LOCUS       pCI_JUNO_GFPnes-        7100 bp DNA     circular SYN 18-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Nico
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        386..661
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /note="color: black; sequence: GGTTCGGCTTCTGGCGTGTGACC"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /note="color: black; sequence: GCAACGTGCTGGTTATTGTG"
     primer_bind     1784..1810
                     /label=Juno F NheI
                     /note="color: black; sequence: 
                     TAAgctagcCTCTTTGGCATCAGGAGGAGC"
     primer_bind     1790..1810
                     /label=JunoF-EcoRV
                     /note="color: black; sequence: 
                     aacgGATATCTCTTTGGCATCAGGAGGAGC"
     primer_bind     1790..1810
                     /label=JunoF-XhoI
                     /note="color: black; sequence: 
                     TAATctcgagCTCTTTGGCATCAGGAGGAGC"
     primer_bind     1835..1935
                     /label=mJUNO F EcoRI
                     /note="color: black; sequence: 
                     AATAGAATTCATGGCACAGTGGTGGCAGATCCTCTTGGGGTTGTGGGCAGTCCTACCCA
                     CCTTGGCAGGGGACAAACTGCTCAGCGTCTGCATGAACTCCAAGCGCCAC"
     CDS             1837..2571
                     /codon_start=1
                     /label=Juno
                     /note="color: #cc99ff"
                     /translation="MAQWWQILLGLWAVLPTLAGDKLLSVCMNSKRHKQEPGPEDELYQ
                     ECRPWEDNACCTRSTSWEAHLEEPLLFNFSMMHCGLLTPACRKHFIQAICFHECSPNLG
                     PWIQPVVPNGQEEQRVWGVPLCQEDCEDWWRACHSSLTCKSNWLHGWDWSEEKKHCPAH
                     EPCLPFSYHFPTPDDLCEKIWNNTFKASPERRNSGRCLQKWFEPTLSNPNVEVALHFAG
                     SALAPQLSYTLPAFSLCLLFHP"
     primer_bind     complement(2636..2662)
                     /label=Juno R SmaI
                     /note="color: black; sequence: 
                     ATACcccgggTGCCCCCAACATGAATAGCC"
     primer_bind     complement(2636..2655)
                     /label=Juno R-NotI
                     /note="color: black; sequence: 
                     attGCGGCCGCTGCCCCCAACATGAATAGCC"
     primer_bind     complement(2636..2655)
                     /label=JunoR-NheI
                     /note="color: black; sequence: 
                     ATgctagcTGCCCCCAACATGAATAGCC"
     misc_feature    join(2669..3243,3244..3246,3247..3255)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 2669 .. 3243 / #a6acb3
                        2: 3244 .. 3246 / #a6acb3 / ATG
                        3: 3247 .. 3255 / #a6acb3"
     primer_bind     complement(2836..2853)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="color: black; sequence: GCATTCCTTTGGCGAGAG"
     primer_bind     3063..3082
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     CDS             join(3261,3262..3970)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 3261 .. 3261 / #05fd14 / 1a
                        2: 3262 .. 3970 / #05fd14"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELY"
     primer_bind     complement(3301..3322)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(3562..3581)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     3909..3930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     misc_feature    3982..4014
                     /label=NES
                     /note="color: #800080; direction: RIGHT"
     primer_bind     complement(4092..4111)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
                     /note="color: black; sequence: TTTTGGCAGAGGGAAAAAGA"
     polyA_site      4157..4212
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4211..4230)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
                     /note="color: black; sequence: CCCATATGTCCTTCCGAGTG"
     primer_bind     complement(4603..4619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4603..4619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(4616..4638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    4627..4643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(4651..4657,4658..4675,4676..4681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 4651 .. 4657 / #ffffff / -10
                        2: 4658 .. 4675 / #ffffff
                        3: 4676 .. 4681 / #ffffff / -35"
     protein_bind    4696..4717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        4776..4971
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4822..4957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4884..4903
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(4907..4918)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     polyA_signal    4977..5111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5014..5033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     5068..5087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(5179..5196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(5350..5938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5430..5449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(join(6109..6900,6901..6969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6109 .. 6900 / #ccffcc
                        2: 6901 .. 6969 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6732..6751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(6970..7074)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct cgagagttcg
     1741 aagccttaag tacgcgtagg cgcgccttaa ttaacgttta aacgctagcc tctttggcat
     1801 caggaggagc aacaggaggg ccctgccttg aaggtcatgg cacagtggtg gcagatcctc
     1861 ttggggttgt gggcagtcct acccaccttg gcaggggaca aactgctcag cgtctgcatg
     1921 aattccaagc gccacaagca agaacctggc ccagaagacg aactctacca ggagtgcagg
     1981 ccttgggagg acaatgcctg ctgcacacgt tccacaagtt gggaagccca ccttgaggag
     2041 cccttgctct ttaacttcag catgatgcac tgtggactgc tgaccccggc ctgtcgcaag
     2101 cacttcatcc aggccatctg cttccatgag tgttccccca acctggggcc ttggatccag
     2161 ccggtggtcc caaacgggca ggaagagcag cgggtttggg gcgtgccgct gtgccaggag
     2221 gattgtgagg actggtggcg agcctgccac tcatctttga cttgtaaatc caactggctc
     2281 catggctggg actggagtga ggagaagaaa cactgccccg cacatgagcc ctgcctccct
     2341 ttttcgtatc acttccccac cccggatgac ctgtgtgaga agatctggaa caacacattc
     2401 aaggccagtc ccgagcgcag gaacagtggg cggtgtctgc agaagtggtt tgagcccacc
     2461 cttagcaacc ccaacgtgga agtggccctc cactttgccg gctctgcctt agccccacag
     2521 ctctcttaca ccctcccagc cttctctctg tgcctgttgt tccatccctg atccctgctt
     2581 ccctctccaa cccacatttc ctgatccttc tgggccatgg ccatctccat cctcaggcta
     2641 ttcatgttgg gggcacccgg gggatccgcc cctctccctc ccccccccct aacgttactg
     2701 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     2761 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     2821 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     2881 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     2941 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3001 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3061 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3121 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     3181 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3241 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3301 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3361 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3421 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     3481 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3541 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3601 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3661 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3721 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3781 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3841 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3901 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3961 gagctgtaca tgagcggcgg cctgcagaag aagctggagg agctggagct ggactaatgt
     4021 acaagtaaag cggccggccg ccagcacagt ggtcgatcga ccaatgccct ggctcacaaa
     4081 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     4141 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     4201 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     4261 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     4321 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     4381 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     4441 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     4501 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca ccgtcgacca
     4561 gctggtcgac ggtgcaccgt cgaccagctt ggcgtaatca tggtcatagc tgtttcctgt
     4621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4741 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     4801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     4981 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5041 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5101 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5281 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5341 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5521 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     5581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5761 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7081 tccccgaaaa gtgccacctg
//
