LOCUS       pAM_048_pMi{3xp3::mCherry-SV40,FibL::mBaojin-P10UTR}        7403 bp    DNA     circular UNA 25-MAY-2025
DEFINITION  len=7403.
ACCESSION   urn.local...10-j2ge25o
VERSION     urn.local...10-j2ge25o
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    0^1
                     /Original_Bases="CACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAA
                     GCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCAC
                     AACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCG
                     GCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGGCCGCGACTCTAGATCATAATCAG
                     CCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTG
                     AACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATA
                     ATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACT
                     GCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAGCTAGCTATTGAATGC
                     AATAAGATGTCACAATACAATAAAAGCTTGTGTCAAAAATGGTATATTTATTAAAAAA
                     ATTATTCTCTTAAGACTGCTATCCATCATCTAAATATACCTATAAATTAATCCGAGTA
                     AAACATCTATACTTATCTAATTTTTATTGAAAATCTTTACGATACAATAGAAGACTGA
                     ATTTGACTTTGTCTTATCCGGCTTTGTTTTGTAATTTAGAGATTTTATGCGATTTCAG
                     CGAAGATACGGGTGTAATTCGCAACAAAGATGGTAATATATCGTAAAAATGCTTAGCC
                     TTTTATGATTGAGTTGACGGTGCTCGTTCGCCGACTTTGTATGTTTGGGGAAAAGATA
                     AAGGTAAACAATTACCAGTGTCCACAAGTGATTGACAAGTCTCTCATCCTCGCGTGTT
                     CTCGATTAAATTTGGTGCTGGACGCCCCGAAGTCCAAAGATCGTGTAAAACAAGGAAC
                     ATTTAAATTTTGAAGGTTTGGCCGTGCTGATGTTTAGTTAGTCCGAGTTGCTCTTCTA
                     ACCTATTGACGTGTCTAATTGACGTGTTAGCAAATCCTTCATTTTTAACCTTCAACTT
                     TATTTACTTAACAAATGACGTTGCAAGTGAAAAATATCTTAATTTCACATTGTACCGA
                     AAACAATTTCCATTAATGCTCGCCTTTGGACAAACATATAAACAAACGATTCAATTAA
                     TCGCTTTGTACAAAATCTAAACTAAAACAATTATATACCTGCATTTATATAACAATCA
                     TCAATTCACTTTAAACCTTAATGCAACAGTAATAAGATTCGCACTTAAAAATTTATGA
                     AATAATGTCGTTTCGTTTTCTTGTTAATCTGGTTATTGTTCATAGGGTGTTATTTGAC
                     CAGGTGCAAATTGTTTGAAGATTATTTATATATATTGTTATATTAGTGTATAAATAGT
                     AGATAAAGACAGACGATTTTGTGTATAATATATACCGAATTGGTCGAATTAAGACCAG
                     CAAATAGTAGTATCAAAATGGTCTCAAAGGGAGAGGAGGAAAACATGGCTAGTACACC
                     ATTTAAATTTCAACTTAAAGGAACCATCAATGGCAAATCGTTTACCGTTGAAGGCGAA
                     GGTGAAGGGAACTCACATGAAGGTTCTCATAAAGGAAAATATGTTTGTACAAGTGGAA
                     AACTACCGATGTCATGGGCAGCACTTGGGACAACCTTTGGTTATGGAATGAAATATTA
                     TACCAAATATCCTAGTGGACTGAAGAACTGGTTTCGTGAAGTAATGCCCGGAGGCTTT
                     ACCTACGATCGTCATATTCAATATAAAGGCGATGGGAGTATCCATGCAAAACACCAAC
                     ACTTTATGAAAAATGGGACTTATCACAACATTGTAGAATTTACCGGTCAGGATTTTAA
                     AGAAAATAGTCCAGTCTTAACTGGAGATATGAATGTCTCATTACCGAATGAAGTCCCA
                     CAAATACCCAGAGATGATGGAGTAGAATGCCCAGTGACCTTGCTTTATCCTTTATTAT
                     CGGATAAATCAAAATACGTCGAGGCTCACCAATATACAATCTGCAAGCCTCTTCATAA
                     TCAACCAGCACCTGATGTCCCATATCACTGGATTCGTAAACAATACACACAAAGCAAA
                     GATGATGCCGAGGAACGCGATCATATCTGTCAATCAGAGACTCTCGAAGCACACTTAA
                     AGGGCATGGACGAGCTGTATAAGTAGCACGTGATGAATCGTTTTTAAAATAACAAATC
                     AATTGTTTTATAATATTCGTACGATTCTTTGATTATGTAATAAAATGTGATCATTAGG
                     AAGATTACGAAAAATATAAAAAATATGAGTTCTGTGTGTATAACAAATGCTGTAAACG
                     CCACAATTGTGTTTGTTGCAAATAAACCCATGATTATTTGATTAAAATTGTTGTTTTC
                     TTTGTTCATAGACAATAGTGTGTTTTGCCTAAACGTGTACTGCATAAACTCCATGCGA
                     GTGTATAGCGAGCTAGTGGCTAACGCTTGCCCCACCAAAGTAGATTCGTCAAAATCCT
                     CAATTTCATCACCCTCCTCCAAGTTTAACATTTGGCCGTCGGAATTAACTTCTAAAGA
