LOCUS       Exported                6154 bp ds-DNA     circular SYN 20-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Dr John and Anne Chong Lab for Functional Genomics
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        113..227
                     /label=SRE
                     /label=SRE; serum response element
                     /note="color: #ff9ccd"
     regulatory      260..290
                     /label=minP
                     /label=minP; minimal promoter
                     /note="color: #faac61"
     intron          425..997
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
                     /note="color: #------"
     CDS             1083..2616
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /note="This feature has 2 segments:
                        1: 1083 .. 1130 / #993366 / signal peptide
                        2: 1131 .. 2616 / #993366"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     3'UTR           2619..2718
                     /label=VSV-G 3'UTR (must keep with VSV-G CDS if cloning)
                     /note="color: #008000"
     polyA_signal    2910..2965
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     primer_bind     complement(3323..3339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    3347..3363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(3371..3401)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 3371 .. 3377 / #ffffff / -10
                        2: 3378 .. 3395 / #ffffff
                        3: 3396 .. 3401 / #ffffff / -35"
     protein_bind    3416..3437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(3725..4313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(4484..5344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4484 .. 5275 / #ccffcc
                        2: 5276 .. 5344 / #ccffcc / signal sequence
                     Cleavage site after base 5275"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5345..5449)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(5475..5930)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     6072..6088
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        6098..6116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg tttcgcgatg tacgggccag atatacgcta gcaggatgtc
      121 catattagga catctaggat gtccatatta ggacatctag gatgtccata ttaggacatc
      181 taggatgtcc atattaggac atctaggatg tccatattag gacatctaga tctggcctcg
      241 gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc
      301 cggtactggg atccgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      361 tgacctccat agaagacacc gggaccgatc cagcctccgg tcgaccgatc ctgagaactt
      421 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
      481 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
      541 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
      601 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
      661 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
      721 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
      781 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
      841 tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt
      901 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
      961 tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt
     1021 tgtgctgtct catcattttg gcaaagaatt cctcgacgga tccctcgagg aattctgaca
     1081 ctatgaagtg ccttttgtac ttagcctttt tattcattgg ggtgaattgc aagttcacca
     1141 tagtttttcc acacaaccaa aaaggaaact ggaaaaatgt tccttctaat taccattatt
     1201 gcccgtcaag ctcagattta aattggcata atgacttaat aggcacagcc ttacaagtca
     1261 aaatgcccaa gagtcacaag gctattcaag cagacggttg gatgtgtcat gcttccaaat
     1321 gggtcactac ttgtgatttc cgctggtatg gaccgaagta tataacacat tccatccgat
     1381 ccttcactcc atctgtagaa caatgcaagg aaagcattga acaaacgaaa caaggaactt
     1441 ggctgaatcc aggcttccct cctcaaagtt gtggatatgc aactgtgacg gatgccgaag
     1501 cagtgattgt ccaggtgact cctcaccatg tgctggttga tgaatacaca ggagaatggg
     1561 ttgattcaca gttcatcaac ggaaaatgca gcaattacat atgccccact gtccataact
     1621 ctacaacctg gcattctgac tataaggtca aagggctatg tgattctaac ctcatttcca
     1681 tggacatcac cttcttctca gaggacggag agctatcatc cctgggaaag gagggcacag
     1741 ggttcagaag taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa atgcaatact
     1801 gcaagcattg gggagtcaga ctcccatcag gtgtctggtt cgagatggct gataaggatc
     1861 tctttgctgc agccagattc cctgaatgcc cagaagggtc aagtatctct gctccatctc
     1921 agacctcagt ggatgtaagt ctaattcagg acgttgagag gatcttggat tattccctct
     1981 gccaagaaac ctggagcaaa atcagagcgg gtcttccaat ctctccagtg gatctcagct
     2041 atcttgctcc taaaaaccca ggaaccggtc ctgctttcac cataatcaat ggtaccctaa
     2101 aatactttga gaccagatac atcagagtcg atattgctgc tccaatcctc tcaagaatgg
     2161 tcggaatgat cagtggaact accacagaaa gggaactgtg ggatgactgg gcaccatatg
     2221 aagacgtgga aattggaccc aatggagttc tgaggaccag ttcaggatat aagtttcctt
     2281 tatacatgat tggacatggt atgttggact ccgatcttca tcttagctca aaggctcagg
     2341 tgttcgaaca tcctcacatt caagacgctg cttcgcaact tcctgatgat gagagtttat
     2401 tttttggtga tactgggcta tccaaaaatc caatcgagct tgtagaaggt tggttcagta
     2461 gttggaaaag ctctattgcc tcttttttct ttatcatagg gttaatcatt ggactattct
     2521 tggttctccg agttggtatc catctttgca ttaaattaaa gcacaccaag aaaagacaga
     2581 tttatacaga catagagatg aaccgacttg gaaagtaact caaatcctgc acaacagatt
     2641 cttcatgttt ggaccaaatc aacttgtgat accatgctca aagaggcctc aattatattt
     2701 gagtttttaa tttttatgaa aaaaaaaaaa aaaaacggaa ttcctcgagg gatccgtcga
     2761 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2821 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2881 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2941 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     3001 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     3061 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3121 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3181 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3241 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacgg
     3301 atcggccgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3481 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3541 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5461 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     5521 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5581 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5641 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5701 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5761 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5821 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5881 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     5941 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     6001 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6061 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     6121 attggagctc caccgcggtg gcggccgctc taga
//
