LOCUS       Exported               14127 bp ds-DNA     circular SYN 20-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Dr John and Anne Chong Lab for Functional Genomics
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           668..751
                     /label=SFV 5'UTR
                     /note="color: #00ff00"
     misc_feature    752..8047
                     /label=nsP1-4
                     /note="color: #993366"
     CDS             752..2362
                     /codon_start=1
                     /label=nsP1
                     /note="color: #00ccff"
                     /translation="MAAKVHVDIEADSPFIKSLQKAFPSFEVESLQVTPNDHANARAFS
                     HLATKLIEQETDKDTLILDIGSAPSRRMMSTHKYHCVCPMRSAEDPERLVCYAKKLAAA
                     SEKVLDREIAGKITDLQTVMATPDAESPTFCLHTDVTCRTAAEVAVYQDVYAVHAPTSL
                     YHQAMKGVRTAYWIGFDTTPFMFDALAGAYPTYATNWADEQVLQARNIGLCAASLTEGR
                     LGKLSILRKKQLKPCDTVMFSVGSTLYTESRKLLRSWHLPSVFHLKGKQSFTCRCDTIV
                     SCEGYVVKKITMCPGLYGKTVGYAVTYHAEGFLVCKTTDTVKGERVSFPVCTYVPSTIC
                     DQMTGILATDITPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPIVAVAFSKWAREY
                     KADLDDEKPLGVRERSLTCCCLWAFKTRKMHTMYKKPDTQTIVKVPSEFNSFVIPSLWS
                     TGLAIPVRSRIKMLLAKKTKRESIPVLDASSARDAEQEEKERLEAELTREALPPLVPTA
                     PAETGVVDVDVEELEYHAGA"
     misc_feature    1067..1069
                     /label=G106E
                     /note="Middle G base converted to A, creates non-synonymous
                     G-->E. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1790..1792
                     /label=L347L
                     /note="Third position A base converted to G, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    1802..1804
                     /label=V351I
                     /note="First position G base converted to A, non-synonymous
                     V-->I mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    2192..2194
                     /product="MUTAITON TO CHANGE L TO S AMINO ACID"
                     /label=L481S
                     /note="Second position T base converted to C, 
                     non-synonymous L-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    2297..2299
                     /label=I516T
                     /note="Second position T base converted to C, 
                     non-synonymous I-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     CDS             2363..4759
                     /codon_start=1
                     /label=nsP2
                     /note="color: #ff99cc"
                     /translation="GVVETPRSALKVTAQPNGVLLGNYVVLSPQTVLKSSKLAPVHPLA
                     EQVKIITHNGRAGRYQVDGYDGRVLLPCGSAIPVPEFQALSESATMVYNEREFVNRKLY
                     HIAVHGPSLNTDEENYEKVRAERTDAEYVFDVDKKCCVKREEASGLVLVGELTNPPFHE
                     FAYEGLKIRPSAPYKTTVVGVFGVPGSGKSAIIKSLVTKHDLVTSGKKENCQEIVNDVK
                     KHRGLDIQAKTVDSILLNGCRRAVDILYVDEAFACHSGTLLALIALVKPRSKVVLCGDP
                     KQCGFFNMMQLKVNFNHNICTEVCHKSISRRCTRPVTAIVSTLHYGGKMRTTNPCNKPI
                     IIDTTGQTKPKPGDIVLTCFRGWVKQLQLDYRGHEVMTAAASQGLTRKGVYAVRQKVNE
                     NPLYAPASEHVNVLLTRTEDRLVWKTLAGDPWIKVLSNIPQGNFTATLEEWQEEHDKIM
                     KVIEGPAAPVDAFQNKANVCWAKSLVPVLDTAGIRLTAEEWSTIITAFKEDRAYSPVVA
                     LNEICTKYYGVDLDSGLFSAPKVSLYYENNHWDNRPGGRMYGFNAATAARLEARHTFLK
                     GQWHTGKQAVIAERKIQPLSVLDNVIPINRRLPHALVTEYKTVKGSRVEWLVNKVRGYH
                     VLLVSEYNLALPRRRVTWLSPLNVTGADRCYDLSLGLPRLEGRFDLVFVNIHTEFRIHH
                     YQQCVDHAMKLQMLGGDALRLLKPGGSLLMRAYGYADKISEAVVSSLSRKFSSARVLRP
                     DCVTSNTEVFLLFSNFDNGKRPSTLHQMNTKLSAVYAGEAMHTAGC"
     misc_feature    2414..2416
                     /label=D555G
                     /note="Second position A base converted to G, 
                     non-synonymous D-->G mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3149..3151
                     /label=L800L
