LOCUS       pHnS_2comp_KI;Empty        6158 bp    DNA     circular UNA 11-JUL-2025
DEFINITION  .
ACCESSION   urn.local...1a-jebled8
VERSION     urn.local...1a-jebled8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..126
                     /standard_name="ITR"
     source          165..1048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    165..184
                     /standard_name="Primer site #2"
     regulatory      185..497
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/3366121""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/3366121"">Das
                     et al., 1988</a>)"
                     /standard_name="mU6 promoter"
     primer_bind     416..436
                     /standard_name="mU6-F"
     primer_bind     complement(477..498)
                     /standard_name="SEQ_mU6_last_R"
     misc_feature    499..518
                     /locus_tag="DRSR2(1)"
                     /standard_name="DRSR2"
     misc_feature    519..614
                     /standard_name="Scaffold cr2"
     misc_feature    581..592
                     /standard_name="T-lock"
     misc_feature    615..621
                     /standard_name="polyT"
     misc_feature    622..640
                     /standard_name="Primer site #3"
     misc_feature    657..897
                     /standard_name="hU6"
     primer_bind     657..677
                     /standard_name="hU6-F"
     primer_bind     828..847
                     /standard_name="LKO.1 5'"
     misc_feature    907..923
                     /standard_name="BbsI insertion site"
     misc_feature    924..940
                     /standard_name="Scaffold cr3"
     misc_feature    945..1019
                     /standard_name="Scaffold cr3"
     misc_feature    986..997
                     /standard_name="T-lock"
     misc_feature    1020..1026
                     /standard_name="polyT"
     misc_feature    1027..1048
                     /standard_name="Primer site #4"
     regulatory      1080..1527
                     /note="Geneious type: promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/12595892""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/12595892"">Kügl
                     er et al., 2003</a>)"
                     /standard_name="hSyn promoter"
     primer_bind     complement(1081..1104)
                     /standard_name="SEQ_hSyn_0R"
     CDS             1557..2625
                     /codon_start=1
                     /locus_tag="smFP HA"
                     /transl_table=1
                     /translation="MYPYDVPDYAGYPYDVPDYAGYPYDVPDYAGGVSKGEELFTGVV
                     PILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGGGVQC
                     FSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGI
                     DFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEGGYPYDVPDYAGGY
                     PYDVPDYAGGGGYPYDVPDYAGGYPYDVPDYAGGDGSVQLADHYQQNTPIGDGPVLLP
                     DNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGYPYDVPDYAGYPYD
                     VPDYAGYPYDVPDYA*TGARGT"
                     /standard_name="smFP HA"
     CDS             1560..1586
                     /codon_start=1
                     /locus_tag="HA(5)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             1590..1616
                     /codon_start=1
                     /locus_tag="HA(6)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             1620..1646
                     /codon_start=1
                     /locus_tag="HA(7)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2175..2201
                     /codon_start=1
                     /locus_tag="HA(8)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2208..2234
                     /codon_start=1
                     /locus_tag="HA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2247..2273
                     /codon_start=1
                     /locus_tag="HA(1)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2280..2306
                     /codon_start=1
                     /locus_tag="HA(2)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2517..2543
                     /codon_start=1
                     /locus_tag="HA(3)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2547..2573
                     /codon_start=1
                     /locus_tag="HA(4)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     CDS             2577..2603
                     /codon_start=1
                     /locus_tag="HA(9)"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /standard_name="HA"
     misc_feature    2638..2652
                     /locus_tag="X"
                     /standard_name="X"
     misc_feature    2667..3255
                     /locus_tag="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /standard_name="WPRE"
     primer_bind     complement(2720..2740)
                     /locus_tag="WPRE-R"
                     /note="WPRE, reverse primer"
                     /standard_name="WPRE-R"
     CDS             complement(3138..3149)
                     /codon_start=1
                     /locus_tag="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /transl_table=1
                     /translation="IEGR"
