LOCUS       Exported               15567 bp ds-DNA     circular SYN 20-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15567)
  AUTHORS   Dr John and Anne Chong Lab for Functional Genomics
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15567
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           668..751
                     /label=SFV 5'UTR
                     /note="color: #00ff00"
     misc_feature    752..8047
                     /label=nsP1-4
                     /note="color: #993366"
     CDS             752..2362
                     /codon_start=1
                     /label=nsP1
                     /note="color: #00ccff"
                     /translation="MAAKVHVDIEADSPFIKSLQKAFPSFEVESLQVTPNDHANARAFS
                     HLATKLIEQETDKDTLILDIGSAPSRRMMSTHKYHCVCPMRSAEDPERLVCYAKKLAAA
                     SEKVLDREIAGKITDLQTVMATPDAESPTFCLHTDVTCRTAAEVAVYQDVYAVHAPTSL
                     YHQAMKGVRTAYWIGFDTTPFMFDALAGAYPTYATNWADEQVLQARNIGLCAASLTEGR
                     LGKLSILRKKQLKPCDTVMFSVGSTLYTESRKLLRSWHLPSVFHLKGKQSFTCRCDTIV
                     SCEGYVVKKITMCPGLYGKTVGYAVTYHAEGFLVCKTTDTVKGERVSFPVCTYVPSTIC
                     DQMTGILATDITPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPIVAVAFSKWAREY
                     KADLDDEKPLGVRERSLTCCCLWAFKTRKMHTMYKKPDTQTIVKVPSEFNSFVIPSLWS
                     TGLAIPVRSRIKMLLAKKTKRESIPVLDASSARDAEQEEKERLEAELTREALPPLVPTA
                     PAETGVVDVDVEELEYHAGA"
     misc_feature    1067..1069
                     /label=G106E
                     /note="Middle G base converted to A, creates non-synonymous
                     G-->E. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1790..1792
                     /label=L347L
                     /note="Third position A base converted to G, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1802..1804
                     /label=V351I
                     /note="First position G base converted to A, non-synonymous
                     V-->I mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    2192..2194
                     /product="MUTAITON TO CHANGE L TO S AMINO ACID"
                     /label=L481S
                     /note="Second position T base converted to C, 
                     non-synonymous L-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2297..2299
                     /label=I516T
                     /note="Second position T base converted to C, 
                     non-synonymous I-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     CDS             2363..4759
                     /codon_start=1
                     /label=nsP2
                     /note="color: #ff99cc"
                     /translation="GVVETPRSALKVTAQPNGVLLGNYVVLSPQTVLKSSKLAPVHPLA
                     EQVKIITHNGRAGRYQVDGYDGRVLLPCGSAIPVPEFQALSESATMVYNEREFVNRKLY
                     HIAVHGPSLNTDEENYEKVRAERTDAEYVFDVDKKCCVKREEASGLVLVGELTNPPFHE
                     FAYEGLKIRPSAPYKTTVVGVFGVPGSGKSAIIKSLVTKHDLVTSGKKENCQEIVNDVK
                     KHRGLDIQAKTVDSILLNGCRRAVDILYVDEAFACHSGTLLALIALVKPRSKVVLCGDP
                     KQCGFFNMMQLKVNFNHNICTEVCHKSISRRCTRPVTAIVSTLHYGGKMRTTNPCNKPI
                     IIDTTGQTKPKPGDIVLTCFRGWVKQLQLDYRGHEVMTAAASQGLTRKGVYAVRQKVNE
                     NPLYAPASEHVNVLLTRTEDRLVWKTLAGDPWIKVLSNIPQGNFTATLEEWQEEHDKIM
                     KVIEGPAAPVDAFQNKANVCWAKSLVPVLDTAGIRLTAEEWSTIITAFKEDRAYSPVVA
                     LNEICTKYYGVDLDSGLFSAPKVSLYYENNHWDNRPGGRMYGFNAATAARLEARHTFLK
                     GQWHTGKQAVIAERKIQPLSVLDNVIPINRRLPHALVTEYKTVKGSRVEWLVNKVRGYH
                     VLLVSEYNLALPRRRVTWLSPLNVTGADRCYDLSLGLPRLEGRFDLVFVNIHTEFRIHH
                     YQQCVDHAMKLQMLGGDALRLLKPGGSLLMRAYGYADKISEAVVSSLSRKFSSARVLRP
                     DCVTSNTEVFLLFSNFDNGKRPSTLHQMNTKLSAVYAGEAMHTAGC"
     misc_feature    2414..2416
                     /label=D555G
                     /note="Second position A base converted to G, 
                     non-synonymous D-->G mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3149..3151
                     /label=L800L
                     /note="Third position A represents the synonymous mutation 
                     (G nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3329..3331
                     /label=T860T
                     /note="Third position G base converted to A, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3434..3490
                     /label=approximate packaging sequence
                     /note="PMID 9557722"
                     /note="color: #00ff00"
     misc_feature    4202..4204
                     /label=A1151T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    4382..4390
                     /product="Mutation made to reduce CPE (as described for 
                     Chikungunya virus in Akhrymuk, I., Lukash, T., Frolov, I. &
                     Frolova, E. I. Novel Mutations in nsP2 Abolish Chikungunya 
                     Virus-Induced Transcriptional Shutoff and Make the Virus 
                     Less Cytopathic without Affecting Its Replication Rates. J.
