LOCUS       pHIV-IL13-TetCoi        8920 bp    DNA     circular SYN 21-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        join(2624..3589,3590..3802)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="This forward directional feature has 2 segments:
                        1: 2624 .. 3589 / #ffffff
                        2: 3590 .. 3802 / #ffffff"
     intron          join(2855..3589,3590..3793)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="This feature has 2 segments:
                        1: 2855 .. 3589 / #------
                        2: 3590 .. 3793 / #------"
     primer_bind     complement(3424..3436)
                     /label=WPRE-R
                     /note="color: black; sequence: 
                     TATCGATGCGGCCGCTTTACTTgcggggaggcggc"
     primer_bind     3586..3612
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: GTTCTCGAGCTTTTGGAGTACGTCGTC"
     primer_bind     3782..3811
                     /label=pHIV_clone_f
                     /note="color: black; sequence: 
                     CTTCCATTTCAGGTGTCGTGAGCGGCCGCT"
     primer_bind     complement(3802..3811)
                     /label=pHIV_clone_f
                     /note="color: black; sequence: 
                     CTTCCATTTCAGGTGTCGTGAGCGGCCGCT"
     CDS             3835..3894
                     /codon_start=1
                     /label=IgK Leader
                     /note="color: #993366"
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3895..4230
                     /codon_start=1
                     /label=IL13
                     /note="color: #993366"
                     /translation="GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCA
                     ALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKK
                     LFREGRFN"
     CDS             4231..4257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             4258..4278
                     /label=Linker
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4279..4374
                     /label=CC-Tet
                     /note="color: #339966"
     CDS             4375..4467
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /note="color: #993366; direction: RIGHT"
     primer_bind     4378..4400
                     /label=PDGFR_AscI_link_f
                     /note="color: black; sequence: 
                     GGTGGAAGCGGAGGCGGTGGATCTGCTAGCGTGGTGGTGATCTCAGCCATCCT"
     primer_bind     complement(4407..4470)
                     /label=PDGFR_r3
                     /note="color: black; sequence: 
                     TCAACGTGGCTTCTTCTGCCAAAGCATGATGAGGATGATAAGGGAGATGATGGTGAGCA
                     CCACC"
     primer_bind     complement(4443..4507)
                     /label=pHIVW_PDGFR_gib_r
                     /note="color: black; sequence: 
                     GGGGGGGGGGAGGGAGAGGGGCGGATCCTCAGAATTCTCAACGTGGCTTCTTCTGCCAA
                     AGCATG"
     primer_bind     complement(4443..4487)
                     /label=pHIVW_gib2_r1
                     /note="color: black; sequence: 
                     GGGTACTCTAGATCAGCTAGCGAATCCTCCGAATTCTCAACGTGGCTTCTTCTGCCAAA
                     GCATG"
     misc_feature    join(4487..5061,5062..5064,5065..5073)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 4487 .. 5061 / #a6acb3
                        2: 5062 .. 5064 / #a6acb3 / ATG
                        3: 5065 .. 5073 / #a6acb3"
     CDS             join(5074..5076,5077..5079,5080..5783)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 5074 .. 5076 / #05fd14
                        2: 5077 .. 5079 / #05fd14 / 1a
                        3: 5080 .. 5783 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDE"
     primer_bind     5764..5823
                     /label=WPRE-F
                     /note="color: black; sequence: 
                     ACTCTCGGCATGGACGAGCTGTACAAGTGAaatcaacctctggattacaaaatttgtga
                     a"
     misc_feature    5794..6382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6370..6404)
                     /label=WPRE-R
                     /note="color: black; sequence: 
                     TATCGATGCGGCCGCTTTACTTgcggggaggcggc"
     primer_bind     complement(6400..6416)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             6926..7106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(7168..7756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(7927..8718,8719..8787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7927 .. 8718 / #ccffcc
                        2: 8719 .. 8787 / #ccffcc / signal sequence
                     Cleavage site after base 8718"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8788..8892)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
     2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3781 tcttccattt caggtgtcgt gagcggccgc tgagttaact attctagagc caccatggaa
     3841 actgacaccc tcttgctttg ggtgcttttg ttgtgggtac ccgggagtac gggtggtccg
     3901 gtccccccta gtacagcact tcgcgagctt atagaggaat tggtgaacat cacccagaac
     3961 caaaaggcgc ctctctgcaa cggttcaatg gtttggagta taaacttgac tgccggtatg
     4021 tattgcgcgg cgttggaatc actcatcaac gtatcaggtt gtagcgccat cgagaaaacg
     4081 caaagaatgc tttccggttt ctgtccccac aaggtaagcg cgggacaatt cagtagtttg
     4141 catgtacggg acactaaaat cgaggttgcc cagtttgtca aagaccttct gcttcatttg
     4201 aagaaactgt ttcgagaagg gcgattcaac tatccttacg atgtcccgga ctatgctgct
     4261 agcggcggag gtggaagcgg cgagttggcg gcgataaaac aagagcttgc agcaatcaaa
     4321 aaggagctgg cggcaattaa gtgggagctg gcggctatca agcaaggggc tggcaaggtg
     4381 gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc
     4441 atcatgcttt ggcagaagaa gccacgttga gaattctgag gatccgcccc tctccctccc
     4501 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4561 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4621 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4681 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4741 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4801 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4861 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4921 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat
     4981 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5041 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc
     5101 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5161 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5221 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     5281 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5341 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5401 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5461 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5521 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5581 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5641 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5701 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5761 atcactctcg gcatggacga gctgtacaag tgaaatcaac ctctggatta caaaatttgt
     5821 gaaagattga ctgatattct taactatgtt gctcctttta cgctgtgtgg atatgctgct
     5881 ttaatgcctc tgtatcatgc tattgcttcc cgtacggctt tcgttttctc ctccttgtat
     5941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtccgtca acgtggcgtg
     6001 gtgtgctctg tgtttgctga cgcaaccccc actggctggg gcattgccac cacctgtcaa
     6061 ctcctttctg ggactttcgc tttccccctc ccgatcgcca cggcagaact catcgccgcc
     6121 tgccttgccc gctgctggac aggggctagg ttgctgggca ctgataattc cgtggtgttg
     6181 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccaactg gatcctgcgc
     6241 gggacgtcct tctgctacgt cccttcggct ctcaatccag cggacctccc ttcccgaggc
     6301 cttctgccgg ttctgcggcc tctcccgcgt cttcgctttc ggcctccgac gagtcggatc
     6361 tccctttggg ccgcctcccc gcaagtaaag cggccgcatc gataccgtcg acctcgatcg
     6421 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     6481 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     6541 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6601 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6661 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6721 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6781 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6841 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6901 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7081 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     7141 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7921 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7981 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8041 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8101 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8161 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8221 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8281 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8341 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8401 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8461 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8521 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8581 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8641 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8701 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8761 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8821 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8881 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
