LOCUS       pET-15b_PCR_Product_-_pCDFDuet-His6NocM-NocL_PCR_Product        7610 bp    DNA     circular UNA 03-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...ic-i5xa443
VERSION     urn.local...ic-i5xa443
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(complement(1..>5355),complement(<7285..7610))
                     /organism="pET-15b"
                     /mol_type="other DNA"
                     /label="source pET-15b"
     promoter        139..167
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
     gene            209..1069
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="Ampicillin"
     CDS             209..1069
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 2"
     misc_feature    2242..2264
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="pGEX_3_primer"
     misc_feature    complement(2258..2449)
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="ROP"
     gene            complement(3088..3702)
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="tet (576 - 1190)"
     CDS             complement(3088..3705)
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="ORF frame 3"
     CDS             3283..3978
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
                     /label="ORF frame 1"
     CDS             complement(3761..4720)
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="ORF frame 2"
     misc_feature    complement(3761..4852)
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="lacI"
     CDS             complement(4593..5093)
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="ORF frame 1"
     misc_feature    5127..5146
                     /label="pBRrevBam_primer"
     promoter        5230..5248
                     /label="T7_promoter"
     misc_feature    5248..5275
                     /label="lacO"
     misc_feature    5293..5309
                     /label="T7_transl_en_RBS"
     ORF             join(<7285..7293,5317..>5355)
                     /codon_start=1
                     /created_by="nateg"
                     /label
     ligation        5338..5355
                     /label="Ligation"
     misc_feature    5338..7306
                     /Source="referenceToDocument: urn:local:.:hu-i5xa3jn"
                     /label="pCDFDuet-His6NocM-NocL PCR Product (concatenated
                     sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     complement(5338..5355)
                     /%GC="61.1"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="5.6"
                     /Tm="59.3"
                     /annotation_group="62729935288006b10a00b-b194-4b6d-9a13-55
                     071f240fd6"
                     /created_by="primer3"
                     /Sequence="GCCGCTGCTGTGATGATG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pET-15b"
                     /label="pET15-His6-r"
     ORF             5356..7284
                     /created_by="nateg"
                     /modified_by="nateg"
                     /label
     source          <5356..>7281
                     /organism="Nodularia sp. LEGE 06071 N071000019"
                     /label="source Nodularia sp. LEGE 06071 N071000019"
     gene            5356..7281
                     /product="Long-chain-fatty-acid--(Acyl-carrier-protein)
                     lig ase, 6-deoxyerythronolide-B synthase (EC 2.3.1.94) (EC
                     6.2. 1.20)"
                     /modified_by="nateg"
                     /label="NocL (fatty acyl-AMP ligase)"
     CDS             5356..7281
                     /product="Long-chain-fatty-acid--(Acyl-carrier-protein)
                     lig ase, 6-deoxyerythronolide-B synthase (EC 2.3.1.94) (EC
                     6.2. 1.20)"
                     /translation="MNVRLTDSPNPLEKFSTLIELLIYRAQNQPQQSAYTFLRDGETE