                     Virol. 93, (2019).)"
                     /label=ADA>RLE
                     /note="PMID: 31163352"
                     /note="color: #333399"
     CDS             4760..6205
                     /codon_start=1
                     /label=nsP3
                     /note="color: #ccffcc"
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGEATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDTRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERITRLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKVLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     misc_feature    5225..5227
                     /label=I1492I
                     /note="Third position A represents the synonymous mutation 
                     (T nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5231..5233
                     /product="MUTATE M TO T AMINO ACID"
                     /label=M1494T
                     /note="Second position T base converted to C, 
                     non-synonymous M-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5579..5581
                     /label=A1610T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    5983..6064
                     /label=CODON-OPTIMISED SEQUENCE FOR IDT SYNTHESIS
                     /note="This sequence contains the following 
                     codon-optimisations to reduce GC-content and make it 
                     synthesisable.g6217a, g6229a, g6235a, g6265t, g6283t, 
                     g6289t, g6298t"
                     /note="color: #333333"
     CDS             6206..8047
                     /codon_start=1
                     /label=nsP4
                     /note="color: #ffff99"
                     /translation="YIFSSDTGSGHLQQKSVRQHNLQCAQLDAVEEEKMYPPKLDTERE
                     KLLLLKMQMHPSEANKSRYQSRKVENMKATVVDRLTSGARLYTGADVGRIPTYAVRYPR
                     PVYSPTVIERFSSPDVAIAACNEYLSRNYPTVASYQITDEYDAYLDMVDGSDSCLDRAT
                     FCPAKLRCYPKHHAYHQPTVRSAVPSPFQNTLQSVLAAATKRNCNVTQMRELPTMDSAV
                     FNVECFKRYACSGEYWEEYAKQPIRITTENITTYVTKLKGPKAAALFAKTHNLVPLQEV
                     PMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLRP
                     NVHTLFDMSAEDFDAIIASHFHPGDPVLETDIASFDKSQDDSLALTGLMILEDLGVDQY
                     LLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFINTVLNITIASRVLEQRLTDS
                     ACAAFIGDDNIVHGVISDKLMAERCASWVNMEVKIIDAVMGEKPPYFCGGFIVFDSVTQ
                     TACRVSDPLKRLFKLGKPLTAEDKQDEDRRRALSDEVSKWFRTGLGAELEVALTSRYKV
                     EGCKSILIAMATLARDIKAFKKLRGPVIHLYGGPRLVR"
     misc_feature    6794..6796
                     /label=N2015S
                     /note="Second position A base converted to G, 
                     non-synonymous N-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    7928..7930
                     /label=E2393K
                     /note="First position G base converted to A, non-synonymous
                     E-->K mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    8051..8088
                     /label=SGP
                     /note="color: #666699"
     CDS             8089..8805
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1: 8089 .. 8091 / #05fc14
                        2: 8092 .. 8094 / #05fc14 / 1a
                        3: 8095 .. 8805 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8818..8874
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 8871"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8875..10533
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /note="color: #ffcc00"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAVSTV"
     3'UTR           10537..10797
                     /label=SFV Viral 3'UTR
                     /note="color: #ff6600"
     polyA_site      10798..10867
                     /label=PolyA Tail
                     /note="color: #------"
     polyA_signal    10933..11157
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      11203..11631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        11645..11974
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      11825..11960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             12041..12835
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    13009..13130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(13179..13195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    13203..13219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(13227..13257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 13227 .. 13233 / #ffffff / -10
