LOCUS       Exported               14133 bp ds-DNA     circular SYN 16-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Dr John and Anne Chong Lab for Functional Genomics
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           902..985
                     /label=SFV 5'UTR
                     /note="color: #00ff00"
     misc_feature    986..8281
                     /label=nsP1-4
                     /note="color: #993366"
     CDS             986..2596
                     /codon_start=1
                     /label=nsP1
                     /note="color: #00ccff"
                     /translation="MAAKVHVDIEADSPFIKSLQKAFPSFEVESLQVTPNDHANARAFS
                     HLATKLIEQETDKDTLILDIGSAPSRRMMSTHKYHCVCPMRSAEDPERLVCYAKKLAAA
                     SEKVLDREIAGKITDLQTVMATPDAESPTFCLHTDVTCRTAAEVAVYQDVYAVHAPTSL
                     YHQAMKGVRTAYWIGFDTTPFMFDALAGAYPTYATNWADEQVLQARNIGLCAASLTEGR
                     LGKLSILRKKQLKPCDTVMFSVGSTLYTESRKLLRSWHLPSVFHLKGKQSFTCRCDTIV
                     SCEGYVVKKITMCPGLYGKTVGYAVTYHAEGFLVCKTTDTVKGERVSFPVCTYVPSTIC
                     DQMTGILATDITPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPIVAVAFSKWAREY
                     KADLDDEKPLGVRERSLTCCCLWAFKTRKMHTMYKKPDTQTIVKVPSEFNSFVIPSLWS
                     TGLAIPVRSRIKMLLAKKTKRESIPVLDASSARDAEQEEKERLEAELTREALPPLVPTA
                     PAETGVVDVDVEELEYHAGA"
     misc_feature    1301..1303
                     /label=G106E
                     /note="Middle G base converted to A, creates non-synonymous
                     G-->E. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2024..2026
                     /label=L347L
                     /note="Third position A base converted to G, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2036..2038
                     /label=V351I
                     /note="First position G base converted to A, non-synonymous
                     V-->I mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    2426..2428
                     /product="MUTAITON TO CHANGE L TO S AMINO ACID"
                     /label=L481S
                     /note="Second position T base converted to C, 
                     non-synonymous L-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2531..2533
                     /label=I516T
                     /note="Second position T base converted to C, 
                     non-synonymous I-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     CDS             2597..4993
                     /codon_start=1
                     /label=nsP2
                     /note="color: #ff99cc"
                     /translation="GVVETPRSALKVTAQPNGVLLGNYVVLSPQTVLKSSKLAPVHPLA
                     EQVKIITHNGRAGRYQVDGYDGRVLLPCGSAIPVPEFQALSESATMVYNEREFVNRKLY
                     HIAVHGPSLNTDEENYEKVRAERTDAEYVFDVDKKCCVKREEASGLVLVGELTNPPFHE
                     FAYEGLKIRPSAPYKTTVVGVFGVPGSGKSAIIKSLVTKHDLVTSGKKENCQEIVNDVK
                     KHRGLDIQAKTVDSILLNGCRRAVDILYVDEAFACHSGTLLALIALVKPRSKVVLCGDP
                     KQCGFFNMMQLKVNFNHNICTEVCHKSISRRCTRPVTAIVSTLHYGGKMRTTNPCNKPI
                     IIDTTGQTKPKPGDIVLTCFRGWVKQLQLDYRGHEVMTAAASQGLTRKGVYAVRQKVNE
                     NPLYAPASEHVNVLLTRTEDRLVWKTLAGDPWIKVLSNIPQGNFTATLEEWQEEHDKIM
                     KVIEGPAAPVDAFQNKANVCWAKSLVPVLDTAGIRLTAEEWSTIITAFKEDRAYSPVVA
                     LNEICTKYYGVDLDSGLFSAPKVSLYYENNHWDNRPGGRMYGFNAATAARLEARHTFLK
                     GQWHTGKQAVIAERKIQPLSVLDNVIPINRRLPHALVTEYKTVKGSRVEWLVNKVRGYH
                     VLLVSEYNLALPRRRVTWLSPLNVTGADRCYDLSLGLPRLEGRFDLVFVNIHTEFRIHH
                     YQQCVDHAMKLQMLGGDALRLLKPGGSLLMRAYGYADKISEAVVSSLSRKFSSARVLRP
                     DCVTSNTEVFLLFSNFDNGKRPSTLHQMNTKLSAVYAGEAMHTAGC"
     misc_feature    2648..2650
                     /label=D555G
                     /note="Second position A base converted to G, 
                     non-synonymous D-->G mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3383..3385
                     /label=L800L
                     /note="Third position A represents the synonymous mutation 
                     (G nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3563..3565