                     EINLTYGELDQKVRAIASHLQSLGVSGERALLLYPPGLDFITAFLGCLYAGVVAVPAY
                     PPRKNMNFLRLQAIATDAQATVVLTTSDLLANLVSQWAENPELPTMHWLSTDDIDSNL
                     SSDWQAPRLERDTLAFLQYTSGSTGTPKGVMVTHGNLLSNQRAIKVGFGHTEKTIFVG
                     WLPLFHDMGLIGNVLQPLYLGIPSILMSPVAFLQKPLRWLQAISRYKATTSGGPNFAY
                     DLCIRKITPEELSRLDLSSWEVAFNGAEPVRSETLERFANYFAECGFRKEAFYPCYGM
                     AETTLFVSGGLKTSPPVLYELEAAALEENQVVAAESDEGTRTIVGCGRTWLDEKIAIV
                     DPESLTLCPPSKVGEIWVSGSSVAAGYWRRPEQTTETFQAYLSDKDVSDRDEASRREA
                     LGASGPFLRTGDLGFLQDEELFVTGRLKDVIIVRGQNHYPQDIELTVQNSHPALRLNS
                     GAAFTVEQKGQQRLVIVQEVERTSLRRLDIKEVVGNITEAVIDHHGLQVYATVLIKPG
                     SIPKTSSGKIQRHACRRGFLDGSLNVLENSSRSPHNQDTFPQLADDVNSLLTKVQEVN
                     PDLKKANIKEGEKANALQ"
                     /label="Long-chain-fatty-acid--(Acyl-carrier-protein) lig
                     ase, 6-deoxyerythronolide-B synthase (EC 2.3.1.94) (EC
                     6.2. 1.20) CDS"
     misc_feature    <5356..>7284
                     /Source="referenceToDocument: urn:local:.:id-i5xa443"
                     /label="NodCylA for Gibson (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     5356..5377
                     /created_by="michellewang"
                     /Sequence="ATGAATGTTCGTTTGACCGATT"
                     /Tm_(binding_region)="57.0"
                     /Tm_(with_extension)="67.5"
                     /%GC_(binding_region)="36.4"
                     /%GC_(with_extension)="47.5"
                     /Hairpin_Tm_(with_extension)="42.2"
                     /Self_Dimer_Tm_(with_extension)="12.5"
                     /Extension="CATCATCACAGCAGCGGC"
                     /modified_by="michellewang"
                     /label="pET-15b-NocL-fwd"
     primer_bind     5356..5374
                     /Mismatches=0
                     /Extension="GTATATTAGTTAAGTATAAGAAGGAGATATACC"
                     /%GC="42.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Tm="54.0"
                     /annotation_group="148316104394178bb56e57b-dbb7-4d7c-bf43-
                     1726f25263f0"
                     /Sequence="ATGAATGTTCGTTTGACCG"
                     /created_by="primer3"
                     /Product_Size=1926
                     /label="MCS2-NodCylA-f"
     misc_feature    complement(7259..7281)
                     /Mismatches=0
                     /Extension="GTAGACGAGTCCATGTGCTTATTA"
                     /%GC="34.8"
                     /Hairpin_Tm="42.7"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Tm="56.5"
                     /annotation_group="148316104394178bb56e57b-dbb7-4d7c-bf43-
                     1726f25263f0"
                     /Sequence="TTGAAGTGCATTAGCTTTTTCAC"
                     /created_by="primer3"
                     /Product_Size=1926
                     /label="NodCylA-MCS2-r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(7259..7284)
                     /created_by="michellewang"
                     /Sequence="TTATTGAAGTGCATTAGCTTTTTCAC"
                     /Tm_(binding_region)="57.5"
                     /Tm_(with_extension)="66.1"
                     /%GC_(binding_region)="30.8"
                     /%GC_(with_extension)="41.7"
                     /Hairpin_Tm_(with_extension)="40.6"
                     /Self_Dimer_Tm_(with_extension)="2.6"
                     /Extension="CTTTGTTAGCAGCCGGATCCTC"
                     /modified_by="michellewang"
                     /label="pET15b-NocL-rev"
     gene            7271..>7281
                     /created_by="nateg"
                     /modified_by="nateg"
                     /label="acyl-CoA dehydrogenase"
     misc_feature    7282..>7284
