LOCUS       Exported               13866 bp ds-DNA     circular SYN 20-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Dr John and Anne Chong Lab for Functional Genomics
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           668..751
                     /label=SFV 5'UTR
                     /note="color: #00ff00"
     misc_feature    752..8047
                     /label=nsP1-4
                     /note="color: #993366"
     CDS             752..2362
                     /codon_start=1
                     /label=nsP1
                     /note="color: #00ccff"
                     /translation="MAAKVHVDIEADSPFIKSLQKAFPSFEVESLQVTPNDHANARAFS
                     HLATKLIEQETDKDTLILDIGSAPSRRMMSTHKYHCVCPMRSAEDPERLVCYAKKLAAA
                     SEKVLDREIAGKITDLQTVMATPDAESPTFCLHTDVTCRTAAEVAVYQDVYAVHAPTSL
                     YHQAMKGVRTAYWIGFDTTPFMFDALAGAYPTYATNWADEQVLQARNIGLCAASLTEGR
                     LGKLSILRKKQLKPCDTVMFSVGSTLYTESRKLLRSWHLPSVFHLKGKQSFTCRCDTIV
                     SCEGYVVKKITMCPGLYGKTVGYAVTYHAEGFLVCKTTDTVKGERVSFPVCTYVPSTIC
                     DQMTGILATDITPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPIVAVAFSKWAREY
                     KADLDDEKPLGVRERSLTCCCLWAFKTRKMHTMYKKPDTQTIVKVPSEFNSFVIPSLWS
                     TGLAIPVRSRIKMLLAKKTKRESIPVLDASSARDAEQEEKERLEAELTREALPPLVPTA
                     PAETGVVDVDVEELEYHAGA"
     misc_feature    1067..1069
                     /label=G106E
                     /note="Middle G base converted to A, creates non-synonymous
                     G-->E. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1790..1792
                     /label=L347L
                     /note="Third position A base converted to G, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1802..1804
                     /label=V351I
                     /note="First position G base converted to A, non-synonymous
                     V-->I mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    2192..2194
                     /product="MUTAITON TO CHANGE L TO S AMINO ACID"
                     /label=L481S
                     /note="Second position T base converted to C, 
                     non-synonymous L-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2297..2299
                     /label=I516T
                     /note="Second position T base converted to C, 
                     non-synonymous I-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     CDS             2363..4759
                     /codon_start=1
                     /label=nsP2
                     /note="color: #ff99cc"
                     /translation="GVVETPRSALKVTAQPNGVLLGNYVVLSPQTVLKSSKLAPVHPLA
                     EQVKIITHNGRAGRYQVDGYDGRVLLPCGSAIPVPEFQALSESATMVYNEREFVNRKLY
                     HIAVHGPSLNTDEENYEKVRAERTDAEYVFDVDKKCCVKREEASGLVLVGELTNPPFHE
                     FAYEGLKIRPSAPYKTTVVGVFGVPGSGKSAIIKSLVTKHDLVTSGKKENCQEIVNDVK
                     KHRGLDIQAKTVDSILLNGCRRAVDILYVDEAFACHSGTLLALIALVKPRSKVVLCGDP
                     KQCGFFNMMQLKVNFNHNICTEVCHKSISRRCTRPVTAIVSTLHYGGKMRTTNPCNKPI
                     IIDTTGQTKPKPGDIVLTCFRGWVKQLQLDYRGHEVMTAAASQGLTRKGVYAVRQKVNE
                     NPLYAPASEHVNVLLTRTEDRLVWKTLAGDPWIKVLSNIPQGNFTATLEEWQEEHDKIM
                     KVIEGPAAPVDAFQNKANVCWAKSLVPVLDTAGIRLTAEEWSTIITAFKEDRAYSPVVA
                     LNEICTKYYGVDLDSGLFSAPKVSLYYENNHWDNRPGGRMYGFNAATAARLEARHTFLK
                     GQWHTGKQAVIAERKIQPLSVLDNVIPINRRLPHALVTEYKTVKGSRVEWLVNKVRGYH
                     VLLVSEYNLALPRRRVTWLSPLNVTGADRCYDLSLGLPRLEGRFDLVFVNIHTEFRIHH
                     YQQCVDHAMKLQMLGGDALRLLKPGGSLLMRAYGYADKISEAVVSSLSRKFSSARVLRP
                     DCVTSNTEVFLLFSNFDNGKRPSTLHQMNTKLSAVYAGEAMHTAGC"
     misc_feature    2414..2416
                     /label=D555G
                     /note="Second position A base converted to G, 
                     non-synonymous D-->G mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3149..3151
                     /label=L800L