                     /note="Third position A represents the synonymous mutation 
                     (G nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3329..3331
                     /label=T860T
                     /note="Third position G base converted to A, synonymous 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    3434..3490
                     /label=approximate packaging sequence
                     /note="PMID 9557722"
                     /note="color: #00ff00"
     misc_feature    4202..4204
                     /label=A1151T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    4382..4390
                     /label=ADA>RLE
                     /note="PMID: 31163352"
                     /note="color: #666699"
     CDS             4760..6205
                     /codon_start=1
                     /label=nsP3
                     /note="color: #ccffcc"
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGEATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDTRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERITRLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKVLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     misc_feature    5225..5227
                     /label=I1492I
                     /note="Third position A represents the synonymous mutation 
                     (T nucleotide to A nucleotide) found by Rose et al. We have
                     no need to adjust this, but will label as a site of 
                     mutation. (Derived from Rose et al., PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5231..5233
                     /product="MUTATE M TO T AMINO ACID"
                     /label=M1494T
                     /note="Second position T base converted to C, 
                     non-synonymous M-->T mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    5579..5581
                     /label=A1610T
                     /note="First position G base converted to A, non-synonymous
                     A-->T mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    5983..6064
                     /label=CODON-OPTIMISED SEQUENCE FOR IDT SYNTHESIS
                     /note="This sequence contains the following 
                     codon-optimisations to reduce GC-content and make it 
                     synthesisable.g6217a, g6229a, g6235a, g6265t, g6283t, 
                     g6289t, g6298t"
                     /note="color: #333333"
     CDS             6206..8047
                     /codon_start=1
                     /label=nsP4
                     /note="color: #ffff99"
                     /translation="YIFSSDTGSGHLQQKSVRQHNLQCAQLDAVEEEKMYPPKLDTERE
                     KLLLLKMQMHPSEANKSRYQSRKVENMKATVVDRLTSGARLYTGADVGRIPTYAVRYPR
                     PVYSPTVIERFSSPDVAIAACNEYLSRNYPTVASYQITDEYDAYLDMVDGSDSCLDRAT
                     FCPAKLRCYPKHHAYHQPTVRSAVPSPFQNTLQSVLAAATKRNCNVTQMRELPTMDSAV
                     FNVECFKRYACSGEYWEEYAKQPIRITTENITTYVTKLKGPKAAALFAKTHNLVPLQEV
                     PMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLRP
                     NVHTLFDMSAEDFDAIIASHFHPGDPVLETDIASFDKSQDDSLALTGLMILEDLGVDQY
                     LLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFINTVLNITIASRVLEQRLTDS
                     ACAAFIGDDNIVHGVISDKLMAERCASWVNMEVKIIDAVMGEKPPYFCGGFIVFDSVTQ
                     TACRVSDPLKRLFKLGKPLTAEDKQDEDRRRALSDEVSKWFRTGLGAELEVALTSRYKV
                     EGCKSILIAMATLARDIKAFKKLRGPVIHLYGGPRLVR"
     misc_feature    6794..6796
                     /label=N2015S
                     /note="Second position A base converted to G, 
                     non-synonymous N-->S mutation. (Derived from Rose et al., 
                     PubMed 25385608)."
                     /note="color: #00ccff"
     misc_feature    7928..7930
                     /label=E2393K
                     /note="First position G base converted to A, non-synonymous
                     E-->K mutation. (Derived from Rose et al., PubMed 
                     25385608)."
