LOCUS       pUAS-spatzle-HA_        8647 bp DNA     circular SYN 09-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8647
                     /label=Overexpression vector pVALIUM10-roe
                     /organism="synthetic DNA construct"
     misc_feature    664..2543
                     /label=vermillion
                     /note="vermillion"
     CDS             complement(join(834..951,1028..1121,1213..1819,1874..2007,
                     2068..2228,2302..2327))
                     /codon_start=1
                     /label=vermillion
                     /note="selectable marker"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     misc_recomb     2568..2935
                     /label=attB docking site
                     /note="attB docking site"
     misc_feature    2901..2920
                     /label=attB_seq_1F
                     /note="attB_seq_1F"
     misc_feature    2947..3377
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_recomb     3395..3428
                     /label=LoxP
                     /note="LoxP"
     misc_feature    3435..3544
                     /label=5XUAS
                     /note="5XUAS"
     misc_recomb     3551..3584
                     /label=LoxP
                     /note="LoxP"
     misc_feature    3591..3700
                     /label=5XUAS
                     /note="5XUAS"
     regulatory      3707..3966
                     /label=promoter
                     /note="Hsp70 promoter"
     misc_recomb     3994..5112
                     /label=attR; gateway recombination
                     /note="attR; gateway recombination"
     misc_feature    4006..5101
                     /label=paste into expression clone
                     /note="paste into expression clone"
     misc_feature    4006..4022
                     /label=attL1
                     /note="attL1"
     misc_feature    4023..4042
                     /label=overlap
                     /note="overlap"
     misc_feature    4043..4969
                     /label=linker
                     /note="linker"
     misc_feature    4043..4942
                     /label=spatzle CDS
     misc_feature    4625..4942
                     /label=cleaved form
     misc_feature    4970..4996
                     /label=HA
                     /note="HA"
     misc_feature    4997..5014
                     /label=linker
                     /note="linker"
     misc_feature    5015..5062
                     /label=spGFP11
                     /note="spGFP11"
     misc_feature    5069..5088
                     /label=overlap
                     /note="overlap"
     misc_feature    5085..5101
                     /label=attL2
                     /note="attL2"
     intron          5170..5316
                     /label=ftz intron
                     /note="ftz intron"
     misc_feature    5340..6039
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     misc_feature    6046..6476
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_feature    7698..8357
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttatttatt ttgttatgtt atatgtatta tatgtcagac ataaagaaaa ggaacacatc
      721 aaatgtgata acaaagacta aacaagtaat tttattacac caaaacgaca aaacagtagg
      781 cagaacaaac aacgcatagc caaacattga cgaattggat accctgccga ttgtcagaca
      841 cttttgttga tcagtttctt gcgaatggtc tcgtccagcg gtggaatcgc ctcgcgggga
      901 atcagaaaag tggacagatt gaacagatcc agaaacacct tgtaccgatc actgaaacca
      961 aaaaaaaaca aagggagaac agtttgagtt cattgatccc cgatataatc acatctgcga
     1021 tgatcacctg agagtggagc gcagatattg atatccagac gagccaccag tgcccaactg
     1081 ttgggatcca atcatgcgtt gcaccatgat cacgtgattg tctgcggcgg gaatagaaag
     1141 tatttggtta ggaaaaccag tcttaaacat aagatatatt tataaaagag tatcaaagaa
     1201 tgcaatactt acatctccac ttggttatta acgagtcgat gtccatgagc agggtgagca
     1261 actggtgtgg ttggctgaac ctgggttcat ccctatagaa ggtgatcatg atggctccct
     1321 gaagggcacg atggctaaac cggcgatccc cacgacgcac cagtgcatcg tgcactgccg
     1381 gatcaaagat ggagcgatac acctcgcgtc gcttctcaat gtccatgagg cggtagtttt
     1441 tcgccttctc cacgggctcc tccatggcgc tctgtacctg cgcctccagg aatcgatcga
     1501 cgctctcctg aaacttggcc cagaagttga agccactctc ctccagtccg ggcgtcctct
     1561 ccagccatcg ctgcactagc tccagtagcg agggatcttt ctccgagttg cgaatcgagt
     1621 tccgcgcctc ctcgtcgcta aagacatccg agtacttctg gttgtatctc acccgctgct
     1681 ctgtcagaac tcccagcttg ttctcgatca aacggaactg cagcgactga aaaccagatg
     1741 cgggtgccag gtacttgcgg aagtccatga agtctagcgg ggtcatggtc tccagaatgg
     1801 gcacttggtc caccaggagc tgtacaaagg aagttataaa cggattttgg taagagattc
     1861 agaaagcact cacttttaga atcagaacca ctcggttcag tcgcttgaca atctccagcg
     1921 tcttggtttc atcgatgacc tctgcatcca acatgtctcg tatggagtcg aactcaaaga
     1981 tgatctgctt gaaccaaagc tcgtaggctg tggcgaaggt acttaaatgc cattgagtgt
     2041 tgtcatcaaa gttgtaaacc tactcaccct ggtgcgtgat gatgaacaga tgctcatcgt
     2101 gcacgggtcg cttgtcctcc tcggacagca tacactgggc atccagcagt ttgtccagca
     2161 tcagatactc tccatagatt ttgcccactt ccgtggttaa tggcaccgcc gaatcatcgt
     2221 gatcgtttct gtatgggttt gaattgaatc gcagaactga agatcgattg gcattcctgg
     2281 acagcacgtg ctggtgctca cccgtttcct gcatagggac agctcatggt gcacagctca