                        2: 13234 .. 13251 / #ffffff
                        3: 13252 .. 13257 / #ffffff / -35"
     protein_bind    13272..13293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(13581..14166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(14337..15197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 14337 .. 15128 / #ccffcc
                        2: 15129 .. 15197 / #ccffcc / signal sequence
                     Cleavage site after base 15128"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15198..15302)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgg cggatgtgtg acatacacga cgccaaaaga ttttgttcca gctcctgcca
      721 cctccgctac gcgagagatt aaccacccac gatggccgcc aaagtgcatg ttgatattga
      781 ggctgacagc ccattcatca agtctttgca gaaggcattt ccgtcgttcg aggtggagtc
      841 attgcaggtc acaccaaatg accatgcaaa tgccagagca ttttcgcacc tggctaccaa
      901 attgatcgag caggagactg acaaagacac actcatcttg gatatcggca gtgcgccttc
      961 caggagaatg atgtctacgc acaaatacca ctgcgtatgc cctatgcgca gcgcagaaga
     1021 ccccgaaagg ctcgtatgct acgcaaagaa actggcagcg gcctccgaga aggtgctgga
     1081 tagagagatc gcaggaaaaa tcaccgacct gcagaccgtc atggctacgc cagacgctga
     1141 atctcctacc ttttgcctgc atacagacgt cacgtgtcgt acggcagccg aagtggccgt
     1201 ataccaggac gtgtatgctg tacatgcacc aacatcgctg taccatcagg cgatgaaagg
     1261 tgtcagaacg gcgtattgga ttgggtttga caccaccccg tttatgtttg acgcgctagc
     1321 aggcgcgtat ccaacctacg ccacaaactg ggccgacgag caggtgttac aggccaggaa
     1381 cataggactg tgtgcagcat ccttgactga gggaagactc ggcaaactgt ccattctccg
     1441 caagaagcaa ttgaaacctt gcgacacagt catgttctcg gtaggatcta cattgtacac
     1501 tgagagcaga aagctactga ggagctggca cttaccctcc gtattccacc tgaaaggtaa
     1561 acaatccttt acctgtaggt gcgataccat cgtatcatgt gaagggtacg tagttaagaa
     1621 aatcactatg tgccccggcc tgtacggtaa aacggtaggg tacgccgtga cgtatcacgc
     1681 ggagggattc ctagtgtgca agaccacaga cactgtcaaa ggagaaagag tctcattccc
     1741 tgtatgcacc tacgtcccct caaccatctg tgatcaaatg actggcatac tggcgaccga
     1801 catcacaccg gaggacgcac agaagttgtt agtgggattg aatcagagga tagttgtgaa
     1861 cggaagaaca cagcgaaaca ctaacacgat gaagaactat ctgcttccga ttgtggccgt
     1921 cgcatttagc aagtgggcga gggaatacaa ggcagacctt gatgatgaaa aacctctggg
     1981 tgtccgagag aggtcactta cttgctgctg cttgtgggca tttaaaacga ggaagatgca
     2041 caccatgtac aagaaaccag acacccagac aatagtgaag gtgccttcag agtttaactc
     2101 gttcgtcatc ccgagcctat ggtctacagg cctcgcaatc ccagtcagat cacgcattaa
     2161 gatgcttttg gccaagaaga ccaagcgaga gtcaatacct gttctcgacg cgtcgtcagc
     2221 cagggatgct gaacaagagg agaaggagag gttggaggcc gagctgacta gagaagcctt
     2281 accacccctc gtccccaccg cgccggcgga gacgggagtc gtcgacgtcg acgttgaaga
     2341 actagagtat cacgcaggtg caggggtcgt ggaaacacct cgcagcgcgt tgaaagtcac
     2401 cgcacagccg aacggcgtac tactaggaaa ttacgtagtt ctgtccccgc agaccgtgct
     2461 caagagctcc aagttggccc ccgtgcaccc tctagcagag caggtgaaaa taataacaca
     2521 taacgggagg gccggccgtt accaggtcga cggatatgac ggcagggtcc tactaccatg
     2581 tggatcggcc attccggtcc ctgagtttca agctttgagc gagagcgcca ctatggtgta
     2641 caacgaaagg gagttcgtca acaggaaact ataccatatt gccgttcacg gaccgtcgct
     2701 gaacaccgac gaggagaact acgagaaagt cagagctgaa agaactgacg ccgagtacgt
     2761 gttcgacgta gataaaaaat gctgcgtcaa gagagaggaa gcgtcgggtt tggtgttggt
     2821 gggagagcta accaaccccc cgttccatga attcgcctac gaagggctga agatcaggcc
     2881 gtcggcacca tataagacta cagtagtagg agtctttggg gttccgggat caggcaagtc
     2941 tgctattatt aagagcctcg tgaccaaaca cgatctggtc accagcggca agaaggagaa
     3001 ctgccaggaa atagtcaacg acgtgaagaa gcaccgcgga ctggacatcc aggcaaaaac
     3061 agtggactcc atcctgctaa acgggtgtcg tcgtgccgtg gacatcctat atgtggacga
     3121 ggctttcgct tgccattccg gtactctgct agccctaatt gctcttgtta aacctcggag
     3181 caaagtggtg ttatgcggag accccaagca atgcggattc ttcaatatga tgcagcttaa
     3241 ggtgaacttc aaccacaaca tctgcactga agtatgtcat aaaagtatat ccagacgttg
     3301 cacgcgtcca gtcacggcca tcgtgtctac attgcactac ggaggcaaga tgcgcacgac
     3361 caacccgtgc aacaaaccca taatcataga caccacagga cagaccaagc ccaagccagg
     3421 agacatcgtg ttaacatgct tccgaggctg ggtaaagcag ctgcagttgg actaccgtgg
     3481 acacgaagtc atgacagcag cagcatctca gggcctcacc cgcaaagggg tatacgccgt
     3541 aaggcagaag gtgaatgaaa atcccttgta tgcccctgcg tcggagcacg tgaatgtact
     3601 gctgacgcgc actgaggata ggctggtgtg gaaaacgctg gccggcgatc cctggattaa
     3661 ggtcctatca aacattccac agggtaactt tacggccaca ttggaagaat ggcaagaaga