                     /note="Third position A represents the synonymous mutation 
                     (G nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3329..3331
                     /label=T860T
                     /note="Third position G base converted to A, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3434..3490
                     /label=approximate packaging sequence
                     /note="PMID 9557722"
                     /note="color: #00ff00"
     misc_feature    4202..4204
                     /label=A1151T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    4382..4390
                     /label=ADA>RLE
                     /note="PMID: 31163352"
                     /note="color: #666699"
     CDS             4760..6205
                     /codon_start=1
                     /label=nsP3
                     /note="color: #ccffcc"
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGEATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDTRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERITRLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKVLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     misc_feature    5225..5227
                     /label=I1492I
                     /note="Third position A represents the synonymous mutation 
                     (T nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5231..5233
                     /product="MUTATE M TO T AMINO ACID"
                     /label=M1494T
                     /note="Second position T base converted to C, 
                     non-synonymous M-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5579..5581
                     /label=A1610T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    5983..6064
                     /label=CODON-OPTIMISED SEQUENCE FOR IDT SYNTHESIS
                     /note="This sequence contains the following 
                     codon-optimisations to reduce GC-content and make it 
                     synthesisable.g6217a, g6229a, g6235a, g6265t, g6283t, 
                     g6289t, g6298t"
                     /note="color: #333333"
     CDS             6206..8047
                     /codon_start=1
                     /label=nsP4
                     /note="color: #ffff99"
                     /translation="YIFSSDTGSGHLQQKSVRQHNLQCAQLDAVEEEKMYPPKLDTERE
                     KLLLLKMQMHPSEANKSRYQSRKVENMKATVVDRLTSGARLYTGADVGRIPTYAVRYPR
                     PVYSPTVIERFSSPDVAIAACNEYLSRNYPTVASYQITDEYDAYLDMVDGSDSCLDRAT
                     FCPAKLRCYPKHHAYHQPTVRSAVPSPFQNTLQSVLAAATKRNCNVTQMRELPTMDSAV
                     FNVECFKRYACSGEYWEEYAKQPIRITTENITTYVTKLKGPKAAALFAKTHNLVPLQEV
                     PMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLRP
                     NVHTLFDMSAEDFDAIIASHFHPGDPVLETDIASFDKSQDDSLALTGLMILEDLGVDQY
                     LLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFINTVLNITIASRVLEQRLTDS
                     ACAAFIGDDNIVHGVISDKLMAERCASWVNMEVKIIDAVMGEKPPYFCGGFIVFDSVTQ
                     TACRVSDPLKRLFKLGKPLTAEDKQDEDRRRALSDEVSKWFRTGLGAELEVALTSRYKV
                     EGCKSILIAMATLARDIKAFKKLRGPVIHLYGGPRLVR"
     misc_feature    6794..6796
                     /label=N2015S
                     /note="Second position A base converted to G, 
                     non-synonymous N-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    7928..7930
                     /label=E2393K
                     /note="First position G base converted to A, non-synonymous
                     E-->K mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    8051..8088
                     /label=SGP
                     /note="color: #666699"
     CDS             8089..8832
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="This feature has 2 segments:
                        1: 8089 .. 8460 / #008080 / rtTA
                        2: 8461 .. 8832 / #999999 / VP16"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     3'UTR           8836..9096