                     /label=T860T
                     /note="Third position G base converted to A, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3668..3724
                     /label=approximate packaging sequence
                     /note="PMID 9557722"
                     /note="color: #00ff00"
     misc_feature    4436..4438
                     /label=A1151T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    4616..4624
                     /label=ADA>RLE
                     /note="PMID: 31163352"
                     /note="color: #666699"
     CDS             4994..6439
                     /codon_start=1
                     /label=nsP3
                     /note="color: #ccffcc"
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGEATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDTRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERITRLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKVLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     misc_feature    5459..5461
                     /label=I1492I
                     /note="Third position A represents the synonymous mutation 
                     (T nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5465..5467
                     /product="MUTATE M TO T AMINO ACID"
                     /label=M1494T
                     /note="Second position T base converted to C, 
                     non-synonymous M-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5813..5815
                     /label=A1610T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    6217..6298
                     /label=CODON-OPTIMISED SEQUENCE FOR IDT SYNTHESIS
                     /note="This sequence contains the following 
                     codon-optimisations to reduce GC-content and make it 
                     synthesisable.g6217a, g6229a, g6235a, g6265t, g6283t, 
                     g6289t, g6298t"
                     /note="color: #333333"
     CDS             6440..8281
                     /codon_start=1
                     /label=nsP4
                     /note="color: #ffff99"
                     /translation="YIFSSDTGSGHLQQKSVRQHNLQCAQLDAVEEEKMYPPKLDTERE
                     KLLLLKMQMHPSEANKSRYQSRKVENMKATVVDRLTSGARLYTGADVGRIPTYAVRYPR
                     PVYSPTVIERFSSPDVAIAACNEYLSRNYPTVASYQITDEYDAYLDMVDGSDSCLDRAT
                     FCPAKLRCYPKHHAYHQPTVRSAVPSPFQNTLQSVLAAATKRNCNVTQMRELPTMDSAV
                     FNVECFKRYACSGEYWEEYAKQPIRITTENITTYVTKLKGPKAAALFAKTHNLVPLQEV
                     PMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLRP
                     NVHTLFDMSAEDFDAIIASHFHPGDPVLETDIASFDKSQDDSLALTGLMILEDLGVDQY
                     LLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFINTVLNITIASRVLEQRLTDS
                     ACAAFIGDDNIVHGVISDKLMAERCASWVNMEVKIIDAVMGEKPPYFCGGFIVFDSVTQ
                     TACRVSDPLKRLFKLGKPLTAEDKQDEDRRRALSDEVSKWFRTGLGAELEVALTSRYKV
                     EGCKSILIAMATLARDIKAFKKLRGPVIHLYGGPRLVR"
     misc_feature    7028..7030
                     /label=N2015S
                     /note="Second position A base converted to G, 
                     non-synonymous N-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    8162..8164
                     /label=E2393K
                     /note="First position G base converted to A, non-synonymous
                     E-->K mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    8285..8322
                     /label=SGP
                     /note="color: #666699"
     CDS             8323..9117
                     /codon_start=1
                     /product="aa1-265 of human serum response factor 
                     (representing DNA-binding domain)"
                     /label=SRF
                     /note="color: #ffcc00"
                     /translation="MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVP
                     GNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSE
                     MEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSK
                     RKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSP
                     DSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKP"
     CDS             9130..9177
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9184..9333
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /note="color: #993366"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     3'UTR           9337..9597
                     /label=SFV Viral 3'UTR
                     /note="color: #ff6600"
     polyA_site      9598..9667
                     /label=PolyA Tail
                     /note="color: #------"
     polyA_signal    9733..9957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      10003..10431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        10445..10774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10625..10760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             10841..11635
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    11809..11930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(11979..11995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    12003..12019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(12027..12057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 12027 .. 12033 / #ffffff / -10
                        2: 12034 .. 12051 / #ffffff
                        3: 12052 .. 12057 / #ffffff / -35"
     protein_bind    12072..12093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(12381..12966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(13137..13997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 13137 .. 13928 / #ccffcc
                        2: 13929 .. 13997 / #ccffcc / signal sequence
                     Cleavage site after base 13928"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13998..14102)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggcggatg tgtgacatac acgacgccaa aagattttgt tccagctcct gccacctccg
      961 ctacgcgaga gattaaccac ccacgatggc cgccaaagtg catgttgata ttgaggctga
     1021 cagcccattc atcaagtctt tgcagaaggc atttccgtcg ttcgaggtgg agtcattgca
     1081 ggtcacacca aatgaccatg caaatgccag agcattttcg cacctggcta ccaaattgat
     1141 cgagcaggag actgacaaag acacactcat cttggatatc ggcagtgcgc cttccaggag
     1201 aatgatgtct acgcacaaat accactgcgt atgccctatg cgcagcgcag aagaccccga
     1261 aaggctcgta tgctacgcaa agaaactggc agcggcctcc gagaaggtgc tggatagaga
     1321 gatcgcagga aaaatcaccg acctgcagac cgtcatggct acgccagacg ctgaatctcc
     1381 taccttttgc ctgcatacag acgtcacgtg tcgtacggca gccgaagtgg ccgtatacca
     1441 ggacgtgtat gctgtacatg caccaacatc gctgtaccat caggcgatga aaggtgtcag
     1501 aacggcgtat tggattgggt ttgacaccac cccgtttatg tttgacgcgc tagcaggcgc
     1561 gtatccaacc tacgccacaa actgggccga cgagcaggtg ttacaggcca ggaacatagg
     1621 actgtgtgca gcatccttga ctgagggaag actcggcaaa ctgtccattc tccgcaagaa
     1681 gcaattgaaa ccttgcgaca cagtcatgtt ctcggtagga tctacattgt acactgagag
     1741 cagaaagcta ctgaggagct ggcacttacc ctccgtattc cacctgaaag gtaaacaatc
     1801 ctttacctgt aggtgcgata ccatcgtatc atgtgaaggg tacgtagtta agaaaatcac
     1861 tatgtgcccc ggcctgtacg gtaaaacggt agggtacgcc gtgacgtatc acgcggaggg
     1921 attcctagtg tgcaagacca cagacactgt caaaggagaa agagtctcat tccctgtatg
     1981 cacctacgtc ccctcaacca tctgtgatca aatgactggc atactggcga ccgacatcac
     2041 accggaggac gcacagaagt tgttagtggg attgaatcag aggatagttg tgaacggaag
     2101 aacacagcga aacactaaca cgatgaagaa ctatctgctt ccgattgtgg ccgtcgcatt
     2161 tagcaagtgg gcgagggaat acaaggcaga ccttgatgat gaaaaacctc tgggtgtccg
     2221 agagaggtca cttacttgct gctgcttgtg ggcatttaaa acgaggaaga tgcacaccat
     2281 gtacaagaaa ccagacaccc agacaatagt gaaggtgcct tcagagttta actcgttcgt
     2341 catcccgagc ctatggtcta caggcctcgc aatcccagtc agatcacgca ttaagatgct
     2401 tttggccaag aagaccaagc gagagtcaat acctgttctc gacgcgtcgt cagccaggga
     2461 tgctgaacaa gaggagaagg agaggttgga ggccgagctg actagagaag ccttaccacc
     2521 cctcgtcccc accgcgccgg cggagacggg agtcgtcgac gtcgacgttg aagaactaga
     2581 gtatcacgca ggtgcagggg tcgtggaaac acctcgcagc gcgttgaaag tcaccgcaca
     2641 gccgaacggc gtactactag gaaattacgt agttctgtcc ccgcagaccg tgctcaagag
     2701 ctccaagttg gcccccgtgc accctctagc agagcaggtg aaaataataa cacataacgg