                     TGCCACATAATCTAATAAATGAAATAGAGATTCAAACGTGGCGTCATCGTCCGTTTCG
                     ACCATTTCCGAAAAGAACTCGGGCATAAACTCTATGATTTCTCTGGACGTGGTGTTGT
                     CGAAACTCTCAAAGTACGCAGTCAGGAACGTGCGCGACATGTCGTCGGGAAACTCGCG
                     CGGAAACATGTTGTTGTAACCGAACGGGTCCCATAGCGCCAAAACCAAATCTGCCAGC
                     GTCAATAGAATGAGCACGATGCCGACAATGGAGCTGGCTTGGATAGCGATTCGAGTTA
                     ACAACGGCCGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGGACGTTACACAATT
                     CTAATATTAATTAAATTATTGTTTTAAGTATGATAGTAAATCACATTACGCCGCGTTC
                     GAATTAATAGTGGTCACTTTTTTCTTATCTCTTAAGCAAACCGTTTGAATAAATTACT
                     CATATTTTTGTTGTTGTTGGAAATAGAGCAAAACTTTTTTTTTCGTCGTGAAGAGAAT
                     AAAATTCTCTTTGAGACGAAATGCATTGGTATGTGTTATCTTTAGTAGTATTGATAAT
                     ATAGTGTGTTAAACATTGCGCACTGCAAAAAAAACATGCTGTTCGAATTAATAGTGGT
                     TGGGGCTCGTAGAAAACGAAAAATATCTTAAGCTAGCATAGAGAATGGAGCAAAACTC
                     AATTTGAT"
                     /label="CACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCA
                     GCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGAC
                     TACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGG
                     ACGAGCTGTACAAGTAAAGCGGCCGGCCGCGACTCTAGATCATAATCAGCCATACCAC
                     ATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAA
                     CATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACA
                     AATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG
                     TTGTGGTTTGTCCAAACTCATCAATGTATCTTAGCTAGCTATTGAATGCAATAAGATG
                     TCACAATACAATAAAAGCTTGTGTCAAAAATGGTATATTTATTAAAAAAATTATTCTC
                     TTAAGACTGCTATCCATCATCTAAATATACCTATAAATTAATCCGAGTAAAACATCTA
                     TACTTATCTAATTTTTATTGAAAATCTTTACGATACAATAGAAGACTGAATTTGACTT
                     TGTCTTATCCGGCTTTGTTTTGTAATTTAGAGATTTTATGCGATTTCAGCGAAGATAC
                     GGGTGTAATTCGCAACAAAGATGGTAATATATCGTAAAAATGCTTAGCCTTTTATGAT
                     TGAGTTGACGGTGCTCGTTCGCCGACTTTGTATGTTTGGGGAAAAGATAAAGGTAAAC
                     AATTACCAGTGTCCACAAGTGATTGACAAGTCTCTCATCCTCGCGTGTTCTCGATTAA
                     ATTTGGTGCTGGACGCCCCGAAGTCCAAAGATCGTGTAAAACAAGGAACATTTAAATT
                     TTGAAGGTTTGGCCGTGCTGATGTTTAGTTAGTCCGAGTTGCTCTTCTAACCTATTGA
                     CGTGTCTAATTGACGTGTTAGCAAATCCTTCATTTTTAACCTTCAACTTTATTTACTT
                     AACAAATGACGTTGCAAGTGAAAAATATCTTAATTTCACATTGTACCGAAAACAATTT
                     CCATTAATGCTCGCCTTTGGACAAACATATAAACAAACGATTCAATTAATCGCTTTGT
                     ACAAAATCTAAACTAAAACAATTATATACCTGCATTTATATAACAATCATCAATTCAC
                     TTTAAACCTTAATGCAACAGTAATAAGATTCGCACTTAAAAATTTATGAAATAATGTC
                     GTTTCGTTTTCTTGTTAATCTGGTTATTGTTCATAGGGTGTTATTTGACCAGGTGCAA
                     ATTGTTTGAAGATTATTTATATATATTGTTATATTAGTGTATAAATAGTAGATAAAGA
                     CAGACGATTTTGTGTATAATATATACCGAATTGGTCGAATTAAGACCAGCAAATAGTA
                     GTATCAAAATGGTCTCAAAGGGAGAGGAGGAAAACATGGCTAGTACACCATTTAAATT
                     TCAACTTAAAGGAACCATCAATGGCAAATCGTTTACCGTTGAAGGCGAAGGTGAAGGG
                     AACTCACATGAAGGTTCTCATAAAGGAAAATATGTTTGTACAAGTGGAAAACTACCGA
                     TGTCATGGGCAGCACTTGGGACAACCTTTGGTTATGGAATGAAATATTATACCAAATA
                     TCCTAGTGGACTGAAGAACTGGTTTCGTGAAGTAATGCCCGGAGGCTTTACCTACGAT
                     CGTCATATTCAATATAAAGGCGATGGGAGTATCCATGCAAAACACCAACACTTTATGA
                     AAAATGGGACTTATCACAACATTGTAGAATTTACCGGTCAGGATTTTAAAGAAAATAG
                     TCCAGTCTTAACTGGAGATATGAATGTCTCATTACCGAATGAAGTCCCACAAATACCC
                     AGAGATGATGGAGTAGAATGCCCAGTGACCTTGCTTTATCCTTTATTATCGGATAAAT
                     CAAAATACGTCGAGGCTCACCAATATACAATCTGCAAGCCTCTTCATAATCAACCAGC
                     ACCTGATGTCCCATATCACTGGATTCGTAAACAATACACACAAAGCAAAGATGATGCC