                     /label=SFV Viral 3'UTR
                     /note="color: #ff6600"
     polyA_site      9097..9166
                     /label=PolyA Tail
                     /note="color: #------"
     polyA_signal    9232..9456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      9502..9930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        9944..10273
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10124..10259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             10340..11134
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    11308..11429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(11478..11494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    11502..11518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(11526..11556)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 11526 .. 11532 / #ffffff / -10
                        2: 11533 .. 11550 / #ffffff
                        3: 11551 .. 11556 / #ffffff / -35"
     protein_bind    11571..11592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(11880..12465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12636..13496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 12636 .. 13427 / #ccffcc
                        2: 13428 .. 13496 / #ccffcc / signal sequence
                     Cleavage site after base 13427"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13497..13601)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgg cggatgtgtg acatacacga cgccaaaaga ttttgttcca gctcctgcca
      721 cctccgctac gcgagagatt aaccacccac gatggccgcc aaagtgcatg ttgatattga
      781 ggctgacagc ccattcatca agtctttgca gaaggcattt ccgtcgttcg aggtggagtc
      841 attgcaggtc acaccaaatg accatgcaaa tgccagagca ttttcgcacc tggctaccaa
      901 attgatcgag caggagactg acaaagacac actcatcttg gatatcggca gtgcgccttc
      961 caggagaatg atgtctacgc acaaatacca ctgcgtatgc cctatgcgca gcgcagaaga
     1021 ccccgaaagg ctcgtatgct acgcaaagaa actggcagcg gcctccgaga aggtgctgga
     1081 tagagagatc gcaggaaaaa tcaccgacct gcagaccgtc atggctacgc cagacgctga
     1141 atctcctacc ttttgcctgc atacagacgt cacgtgtcgt acggcagccg aagtggccgt
     1201 ataccaggac gtgtatgctg tacatgcacc aacatcgctg taccatcagg cgatgaaagg
     1261 tgtcagaacg gcgtattgga ttgggtttga caccaccccg tttatgtttg acgcgctagc
     1321 aggcgcgtat ccaacctacg ccacaaactg ggccgacgag caggtgttac aggccaggaa
     1381 cataggactg tgtgcagcat ccttgactga gggaagactc ggcaaactgt ccattctccg
     1441 caagaagcaa ttgaaacctt gcgacacagt catgttctcg gtaggatcta cattgtacac
     1501 tgagagcaga aagctactga ggagctggca cttaccctcc gtattccacc tgaaaggtaa
     1561 acaatccttt acctgtaggt gcgataccat cgtatcatgt gaagggtacg tagttaagaa
     1621 aatcactatg tgccccggcc tgtacggtaa aacggtaggg tacgccgtga cgtatcacgc
     1681 ggagggattc ctagtgtgca agaccacaga cactgtcaaa ggagaaagag tctcattccc
     1741 tgtatgcacc tacgtcccct caaccatctg tgatcaaatg actggcatac tggcgaccga
     1801 catcacaccg gaggacgcac agaagttgtt agtgggattg aatcagagga tagttgtgaa
     1861 cggaagaaca cagcgaaaca ctaacacgat gaagaactat ctgcttccga ttgtggccgt
     1921 cgcatttagc aagtgggcga gggaatacaa ggcagacctt gatgatgaaa aacctctggg
     1981 tgtccgagag aggtcactta cttgctgctg cttgtgggca tttaaaacga ggaagatgca
     2041 caccatgtac aagaaaccag acacccagac aatagtgaag gtgccttcag agtttaactc
     2101 gttcgtcatc ccgagcctat ggtctacagg cctcgcaatc ccagtcagat cacgcattaa
     2161 gatgcttttg gccaagaaga ccaagcgaga gtcaatacct gttctcgacg cgtcgtcagc
     2221 cagggatgct gaacaagagg agaaggagag gttggaggcc gagctgacta gagaagcctt
     2281 accacccctc gtccccaccg cgccggcgga gacgggagtc gtcgacgtcg acgttgaaga
     2341 actagagtat cacgcaggtg caggggtcgt ggaaacacct cgcagcgcgt tgaaagtcac
     2401 cgcacagccg aacggcgtac tactaggaaa ttacgtagtt ctgtccccgc agaccgtgct
     2461 caagagctcc aagttggccc ccgtgcaccc tctagcagag caggtgaaaa taataacaca
     2521 taacgggagg gccggccgtt accaggtcga cggatatgac ggcagggtcc tactaccatg
     2581 tggatcggcc attccggtcc ctgagtttca agctttgagc gagagcgcca ctatggtgta