     2761 gagggccggc cgttaccagg tcgacggata tgacggcagg gtcctactac catgtggatc
     2821 ggccattccg gtccctgagt ttcaagcttt gagcgagagc gccactatgg tgtacaacga
     2881 aagggagttc gtcaacagga aactatacca tattgccgtt cacggaccgt cgctgaacac
     2941 cgacgaggag aactacgaga aagtcagagc tgaaagaact gacgccgagt acgtgttcga
     3001 cgtagataaa aaatgctgcg tcaagagaga ggaagcgtcg ggtttggtgt tggtgggaga
     3061 gctaaccaac cccccgttcc atgaattcgc ctacgaaggg ctgaagatca ggccgtcggc
     3121 accatataag actacagtag taggagtctt tggggttccg ggatcaggca agtctgctat
     3181 tattaagagc ctcgtgacca aacacgatct ggtcaccagc ggcaagaagg agaactgcca
     3241 ggaaatagtc aacgacgtga agaagcaccg cggactggac atccaggcaa aaacagtgga
     3301 ctccatcctg ctaaacgggt gtcgtcgtgc cgtggacatc ctatatgtgg acgaggcttt
     3361 cgcttgccat tccggtactc tgctagccct aattgctctt gttaaacctc ggagcaaagt
     3421 ggtgttatgc ggagacccca agcaatgcgg attcttcaat atgatgcagc ttaaggtgaa
     3481 cttcaaccac aacatctgca ctgaagtatg tcataaaagt atatccagac gttgcacgcg
     3541 tccagtcacg gccatcgtgt ctacattgca ctacggaggc aagatgcgca cgaccaaccc
     3601 gtgcaacaaa cccataatca tagacaccac aggacagacc aagcccaagc caggagacat
     3661 cgtgttaaca tgcttccgag gctgggtaaa gcagctgcag ttggactacc gtggacacga
     3721 agtcatgaca gcagcagcat ctcagggcct cacccgcaaa ggggtatacg ccgtaaggca
     3781 gaaggtgaat gaaaatccct tgtatgcccc tgcgtcggag cacgtgaatg tactgctgac
     3841 gcgcactgag gataggctgg tgtggaaaac gctggccggc gatccctgga ttaaggtcct
     3901 atcaaacatt ccacagggta actttacggc cacattggaa gaatggcaag aagaacacga
     3961 caaaataatg aaggtgattg aaggaccggc tgcgcctgtg gacgcgttcc agaacaaagc
     4021 gaacgtgtgt tgggcgaaaa gcctggtgcc tgtcctggac actgccggaa tcagattgac
     4081 agcagaggag tggagcacca taattacagc atttaaggag gacagagctt actctccagt
     4141 ggtggccttg aatgaaattt gcaccaagta ctatggagtt gacctggaca gtggcctgtt
     4201 ttctgccccg aaggtgtccc tgtattacga gaacaaccac tgggataaca gacctggtgg
     4261 aaggatgtat ggattcaatg ccgcaacagc tgccaggctg gaagctagac ataccttcct
     4321 gaaggggcag tggcatacgg gcaagcaggc agttatcgca gaaagaaaaa tccaaccgct
     4381 ttctgtgctg gacaatgtaa ttcctatcaa ccgcaggctg ccgcacgccc tggtgactga
     4441 gtacaagacg gttaaaggca gtagggttga gtggctggtc aataaagtaa gagggtacca
     4501 cgtcctgctg gtgagtgagt acaacctggc tttgcctcga cgcagggtca cttggttgtc
     4561 accgctgaat gtcacaggcg ccgataggtg ctacgaccta agtttaggac tgccgcggct
     4621 ggaaggcagg ttcgacttgg tctttgtgaa cattcacacg gaattcagaa tccaccacta
     4681 ccagcagtgt gtcgaccacg ccatgaagct gcagatgctt gggggagatg cgctacgact
     4741 gctaaaaccc ggcggcagcc tcttgatgag agcttacgga tacgccgata aaatcagcga
     4801 agccgttgtt tcctccttaa gcagaaagtt ctcgtctgca agagtgttgc gcccggattg
     4861 tgtcaccagc aatacagaag tgttcttgct gttctccaac tttgacaacg gaaagagacc
     4921 ctctacgcta caccagatga ataccaagct gagtgccgtg tatgccggag aagccatgca
     4981 cacggccggg tgtgcaccat cctacagagt taagagagca gacatagcca cgtgcacaga
     5041 agcggctgtg gttaacgcag ctaacgcccg tggaactgta ggggatggcg tatgcagggc
     5101 cgtggcgaag aaatggccgt cagcctttaa gggagaagca acaccagtgg gcacaattaa
     5161 aacagtcatg tgcggctcgt accccgtcat ccacgctgta gcgcctaatt tctctgccac
     5221 gactgaagcg gaaggggacc gcgaattggc cgctgtctac cgggcagtgg ccgccgaagt
     5281 aaacagactg tcactgagca gcgtagccat cccgctgctg tccacaggag tgttcagcgg
     5341 cggaagagat aggctgcagc aatccctcaa ccatctattc acagcaatgg acgccacgga
     5401 cgctgacgtg accatctact gcagagacaa aagttgggag aagaaaatcc aggaagccat
     5461 agacacgagg acggctgtgg agttgctcaa tgatgacgtg gagctgacca cagacttggt
     5521 gagagtgcac ccggacagca gcctggtggg tcgtaagggc tacagtacca ctgacgggtc
     5581 gctgtactcg tactttgaag gtacgaaatt caaccaggct gctattgata tggcagagat
     5641 actgacgttg tggcccagac tgcaagaggc aaacgaacag atatgcctat acgcgctggg
     5701 cgaaacaatg gacaacatca gatccaaatg tccggtgaac gattccgatt catcaacacc
     5761 tcccaggaca gtgccctgcc tgtgccgcta cgcaatgaca gcagaacgga tcacccgcct
     5821 taggtcacac caagttaaaa gcatggtggt ttgctcatct tttcccctcc cgaaatacca
     5881 tgtagatggg gtgcagaagg taaagtgcga gaaggttctc ctgttcgacc cgacggtacc
     5941 ttcagtggtt agtccgcgga agtatgccgc atctacgacg gaccactcag atcggtcgtt
     6001 acgagggttt gacttggact ggaccaccga ctcgtcttcc actgccagcg ataccatgtc
