LOCUS       pUC19-U6_AluJb          3169 bp    DNA     circular SYN 21-MAY-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   JB
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             111..127
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMIT"
     primer_bind     128..156
                     /label=TAAGCA_HindIII_U6_For
     promoter        133..373
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(350..385)
                     /label=AluJB_exten_U6_R
     primer_bind     350..385
                     /label=U6_exten_AluJB_F
     misc_feature    374..656
                     /label=AluJb_Repbase
     misc_feature    386..396
                     /label=A_box
     misc_feature    450..458
                     /label=B_box
     primer_bind     470..489
                     /label=AluJB_qPCR3_F
     misc_feature    491..506
                     /label=A-rich linker
     primer_bind     500..519
                     /label=AluJB_qPCR4_F
     primer_bind     complement(636..667)
                     /label=TAAGCA_EcoRI_5T_AluJB_R
     terminator      657..661
     CDS             663..917
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="NSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     misc_feature    663..667
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(666..685)
                     /label=AluJB_qPCR3_R
     primer_bind     complement(668..684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(689..708)
                     /label=AluJB_qPCR4_R
     primer_bind     complement(893..912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     1012..1034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1072..1090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        1158..1262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1263..1331,1332..2123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1481..1500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3036..3053
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccagc ttgagggcct atttcccatg attccttcat atttgcatat acgatacaag
      181 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
      241 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      301 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      361 ttgtggaaag gacggccggg cgcggtggct cacgcctgta atcccagcac tttgggaggc
      421 cgaggcggga ggatcacttg agcccaggag ttcgagacca gcctgggcaa catggtgaaa
      481 ccccgtctct acaaaaaata caaaaattag ccgggcgtgg tggcgcgcgc ctgtagtccc
      541 agctactcgg gaggctgagg caggaggatc gcttgagccc gggaggtcga ggctgcagtg
      601 agccgtgatc gcgccactgc actccagcct gggcgacaga gcgagaccct gtctcatttt
      661 tgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      841 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      901 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      961 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1021 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1081 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1261 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1321 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1381 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1441 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1501 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1561 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1621 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1681 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1741 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1801 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1861 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1921 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1981 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2041 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2101 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2161 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2221 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2461 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2941 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3001 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3061 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3121 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaat
//