                     /note="color: #00ccff"
     misc_feature    8051..8088
                     /label=SGP
                     /note="color: #666699"
     CDS             8089..9096
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /note="This feature has 2 segments:
                        1: 8089 .. 8709 / #33cccc / TetR
                        2: 8710 .. 9096 / #999999 / VP16"
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     3'UTR           9097..9357
                     /label=SFV Viral 3'UTR
                     /note="color: #ff6600"
     polyA_site      9358..9427
                     /label=PolyA Tail
                     /note="color: #------"
     polyA_signal    9493..9717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      9763..10191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        10205..10534
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10385..10520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             10601..11395
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    11569..11690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(11739..11755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    11763..11779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(11787..11817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 11787 .. 11793 / #ffffff / -10
                        2: 11794 .. 11811 / #ffffff
                        3: 11812 .. 11817 / #ffffff / -35"
     protein_bind    11832..11853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(12141..12726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12897..13757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 12897 .. 13688 / #ccffcc
                        2: 13689 .. 13757 / #ccffcc / signal sequence
                     Cleavage site after base 13688"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13758..13862)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgg cggatgtgtg acatacacga cgccaaaaga ttttgttcca gctcctgcca
      721 cctccgctac gcgagagatt aaccacccac gatggccgcc aaagtgcatg ttgatattga
      781 ggctgacagc ccattcatca agtctttgca gaaggcattt ccgtcgttcg aggtggagtc
      841 attgcaggtc acaccaaatg accatgcaaa tgccagagca ttttcgcacc tggctaccaa
      901 attgatcgag caggagactg acaaagacac actcatcttg gatatcggca gtgcgccttc
      961 caggagaatg atgtctacgc acaaatacca ctgcgtatgc cctatgcgca gcgcagaaga
     1021 ccccgaaagg ctcgtatgct acgcaaagaa actggcagcg gcctccgaga aggtgctgga
     1081 tagagagatc gcaggaaaaa tcaccgacct gcagaccgtc atggctacgc cagacgctga
     1141 atctcctacc ttttgcctgc atacagacgt cacgtgtcgt acggcagccg aagtggccgt
     1201 ataccaggac gtgtatgctg tacatgcacc aacatcgctg taccatcagg cgatgaaagg
     1261 tgtcagaacg gcgtattgga ttgggtttga caccaccccg tttatgtttg acgcgctagc
     1321 aggcgcgtat ccaacctacg ccacaaactg ggccgacgag caggtgttac aggccaggaa
     1381 cataggactg tgtgcagcat ccttgactga gggaagactc ggcaaactgt ccattctccg
     1441 caagaagcaa ttgaaacctt gcgacacagt catgttctcg gtaggatcta cattgtacac
     1501 tgagagcaga aagctactga ggagctggca cttaccctcc gtattccacc tgaaaggtaa
     1561 acaatccttt acctgtaggt gcgataccat cgtatcatgt gaagggtacg tagttaagaa
     1621 aatcactatg tgccccggcc tgtacggtaa aacggtaggg tacgccgtga cgtatcacgc
     1681 ggagggattc ctagtgtgca agaccacaga cactgtcaaa ggagaaagag tctcattccc
     1741 tgtatgcacc tacgtcccct caaccatctg tgatcaaatg actggcatac tggcgaccga
     1801 catcacaccg gaggacgcac agaagttgtt agtgggattg aatcagagga tagttgtgaa
     1861 cggaagaaca cagcgaaaca ctaacacgat gaagaactat ctgcttccga ttgtggccgt
     1921 cgcatttagc aagtgggcga gggaatacaa ggcagacctt gatgatgaaa aacctctggg
     1981 tgtccgagag aggtcactta cttgctgctg cttgtgggca tttaaaacga ggaagatgca
     2041 caccatgtac aagaaaccag acacccagac aatagtgaag gtgccttcag agtttaactc
     2101 gttcgtcatc ccgagcctat ggtctacagg cctcgcaatc ccagtcagat cacgcattaa
     2161 gatgcttttg gccaagaaga ccaagcgaga gtcaatacct gttctcgacg cgtcgtcagc
     2221 cagggatgct gaacaagagg agaaggagag gttggaggcc gagctgacta gagaagcctt
     2281 accacccctc gtccccaccg cgccggcgga gacgggagtc gtcgacgtcg acgttgaaga
     2341 actagagtat cacgcaggtg caggggtcgt ggaaacacct cgcagcgcgt tgaaagtcac
     2401 cgcacagccg aacggcgtac tactaggaaa ttacgtagtt ctgtccccgc agaccgtgct
     2461 caagagctcc aagttggccc ccgtgcaccc tctagcagag caggtgaaaa taataacaca
     2521 taacgggagg gccggccgtt accaggtcga cggatatgac ggcagggtcc tactaccatg