     6061 gctacccagt ttgcagtcgt gtgacatcga ctcgatctac gagccaatgg ctcccatagt
     6121 agtgacggct gacgtacacc ctgaacccgc aggcatcgcg gacctggcgg cagatgtgca
     6181 tcctgaaccc gcagaccatg tggacctcga gaacccaatt cctccaccac gcccaaagag
     6241 agctgcatac cttgcctccc gcgctgcgga gcgaccggtg cctgcgccta gaaagcctac
     6301 gcctgcccca aggactgcgt ttaggaacaa gctgcctttg acgttcggcg actttgacga
     6361 gcacgaggtc gatgcgttgg cctccgggat tactttcgga gacttcgacg acgtcctgcg
     6421 actaggccgc gcgggtgcat atattttctc ctcggacact ggcagcggac atttacaaca
     6481 aaaatccgtt aggcagcaca atctccagtg cgcacaactg gatgcggtcg aggaggagaa
     6541 aatgtacccg ccaaaattgg atactgagag ggagaagctg ttgctgctga aaatgcagat
     6601 gcacccatcg gaggctaata agagtcgata ccagtctcgc aaagtggaga acatgaaagc
     6661 cacggtggtg gacaggctca catcgggggc cagattgtac acgggagcgg acgtaggccg
     6721 cataccaaca tacgcggttc ggtacccccg ccccgtgtac tcccctaccg tgatcgaaag
     6781 attctcaagc cccgatgtag caatcgcagc gtgcaacgaa tacctatcca gaaattaccc
     6841 aacagtggcg tcgtaccaga taacagatga atacgacgca tacttggaca tggttgacgg
     6901 gtcggatagt tgcttggaca gagcgacatt ctgcccggcg aagctccggt gctacccgaa
     6961 acatcatgcg taccaccagc cgactgtacg cagtgccgtc ccgtcaccct ttcagaacac
     7021 actacagagc gtgctagcgg ccgccaccaa gagaaactgc aacgtcacgc aaatgcgaga
     7081 actacccacc atggactcgg cagtgttcaa cgtggagtgc ttcaagcgct atgcctgctc
     7141 cggagaatat tgggaagaat atgctaaaca acctatccgg ataaccactg agaacatcac
     7201 tacctatgtg accaaattga aaggcccgaa agctgctgcc ttgttcgcta agacccacaa
     7261 cttggttccg ctgcaggagg ttcccatgga cagattcacg gtcgacatga aacgagatgt
     7321 caaagtcact ccagggacga aacacacaga ggaaagaccc aaagtccagg taattcaagc
     7381 agcggagcca ttggcgaccg cttacctgtg cggcatccac agggaattag taaggagact
     7441 aaatgctgtg ttacgcccta acgtgcacac attgtttgat atgtcggccg aagactttga
     7501 cgcgatcatc gcctctcact tccacccagg agacccggtt ctagagacgg acattgcatc
     7561 attcgacaaa agccaggacg actccttggc tcttacaggt ttaatgatcc tcgaagatct
     7621 aggggtggat cagtacctgc tggacttgat cgaggcagcc tttggggaaa tatccagctg
     7681 tcacctacca actggcacgc gcttcaagtt cggagctatg atgaaatcgg gcatgtttct
     7741 gactttgttt attaacactg ttttgaacat caccatagca agcagggtac tggagcagag
     7801 actcactgac tccgcctgtg cggccttcat cggcgacgac aacatcgttc acggagtgat
     7861 ctccgacaag ctgatggcgg agaggtgcgc gtcgtgggtc aacatggagg tgaagatcat
     7921 tgacgctgtc atgggcgaaa aacccccata tttttgtggg ggattcatag tttttgacag
     7981 cgtcacacag accgcctgcc gtgtttcaga cccacttaag cgcctgttca agttgggtaa
     8041 gccgctaaca gctgaagaca agcaggacga agacaggcga cgagcactga gtgacgaggt
     8101 tagcaagtgg ttccggacag gcttgggggc cgaactggag gtggcactaa catctaggta
     8161 taaggtagag ggctgcaaaa gtatcctcat agccatggcc accttggcga gggacattaa
     8221 ggcgtttaag aaattgagag gacctgttat acacctctac ggcggtccta gattggtgcg
     8281 ttaatacaca gaattctgat tatagcgcac tattatagca ccatgctccc tacccaggct
     8341 ggcgctgctg ccgctctggg cagaggctct gctctgggag gctctctgaa ccggacccct
     8401 accggcagac ctggaggcgg cggcggcacc cggggcgcca acggaggcag agtgcctggc
     8461 aatggcgctg gtctgggccc aggccggctg gaaagagagg ccgctgccgc cgccgccaca
     8521 acccctgctc caaccgccgg cgctctgtat agcggatccg agggcgattc cgagtctggc
     8581 gaggaagagg aactgggcgc tgagcggaga ggcctgaaga gatccctgtc tgagatggaa
     8641 atcggcatgg tggtgggcgg acctgaggcc tccgctgctg ctacaggagg ctacggccct
     8701 gtgtctggcg ccgtgtccgg cgccaagcct ggcaagaaaa cccgcggaag agtcaagatc
     8761 aagatggaat tcatcgacaa caagctgaga agatacacca ccttctccaa gaggaaaaca
     8821 ggcatcatga agaaggccta cgagctgtct accctgacag gcacccaggt gctgctgctg
     8881 gtggcttctg agactggaca cgtgtacacc tttgccacac ggaaactgca acctatgatc
     8941 acctctgaaa ccggaaaggc cctgatccag acctgcctga attctccaga ttcccctcct
     9001 agatctgatc ctaccacaga tcagcggatg tctgccaccg gatttgagga aaccgacctg
     9061 acctaccaag tgtccgagtc cgactcctct ggcgagacaa aggacaccct caagccatca
     9121 ggaagtggga agcgtcctgc tgctacaaaa aaagctggac aggctaagaa aaaaaaaggc
     9181 tctgatgcac tggatgattt tgatctcgat atgctgggaa gcgatgccct tgatgacttt
     9241 gatctggaca tgctgggatc cgatgctctt gacgacttcg acttggatat gttggggtcc
     9301 gatgcacttg atgattttga ccttgacatg ctgtaagtta gggtaggcaa tggcattgat