                     /standard_name="Factor Xa site"
     primer_bind     complement(3258..3275)
                     /locus_tag="BGH-rev"
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
                     /standard_name="BGH-rev"
     misc_feature    3264..3471
                     /note="Geneious type: polyA_signal"
                     /locus_tag="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /standard_name="bGH poly(A) signal"
     misc_feature    3514..3643
                     /standard_name="ITR"
     rep_origin      complement(3879..4467)
     CDS             join(complement(5430..5498),complement(4638..5429))
                     /codon_start=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /standard_name="AmpR"
     regulatory      complement(5499..5603)
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5629..6084)
ORIGIN      
        1 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt
       61 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      121 ggttccttgt agttaatgat taacccgcca tgctacttat ctacccagtc tgactatcac
      181 gatggatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
      241 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
      301 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
      361 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
      421 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
      481 tggagaaaag ccttgttggc gatcgtaatc acccgagtgt ttgagagcta tgcagaaagc
      541 tgcatagcaa gttcaaataa ggctagtccg tacacaactt gggcttcggc ctaagtggca
      601 gccgagtcgg ctgctttttt tgctcgaatc tacactcagc gcggccgctc acatgtgagg
      661 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      721 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      781 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      841 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      901 acaccgggtc ttcgagaaga cctgtttcag agctatgcac aagagtgcat agcaagttga
      961 aataaggcta gtccgtttac aacttggagc ttgctccaag tggcacccga gtcgggtgct
     1021 ttttttctcg agtactagga tccattaggc ggtctagact gcagagggcc ctgcgtatga
     1081 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     1141 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     1201 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     1261 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     1321 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     1381 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     1441 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     1501 ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc ggatccggcc gccaccatgt
     1561 acccttatga tgtgcccgat tatgctggct acccttatga tgttcctgat tacgccggat
     1621 atccgtatga cgtgccagac tacgcgggag gtgtgagcaa gggagaggag ttgtttaccg
     1681 gcgtggtccc catactggtg gagttggatg gcgacgttaa tggacataaa ttctcggtcc
     1741 gcggcgaggg agagggagac gccaccaatg gcaagctgac gcttaagttc atttgcacga
     1801 ctggaaaatt gcctgtcccc tggccgaccc tggtcaccac actgggcgga ggagtgcagt
     1861 gcttctcccg ttacccagac cacatgaagc agcacgattt cttcaagagc gcaatgccgg
     1921 agggatacgt gcaagaacgg actatttcct tcaaggatga cggaacctat aagacccgtg
     1981 cggaggtcaa attcgagggt gacaccctgg tgaaccgaat tgaactcaaa ggaatcgatt
     2041 tcaaggagga tggaaatatt ctgggtcaca agctggaata caacttcaac agccataatg
     2101 tgtacattac ggctgataag cagaagaacg gcattaaggc caatttcaag atccgccaca
     2161 acgttgaggg tggatatccc tacgacgtgc ccgattatgc gggcggatat ccgtatgatg
     2221 ttccagatta tgctggtggt ggaggctatc cctatgatgt ccccgactac gccggaggtt
     2281 acccatacga cgtgcccgac tacgctggag gcgatggcag cgtgcagctg gcagatcatt
     2341 atcaacagaa tacccccata ggtgatggcc ccgttctgct tccagataat cactaccttt
     2401 ccacccagag cgtgctttcg aaagacccga acgaaaagcg tgatcacatg gtcctgctgg
     2461 agtttgtgac cgcggctgga atcaccctgg gtatggacga actctacaag ggtggctacc
     2521 cctacgatgt gccggattac gctggatatc cgtatgacgt accggactat gccggttacc
     2581 cgtacgatgt cccggactac gcttaaaccg gtgctcgagg taccgaattc gatatcatag
     2641 ctagctagct agggatcggt gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     2701 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2761 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2821 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2881 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2941 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3001 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3061 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3121 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3181 cggctctgcg gcctcttccg cgtctacgcc ttcgccctca gacgagtcgg atctcccttt
     3241 gggccgcctc cccgcctcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3421 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcgggtaga
     3481 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     3541 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3601 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     3661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     5641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6061 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     6121 ggcgatcggt gcgggcctct tcgctattac gccagctg
//