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(7282..>7284)
                     /label="Stop"
     ligation        7285..7306
                     /label="Ligation"
     misc_feature    7285..5355
                     /Source="referenceToDocument: urn:local:.:cv-i5x9h0j"
                     /label="pET-15b PCR Product (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     7285..7306
                     /#_Off-target_Sites=0
                     /Off-target_Template="pET-15b"
                     /Sequence="GAGGATCCGGCTGCTAACAAAG"
                     /Tm="61.3"
                     /%GC="54.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /modified_by="michellewang"
                     /label="pET15-f"
     terminator      7295..7423
                     /label="T7_terminator"
     misc_feature    complement(7337..7355)
                     /label="T7_Terminal_primer"
ORIGIN      
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
     5341 catcacagca gcggcatgaa tgttcgtttg accgattcgc caaatcctct agagaaattt
     5401 tcaacattaa tagagttact gatctataga gcgcagaatc aaccacagca gagtgcttac
     5461 acatttctgc gcgatggaga aactgaagaa atcaacctga cttatggaga actagaccaa
     5521 aaagttaggg cgatcgcatc tcatcttcag tctctaggag tatctggcga acgtgcgtta
     5581 ctgttgtatc caccaggtct agatttcatt actgccttct tggggtgttt atatgcaggt
     5641 gttgtagcag ttccagcata tccacccaga aagaatatga acttcttgag gttgcaggcg
     5701 atcgccacag atgctcaagc aacggtagta ttgactactt cagacttgtt agccaacttg
     5761 gtcagtcaat gggctgaaaa tccagaattg ccaactatgc actggttaag cacagatgac
     5821 atcgacagta atctcagttc agattggcaa gcaccaagac tagagaggga taccctggcg
     5881 tttctccaat atacttctgg ctcgacagga acgccaaagg gagtgatggt aacacatggg
     5941 aatttgctat ccaaccagag agcgatcaag gtcggcttcg gacatacaga gaaaacaatt
     6001 ttcgtcggtt ggttgccctt atttcacgat atgggattga ttggcaatgt cttgcagccc
     6061 ctatatttgg gtataccttc cattctcatg tcaccagtag cttttctgca aaagcctttg
     6121 cgttggttgc aagctatttc tcgttacaaa gccactacta gtggtgggcc aaattttgcc
     6181 tatgatcttt gcatccgcaa aattactcct gaagaacttt ctcgtcttga cttgagcagt
     6241 tgggaagttg ctttcaacgg agccgaacca gtccggtctg aaacattaga gcgctttgct
     6301 aactattttg ccgagtgtgg tttccgtaaa gaggcatttt atccctgcta cggtatggct
     6361 gaaacgactt tattcgtgtc cggaggtttg aaaacatctc cccccgttct gtatgagcta
     6421 gaagcagcag cccttgagga aaatcaggta gtggcggctg aatctgacga gggaactaga
     6481 acaattgttg gctgtggccg aacctggtta gatgagaaaa tcgctattgt cgatcccgaa
     6541 tccttaactc tgtgtccccc cagtaaagtg ggagagattt gggtgtcggg ttcttctgtg
     6601 gctgctggct actggagacg accagagcaa acaaccgaaa cctttcaagc ttatctgtca
     6661 gacaaagatg ttagcgatcg cgacgaagca tctcgtagag aagcgttagg agcgtcaggc
     6721 ccgtttctcc gaaccggaga cttaggcttt ttgcaagacg aagagttgtt tgttacagga
     6781 agactcaagg atgtgattat tgtccgcgga caaaatcatt atccccagga tattgagtta
     6841 acggttcaaa acagtcatcc agcactgcga ttaaatagtg gggcagcctt tacagtagag
     6901 caaaaagggc aacagcgatt agttattgtt caagaggtgg aacgaacctc tttgaggcgg
     6961 ttggatataa aagaggtcgt cggcaatatc accgaagcag tgatagatca ccacggctta
     7021 caggtgtatg ccacagtttt aattaagcct gggagtatcc ccaagacctc tagtgggaaa
     7081 attcagcgcc atgcttgtcg gcgtgggttt ctagatggca gtttgaatgt gctggagaac
     7141 tctagtagaa gtcctcataa ccaagataca tttccacagt tggcagatga tgttaactcc
     7201 ctcctcacaa aagtgcagga agttaatcct gatttgaaaa aagcgaatat caaggagggt
     7261 gaaaaagcta atgcacttca ataagaggat ccggctgcta acaaagcccg aaaggaagct
     7321 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     7381 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatcccgc aagaggcccg
     7441 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     7501 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
     7561 ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
//