     9361 atagcaagaa aattgaaaac agaaaaagtt agggtaagca atggcatata accataactg
     9421 tataacttgt aacaaagcgc aacaagacct gcgcaattgg ccccgtggtc cgcctcacgg
     9481 aaactcgggg caactcatat tgacacatta attggcaata attggaagct tacataagct
     9541 taattcgacg aataattgga tttttatttt attttgcaat tggtttttaa tatttccaaa
     9601 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9661 aaaaaaacta gtctgcatta atgaatcggc tcgagtctag agggcccgtt taaacccgct
     9721 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9781 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9841 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9901 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9961 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    10021 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10081 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10141 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10201 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10261 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10321 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10381 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    10441 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10501 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10561 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10621 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10681 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10741 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10801 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
    10861 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
    10921 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
    10981 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
    11041 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    11101 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    11161 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
    11221 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
    11281 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
    11341 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
    11401 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
    11461 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
    11521 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
    11581 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
    11641 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
    11701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    11761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    11821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    11941 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    12001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    12061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    12121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    12181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    12241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    12301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    12361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    12421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    12481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    12541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    12601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    12841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12901 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    12961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    13081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    13141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    13201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    13801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    13861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    13921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    13981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    14041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    14101 cgcacatttc cccgaaaagt gccacctgac gtc
//