                     GAGGAACGCGATCATATCTGTCAATCAGAGACTCTCGAAGCACACTTAAAGGGCATGG
                     ACGAGCTGTATAAGTAGCACGTGATGAATCGTTTTTAAAATAACAAATCAATTGTTTT
                     ATAATATTCGTACGATTCTTTGATTATGTAATAAAATGTGATCATTAGGAAGATTACG
                     AAAAATATAAAAAATATGAGTTCTGTGTGTATAACAAATGCTGTAAACGCCACAATTG
                     TGTTTGTTGCAAATAAACCCATGATTATTTGATTAAAATTGTTGTTTTCTTTGTTCAT
                     AGACAATAGTGTGTTTTGCCTAAACGTGTACTGCATAAACTCCATGCGAGTGTATAGC
                     GAGCTAGTGGCTAACGCTTGCCCCACCAAAGTAGATTCGTCAAAATCCTCAATTTCAT
                     CACCCTCCTCCAAGTTTAACATTTGGCCGTCGGAATTAACTTCTAAAGATGCCACATA
                     ATCTAATAAATGAAATAGAGATTCAAACGTGGCGTCATCGTCCGTTTCGACCATTTCC
                     GAAAAGAACTCGGGCATAAACTCTATGATTTCTCTGGACGTGGTGTTGTCGAAACTCT
                     CAAAGTACGCAGTCAGGAACGTGCGCGACATGTCGTCGGGAAACTCGCGCGGAAACAT
                     GTTGTTGTAACCGAACGGGTCCCATAGCGCCAAAACCAAATCTGCCAGCGTCAATAGA
                     ATGAGCACGATGCCGACAATGGAGCTGGCTTGGATAGCGATTCGAGTTAACAACGGCC
                     GGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGGACGTTACACAATTCTAATATTA
                     ATTAAATTATTGTTTTAAGTATGATAGTAAATCACATTACGCCGCGTTCGAATTAATA
                     GTGGTCACTTTTTTCTTATCTCTTAAGCAAACCGTTTGAATAAATTACTCATATTTTT
                     GTTGTTGTTGGAAATAGAGCAAAACTTTTTTTTTCGTCGTGAAGAGAATAAAATTCTC
                     TTTGAGACGAAATGCATTGGTATGTGTTATCTTTAGTAGTATTGATAATATAGTGTGT
                     TAAACATTGCGCACTGCAAAAAAAACATGCTGTTCGAATTAATAGTGGTTGGGGCTCG
                     TAGAAAACGAAAAATATCTTAAGCTAGCATAGAGAATGGAGCAAAACTCAATTTGAT"
                     /note="Geneious type: Editing History Deletion"
     misc            1..199
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     rep_origin      200..655
                     /created_by="Luca Miles"
                     /modified_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rep
                     Origin (<a href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Rep Origin"
     misc            656..680
                     /created_by="Luca Miles"
                     /modified_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     promoter        681..785
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     Promoter (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="AmpR Promoter"
     CDS             786..1646
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="AmpR"
     misc            1647..1816
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     rep_origin      1817..2405
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Rep_Origin (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Rep_Origin"
     misc            2406..2728
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     binding_site    2693..2714
                     /created_by="Luca Miles"
                     /label="CAP Binding Site"
     promoter        2729..2759
                     /created_by="Luca Miles"
                     /modified_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Lac
                     Promoter (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Lac Promoter"
     Operator        2767..2783
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Lac
                     Operator (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Lac Operator"
     primer_bind     2772..2794
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Tm="59.6"
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="Luca Miles"
                     /label="M13/pUC Reverse"
     primer_bind     2791..2807
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Tm="49.1"
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="Luca Miles"
                     /label="M13 Reverse"
     promoter        2828..2846
