LOCUS       pUAS-hhN-HA_pVal        8518 bp DNA     circular SYN 09-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8518
                     /label=Overexpression vector pVALIUM10-roe
                     /organism="synthetic DNA construct"
     misc_feature    664..2543
                     /label=vermillion
                     /note="vermillion"
     CDS             complement(join(834..951,1028..1121,1213..1819,1874..2007,
                     2068..2228,2302..2327))
                     /codon_start=1
                     /label=vermillion
                     /note="selectable marker"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     misc_recomb     2568..2935
                     /label=attB docking site
                     /note="attB docking site"
     misc_feature    2901..2920
                     /label=attB_seq_1F
                     /note="attB_seq_1F"
     misc_feature    2947..3377
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_recomb     3395..3428
                     /label=LoxP
                     /note="LoxP"
     misc_feature    3435..3544
                     /label=5XUAS
                     /note="5XUAS"
     misc_recomb     3551..3584
                     /label=LoxP
                     /note="LoxP"
     misc_feature    3591..3700
                     /label=5XUAS
                     /note="5XUAS"
     regulatory      3707..3966
                     /label=promoter
                     /note="Hsp70 promoter"
     misc_recomb     3994..4983
                     /label=attR; gateway recombination
                     /note="attR; gateway recombination"
     misc_feature    4006..4972
                     /label=paste into expression clone
                     /note="paste into expression clone"
     misc_feature    4006..4022
                     /label=attL1
                     /note="attL1"
     misc_feature    4023..4042
                     /label=overlap
                     /note="overlap"
     misc_feature    4043..4840
                     /label=linker
                     /note="linker"
     misc_feature    4043..4813
                     /label=N-terminal
                     /note="N-terminal"
     misc_feature    4813
                     /label=cleavage
                     /note="cleavage"
     misc_feature    4841..4867
                     /label=HA
                     /note="HA"
     misc_feature    4868..4885
                     /label=linker
                     /note="linker"
     misc_feature    4886..4933
                     /label=spGFP11
                     /note="spGFP11"
     misc_feature    4940..4959
                     /label=overlap
                     /note="overlap"
     misc_feature    4956..4972
                     /label=attL2
                     /note="attL2"
     intron          5041..5187
                     /label=ftz intron
                     /note="ftz intron"
     misc_feature    5211..5910
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     misc_feature    5917..6347
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_feature    7569..8228
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttatttatt ttgttatgtt atatgtatta tatgtcagac ataaagaaaa ggaacacatc
      721 aaatgtgata acaaagacta aacaagtaat tttattacac caaaacgaca aaacagtagg
      781 cagaacaaac aacgcatagc caaacattga cgaattggat accctgccga ttgtcagaca
      841 cttttgttga tcagtttctt gcgaatggtc tcgtccagcg gtggaatcgc ctcgcgggga
      901 atcagaaaag tggacagatt gaacagatcc agaaacacct tgtaccgatc actgaaacca
      961 aaaaaaaaca aagggagaac agtttgagtt cattgatccc cgatataatc acatctgcga
     1021 tgatcacctg agagtggagc gcagatattg atatccagac gagccaccag tgcccaactg
     1081 ttgggatcca atcatgcgtt gcaccatgat cacgtgattg tctgcggcgg gaatagaaag
     1141 tatttggtta ggaaaaccag tcttaaacat aagatatatt tataaaagag tatcaaagaa
     1201 tgcaatactt acatctccac ttggttatta acgagtcgat gtccatgagc agggtgagca
     1261 actggtgtgg ttggctgaac ctgggttcat ccctatagaa ggtgatcatg atggctccct
     1321 gaagggcacg atggctaaac cggcgatccc cacgacgcac cagtgcatcg tgcactgccg
     1381 gatcaaagat ggagcgatac acctcgcgtc gcttctcaat gtccatgagg cggtagtttt
     1441 tcgccttctc cacgggctcc tccatggcgc tctgtacctg cgcctccagg aatcgatcga
     1501 cgctctcctg aaacttggcc cagaagttga agccactctc ctccagtccg ggcgtcctct
     1561 ccagccatcg ctgcactagc tccagtagcg agggatcttt ctccgagttg cgaatcgagt
     1621 tccgcgcctc ctcgtcgcta aagacatccg agtacttctg gttgtatctc acccgctgct
     1681 ctgtcagaac tcccagcttg ttctcgatca aacggaactg cagcgactga aaaccagatg
     1741 cgggtgccag gtacttgcgg aagtccatga agtctagcgg ggtcatggtc tccagaatgg
     1801 gcacttggtc caccaggagc tgtacaaagg aagttataaa cggattttgg taagagattc
     1861 agaaagcact cacttttaga atcagaacca ctcggttcag tcgcttgaca atctccagcg
     1921 tcttggtttc atcgatgacc tctgcatcca acatgtctcg tatggagtcg aactcaaaga
     1981 tgatctgctt gaaccaaagc tcgtaggctg tggcgaaggt acttaaatgc cattgagtgt
     2041 tgtcatcaaa gttgtaaacc tactcaccct ggtgcgtgat gatgaacaga tgctcatcgt
     2101 gcacgggtcg cttgtcctcc tcggacagca tacactgggc atccagcagt ttgtccagca
