAAGGGCATC CTGCAGACAG
     3181 TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA CAAGCCCGAG AACATCGTGA
     3241 TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA GAAGAACAGC CGCGAGAGAA
     3301 TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA GATCCTGAAA GAACACCCCG
     3361 TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA CTACCTGCAG AATGGGCGGG
     3421 ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC CGACTACGAT GTGGACGCTA
     3481 TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGATAA CAAAGTGCTG ACTCGGAGCG
     3541 ACAAGAACCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA GGTCGTGAAG AAGATGAAGA
     3601 ACTACTGGCG CCAGCTGCTG AATGCCAAGC TGATTACCCA GAGGAAGTTC GACAATCTGA
     3661 CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC CGGCTTCATC AAGAGACAGC
     3721 TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT CCTGGACTCC CGGATGAACA
     3781 CTAAGTACGA CGAGAACGAC AAACTGATCC GGGAAGTGAA AGTGATCACC CTGAAGTCCA
     3841 AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA AGTGCGCGAG ATCAACAACT
     3901 ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG AACCGCCCTG ATCAAAAAGT
     3961 ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA GGTGTACGAC GTGCGGAAGA
     4021 TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC CAAGTACTTC TTCTACAGCA
     4081 ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA CGGCGAGATC CGGAAGCGGC
     4141 CTCTGATCGA GACAAACGGC GAAACAGGCG AGATCGTGTG GGATAAGGGC CGGGACTTTG
     4201 CCACCGTGCG GAAAGTGCTG TCTATGCCCC AAGTGAATAT CGTGAAAAAG ACCGAGGTGC
     4261 AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG GAACAGCGAC AAGCTGATCG
     4321 CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT CGACAGCCCC ACCGTGGCCT
     4381 ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC CAAGAAACTG AAGAGTGTGA
     4441 AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT CGAGAAGAAT CCCATCGACT
     4501 TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT GATCATCAAG CTGCCTAAGT
     4561 ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT GGCCTCTGCC GGCGAACTGC
     4621 AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA CTTCCTGTAC CTGGCCAGCC
     4681 ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA GAAACAGCTG TTTGTGGAAC
     4741 AGCACAAACA CTACCTGGAC GAGATCATCG AGCAGATCAG CGAGTTCTCC AAGAGAGTGA
     4801 TCCTGGCCGA CGCTAATCTG GACAAGGTGC TGAGCGCCTA CAACAAGCAC AGAGACAAGC
     4861 CTATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC CCTGACCAAT CTGGGAGCCC
     4921 CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA GAGGTACACC AGCACCAAAG
     4981 AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG CCTGTACGAG ACACGGATCG
     5041 ACCTGTCTCA GCTGGGAGGC GACGCCTATC CCTATGACGT GCCCGATTAT GCCAGCCTGG
     5101 GCAGCGGCTC CCCCAAGAAA AAACGCAAGG TGGAAGATCC TAAGAAAAAG CGGAAAGTGG
     5161 ACGGCATTGG TAGTGGGAGC AACggatgac tcgagctagt taaaagtttt gttactttat
     5221 agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt
     5281 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
     5341 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta
     5401 tctttaacgt acgtcacaat atgattatct ttctagggtt aatctagtat acgcgtatgc
     5461 ggccgcttaa ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5521 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5581 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5641 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5701 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6361 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6661 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6721 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6781 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6841 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6901 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6961 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7021 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7081 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7141 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7201 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7261 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7321 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7381 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7441 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7501 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7561 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7621 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     7681 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7741 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7801 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7861 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7921 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7981 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8041 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8101 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     8161 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8221 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgtt
//