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     Promoter (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="T3 Promoter"
     repeat          2961..3213
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Minos
                     IR(<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed)</
                     a>)"
                     /label="Minos IR"
     primer_bind     3329..3345
                     /Sequence="TCGAGGTCGACGGTATC"
                     /Tm="54.9"
                     /%GC="58.8"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="9.9"
                     /created_by="Luca Miles"
                     /label="pBluescriptKS"
     promoter        3379..3583
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3xP3
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="3xP3"
     primer_bind     complement(3564..3583)
                     /Sequence="CGATTGTTTAGCTTGTTCAG"
                     /Tm="52.9"
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="Luca Miles"
                     /label="pMI_019_Backbone_Reverse"
     misc            3584..3630
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3xp3
                     Gap(<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed)</
                     a>)"
                     /label="3xp3 Gap"
     regulatory      3631..3641
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kozak
                     Sequence (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Kozak Sequence"
     CDS             3637..4347
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="99.86%"
                     /Primary_Match="99.86%; Adjusted-Interval-0:0/0; mCherry
                     (<a href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="mCherry"
     misc            4348..4473
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     terminator      4474..4595
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="SV40"
     primer_bind     complement(4511..4530)
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /Tm="51.5"
                     /%GC="35.0"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /created_by="Luca Miles"
                     /label="SV40 PA Rev"
     primer_bind     4565..4584
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Tm="54.8"
                     /%GC="45.0"
                     /Hairpin_Tm="48.9"
                     /Self_Dimer_Tm="19.2"
                     /created_by="Luca Miles"
                     /label="EBV Reverse"
     Restriction     4596..4600
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; NheI
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="NheI"
     promoter        4601..5601
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     FibL_Promoter (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="FibL_Promoter"
     Restriction     5602..5614
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BmtI
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="BmtI"
     CDS             5615..6319
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     mStayGold_mBaojin (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /modified_by="arnaudmartin"
                     /label="mBaojin"
     Restriction     6320..6325
                     /created_by="Luca Miles"
                     /modified_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PmlI
                     (<ahref=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed
                     )</a>)"
                     /label="PmlI"
     3'UTR           6326..7000
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     P10_3'UTR (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="P10_3'UTR"
     Primer          7001..7019
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pMi_019_Backbone_Fwd (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="pMi_019_Backbone_Fwd"