     2161 tcagatactc tccatagatt ttgcccactt ccgtggttaa tggcaccgcc gaatcatcgt
     2221 gatcgtttct gtatgggttt gaattgaatc gcagaactga agatcgattg gcattcctgg
     2281 acagcacgtg ctggtgctca cccgtttcct gcatagggac agctcatggt gcacagctca
     2341 gatcagatcg tgactcctcg agcggcggat gctggcgaac tgatctccgc cagcggaccg
     2401 gagatgagac cccagcgaac cgataacaga gcgagagagc tccagttccg actgattgca
     2461 cagtcggtga tctgggcgat gggcactgcc agataggctg ggaattatca atcacttgag
     2521 gtgaaagtgc ggcgcacaca aataagcttg atatcatcga tctcgaggct gcatccaacg
     2581 cgttgggagc tctccggatc aattcggctt caggtaccgt cgacgatgta ggtcacggtc
     2641 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     2701 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     2761 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     2821 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     2881 acggtcacgg cgggcatgtc gacaagccga attgatccac tagaaggcct aattcggtac
     2941 actagttggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     3001 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     3061 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     3121 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     3181 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat
     3241 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     3301 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3361 gtgtgccaac caacaacact agtagtacca gcttataact tcgtataatg tatgctatac
     3421 gaagttatct gcaggcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
     3481 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3541 actcccatgg ataacttcgt ataatgtatg ctatacgaag ttatggatcc gcaggtcgga
     3601 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3661 ctgtcctccg agcggagtac tgtcctccga gcggagactc gtcgacagcg agcgccggag
     3721 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3781 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3841 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3901 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3961 aattgggaat tccagatctg cggccgcggc tcgaatcaca agtttgtaca aaaaagcagg
     4021 ctccgcggcc gcccccttca ccatggataa ccacagctca gtgccttggg ccagtgccgc
     4081 cagtgtcacc tgtctctccc tggatgccaa atgccacagt tccagttcca gttccagctc
     4141 caaatccgca gcgagctcca tctccgcaat cccgcaagaa gaaacgcaaa cgatgcgcca
     4201 cattgcgcat acgcagcgtt gcctcagcag gctgacctct ctggtggccc tgctgctgat
     4261 cgtcttgccg atggtcttta gcccggctca cagctgcggt cctggccgag gattgggtcg
     4321 tcatagggcg cgcaacctgt atccgctggt cctcaagcag acaattccca atctatccga
     4381 gtacacgaac agcgcctccg gacctctgga gggtgtgatc cgtcgggact cgcccaaatt
     4441 caaggacctc gtgcccaact acaacaggga catccttttc cgcgacgagg aaggcaccgg
     4501 agcggatcgc ttgatgagca agcgctgcaa ggagaagcta aacgtgctgg cctactcggt
     4561 gatgaacgaa tggcccggca tccggctgct ggtcaccgag agctgggacg aggactacca
     4621 tcacggccag gagtcgctcc actacgaggg ccgagcggtg accattgcca cctccgatcg
     4681 cgaccagtcc aaatacggca tgctcgctcg cctggccgtc gaggctggat tcgattgggt
     4741 ctcctacgtc agcaggcgcc acatctactg ctccgtcaag tcagattcgt cgatcagttc
     4801 ccacgtgcac ggcggtgatg gtggctcggg tgggggaagt tacccatacg atgttccaga
     4861 ttacgctgga ggctccggta ctagccgtga ccacatggtc cttcatgagt atgtaaatgc
     4921 tgctgggatt acataataga agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     4981 gattcgaggg tacctctaga gcaaactagt tctgatctgc tagacaattg ttggcatcag
     5041 gtaggcatca cacacgatta acaaccccta aaaatacact ttgaaaatat tgaaaatatg
     5101 tttttgtata catttttgat attttcaaac aatacgcagt tataaaactc attagctaac
     5161 ccattttttc tttgcttatg cttacagatt gcaaagaact agagccgcgg gatctttgtg
     5221 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     5281 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     5341 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     5401 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     5461 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     5521 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     5581 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     5641 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     5701 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgg
     5761 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5821 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5881 catcaatgta tcttatcatg tctggttcca gagctctggc cacgtaataa gtgtgcgttg
     5941 aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat
     6001 caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt
     6061 ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa
     6121 caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa
     6181 tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt
     6241 tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga
     6301 tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacgag ctccagcttt
     6361 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     6421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaa
//
