LOCUS       VB240216-1127yus       10749 bp    DNA     circular SYN 20-MAY-2025
DEFINITION  
ACCESSION   VB240216-1127yus
VERSION     VB240216-1127yus
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..10749
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /label="RSV  promoter"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /label="5' LTR-ΔU3"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
     Miscellaneous   521..565
                     /application_notes="Allows packaging of viral RNA into
                     virus."
                     /description="HIV-1 packaging signal"
                     /full_name="HIV-1 psi packaging signal"
                     /label="Ψ"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Psi"
                     /uuid="284899413ff943a99f8210a11ad8c54e"
     Miscellaneous   1075..1308
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /label="RRE"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
     Miscellaneous   1803..1920
                     /application_notes="Facilitates the nuclear import of HIV-1
                     cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /label="cPPT"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
     Promoter        1959..2547
                     /application_notes="Strong promoter; may have variable
                     strength in some cell types."
                     /description="Human cytomegalovirus immediate early
                     enhancer/promoter"
                     /full_name="Human cytomegalovirus immediate early promoter"
                     /label="CMV"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="CMV"
                     /uuid="bbb1016d51c4484eaa5a960df4edf21a"
     Miscellaneous   2572..2577
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #a949ca; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             2578..4995
                     /application_notes=""
                     /label="{FUCCIMOX}"
                     /note="color: #68e66d; direction: RIGHT"
                     /official_designation="{FUCCIMOX}"
                     /uuid="fda1294cdd104553b96340e8035a5af9"
     Miscellaneous   5034..5631
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     Promoter        5650..6160
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /label="mPGK promoter"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
     CDS             6173..7198
                     /application_notes="Allows cells to be resistant to
                     hygromycin B."
                     /description="Hygromycin resistance gene"
                     /full_name="Hygromycin resistance gene"
                     /label="Hygro"
                     /note="color: #d84e4e; direction: RIGHT"
                     /official_designation="Hygro"
                     /uuid="9135dd0aa64645f3aa3c381325794022"
     LTR             7269..7503
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /label="3' LTR-ΔU3"
                     /note="color: #1f36a9; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
     PolyA_signal    7576..7710
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation signal"
                     /full_name="SV40 early polyadenation signal"
                     /label="SV40 early pA"
                     /note="color: #db6901; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
     CDS             8664..9524
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #800c0c; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
     Rep_origin      9695..10283
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #0c5d90; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2641 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2701 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2761 gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     2821 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2881 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2941 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3001 ctggagtaca actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     3061 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     3121 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3181 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3241 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtacagc
     3301 atgctgccta gcctcggcgg ctaccctagc cattggaggc ccctggaacc ctctcctgcc
     3361 agacctgccc tgagagcccc tgcctccgcc acctccggca gcagaaagag agccagacct
     3421 cctgccgccc ctggcagaga tcaggccagg ccccctgccc gcagacggct ccggctgtcc
     3481 gtggatgagg tgtccagccc tagcaccccc gaggcccccg atatccctgc ctgtcccagc
     3541 cctggccaga aaatcaagaa gagcacacct gccgccggac agcctcctca cctgaccagc
     3601 gcccaggacc aggacaccat cgtcgacgag ggcaggggca gcctgctgac ctgcggcgac
     3661 gtggaggaga accccggccc cagatccatg gtagcaggtc atgcctctgg cagccccgca
     3721 ttcgggaccg cctctcattc gaattgcgaa catgaagaga tccacctcgc cggctcgatc
     3781 cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat ccaggccagc
     3841 gccaacgccg cggaatttct gaatctcgga agcgtactcg gcgttccgct cgccgagatc
     3901 gacggcgatc tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg
     3961 gtcgcggtgc gctgccggat cggcaatccc tctacggagt actgcggtct gatgcatcgg
     4021 cctccggaag gcgggctgat catcgaactc gaacgtgccg gcccgtcgat cgatctgtca
     4081 ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg gttcactgcg cgcgctgtgc
     4141 gatgacaccg tgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgt
     4201 ttcgatgagc aaggccacgg cctggtattc tccgagtgcc atgtgcctgg gctcgaatcc
     4261 tatttcggca accgctatcc gtcgtcgact gtcccgcaga tggcgcggca gctgtacgtg
     4321 cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct ggagccgcgg
     4381 ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg
     4441 ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc ggtgtcgctg
     4501 gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc attatctgcc gcgcttcatc
     4561 cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac gcggatcacc
     4621 gcgcttgaga gcggctaccc cagccactgg cggcctctgg aaatgaaccc cagcatgaag
     4681 cagaagcagg aagagatcaa agagaacatc aagaacagca gcgtgcccag gcggaccctg
     4741 aagatgatcc agcccagcgc cagcggatct ctggtgggcc gggagaatga gctgtctgcc
     4801 ggcctgagca agcggaagca ccggaacgac cacctgacca gcaccacctc tagcccaggc
     4861 gtgatcgtgc ccgagagcag cgagaacaag aacctgggcg gcgtgaccca agaaagcttc
     4921 gacctgatga tcaaagaaaa ccccagcagc cagtactgga aagaggtggc cgagaagcgg
     4981 cggaaggccc tgtaaaccca gctttcttgt acaaagtggt gataatcgaa ttccgataat
     5041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5401 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcccg
     5641 cggttcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5701 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5761 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5821 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5881 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5941 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     6001 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     6061 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6121 gcgctgttct cctcttcctc atctccgggc ctttcgacct cacgtggcca ccatgaaaaa
     6181 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     6241 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     6301 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     6361 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     6421 tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     6481 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     6541 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     6601 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     6661 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     6721 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     6781 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     6841 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     6901 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     6961 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     7021 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     7081 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     7141 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataggg
     7201 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7261 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7321 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7501 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7561 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7681 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7741 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7861 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7921 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7981 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8041 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8281 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8401 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8521 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8581 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8641 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8701 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8761 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8821 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8881 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8941 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9001 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9061 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9121 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9181 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9241 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9301 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9361 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9421 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9481 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9541 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9601 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9661 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9721 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9781 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9841 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9901 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9961 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10081 gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa
    10141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10381 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10441 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10501 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10561 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10621 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10681 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10741 tgcaagctt
//