     2641 caacgaaagg gagttcgtca acaggaaact ataccatatt gccgttcacg gaccgtcgct
     2701 gaacaccgac gaggagaact acgagaaagt cagagctgaa agaactgacg ccgagtacgt
     2761 gttcgacgta gataaaaaat gctgcgtcaa gagagaggaa gcgtcgggtt tggtgttggt
     2821 gggagagcta accaaccccc cgttccatga attcgcctac gaagggctga agatcaggcc
     2881 gtcggcacca tataagacta cagtagtagg agtctttggg gttccgggat caggcaagtc
     2941 tgctattatt aagagcctcg tgaccaaaca cgatctggtc accagcggca agaaggagaa
     3001 ctgccaggaa atagtcaacg acgtgaagaa gcaccgcgga ctggacatcc aggcaaaaac
     3061 agtggactcc atcctgctaa acgggtgtcg tcgtgccgtg gacatcctat atgtggacga
     3121 ggctttcgct tgccattccg gtactctgct agccctaatt gctcttgtta aacctcggag
     3181 caaagtggtg ttatgcggag accccaagca atgcggattc ttcaatatga tgcagcttaa
     3241 ggtgaacttc aaccacaaca tctgcactga agtatgtcat aaaagtatat ccagacgttg
     3301 cacgcgtcca gtcacggcca tcgtgtctac attgcactac ggaggcaaga tgcgcacgac
     3361 caacccgtgc aacaaaccca taatcataga caccacagga cagaccaagc ccaagccagg
     3421 agacatcgtg ttaacatgct tccgaggctg ggtaaagcag ctgcagttgg actaccgtgg
     3481 acacgaagtc atgacagcag cagcatctca gggcctcacc cgcaaagggg tatacgccgt
     3541 aaggcagaag gtgaatgaaa atcccttgta tgcccctgcg tcggagcacg tgaatgtact
     3601 gctgacgcgc actgaggata ggctggtgtg gaaaacgctg gccggcgatc cctggattaa
     3661 ggtcctatca aacattccac agggtaactt tacggccaca ttggaagaat ggcaagaaga
     3721 acacgacaaa ataatgaagg tgattgaagg accggctgcg cctgtggacg cgttccagaa
     3781 caaagcgaac gtgtgttggg cgaaaagcct ggtgcctgtc ctggacactg ccggaatcag
     3841 attgacagca gaggagtgga gcaccataat tacagcattt aaggaggaca gagcttactc
     3901 tccagtggtg gccttgaatg aaatttgcac caagtactat ggagttgacc tggacagtgg
     3961 cctgttttct gccccgaagg tgtccctgta ttacgagaac aaccactggg ataacagacc
     4021 tggtggaagg atgtatggat tcaatgccgc aacagctgcc aggctggaag ctagacatac
     4081 cttcctgaag gggcagtggc atacgggcaa gcaggcagtt atcgcagaaa gaaaaatcca
     4141 accgctttct gtgctggaca atgtaattcc tatcaaccgc aggctgccgc acgccctggt
     4201 gactgagtac aagacggtta aaggcagtag ggttgagtgg ctggtcaata aagtaagagg
     4261 gtaccacgtc ctgctggtga gtgagtacaa cctggctttg cctcgacgca gggtcacttg
     4321 gttgtcaccg ctgaatgtca caggcgccga taggtgctac gacctaagtt taggactgcc
     4381 gcggctggaa ggcaggttcg acttggtctt tgtgaacatt cacacggaat tcagaatcca
     4441 ccactaccag cagtgtgtcg accacgccat gaagctgcag atgcttgggg gagatgcgct
     4501 acgactgcta aaacccggcg gcagcctctt gatgagagct tacggatacg ccgataaaat
     4561 cagcgaagcc gttgtttcct ccttaagcag aaagttctcg tctgcaagag tgttgcgccc
     4621 ggattgtgtc accagcaata cagaagtgtt cttgctgttc tccaactttg acaacggaaa
     4681 gagaccctct acgctacacc agatgaatac caagctgagt gccgtgtatg ccggagaagc
     4741 catgcacacg gccgggtgtg caccatccta cagagttaag agagcagaca tagccacgtg
     4801 cacagaagcg gctgtggtta acgcagctaa cgcccgtgga actgtagggg atggcgtatg
     4861 cagggccgtg gcgaagaaat ggccgtcagc ctttaaggga gaagcaacac cagtgggcac
     4921 aattaaaaca gtcatgtgcg gctcgtaccc cgtcatccac gctgtagcgc ctaatttctc
     4981 tgccacgact gaagcggaag gggaccgcga attggccgct gtctaccggg cagtggccgc
     5041 cgaagtaaac agactgtcac tgagcagcgt agccatcccg ctgctgtcca caggagtgtt
     5101 cagcggcgga agagataggc tgcagcaatc cctcaaccat ctattcacag caatggacgc
     5161 cacggacgct gacgtgacca tctactgcag agacaaaagt tgggagaaga aaatccagga
     5221 agccatagac acgaggacgg ctgtggagtt gctcaatgat gacgtggagc tgaccacaga
     5281 cttggtgaga gtgcacccgg acagcagcct ggtgggtcgt aagggctaca gtaccactga
     5341 cgggtcgctg tactcgtact ttgaaggtac gaaattcaac caggctgcta ttgatatggc
     5401 agagatactg acgttgtggc ccagactgca agaggcaaac gaacagatat gcctatacgc
     5461 gctgggcgaa acaatggaca acatcagatc caaatgtccg gtgaacgatt ccgattcatc
     5521 aacacctccc aggacagtgc cctgcctgtg ccgctacgca atgacagcag aacggatcac
     5581 ccgccttagg tcacaccaag ttaaaagcat ggtggtttgc tcatcttttc ccctcccgaa
     5641 ataccatgta gatggggtgc agaaggtaaa gtgcgagaag gttctcctgt tcgacccgac
     5701 ggtaccttca gtggttagtc cgcggaagta tgccgcatct acgacggacc actcagatcg
     5761 gtcgttacga gggtttgact tggactggac caccgactcg tcttccactg ccagcgatac
