LOCUS       pHS-MCP-VPR             6561 bp ds-DNA     circular     19-MAY-2025
DEFINITION  7930 bp ds-DNA    circular SYN 20-JAN-2014 .
COMMENT     Imported using the Genbank importer. File name:
            84239-attachment_rRoGDegu4mCD.gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label="M13F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="M13 fwd common sequencing primer, one of multiplesi milar variants"
                     /gene="M13F"
                     /note="common sequencing primer, one of multiple similarva riants"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        71..392
                     /label="5' PB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER 5' PB repeat"
                     /gene="5' PB"
                     /note="5' PB repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     enhancer        413..792
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        793..996
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     CDS             1009..2955
                     /label="MCP-VPR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYEFSSAAGTSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSPKKKRKVGSQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISSGSGSGSRDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF*"
     misc_feature    1012..1359
                     /label="MCP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1387..1499
                     /label="VP64 AD domain"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1387..2952
                     /label="VPR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1501..1542
                     /label="VP64 AD domain"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="VP64 AD domain(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1543..1563
                     /label="NLS signal"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1570..2364
                     /label="p65 AD domain"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2365..2382
                     /label="linker"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2383..2952
                     /label="RTA"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     enhancer        3000..3591
                     /label="WPRE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /vntifkey=9
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3594..3724
                     /label="SV40 PA terminator"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(3813..3829)
                     /label="M13R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="M13 rev common sequencing primer, one of multiplesi milar variants"
                     /gene="M13R"
                     /note="common sequencing primer, one of multiple similarva riants"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     rep_origin      complement(4215..4803)
                     /label="ORI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="REP_ORIGIN ori high-copy-numberColE1/pMB1/pBR322/pU C origin of replication"
                     /gene="ORI"
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofrep lication"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(4974..5834)
                     /label="AmpR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(4974..5834)
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#ffc0cb"
                     /ApEinfo_fwdcolor="#ffc0cb"
                     /note="CDS beta-lactamase AmpR confers resistance toampici llin, carbenicillin, and related antibiotics"
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,and related antibiotics"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(5835..5939)
                     /label="AmpRp"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="PROMOTER AmpR promoter"
                     /gene="AmpRp"
                     /note="AmpR promoter"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     rep_origin      complement(5965..6420)
                     /label="ORI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="REP_ORIGIN f1 ori f1 bacteriophage origin ofreplica tion; arrow indicates direction of (+) strandsynthesis"
                     /gene="ORI"
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrowindica tes direction of (+) strand synthesis"
                     /label="ORI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggggcgcg
       61 ccattctaga ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa
      121 tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc
      181 gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac
      241 tataacgacc gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt
      301 aactcatacg ataattatat tgttatttca tgttctactt acgtgataac ttattatata
      361 tatattttct tgttatagat atcaactaga atgctagcat gggcccagat ctgacattga