     3721 acacgacaaa ataatgaagg tgattgaagg accggctgcg cctgtggacg cgttccagaa
     3781 caaagcgaac gtgtgttggg cgaaaagcct ggtgcctgtc ctggacactg ccggaatcag
     3841 attgacagca gaggagtgga gcaccataat tacagcattt aaggaggaca gagcttactc
     3901 tccagtggtg gccttgaatg aaatttgcac caagtactat ggagttgacc tggacagtgg
     3961 cctgttttct gccccgaagg tgtccctgta ttacgagaac aaccactggg ataacagacc
     4021 tggtggaagg atgtatggat tcaatgccgc aacagctgcc aggctggaag ctagacatac
     4081 cttcctgaag gggcagtggc atacgggcaa gcaggcagtt atcgcagaaa gaaaaatcca
     4141 accgctttct gtgctggaca atgtaattcc tatcaaccgc aggctgccgc acgccctggt
     4201 gactgagtac aagacggtta aaggcagtag ggttgagtgg ctggtcaata aagtaagagg
     4261 gtaccacgtc ctgctggtga gtgagtacaa cctggctttg cctcgacgca gggtcacttg
     4321 gttgtcaccg ctgaatgtca caggcgccga taggtgctac gacctaagtt taggactgcc
     4381 gcggctggaa ggcaggttcg acttggtctt tgtgaacatt cacacggaat tcagaatcca
     4441 ccactaccag cagtgtgtcg accacgccat gaagctgcag atgcttgggg gagatgcgct
     4501 acgactgcta aaacccggcg gcagcctctt gatgagagct tacggatacg ccgataaaat
     4561 cagcgaagcc gttgtttcct ccttaagcag aaagttctcg tctgcaagag tgttgcgccc
     4621 ggattgtgtc accagcaata cagaagtgtt cttgctgttc tccaactttg acaacggaaa
     4681 gagaccctct acgctacacc agatgaatac caagctgagt gccgtgtatg ccggagaagc
     4741 catgcacacg gccgggtgtg caccatccta cagagttaag agagcagaca tagccacgtg
     4801 cacagaagcg gctgtggtta acgcagctaa cgcccgtgga actgtagggg atggcgtatg
     4861 cagggccgtg gcgaagaaat ggccgtcagc ctttaaggga gaagcaacac cagtgggcac
     4921 aattaaaaca gtcatgtgcg gctcgtaccc cgtcatccac gctgtagcgc ctaatttctc
     4981 tgccacgact gaagcggaag gggaccgcga attggccgct gtctaccggg cagtggccgc
     5041 cgaagtaaac agactgtcac tgagcagcgt agccatcccg ctgctgtcca caggagtgtt
     5101 cagcggcgga agagataggc tgcagcaatc cctcaaccat ctattcacag caatggacgc
     5161 cacggacgct gacgtgacca tctactgcag agacaaaagt tgggagaaga aaatccagga
     5221 agccatagac acgaggacgg ctgtggagtt gctcaatgat gacgtggagc tgaccacaga
     5281 cttggtgaga gtgcacccgg acagcagcct ggtgggtcgt aagggctaca gtaccactga
     5341 cgggtcgctg tactcgtact ttgaaggtac gaaattcaac caggctgcta ttgatatggc
     5401 agagatactg acgttgtggc ccagactgca agaggcaaac gaacagatat gcctatacgc
     5461 gctgggcgaa acaatggaca acatcagatc caaatgtccg gtgaacgatt ccgattcatc
     5521 aacacctccc aggacagtgc cctgcctgtg ccgctacgca atgacagcag aacggatcac
     5581 ccgccttagg tcacaccaag ttaaaagcat ggtggtttgc tcatcttttc ccctcccgaa
     5641 ataccatgta gatggggtgc agaaggtaaa gtgcgagaag gttctcctgt tcgacccgac
     5701 ggtaccttca gtggttagtc cgcggaagta tgccgcatct acgacggacc actcagatcg
     5761 gtcgttacga gggtttgact tggactggac caccgactcg tcttccactg ccagcgatac
     5821 catgtcgcta cccagtttgc agtcgtgtga catcgactcg atctacgagc caatggctcc
     5881 catagtagtg acggctgacg tacaccctga acccgcaggc atcgcggacc tggcggcaga
     5941 tgtgcatcct gaacccgcag accatgtgga cctcgagaac ccaattcctc caccacgccc
     6001 aaagagagct gcataccttg cctcccgcgc tgcggagcga ccggtgcctg cgcctagaaa
     6061 gcctacgcct gccccaagga ctgcgtttag gaacaagctg cctttgacgt tcggcgactt
     6121 tgacgagcac gaggtcgatg cgttggcctc cgggattact ttcggagact tcgacgacgt
     6181 cctgcgacta ggccgcgcgg gtgcatatat tttctcctcg gacactggca gcggacattt
     6241 acaacaaaaa tccgttaggc agcacaatct ccagtgcgca caactggatg cggtcgagga
     6301 ggagaaaatg tacccgccaa aattggatac tgagagggag aagctgttgc tgctgaaaat
     6361 gcagatgcac ccatcggagg ctaataagag tcgataccag tctcgcaaag tggagaacat
     6421 gaaagccacg gtggtggaca ggctcacatc gggggccaga ttgtacacgg gagcggacgt
     6481 aggccgcata ccaacatacg cggttcggta cccccgcccc gtgtactccc ctaccgtgat
     6541 cgaaagattc tcaagccccg atgtagcaat cgcagcgtgc aacgaatacc tatccagaaa
     6601 ttacccaaca gtggcgtcgt accagataac agatgaatac gacgcatact tggacatggt
     6661 tgacgggtcg gatagttgct tggacagagc gacattctgc ccggcgaagc tccggtgcta
     6721 cccgaaacat catgcgtacc accagccgac tgtacgcagt gccgtcccgt caccctttca
     6781 gaacacacta cagagcgtgc tagcggccgc caccaagaga aactgcaacg tcacgcaaat
     6841 gcgagaacta cccaccatgg actcggcagt gttcaacgtg gagtgcttca agcgctatgc
     6901 ctgctccgga gaatattggg aagaatatgc taaacaacct atccggataa ccactgagaa
     6961 catcactacc tatgtgacca aattgaaagg cccgaaagct gctgccttgt tcgctaagac
     7021 ccacaacttg gttccgctgc aggaggttcc catggacaga ttcacggtcg acatgaaacg
     7081 agatgtcaaa gtcactccag ggacgaaaca cacagaggaa agacccaaag tccaggtaat
     7141 tcaagcagcg gagccattgg cgaccgctta cctgtgcggc atccacaggg aattagtaag
     7201 gagactaaat gctgtgttac gccctaacgt gcacacattg tttgatatgt cggccgaaga
