LOCUS       pAAV-WRS(hit14)-4xWtR-I 8373 bp ds-DNA     circular     09-JAN-2022
DEFINITION  .
KEYWORDS    "marker:AAV2 ITR, AAV2 ITR, AmpR"
COMMENT     AAV expression of firefly luciferase from CMV promoter . <small>[1]
            AAV plasmids</small>
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    150..812
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    820..1312
                     /label="Chicken beta globin intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1335..2075
                     /label="GFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1335..2075
                     /label="Translation 1335-2075"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKYSDLELK*"
     misc_feature    2108..2586
                     /label="Poly-A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2626..2766
                     /label="ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2788..3047
                     /label="U6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3049..3124
                     /label="EcWtR-TGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3136..3395
                     /label="U6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3397..3472
                     /label="EcWtR-TGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3484..3743
                     /label="U6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3745..3820
                     /label="WtR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3828..4091
                     /label="U6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4093..4168
                     /label="WtR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4180..4670
                     /label="CMV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4715..4731
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4769..4777
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4778..5783
                     /label="WRS (hit 14)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(5853..6077)
                     /label="BgH polyA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      6100..6555
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        6581..6685
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             6686..6754
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             6755..7546
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      7717..8305
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGGAGC TAGTTATTAA TAGTAATCAA
      181 TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA
      241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG
      301 TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT
      361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG
      421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC
      481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC
      541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA
      601 TTGACGTCAA TGGGAGTTTG TTTTGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA
      661 CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
      721 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT
      781 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT
      841 GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA
      901 GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC
      961 TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC
     1021 ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA
     1081 AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA
     1141 CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT
     1201 CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG
     1261 GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT
     1321 CGATTGAATT CTGAATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
     1381 TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
     1441 GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG
     1501 TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC
     1561 CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG
     1621 AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG
     1681 AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA
     1741 ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG
     1801 ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA
     1861 GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC
     1921 TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC
     1981 GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG
     2041 AGCTGTACAA GTACTCAGAT CTCGAGCTCA AGTAAGCTTG CCTCGAGCAG CGCTGCTCGA
     2101 GAGATCTACG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT
     2161 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC
     2221 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT
     2281 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA
     2341 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT
     2401 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG
     2461 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC
     2521 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT
     2581 GTCCTTCTGA TTTTGTAGGT AACCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA
     2641 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG
     2701 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC
     2761 TGCAGGCTCG ATCCGCTCGC ACCCtagGTC GGGCAGGAAG AGGGCCTATT TCCCATGATT
     2821 CCTTCATATT TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTAGAAT TAATTTGACT
     2881 GTAAACACAA AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG
     2941 TTTGCAGTTT TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG
     3001 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCAG GGGCGTAGTT
     3061 CAATTGGTAG AGCACCGGTC TtcAAAACCG GGTGtTGGGA GTTCGAGTCT CTCCGCCCCT
     3121 GCCAtttttt gCTAGGTCGG GCAGGAAGAG GGCCTATTTC CCATGATTCC TTCATATTTG
     3181 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTAGAATTA ATTTGACTGT AAACACAAAG
     3241 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
     3301 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
     3361 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCAGGG GCGTAGTTCA ATTGGTAGAG
     3421 CACCGGTCTt cAAAACCGGG TGtTGGGAGT TCGAGTCTCT CCGCCCCTGC CAttttttgC
     3481 TAGGTCGGGC AGGAAGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC
     3541 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA
     3601 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT
     3661 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT
     3721 ATCTTGTGGA AAGGACGAAA CACCAGGGGC GTAGTTCAAT TGGTAGAGCA CCGGTCTtcA
     3781 AAACCGGGTG tTGGGAGTTC GAGTCTCTCC GCCCCTGCCA ttttttgCTA GGTCGGGCAG
     3841 GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA GGCTGTTAGA
     3901 GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA TACGTGACGT
     3961 AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA AATGGACTAT
     4021 CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA
     4081 GGACGAAACA CCAGGGGCGT AGTTCAATTG GTAGAGCACC GGTCTtcAAA ACCGGGTGtT
     4141 GGGAGTTCGA GTCTCTCCGC CCCTGCCAtt ttttgctagt tattaatagt aatcaattac
     4201 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4261 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4321 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
     4381 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4441 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4501 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4561 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4621 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     4681 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     4741 aagctggcta gCGTTTAAAC TTAAGCTTgc cgccaccATG ACTAAGCCCA TCGTTTTTgc
     4801 tGGCGCACAG CCCTCAGGTG AATTGACCAT TGGTAACTAC ATGGGTGCGC TGCGTCAGTG
     4861 GGTAAACATG CAGGATGACT ACCATTGCAT TTACTGTATC GTTGACCAAC ACGCGATCAC
     4921 CGTGCGCCAG GATGCACAGA AGCTGCGTAA AGCGACGCTG GATACGCTGG CCTTGTATCT
     4981 GGCTTGTGGT ATCGATCCTG AGAAAAGCAC CATTTTTGTT CAGTCCCACG TGCCGGAACA
     5041 TGCACAGTTA GGCTGGGCAC TGAACTGCTA TACCTACTTC GGCGAACTGA GTCGCATGAC
     5101 GCAGTTTAAA GATAAATCTG CGCGTTATGC CGAGAACATC AACGCTGGTC TGTTTGACTA
     5161 TCCGGTGCTG ATGGCAGCGG ACATCCTGCT GTATCAAACT AATCTGggtc cttgtGGTGA
     5221 AGACCAGAAA CAGCACCTCG AACTGAGCCG CGATATTGCC CAGCGTTTCA ACGCGCTGTA
     5281 TGGCGAGATC TTTAAGGTGC CGGAGCCGTT TATTCCGAAA TCTGGCGCGC GCGTAATGTC
     5341 GCTGCTGGAG CCGACCAAGA AGATGTCCAA GTCTGACGAT AATCGCAATA ACGTTATCGG
     5401 CCTGCTGGAA GATCCGAAAT CGGTAGTGAA GAAAATCAAA CGTGCGGTCA CTGACTCCGA
     5461 CGAGCCGCCG GTAGTTCGCT ACGATGTGCA GAACAAAGCG GGCGTTTCCA ACCTGTTGGA
     5521 TATCCTTTCA GCGGTAACGG GCCAGAGCAT CCCAGAACTG GAAAAACAGT TCGAAGGCAA
     5581 GATGTATGGT CATCTGAAAG GTGAAGTGGC TGATGCCGTT TCCGGTATGC TGACTGAATT
     5641 GCAGGAACGC TATCACCGTT TCCGCAACGA TGAAGCCTTC CTGCAACAGG TGATGAAAGA
     5701 TGGCGCGGAA AAAGCCAGCG CGCACGCTTC CCGTACGCTA AAAGCGGTGT ACGAAGCGAT
     5761 TGGTTTTGTG GCGAAGCCGT AAgaATTCAA CGCGTTAAGT CGACtttaac tcgagtctag
     5821 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5881 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5941 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6001 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6061 tgcggtgggc tctatggTAC CttaattAAG CTTGGTACCa cgcgccctgt agcggcgcat
     6121 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6181 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6241 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6301 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6361 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6421 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6481 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6541 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6661 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6721 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6781 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6841 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6901 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6961 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7021 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7081 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7141 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7201 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7261 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7321 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7381 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7441 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7501 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7561 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7621 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7681 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7741 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7801 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7861 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7921 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7981 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8041 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8101 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8161 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8221 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8281 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8341 cttttgctgg ccttttgctc acatgtGCGG CCG
//