     2581 tggatcggcc attccggtcc ctgagtttca agctttgagc gagagcgcca ctatggtgta
     2641 caacgaaagg gagttcgtca acaggaaact ataccatatt gccgttcacg gaccgtcgct
     2701 gaacaccgac gaggagaact acgagaaagt cagagctgaa agaactgacg ccgagtacgt
     2761 gttcgacgta gataaaaaat gctgcgtcaa gagagaggaa gcgtcgggtt tggtgttggt
     2821 gggagagcta accaaccccc cgttccatga attcgcctac gaagggctga agatcaggcc
     2881 gtcggcacca tataagacta cagtagtagg agtctttggg gttccgggat caggcaagtc
     2941 tgctattatt aagagcctcg tgaccaaaca cgatctggtc accagcggca agaaggagaa
     3001 ctgccaggaa atagtcaacg acgtgaagaa gcaccgcgga ctggacatcc aggcaaaaac
     3061 agtggactcc atcctgctaa acgggtgtcg tcgtgccgtg gacatcctat atgtggacga
     3121 ggctttcgct tgccattccg gtactctgct agccctaatt gctcttgtta aacctcggag
     3181 caaagtggtg ttatgcggag accccaagca atgcggattc ttcaatatga tgcagcttaa
     3241 ggtgaacttc aaccacaaca tctgcactga agtatgtcat aaaagtatat ccagacgttg
     3301 cacgcgtcca gtcacggcca tcgtgtctac attgcactac ggaggcaaga tgcgcacgac
     3361 caacccgtgc aacaaaccca taatcataga caccacagga cagaccaagc ccaagccagg
     3421 agacatcgtg ttaacatgct tccgaggctg ggtaaagcag ctgcagttgg actaccgtgg
     3481 acacgaagtc atgacagcag cagcatctca gggcctcacc cgcaaagggg tatacgccgt
     3541 aaggcagaag gtgaatgaaa atcccttgta tgcccctgcg tcggagcacg tgaatgtact
     3601 gctgacgcgc actgaggata ggctggtgtg gaaaacgctg gccggcgatc cctggattaa
     3661 ggtcctatca aacattccac agggtaactt tacggccaca ttggaagaat ggcaagaaga
     3721 acacgacaaa ataatgaagg tgattgaagg accggctgcg cctgtggacg cgttccagaa
     3781 caaagcgaac gtgtgttggg cgaaaagcct ggtgcctgtc ctggacactg ccggaatcag
     3841 attgacagca gaggagtgga gcaccataat tacagcattt aaggaggaca gagcttactc
     3901 tccagtggtg gccttgaatg aaatttgcac caagtactat ggagttgacc tggacagtgg
     3961 cctgttttct gccccgaagg tgtccctgta ttacgagaac aaccactggg ataacagacc
     4021 tggtggaagg atgtatggat tcaatgccgc aacagctgcc aggctggaag ctagacatac
     4081 cttcctgaag gggcagtggc atacgggcaa gcaggcagtt atcgcagaaa gaaaaatcca
     4141 accgctttct gtgctggaca atgtaattcc tatcaaccgc aggctgccgc acgccctggt
     4201 gactgagtac aagacggtta aaggcagtag ggttgagtgg ctggtcaata aagtaagagg
     4261 gtaccacgtc ctgctggtga gtgagtacaa cctggctttg cctcgacgca gggtcacttg
     4321 gttgtcaccg ctgaatgtca caggcgccga taggtgctac gacctaagtt taggactgcc
     4381 gcggctggaa ggcaggttcg acttggtctt tgtgaacatt cacacggaat tcagaatcca
     4441 ccactaccag cagtgtgtcg accacgccat gaagctgcag atgcttgggg gagatgcgct
     4501 acgactgcta aaacccggcg gcagcctctt gatgagagct tacggatacg ccgataaaat
     4561 cagcgaagcc gttgtttcct ccttaagcag aaagttctcg tctgcaagag tgttgcgccc
     4621 ggattgtgtc accagcaata cagaagtgtt cttgctgttc tccaactttg acaacggaaa
     4681 gagaccctct acgctacacc agatgaatac caagctgagt gccgtgtatg ccggagaagc
     4741 catgcacacg gccgggtgtg caccatccta cagagttaag agagcagaca tagccacgtg
     4801 cacagaagcg gctgtggtta acgcagctaa cgcccgtgga actgtagggg atggcgtatg
     4861 cagggccgtg gcgaagaaat ggccgtcagc ctttaaggga gaagcaacac cagtgggcac
     4921 aattaaaaca gtcatgtgcg gctcgtaccc cgtcatccac gctgtagcgc ctaatttctc
     4981 tgccacgact gaagcggaag gggaccgcga attggccgct gtctaccggg cagtggccgc
     5041 cgaagtaaac agactgtcac tgagcagcgt agccatcccg ctgctgtcca caggagtgtt
     5101 cagcggcgga agagataggc tgcagcaatc cctcaaccat ctattcacag caatggacgc
     5161 cacggacgct gacgtgacca tctactgcag agacaaaagt tgggagaaga aaatccagga
     5221 agccatagac acgaggacgg ctgtggagtt gctcaatgat gacgtggagc tgaccacaga
     5281 cttggtgaga gtgcacccgg acagcagcct ggtgggtcgt aagggctaca gtaccactga
     5341 cgggtcgctg tactcgtact ttgaaggtac gaaattcaac caggctgcta ttgatatggc
     5401 agagatactg acgttgtggc ccagactgca agaggcaaac gaacagatat gcctatacgc
     5461 gctgggcgaa acaatggaca acatcagatc caaatgtccg gtgaacgatt ccgattcatc
     5521 aacacctccc aggacagtgc cctgcctgtg ccgctacgca atgacagcag aacggatcac
     5581 ccgccttagg tcacaccaag ttaaaagcat ggtggtttgc tcatcttttc ccctcccgaa
     5641 ataccatgta gatggggtgc agaaggtaaa gtgcgagaag gttctcctgt tcgacccgac
     5701 ggtaccttca gtggttagtc cgcggaagta tgccgcatct acgacggacc actcagatcg
     5761 gtcgttacga gggtttgact tggactggac caccgactcg tcttccactg ccagcgatac
     5821 catgtcgcta cccagtttgc agtcgtgtga catcgactcg atctacgagc caatggctcc
     5881 catagtagtg acggctgacg tacaccctga acccgcaggc atcgcggacc tggcggcaga
     5941 tgtgcatcct gaacccgcag accatgtgga cctcgagaac ccaattcctc caccacgccc