     5821 catgtcgcta cccagtttgc agtcgtgtga catcgactcg atctacgagc caatggctcc
     5881 catagtagtg acggctgacg tacaccctga acccgcaggc atcgcggacc tggcggcaga
     5941 tgtgcatcct gaacccgcag accatgtgga cctcgagaac ccaattcctc caccacgccc
     6001 aaagagagct gcataccttg cctcccgcgc tgcggagcga ccggtgcctg cgcctagaaa
     6061 gcctacgcct gccccaagga ctgcgtttag gaacaagctg cctttgacgt tcggcgactt
     6121 tgacgagcac gaggtcgatg cgttggcctc cgggattact ttcggagact tcgacgacgt
     6181 cctgcgacta ggccgcgcgg gtgcatatat tttctcctcg gacactggca gcggacattt
     6241 acaacaaaaa tccgttaggc agcacaatct ccagtgcgca caactggatg cggtcgagga
     6301 ggagaaaatg tacccgccaa aattggatac tgagagggag aagctgttgc tgctgaaaat
     6361 gcagatgcac ccatcggagg ctaataagag tcgataccag tctcgcaaag tggagaacat
     6421 gaaagccacg gtggtggaca ggctcacatc gggggccaga ttgtacacgg gagcggacgt
     6481 aggccgcata ccaacatacg cggttcggta cccccgcccc gtgtactccc ctaccgtgat
     6541 cgaaagattc tcaagccccg atgtagcaat cgcagcgtgc aacgaatacc tatccagaaa
     6601 ttacccaaca gtggcgtcgt accagataac agatgaatac gacgcatact tggacatggt
     6661 tgacgggtcg gatagttgct tggacagagc gacattctgc ccggcgaagc tccggtgcta
     6721 cccgaaacat catgcgtacc accagccgac tgtacgcagt gccgtcccgt caccctttca
     6781 gaacacacta cagagcgtgc tagcggccgc caccaagaga aactgcaacg tcacgcaaat
     6841 gcgagaacta cccaccatgg actcggcagt gttcaacgtg gagtgcttca agcgctatgc
     6901 ctgctccgga gaatattggg aagaatatgc taaacaacct atccggataa ccactgagaa
     6961 catcactacc tatgtgacca aattgaaagg cccgaaagct gctgccttgt tcgctaagac
     7021 ccacaacttg gttccgctgc aggaggttcc catggacaga ttcacggtcg acatgaaacg
     7081 agatgtcaaa gtcactccag ggacgaaaca cacagaggaa agacccaaag tccaggtaat
     7141 tcaagcagcg gagccattgg cgaccgctta cctgtgcggc atccacaggg aattagtaag
     7201 gagactaaat gctgtgttac gccctaacgt gcacacattg tttgatatgt cggccgaaga
     7261 ctttgacgcg atcatcgcct ctcacttcca cccaggagac ccggttctag agacggacat
     7321 tgcatcattc gacaaaagcc aggacgactc cttggctctt acaggtttaa tgatcctcga
     7381 agatctaggg gtggatcagt acctgctgga cttgatcgag gcagcctttg gggaaatatc
     7441 cagctgtcac ctaccaactg gcacgcgctt caagttcgga gctatgatga aatcgggcat
     7501 gtttctgact ttgtttatta acactgtttt gaacatcacc atagcaagca gggtactgga
     7561 gcagagactc actgactccg cctgtgcggc cttcatcggc gacgacaaca tcgttcacgg
     7621 agtgatctcc gacaagctga tggcggagag gtgcgcgtcg tgggtcaaca tggaggtgaa
     7681 gatcattgac gctgtcatgg gcgaaaaacc cccatatttt tgtgggggat tcatagtttt
     7741 tgacagcgtc acacagaccg cctgccgtgt ttcagaccca cttaagcgcc tgttcaagtt
     7801 gggtaagccg ctaacagctg aagacaagca ggacgaagac aggcgacgag cactgagtga
     7861 cgaggttagc aagtggttcc ggacaggctt gggggccgaa ctggaggtgg cactaacatc
     7921 taggtataag gtagagggct gcaaaagtat cctcatagcc atggccacct tggcgaggga
     7981 cattaaggcg tttaagaaat tgagaggacc tgttatacac ctctacggcg gtcctagatt
     8041 ggtgcgttaa tacacagaat tctgattata gcgcactatt atagcaccat gtctagactg
     8101 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc
     8161 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     8221 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     8281 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     8341 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     8401 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     8461 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     8521 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     8581 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct
     8641 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     8701 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     8761 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     8821 atgctccccg ggtaagttag ggtaggcaat ggcattgata tagcaagaaa attgaaaaca
     8881 gaaaaagtta gggtaagcaa tggcatataa ccataactgt ataacttgta acaaagcgca
     8941 acaagacctg cgcaattggc cccgtggtcc gcctcacgga aactcggggc aactcatatt
     9001 gacacattaa ttggcaataa ttggaagctt acataagctt aattcgacga ataattggat
     9061 ttttatttta ttttgcaatt ggtttttaat atttccaaaa aaaaaaaaaa aaaaaaaaaa
     9121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaactag tctgcattaa
     9181 tgaatcggct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9241 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     9301 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     9361 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     9421 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9481 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9541 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9601 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9661 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9721 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9781 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9841 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9901 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10021 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10081 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10141 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10201 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10261 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
    10321 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
    10381 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
    10441 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
    10501 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
    10561 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
    10621 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
    10681 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
    10741 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
    10801 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
    10861 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
    10921 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    10981 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    11041 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    11101 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    11161 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    11221 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    11281 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    11341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    11401 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    11461 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11521 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11581 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11641 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12301 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
    12421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    12481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    12541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    12601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    12661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    12721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    12781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    12841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    12901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    12961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    13261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    13321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    13381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    13441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    13501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    13561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    13621 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    13681 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    13741 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    13801 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    13861 cgcgtt
//
