LOCUS       pHIV-IL13-IgG4hi        8899 bp    DNA     circular SYN 21-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        join(2624..3589,3590..3802)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="This forward directional feature has 2 segments:
                        1: 2624 .. 3589 / #ffffff
                        2: 3590 .. 3802 / #ffffff"
     intron          join(2855..3589,3590..3793)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="This feature has 2 segments:
                        1: 2855 .. 3589 / #------
                        2: 3590 .. 3793 / #------"
     primer_bind     complement(3424..3436)
                     /label=WPRE-R
                     /note="color: black; sequence: 
                     TATCGATGCGGCCGCTTTACTTgcggggaggcggc"
     primer_bind     3586..3612
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: GTTCTCGAGCTTTTGGAGTACGTCGTC"
     CDS             3835..3894
                     /codon_start=1
                     /label=IgK Leader
                     /note="color: #993366"
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3895..4230
                     /codon_start=1
                     /label=IL13
                     /note="color: #993366"
                     /translation="GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCA
                     ALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKK
                     LFREGRFN"
     CDS             4231..4257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             4258..4263
                     /label=Linker
                     /note="color: #993366; direction: RIGHT"
     CDS             4264..4299
                     /label=IgG4 hinge
                     /note="color: #666699; direction: RIGHT"
     primer_bind     complement(4285..4360)
                     /label=PDGFR_r1
                     /note="color: black; sequence: 
                     CCACCTTAAAGGGCAAGGAGTGTGGCACCACGATGACCTCCTGCGTGTCCTGGCCCACA
                     GCTGGGCACGGGGGGCA"
     CDS             4300..4446
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /note="color: #993366"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4335..4413)
                     /label=PDGFR_r2
                     /note="color: black; sequence: 
                     GATAAGGGAGATGATGGTGAGCACCACCAGGGCCAGGATGGCTGAGATCACCACCACCT
                     TAAAGGGCAAGGAGTGTGGC"
     primer_bind     complement(4386..4449)
                     /label=PDGFR_r3
                     /note="color: black; sequence: 
                     TCAACGTGGCTTCTTCTGCCAAAGCATGATGAGGATGATAAGGGAGATGATGGTGAGCA
                     CCACC"
     primer_bind     complement(4422..4486)
                     /label=pHIVW_PDGFR_gib_r
                     /note="color: black; sequence: 
                     GGGGGGGGGGAGGGAGAGGGGCGGATCCTCAGAATTCTCAACGTGGCTTCTTCTGCCAA
                     AGCATG"
     primer_bind     complement(4422..4466)
                     /label=pHIVW_gib2_r1
                     /note="color: black; sequence: 
                     GGGTACTCTAGATCAGCTAGCGAATCCTCCGAATTCTCAACGTGGCTTCTTCTGCCAAA
                     GCATG"
     misc_feature    join(4466..5040,5041..5043,5044..5052)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 4466 .. 5040 / #a6acb3
                        2: 5041 .. 5043 / #a6acb3 / ATG
                        3: 5044 .. 5052 / #a6acb3"
     CDS             join(5053..5055,5056..5058,5059..5762)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 5053 .. 5055 / #05fd14
                        2: 5056 .. 5058 / #05fd14 / 1a
                        3: 5059 .. 5762 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDE"
     primer_bind     5743..5802
                     /label=WPRE-F
                     /note="color: black; sequence: 
                     ACTCTCGGCATGGACGAGCTGTACAAGTGAaatcaacctctggattacaaaatttgtga
                     a"
     misc_feature    5773..6361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6349..6383)
                     /label=WPRE-R
                     /note="color: black; sequence: 
                     TATCGATGCGGCCGCTTTACTTgcggggaggcggc"
     primer_bind     complement(6379..6395)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             6905..7085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(7147..7735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(7906..8697,8698..8766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7906 .. 8697 / #ccffcc
                        2: 8698 .. 8766 / #ccffcc / signal sequence
                     Cleavage site after base 8697"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8767..8871)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
     2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3781 tcttccattt caggtgtcgt gagcggccgc tgagttaact attctagagc caccatggaa
     3841 actgacaccc tcttgctttg ggtgcttttg ttgtgggtac ccgggagtac gggtggtccg
     3901 gtccccccta gtacagcact tcgcgagctt atagaggaat tggtgaacat cacccagaac
     3961 caaaaggcgc ctctctgcaa cggttcaatg gtttggagta taaacttgac tgccggtatg
     4021 tattgcgcgg cgttggaatc actcatcaac gtatcaggtt gtagcgccat cgagaaaacg
     4081 caaagaatgc tttccggttt ctgtccccac aaggtaagcg cgggacaatt cagtagtttg
     4141 catgtacggg acactaaaat cgaggttgcc cagtttgtca aagaccttct gcttcatttg
     4201 aagaaactgt ttcgagaagg gcgattcaac tatccttacg atgtcccgga ctatgctgct
     4261 agcgaaagca agtatggtcc tccctgcccc ccgtgcccag ctgtgggcca ggacacgcag
     4321 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
     4381 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
     4441 ccacgttgag aattctgagg atccgcccct ctccctcccc cccccctaac gttactggcc
     4501 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4561 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4621 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4681 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4741 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4801 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4861 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4921 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa
     4981 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     5041 atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     5101 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     5161 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     5221 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     5281 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     5341 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     5401 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     5461 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     5521 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     5581 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5641 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     5701 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5761 ctgtacaagt gaaatcaacc tctggattac aaaatttgtg aaagattgac tgatattctt
     5821 aactatgttg ctccttttac gctgtgtgga tatgctgctt taatgcctct gtatcatgct
     5881 attgcttccc gtacggcttt cgttttctcc tccttgtata aatcctggtt gctgtctctt
     5941 tatgaggagt tgtggcccgt tgtccgtcaa cgtggcgtgg tgtgctctgt gtttgctgac
     6001 gcaaccccca ctggctgggg cattgccacc acctgtcaac tcctttctgg gactttcgct
     6061 ttccccctcc cgatcgccac ggcagaactc atcgccgcct gccttgcccg ctgctggaca
     6121 ggggctaggt tgctgggcac tgataattcc gtggtgttgt cggggaagct gacgtccttt
     6181 ccatggctgc tcgcctgtgt tgccaactgg atcctgcgcg ggacgtcctt ctgctacgtc
     6241 ccttcggctc tcaatccagc ggacctccct tcccgaggcc ttctgccggt tctgcggcct
     6301 ctcccgcgtc ttcgctttcg gcctccgacg agtcggatct ccctttgggc cgcctccccg
     6361 caagtaaagc ggccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     6421 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     6481 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     6541 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6601 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6661 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     6721 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     6781 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     6841 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     6901 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6961 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7021 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7081 tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8881 ccgaaaagtg ccacctgac
//