     primer_bind     complement(7001..7017)
                     /Sequence="TCTAGAACTAGTGGATC"
                     /Tm="45.1"
                     /%GC="41.2"
                     /Hairpin_Tm="34.8"
                     /Self_Dimer_Tm="None"
                     /created_by="Luca Miles"
                     /label="pBluescriptSK Reverse"
     misc            7020..7092
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
     repeat          7093..7346
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Minos
                     IR(<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1(reversed)</
                     a>)"
                     /label="Minos IR"
     misc            7347..7403
                     /created_by="Luca Miles"
                     /note="Derived using Geneious Prime 2024.0.2 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="FibL-C2.contig1 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Gap (<a
                     href=""urn:local:.:o-j1vaz1l"">FibL-C2.contig1
                     (reversed)</a>)"
                     /label="Gap"
ORIGIN      
        1 gcgagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
       61 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      121 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      181 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      241 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      301 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      361 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      421 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      481 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      541 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      601 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
      661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2461 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2521 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2581 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2641 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2701 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2761 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2821 agctcgaaat taaccctcac taaagggaac aaaagctggt accaagtgct tgaaatgcta
     2881 aatgttttca atttttcgcc attaagacaa gcctacacaa atgcttctat aaattatgcc
     2941 aagcacgtta gcagcttcta cgagccccaa ccactattaa ttcgaacagc atgttttttt
     3001 gcagtgcgca atgtttaaca cactatatta tcaatactac taaagataac acataccaat
     3061 gcatttcgtc tcaaagagaa ttttattctc ttcacgacga aaaaaaaagt tttgctctat
     3121 ttccaacaac aacaaaaata tgagtaattt attcaaacgg tttgcttaag agataagaaa
     3181 aaagtgacca ctattaattc gaacgcggcg taagcttacc ttaatctcaa gaagagcaaa
     3241 acaaaagcaa ctaatgtaac ggaatcatta tctagttatg atctgcaaat aatgctgcag
     3301 agctggggga tccccggcgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     3361 gaattcgagc tcgcccgggg atctaattca attagagact aattcaatta gagctaattc
     3421 aattaggatc caagcttatc gatttcgaac cctcgaccgc cggagtataa atagaggcgc
     3481 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
     3541 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcggggtacc gctagagtcg
     3601 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggaggat
     3661 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3781 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3841 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3901 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3961 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     4021 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4081 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4141 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     4201 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     4261 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     4321 ggcggcatgg acgagctgta caagtaaagc ggccggccgc gactctagat cataatcagc
