LOCUS       VB240221-1672qqx       10759 bp    DNA     circular SYN 20-MAY-2025
DEFINITION  
ACCESSION   VB240221-1672qqx
VERSION     VB240221-1672qqx
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..10759
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /label="RSV  promoter"
                     /note="color: #d05c0a; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /label="5' LTR-ΔU3"
                     /note="color: #fd3434; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
     Miscellaneous   521..565
                     /application_notes="Allows packaging of viral RNA into
                     virus."
                     /description="HIV-1 packaging signal"
                     /full_name="HIV-1 psi packaging signal"
                     /label="Ψ"
                     /note="color: #46c6ef; direction: RIGHT"
                     /official_designation="Psi"
                     /uuid="284899413ff943a99f8210a11ad8c54e"
     Miscellaneous   1075..1308
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /label="RRE"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
     Miscellaneous   1803..1920
                     /application_notes="Facilitates the nuclear import of HIV-1
                     cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /label="cPPT"
                     /note="color: #da3bb5; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
     Promoter        1959..2557
                     /application_notes=""
                     /label="{hTNNT2 promoter}"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="{hTNNT2 promoter}"
                     /uuid="b6cd252d75fe42bbb0ff6e0c0f71d18e"
     Miscellaneous   2582..2587
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             2588..5005
                     /application_notes=""
                     /label="{FUCCIMOX}"
                     /note="color: #5cad08; direction: RIGHT"
                     /official_designation="{FUCCIMOX}"
                     /uuid="fda1294cdd104553b96340e8035a5af9"
     Miscellaneous   5044..5641
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     Promoter        5660..6170
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /label="mPGK promoter"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
     CDS             6183..7208
                     /application_notes="Allows cells to be resistant to
                     hygromycin B."
                     /description="Hygromycin resistance gene"
                     /full_name="Hygromycin resistance gene"
                     /label="Hygro"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Hygro"
                     /uuid="9135dd0aa64645f3aa3c381325794022"
     LTR             7279..7513
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /label="3' LTR-ΔU3"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
     PolyA_signal    7586..7720
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation signal"
                     /full_name="SV40 early polyadenation signal"
                     /label="SV40 early pA"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
     CDS             8674..9534
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
     Rep_origin      9705..10293
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #a949ca; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggt catggagaag acccaccttg
     1981 cagatgtcct cactggggct ggcagagccg gcaacctgcc taaggctgct cagtccatta
     2041 ggagccagta gcctggaaga tgtctttacc cccagcatca gttcaagtgg agcagcacat
     2101 aactcttgcc ctctgccttc caagattctg gtgctgagac ttatggagtg tcttggaggt
     2161 tgccttctgc cccccaaccc tgctcccagc tggccctccc aggcctgggt tgctggcctc
     2221 tgctttatca ggattctcaa gagggacagc tggtttatgt tgcatgactg ttccctgcat
     2281 atctgctctg gttttaaata gcttatctga gcagctggag gaccacatgg gcttatatgg
     2341 cgtggggtac atgttcctgt agccttgtcc ctggcacctg ccaaaatagc agccaacacc
     2401 ccccaccccc accgccatcc ccctgcccca cccgtcccct gtcgcacatt cctccctccg
     2461 cagggctggc tcaccaggcc ccagcccaca tgcctgctta aagccctctc catcctctgc
     2521 ctcacccagt ccccgctgag actgagcaga cgcctcccaa gtttgtacaa aaaagcaggc
     2581 tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actactttag cgacaacgtc tatatcaccg ccgacaagca
     3061 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagtacagc atgctgccta gcctcggcgg ctaccctagc cattggaggc ccctggaacc
     3361 ctctcctgcc agacctgccc tgagagcccc tgcctccgcc acctccggca gcagaaagag
     3421 agccagacct cctgccgccc ctggcagaga tcaggccagg ccccctgccc gcagacggct
     3481 ccggctgtcc gtggatgagg tgtccagccc tagcaccccc gaggcccccg atatccctgc
     3541 ctgtcccagc cctggccaga aaatcaagaa gagcacacct gccgccggac agcctcctca
     3601 cctgaccagc gcccaggacc aggacaccat cgtcgacgag ggcaggggca gcctgctgac
     3661 ctgcggcgac gtggaggaga accccggccc cagatccatg gtagcaggtc atgcctctgg
     3721 cagccccgca ttcgggaccg cctctcattc gaattgcgaa catgaagaga tccacctcgc
     3781 cggctcgatc cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat
     3841 ccaggccagc gccaacgccg cggaatttct gaatctcgga agcgtactcg gcgttccgct
     3901 cgccgagatc gacggcgatc tgttgatcaa gatcctgccg catctcgatc ccaccgccga
     3961 aggcatgccg gtcgcggtgc gctgccggat cggcaatccc tctacggagt actgcggtct
     4021 gatgcatcgg cctccggaag gcgggctgat catcgaactc gaacgtgccg gcccgtcgat
     4081 cgatctgtca ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg gttcactgcg
     4141 cgcgctgtgc gatgacaccg tgctgctgtt tcagcagtgc accggctacg accgggtgat
     4201 ggtgtatcgt ttcgatgagc aaggccacgg cctggtattc tccgagtgcc atgtgcctgg
     4261 gctcgaatcc tatttcggca accgctatcc gtcgtcgact gtcccgcaga tggcgcggca
     4321 gctgtacgtg cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct
     4381 ggagccgcgg ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg
     4441 ctcgatgtcg ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc
     4501 ggtgtcgctg gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc attatctgcc
     4561 gcgcttcatc cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac
     4621 gcggatcacc gcgcttgaga gcggctaccc cagccactgg cggcctctgg aaatgaaccc
     4681 cagcatgaag cagaagcagg aagagatcaa agagaacatc aagaacagca gcgtgcccag
     4741 gcggaccctg aagatgatcc agcccagcgc cagcggatct ctggtgggcc gggagaatga
     4801 gctgtctgcc ggcctgagca agcggaagca ccggaacgac cacctgacca gcaccacctc
     4861 tagcccaggc gtgatcgtgc ccgagagcag cgagaacaag aacctgggcg gcgtgaccca
     4921 agaaagcttc gacctgatga tcaaagaaaa ccccagcagc cagtactgga aagaggtggc
     4981 cgagaagcgg cggaaggccc tgtaaaccca gctttcttgt acaaagtggt gataatcgaa
     5041 ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5101 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5161 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5221 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5281 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5341 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5401 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5461 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5521 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5581 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5641 ggaattcccg cggttcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5701 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5761 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5821 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5881 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     5941 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     6001 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     6061 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     6121 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct cacgtggcca
     6181 ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     6241 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     6301 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     6361 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     6421 ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     6481 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     6541 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     6601 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     6661 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     6721 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     6781 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     6841 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     6901 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     6961 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     7021 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     7081 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     7141 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     7201 aggaataggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7261 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7321 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7501 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7561 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7681 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7801 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7861 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     7921 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7981 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8041 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8161 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8281 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8401 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8461 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8521 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8641 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8701 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8761 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8821 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8881 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8941 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9001 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9061 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9121 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9181 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9241 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9301 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9361 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9421 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9481 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9541 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9601 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9721 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9781 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9841 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9901 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9961 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10021 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10081 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg
    10141 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10201 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10261 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10321 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10381 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10441 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10501 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10561 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10621 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10681 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
    10741 aagctggagc tgcaagctt
//