     2341 gatcagatcg tgactcctcg agcggcggat gctggcgaac tgatctccgc cagcggaccg
     2401 gagatgagac cccagcgaac cgataacaga gcgagagagc tccagttccg actgattgca
     2461 cagtcggtga tctgggcgat gggcactgcc agataggctg ggaattatca atcacttgag
     2521 gtgaaagtgc ggcgcacaca aataagcttg atatcatcga tctcgaggct gcatccaacg
     2581 cgttgggagc tctccggatc aattcggctt caggtaccgt cgacgatgta ggtcacggtc
     2641 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     2701 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     2761 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     2821 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     2881 acggtcacgg cgggcatgtc gacaagccga attgatccac tagaaggcct aattcggtac
     2941 actagttggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     3001 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     3061 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     3121 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     3181 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat
     3241 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     3301 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3361 gtgtgccaac caacaacact agtagtacca gcttataact tcgtataatg tatgctatac
     3421 gaagttatct gcaggcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
     3481 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3541 actcccatgg ataacttcgt ataatgtatg ctatacgaag ttatggatcc gcaggtcgga
     3601 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3661 ctgtcctccg agcggagtac tgtcctccga gcggagactc gtcgacagcg agcgccggag
     3721 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3781 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3841 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3901 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3961 aattgggaat tccagatctg cggccgcggc tcgaatcaca agtttgtaca aaaaagcagg
     4021 ctccgcggcc gcccccttca ccatgatgac gcccatgtgg atatcgctgt tcaaagtcct
     4081 gctgctgctg ttcgccttct ttgccacgac gtccgcggac agtgcaccct tcatgcccat
     4141 acccacccag cacgacgatc cgacccagaa acagaaacag aatcagaatc aatctccgat
     4201 acccgaaacg aaccgccatt accatcagta tcacagcctg atccaaccag atcagtactt
     4261 caaagtgcag cgctccccga atggaaagct gaatctggtc tttaacgata cgttcgtatc
     4321 tttgcagagg acggacacgg aggtgcaatc cgaacagccg ataccccccc gacatccatc
     4381 ggataccttt gtcttccccg attcccccat agccaagtat cggccaccac aatccccagc
     4441 tcgtcctttg aggaatgaca ctaaggagca caatccctgc gccaaggatg aaagccaaca
     4501 ccttagaaac ttctgcacga atgtggacga ctatccggac ctttcaggcc tcacacataa
     4561 gctaaaaaac aacttcgcca agttctttag caacgatctt cagcccacgg atgtgagctc
     4621 tcgcgttggt ggctcagatg agcgattcct ttgcaggagc atcaggaagc tggtgtaccc
     4681 aaaaaagggc ttgagggcgg acgacacctg gcagttaatt gtcaataacg atgagtacaa
     4741 acaggccatc cagatcgaag agtgcgaagg agcggatcaa ccctgtgact ttgccgccaa
     4801 ctttccgcag agctataatc caatctgcaa gcagcactac acacagcaga ccctggccag
     4861 catcaagagt gatggcgaac tggacgtggt gcagaattcc ttcaagatcc cctcctgctg
     4921 caagtgcgcg ttgaagactg ggggtgatgg tggctcgggt gggggaagtt acccatacga
     4981 tgttccagat tacgctggag gctccggtac tagccgtgac cacatggtcc ttcatgagta
     5041 tgtaaatgct gctgggatta cataatagaa gggtgggcgc gccgacccag ctttcttgta
     5101 caaagtggtg attcgagggt acctctagag caaactagtt ctgatctgct agacaattgt
     5161 tggcatcagg taggcatcac acacgattaa caacccctaa aaatacactt tgaaaatatt
     5221 gaaaatatgt ttttgtatac atttttgata ttttcaaaca atacgcagtt ataaaactca
     5281 ttagctaacc cattttttct ttgcttatgc ttacagattg caaagaacta gagccgcggg
     5341 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     5401 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     5461 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     5521 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     5581 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     5641 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     5701 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     5761 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     5821 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5881 aatgaatgga attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6001 gtccaaactc atcaatgtat cttatcatgt ctggttccag agctctggcc acgtaataag
     6061 tgtgcgttga atttattcgc aaaaacattg catattttcg gcaaagtaaa attttgttgc
     6121 ataccttatc aaaaaataag tgctgcatac tttttagaga aaccaaataa ttttttattg
     6181 catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat attgcatact
     6241 ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc atacccgttt
     6301 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatacgt tgacgaaaca
     6361 aattttcgtt gcatacccaa taaaagatta ttatattgca taccttttct tgccatacca
     6421 tttagccgat caattgtgct cggcaacagt atatttgtgg tgtgccaacc aacaacgagc
     6481 tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     6541 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6601 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6661 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6721 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7801 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8641 aaacaaa
//
