pSE152: pFuGW-hUbCp-3CLpro-SV40NLS LOCUS pSE152 10151 bp DNA circular SYN 25-DEC-2024 DEFINITION pFuGW-hUbCp-3CLpro-SV40NLS ACCESSION pSE152 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10151) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software FEATURES Location/Qualifiers CDS 445..460 /gene="cPPT" /SECDrawAs="Gene" /SECStyleId=1 CDS 630..1834 /gene="hUbCp" /product="Ubiquitin-C promoter" /SECDrawAs="Gene" /SECStyleId=1 CDS 1869..1877 /gene="KOZAK" /SECDrawAs="Gene" /SECStyleId=1 CDS 1875..2820 /gene="3CLpro" /SECDrawAs="Gene" /SECStyleId=1 CDS 2796..2816 /gene="SV40NLS" /SECDrawAs="Gene" /SECStyleId=1 CDS 2846..3433 /gene="WPRE" /product="The WPRE seems to increase the expression of the marker gene when introduced in its 3' untranslated region. This element is believed to exert some posttranslational regulation. " /SECDrawAs="Gene" /SECStyleId=1 CDS 3620..3635 /gene="cPPT" /SECDrawAs="Gene" /SECStyleId=1 CDS 3620..3641 /gene="U3PPT" /product="reverse transcriptase-associated RNase H cleavage of the RNA at the polypurine tract (PPT), a short (ca. 11 to 19 nucleotides [nt]) string of purines located at the upstream border of the 3? long terminal repeat " /SECDrawAs="Gene" /SECStyleId=1 CDS 3957..4137 /gene="del3'LTR" /product="truncHIV-1_3_LTR" /SECDrawAs="Gene" /SECStyleId=1 CDS 4166..4393 /gene="bGH-Ter" /product="bGH_PA_terminator" /SECDrawAs="Gene" /SECStyleId=1 CDS complement(4882..5097) /gene="SV40_enhancer" /SECDrawAs="Gene" /SECStyleId=1 CDS 4894..5162 /gene="SV40p" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 5061..5138 /gene="SV40-Ori" /SECDrawAs="Region" /SECStyleId=1 CDS 5256..5323 /gene="EM7p" /SECDrawAs="Gene" /SECStyleId=1 CDS 5324..5695 /gene="Bleo" /SECDrawAs="Gene" /SECStyleId=1 CDS 5831..5950 /gene="SV40-pA" /SECDrawAs="Gene" /SECStyleId=1 CDS complement(7189..8019) /gene="Amp" /SECDrawAs="Gene" /SECStyleId=1 CDS 8391..8967 /gene="CMVp" /product="CMV_immearly_promoter" /SECDrawAs="Gene" /SECStyleId=1 CDS 8470..8757 /gene="CAG_enhancer" /SECDrawAs="Gene" /SECStyleId=1 CDS join(8987..10151,1..602) /gene="5' Viral freatures" /SECDrawAs="Gene" /SECStyleId=1 CDS 8987..9167 /gene="5'LTR" /product="HIV-1_5_LTR" /SECDrawAs="Gene" /SECStyleId=1 CDS 9278..9322 /gene="Psi" /SECDrawAs="Gene" /SECStyleId=1 CDS 9832..10065 /gene="RRE" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa 61 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg 121 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata 181 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac 241 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc 301 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca 361 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa 421 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 481 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 541 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt 601 aacccgtgtc ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc 661 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct 721 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag 781 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt 841 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct 901 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt 961 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg 1021 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga 1081 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct 1141 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag 1201 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa 1261 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc 1321 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt 1381 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg 1441 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg 1501 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg 1561 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt 1621 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg 1681 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt 1741 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc 1801 taaattctgg ccgtttttgg cttttttgtt agacgaagct tgggctgcag gtcgactcta 1861 gaggatccgc caccatgagt ggttttagaa aaatggcatt ccccagcggt aaagttgaag 1921 gatgtatggt ccaagtaacc tgtggtacca ctacccttaa tgggctttgg ttggacgacg 1981 tagtctactg cccccgacac gtaatctgca ctagtgagga tatgcttaat cccaattacg 2041 aagacctttt gattcggaaa tccaatcaca acttcctggt ccaagcgggc aacgtccaac 2101 tcagggttat tggacatagt atgcagaatt gcgtactgaa gctcaaagtc gatactgcaa 2161 accccaagac gcccaagtat aaattcgtcc gaatccaacc aggccaaaca ttttccgtat 2221 tggcttgcta taatggaagc cccagcggtg tctaccaatg tgcaatgaga ccaaacttta 2281 cgataaaggg ttcatttctg aacggctctt gcggttccgt tggttttaac atcgactatg 2341 actgtgtatc cttttgctac atgcaccata tggaactccc taccggtgtc cacgccggta 2401 cagatctgga aggaaatttc tacggtccgt tcgttgaccg gcaaaccgcg caagcggctg 2461 gaaccgacac aacgattaca gtgaatgtgc tcgcgtggct gtacgcagca gtcataaacg 2521 gagacaggtg gtttctgaac cgatttacga cgactctcaa tgacttcaac cttgttgcga 2581 tgaagtacaa ttacgagcca ctcacccagg accatgttga tatcctgggt cccctcagtg 2641 cccagacagg gatcgcagtt ctcgatatgt gcgcgtcact gaaggagctt ctccaaaatg 2701 gaatgaatgg gcggaccata cttggttccg cactcctcga agatgaattt actccatttg 2761 acgtggtcag acaatgctca ggtgttactt tccaaccaaa gaagaagcgg aaggtctagg 2821 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 2881 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2941 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 3001 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 3061 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 3121 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 3181 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 3241 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 3301 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3361 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3421 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa 3481 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg 3541 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 3601 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 3661 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc 3721 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc 3781 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt 3841 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt 3901 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt 3961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 4021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 4081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg 4141 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 4201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 4261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 4321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 4381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4981 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 5041 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 5101 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 5161 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 5221 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 5281 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 5341 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 5401 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 5461 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 5521 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 5581 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 5641 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 5701 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 5761 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 5821 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5941 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 6001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6061 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 6601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 8101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 8161 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca 8221 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 8281 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 8341 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 8401 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 8461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 8521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 8581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 8641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 8701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 8761 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 8821 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 8881 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 8941 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt 9001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 9061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 9121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 9181 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct 9241 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact 9301 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt 9361 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa 9421 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa 9481 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca 9541 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata 9601 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag 9661 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa 9721 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 9781 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 9841 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 9901 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 9961 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 10021 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 10081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 10141 tctggaacag a //