     7261 ctttgacgcg atcatcgcct ctcacttcca cccaggagac ccggttctag agacggacat
     7321 tgcatcattc gacaaaagcc aggacgactc cttggctctt acaggtttaa tgatcctcga
     7381 agatctaggg gtggatcagt acctgctgga cttgatcgag gcagcctttg gggaaatatc
     7441 cagctgtcac ctaccaactg gcacgcgctt caagttcgga gctatgatga aatcgggcat
     7501 gtttctgact ttgtttatta acactgtttt gaacatcacc atagcaagca gggtactgga
     7561 gcagagactc actgactccg cctgtgcggc cttcatcggc gacgacaaca tcgttcacgg
     7621 agtgatctcc gacaagctga tggcggagag gtgcgcgtcg tgggtcaaca tggaggtgaa
     7681 gatcattgac gctgtcatgg gcgaaaaacc cccatatttt tgtgggggat tcatagtttt
     7741 tgacagcgtc acacagaccg cctgccgtgt ttcagaccca cttaagcgcc tgttcaagtt
     7801 gggtaagccg ctaacagctg aagacaagca ggacgaagac aggcgacgag cactgagtga
     7861 cgaggttagc aagtggttcc ggacaggctt gggggccgaa ctggaggtgg cactaacatc
     7921 taggtataag gtagagggct gcaaaagtat cctcatagcc atggccacct tggcgaggga
     7981 cattaaggcg tttaagaaat tgagaggacc tgttatacac ctctacggcg gtcctagatt
     8041 ggtgcgttaa tacacagaat tctgattata gcgcactatt atagcaccat ggtgagcaag
     8101 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     8161 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     8221 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     8281 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     8341 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     8401 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     8461 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     8521 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     8581 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8641 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     8701 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8761 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtcagg atccggagcc
     8821 acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg gcccatggaa
     8881 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
     8941 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
     9001 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
     9061 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
     9121 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
     9181 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
     9241 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
     9301 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
     9361 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
     9421 cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc
     9481 ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc
     9541 cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca
     9601 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
     9661 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
     9721 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
     9781 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
     9841 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
     9901 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
     9961 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg
    10021 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
    10081 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
    10141 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
    10201 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
    10261 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
    10321 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
    10381 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
    10441 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
    10501 aagaagggcg gcaagatcgc cgtgtcgact gtttaagtta gggtaggcaa tggcattgat
    10561 atagcaagaa aattgaaaac agaaaaagtt agggtaagca atggcatata accataactg
    10621 tataacttgt aacaaagcgc aacaagacct gcgcaattgg ccccgtggtc cgcctcacgg
    10681 aaactcgggg caactcatat tgacacatta attggcaata attggaagct tacataagct
    10741 taattcgacg aataattgga tttttatttt attttgcaat tggtttttaa tatttccaaa
    10801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
    10861 aaaaaaacta gtctgcatta atgaatcggc tcgagtctag agggcccgtt taaacccgct
    10921 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    10981 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    11041 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    11101 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    11161 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    11221 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    11281 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    11341 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    11401 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    11461 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    11521 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    11581 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    11641 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11701 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    11761 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    11821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    11881 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    11941 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    12001 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
    12061 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
    12121 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
    12181 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
    12241 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    12301 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    12361 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
    12421 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
    12481 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
    12541 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
    12601 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
    12661 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
    12721 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
    12781 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
    12841 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
    12901 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    12961 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    13021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    13081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    13141 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    13201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    13261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    13321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    13381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    13441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    13501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    13561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    13621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    13681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    13741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    13801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    13861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    13921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    13981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    14041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    14101 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    14161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    14221 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    14281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    14341 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    14401 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    14461 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    14521 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    14581 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    14641 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    14701 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    14761 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    14821 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    14881 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    14941 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    15001 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    15061 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    15121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    15181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    15241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    15301 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    15361 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    15421 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    15481 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    15541 gcgatgtacg ggccagatat acgcgtt
//