      421 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      661 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      721 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      781 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      841 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      901 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      961 aggcgtgtac ggtgggaggt ctatataagc agagctgaat tcgccaccat gGCTTCAAAC
     1021 TTCACGCAGT TCGTTCTTGT GGATAATGGC GGCACTGGAG ACGTTACAGT GGCTCCTAGC
     1081 AATTTCGCGA ACGGGATCGC AGAATGGATT AGCTCAAATA GTCGATCTCA GGCTTACAAA
     1141 GTGACATGTT CCGTACGGCA GTCCTCCGCG CAAAACAGGA AATATACCAT CAAAGTGGAG
     1201 GTCCCGAAAG GCGCCTGGCG ATCCTACCTG AACATGGAGC TGACCATTCC TATTTTCGCC
     1261 ACTAACTCTG ATTGCGAGCT GATCGTAAAG GCTATGCAGG GCCTGCTGAA GGATGGCAAT
     1321 CCTATACCAA GCGCCATTGC TGCAAATAGT GGCATCTATg aattcTCAAG CGCTGCAGGT
     1381 actagtGATG CTTTAGACGA TTTTGACTTA GATATGCTTG GTTCAGACGC GTTAGACGAC
     1441 TTCGACCTAG ACATGTTAGG CTCAGATGCA TTGGACGACT TCGATTTAGA TATGTTGGGC
     1501 TCCGATGCCC TAGATGACTT TGATCTAGAT ATGCTAGGTA GTCCCAAAAA GAAGAGGAAA
     1561 GTGGGATCCC AGTATCTGCC CGACACAGAT GATAGACACC GAATCGAAGA GAAACGCAAG
     1621 CGAACGTATG AAACCTTCAA ATCGATCATG AAGAAATCGC CCTTCTCGGG TCCGACCGAT
     1681 CCCAGGCCCC CACCGAGAAG GATTGCGGTC CCGTCCCGCT CGTCGGCCAG CGTGCCGAAG
     1741 CCTGCGCCGC AGCCCTACCC CTTCACGTCG AGCCTGAGCA CAATCAATTA TGACGAGTTC
     1801 CCGACGATGG TGTTCCCCTC GGGACAAATC TCACAAGCCT CGGCGCTCGC ACCAGCGCCT
     1861 CCCCAAGTCC TTCCGCAAGC GCCTGCCCCA GCGCCTGCAC CGGCAATGGT GTCCGCCCTC
     1921 GCACAGGCCC CTGCGCCCGT CCCCGTGCTC GCGCCTGGAC CGCCCCAGGC GGTCGCTCCA
     1981 CCGGCTCCGA AGCCGACGCA GGCCGGAGAG GGAACACTCT CCGAAGCACT TCTTCAACTC
     2041 CAGTTTGATG ACGAGGATCT TGGAGCACTC CTTGGAAACT CGACAGACCC TGCGGTGTTT
     2101 ACCGACCTCG CGTCAGTAGA TAACTCCGAA TTTCAGCAGC TTTTGAACCA GGGTATCCCG
     2161 GTCGCGCCAC ATACAACGGA GCCCATGTTG ATGGAATACC CCGAAGCAAT CACGAGACTT
     2221 GTGACGGGAG CGCAGCGGCC TCCCGATCCC GCACCCGCAC CTTTGGGGGC ACCTGGCCTC
     2281 CCTAACGGAC TTTTGAGCGG CGACGAGGAT TTCTCCTCCA TCGCCGATAT GGATTTCTCA
     2341 GCCTTGCTGT CACAGATTTC CAGCGGCTCT GGCAGCGGCA GCCGGGATTC CAGGGAAGGG
     2401 ATGTTTTTGC CGAAGCCTGA GGCCGGCTCC GCTATTAGTG ACGTGTTTGA GGGCCGCGAG
     2461 GTGTGCCAGC CAAAACGAAT CCGGCCATTT CATCCTCCAG GAAGTCCATG GGCCAACCGC
     2521 CCACTCCCCG CCAGCCTCGC ACCAACACCA ACCGGTCCAG TACATGAGCC AGTCGGGTCA
     2581 CTGACCCCGG CACCAGTCCC TCAGCCACTG GATCCAGCGC CCGCAGTGAC TCCCGAGGCC
     2641 AGTCACCTGT TGGAGGATCC CGATGAAGAG ACGAGCCAGG CTGTCAAAGC CCTTCGGGAG
     2701 ATGGCCGATA CTGTGATTCC CCAGAAGGAA GAGGCTGCAA TCTGTGGCCA AATGGACCTT
     2761 TCCCATCCGC CCCCAAGGGG CCATCTGGAT GAGCTGACAA CCACACTTGA GTCCATGACC
     2821 GAGGATCTGA ACCTGGACTC ACCCCTGACC CCGGAATTGA ACGAGATTCT GGATACCTTC
     2881 CTGAACGACG AGTGCCTCTT GCATGCCATG CATATCAGCA CAGGACTGTC CATCTTCGAC
     2941 ACATCTCTGT TTTGAattta aatgtttaaa ccatgcaagc ttgatatcaa gcttatcgat
     3001 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3061 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3361 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3421 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3541 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tccttgttta
     3601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3721 ggatcctgca ggtatacgcg tatgcggccg cttaattaat ccagcttttg ttccctttag
     3781 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3841 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3901 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3961 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4021 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4081 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4141 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4621 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4981 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5041 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5101 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5161 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5221 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5281 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5341 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5581 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5941 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     6001 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6061 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6121 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6181 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6241 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6301 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6361 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6421 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6481 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6541 ggttttccca gtcacgacgt t
//