     6001 aaagagagct gcataccttg cctcccgcgc tgcggagcga ccggtgcctg cgcctagaaa
     6061 gcctacgcct gccccaagga ctgcgtttag gaacaagctg cctttgacgt tcggcgactt
     6121 tgacgagcac gaggtcgatg cgttggcctc cgggattact ttcggagact tcgacgacgt
     6181 cctgcgacta ggccgcgcgg gtgcatatat tttctcctcg gacactggca gcggacattt
     6241 acaacaaaaa tccgttaggc agcacaatct ccagtgcgca caactggatg cggtcgagga
     6301 ggagaaaatg tacccgccaa aattggatac tgagagggag aagctgttgc tgctgaaaat
     6361 gcagatgcac ccatcggagg ctaataagag tcgataccag tctcgcaaag tggagaacat
     6421 gaaagccacg gtggtggaca ggctcacatc gggggccaga ttgtacacgg gagcggacgt
     6481 aggccgcata ccaacatacg cggttcggta cccccgcccc gtgtactccc ctaccgtgat
     6541 cgaaagattc tcaagccccg atgtagcaat cgcagcgtgc aacgaatacc tatccagaaa
     6601 ttacccaaca gtggcgtcgt accagataac agatgaatac gacgcatact tggacatggt
     6661 tgacgggtcg gatagttgct tggacagagc gacattctgc ccggcgaagc tccggtgcta
     6721 cccgaaacat catgcgtacc accagccgac tgtacgcagt gccgtcccgt caccctttca
     6781 gaacacacta cagagcgtgc tagcggccgc caccaagaga aactgcaacg tcacgcaaat
     6841 gcgagaacta cccaccatgg actcggcagt gttcaacgtg gagtgcttca agcgctatgc
     6901 ctgctccgga gaatattggg aagaatatgc taaacaacct atccggataa ccactgagaa
     6961 catcactacc tatgtgacca aattgaaagg cccgaaagct gctgccttgt tcgctaagac
     7021 ccacaacttg gttccgctgc aggaggttcc catggacaga ttcacggtcg acatgaaacg
     7081 agatgtcaaa gtcactccag ggacgaaaca cacagaggaa agacccaaag tccaggtaat
     7141 tcaagcagcg gagccattgg cgaccgctta cctgtgcggc atccacaggg aattagtaag
     7201 gagactaaat gctgtgttac gccctaacgt gcacacattg tttgatatgt cggccgaaga
     7261 ctttgacgcg atcatcgcct ctcacttcca cccaggagac ccggttctag agacggacat
     7321 tgcatcattc gacaaaagcc aggacgactc cttggctctt acaggtttaa tgatcctcga
     7381 agatctaggg gtggatcagt acctgctgga cttgatcgag gcagcctttg gggaaatatc
     7441 cagctgtcac ctaccaactg gcacgcgctt caagttcgga gctatgatga aatcgggcat
     7501 gtttctgact ttgtttatta acactgtttt gaacatcacc atagcaagca gggtactgga
     7561 gcagagactc actgactccg cctgtgcggc cttcatcggc gacgacaaca tcgttcacgg
     7621 agtgatctcc gacaagctga tggcggagag gtgcgcgtcg tgggtcaaca tggaggtgaa
     7681 gatcattgac gctgtcatgg gcgaaaaacc cccatatttt tgtgggggat tcatagtttt
     7741 tgacagcgtc acacagaccg cctgccgtgt ttcagaccca cttaagcgcc tgttcaagtt
     7801 gggtaagccg ctaacagctg aagacaagca ggacgaagac aggcgacgag cactgagtga
     7861 cgaggttagc aagtggttcc ggacaggctt gggggccgaa ctggaggtgg cactaacatc
     7921 taggtataag gtagagggct gcaaaagtat cctcatagcc atggccacct tggcgaggga
     7981 cattaaggcg tttaagaaat tgagaggacc tgttatacac ctctacggcg gtcctagatt
     8041 ggtgcgttaa tacacagaat tctgattata gcgcactatt atagcaccat ggctagatta
     8101 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     8161 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     8221 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     8281 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     8341 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     8401 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     8461 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     8521 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     8581 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     8641 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     8701 agtgggtccg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac catcgagggc
     8761 ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg
     8821 tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc
     8881 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
     8941 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc
     9001 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt
     9061 accgatgccc ttggaattga cgagtacggt gggtaggtta gggtaggcaa tggcattgat
     9121 atagcaagaa aattgaaaac agaaaaagtt agggtaagca atggcatata accataactg
     9181 tataacttgt aacaaagcgc aacaagacct gcgcaattgg ccccgtggtc cgcctcacgg
     9241 aaactcgggg caactcatat tgacacatta attggcaata attggaagct tacataagct
     9301 taattcgacg aataattgga tttttatttt attttgcaat tggtttttaa tatttccaaa
     9361 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9421 aaaaaaacta gtctgcatta atgaatcggc tcgagtctag agggcccgtt taaacccgct
     9481 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9541 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9601 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9661 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9721 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     9781 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9841 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9901 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9961 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10021 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10081 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    10201 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10261 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10381 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10441 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10501 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10561 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
    10621 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
    10681 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
    10741 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
    10801 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    10861 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    10921 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
    10981 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
    11041 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
    11101 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
    11161 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
    11221 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
    11281 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
    11341 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
    11401 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
    11461 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    11521 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    11581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    11701 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    11761 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11821 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11881 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11941 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    12001 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    12061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    12121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    12181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    12241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    12301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    12361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    12601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12661 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    12721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    12781 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    12841 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    12901 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    12961 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13021 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13081 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13141 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13201 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13261 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13321 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13381 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13441 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13501 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    13561 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    13621 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    13681 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    13741 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    13801 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    13861 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    13921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    13981 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    14041 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    14101 gcgatgtacg ggccagatat acgcgtt
//