     4381 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4441 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4561 agttgtggtt tgtccaaact catcaatgta tcttagctag ctattgaatg caataagatg
     4621 tcacaataca ataaaagctt gtgtcaaaaa tggtatattt attaaaaaaa ttattctctt
     4681 aagactgcta tccatcatct aaatatacct ataaattaat ccgagtaaaa catctatact
     4741 tatctaattt ttattgaaaa tctttacgat acaatagaag actgaatttg actttgtctt
     4801 atccggcttt gttttgtaat ttagagattt tatgcgattt cagcgaagat acgggtgtaa
     4861 ttcgcaacaa agatggtaat atatcgtaaa aatgcttagc cttttatgat tgagttgacg
     4921 gtgctcgttc gccgactttg tatgtttggg gaaaagataa aggtaaacaa ttaccagtgt
     4981 ccacaagtga ttgacaagtc tctcatcctc gcgtgttctc gattaaattt ggtgctggac
     5041 gccccgaagt ccaaagatcg tgtaaaacaa ggaacattta aattttgaag gtttggccgt
     5101 gctgatgttt agttagtccg agttgctctt ctaacctatt gacgtgtcta attgacgtgt
     5161 tagcaaatcc ttcattttta accttcaact ttatttactt aacaaatgac gttgcaagtg
     5221 aaaaatatct taatttcaca ttgtaccgaa aacaatttcc attaatgctc gcctttggac
     5281 aaacatataa acaaacgatt caattaatcg ctttgtacaa aatctaaact aaaacaatta
     5341 tatacctgca tttatataac aatcatcaat tcactttaaa ccttaatgca acagtaataa
     5401 gattcgcact taaaaattta tgaaataatg tcgtttcgtt ttcttgttaa tctggttatt
     5461 gttcataggg tgttatttga ccaggtgcaa attgtttgaa gattatttat atatattgtt
     5521 atattagtgt ataaatagta gataaagaca gacgattttg tgtataatat ataccgaatt
     5581 ggtcgaatta agaccagcaa atagtagtat caaaatggtc tcaaagggag aggaggaaaa
     5641 catggctagt acaccattta aatttcaact taaaggaacc atcaatggca aatcgtttac
     5701 cgttgaaggc gaaggtgaag ggaactcaca tgaaggttct cataaaggaa aatatgtttg
     5761 tacaagtgga aaactaccga tgtcatgggc agcacttggg acaacctttg gttatggaat
     5821 gaaatattat accaaatatc ctagtggact gaagaactgg tttcgtgaag taatgcccgg
     5881 aggctttacc tacgatcgtc atattcaata taaaggcgat gggagtatcc atgcaaaaca
     5941 ccaacacttt atgaaaaatg ggacttatca caacattgta gaatttaccg gtcaggattt
     6001 taaagaaaat agtccagtct taactggaga tatgaatgtc tcattaccga atgaagtccc
     6061 acaaataccc agagatgatg gagtagaatg cccagtgacc ttgctttatc ctttattatc
     6121 ggataaatca aaatacgtcg aggctcacca atatacaatc tgcaagcctc ttcataatca
     6181 accagcacct gatgtcccat atcactggat tcgtaaacaa tacacacaaa gcaaagatga
     6241 tgccgaggaa cgcgatcata tctgtcaatc agagactctc gaagcacact taaagggcat
     6301 ggacgagctg tataagtagc acgtgatgaa tcgtttttaa aataacaaat caattgtttt
     6361 ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga agattacgaa
     6421 aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt
     6481 tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt tcatagacaa
     6541 tagtgtgttt tgcctaaacg tgtactgcat aaactccatg cgagtgtata gcgagctagt
     6601 ggctaacgct tgccccacca aagtagattc gtcaaaatcc tcaatttcat caccctcctc
     6661 caagtttaac atttggccgt cggaattaac ttctaaagat gccacataat ctaataaatg
     6721 aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg
     6781 cataaactct atgatttctc tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag
     6841 gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac atgttgttgt aaccgaacgg
     6901 gtcccatagc gccaaaacca aatctgccag cgtcaataga atgagcacga tgccgacaat
     6961 ggagctggct tggatagcga ttcgagttaa caacggccgg gatccactag ttctagagcg
     7021 gccgccaccg cgggacgtta cacaattcta atattaatta aattattgtt ttaagtatga
     7081 tagtaaatca cattacgccg cgttcgaatt aatagtggtc acttttttct tatctcttaa
     7141 gcaaaccgtt tgaataaatt actcatattt ttgttgttgt tggaaataga gcaaaacttt
     7201 ttttttcgtc gtgaagagaa taaaattctc tttgagacga aatgcattgg tatgtgttat
     7261 ctttagtagt attgataata tagtgtgtta aacattgcgc actgcaaaaa aaacatgctg
     7321 ttcgaattaa tagtggttgg ggctcgtaga aaacgaaaaa tatcttaagc tagcatagag
     7381 aatggagcaa aactcaattt gat
//
