LOCUS       Exported                8362 bp ds-DNA     circular SYN 28-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1784..1814
                     /label=AFF-1 Fwd
     CDS             1790..3556
                     /codon_start=1
                     /label=AFF-1
                     /translation="MVLWQWSIAVAICLVMVTEARLRRHHRKRRFVSSNFDEFYCGESA
                     HAQSQFEEERESNSSKVSSVHSTQFNWGLDNTICIKLQNVVHVLKYERLEQRYPIENSY
                     TFSVPLIDTNCKCHCYGFGTNDVCNVEKYADDRNCTTSSEFPTCYTKYHPAVEPLDCPV
                     TSIPAKACCDIKLKPRDGRMFRAVKLQQPINDMIISHSIFANNSGKMMKVLGPDEFRIN
                     LLKGKEQFELTEYHRISVQLVASSPQQQLREGMYYFPEENHNDLREGKINEITESDLDK
                     LGWYRRVGNDWQVATSGLLLRNAHKVVIKNCKGQVHMDQFSGTKNFVLRGTQYNDTYNE
                     RRVSDNNFVRSVKVDESSREITIVHEHGTAAQVSLKTDTRPNLTKSQSLLANFTGSITL
                     DHDGNRMLNVTFFGVKGTVHIKMYVNDRKLIATFACTAQFGTSLKDDGSRISLPSTINQ
                     AQWVCILPDEQPTKSEICKWIPYEEKAMRTPRQEQSWSKGHSPCSQAECNSLKSGVSDL
                     FPWIMNFDYFMAHGGDFTEWLKIGIHIVIAVGLLLLLILLFTKCLVPLACCSLSIPFKN
                     RNKKKKKKNSSDY"
     primer_bind     complement(3557..3592)
                     /label=AFF-1 Rvs
     CDS             3557..3580
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3597..4183
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3764..3781)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3991..4010
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4190..4567
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4580..4589
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4586..5263
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4716..4734)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4984..5003
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5178..5201
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5354..5373)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      5419..5474
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(5473..5492)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5865..5881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5865..5881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5878..5900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5889..5905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5913..5943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5958..5979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6038..6233
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6084..6219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6146..6165
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6169..6180)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    6239..6373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6276..6295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6330..6349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6441..6458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6612..7200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6692..6711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7371..8231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7994..8013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8232..8336)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct cgagagttcg
     1741 aagccttaag tacgcgtagg cgcgccttaa ttaacgttta aacgctagca tggtactgtg
     1801 gcaatggtca atagccgttg ccatatgtct tgtgatggtt acagaagctc gtctcagaag
     1861 acatcacagg aaacgacgat ttgtatctag taattttgat gaattttatt gtggagaaag
     1921 tgcacatgct cagtcacagt ttgaagagga gcgagaatca aattcctcaa aagtctcatc
     1981 agtacacagt actcaattca attggggact tgataataca atttgtataa aactccagaa
     2041 tgtggttcat gttttaaaat acgaacgcct cgaacaaaga tatcctattg aaaactccta
     2101 cacattttcg gttccattaa ttgacacaaa ctgtaaatgt cattgttatg gttttgggac
     2161 aaatgacgtt tgcaatgtgg aaaagtacgc tgacgacaga aattgtacga cgagctcaga
     2221 gtttcctaca tgttacacca aatatcaccc agcagttgag ccactagatt gtccagttac
     2281 aagtattcct gccaaggcat gctgcgacat caagttaaaa ccacgcgatg gcagaatgtt
     2341 ccgagctgtg aaacttcagc aaccaatcaa tgacatgata atttctcatt ctatttttgc
     2401 aaacaacagt ggaaaaatga tgaaagttct gggaccagat gaatttagga taaatcttct
     2461 gaaagggaag gaacaatttg agttaactga ataccacaga atatccgttc aattagttgc
     2521 atcttctcct caacaacaac ttcgcgaagg gatgtattat tttccagagg aaaaccacaa
     2581 cgatctgcgt gagggtaaaa ttaatgaaat aactgaaagt gatttggata aacttggatg
     2641 gtatagaaga gttggaaatg attggcaagt tgctacaagt ggattactac ttagaaatgc
     2701 acataaagtg gtgataaaaa attgcaaagg acaagttcat atggatcaat tttccggaac
     2761 caaaaacttt gttttacgtg gaactcagta taacgatact tacaatgaac gaagagtatc
     2821 tgacaataat tttgtaagaa gtgtgaaagt tgacgagtct tctcgagaaa ttacaattgt
     2881 tcatgaacat ggaaccgctg cacaagtttc tctgaaaact gacactcgtc caaatctaac
     2941 aaaaagtcaa tcacttttgg caaacttcac tggaagtatc acgttagatc atgatggaaa
     3001 tcgaatgctt aatgtcactt tctttggcgt caaaggaact gttcatatca aaatgtatgt
     3061 caacgatcga aagctcatcg cgacttttgc atgtactgct caatttggaa cgtctctgaa
     3121 agatgatggt agtagaataa gtcttccatc gactataaat caagctcaat gggtgtgtat
     3181 tcttcccgat gagcagccaa caaaatcaga aatatgcaaa tggattccat atgaggaaaa
     3241 agcaatgaga actccgagac aagaacaaag ttggtcaaaa ggacattcac cgtgctcaca
     3301 agcagaatgt aatagtctga aaagtggagt gagcgacttg ttcccatgga ttatgaattt
     3361 tgattatttt atggctcatg gtggggattt cacagagtgg ctaaaaattg gaatccatat
     3421 agtgatagct gttggtctcc tgttattatt aattcttctc ttcacaaaat gccttgttcc
     3481 gttggcatgt tgctccctgt caataccatt taaaaatagg aacaaaaaga agaaaaagaa
     3541 gaattcatct gattacgact acaaggacga cgatgacaaa taacccgggg gatccgcccc
     3601 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3661 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3721 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3781 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3841 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     3901 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     3961 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4021 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca
     4081 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4141 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtta tgccagagcc
     4201 agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa
     4261 gaaaggcggc aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa
     4321 ggttctgaag caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa
     4381 ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta
     4441 caacaagcgc tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc
     4501 tggggagttg gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag
     4561 cgctaaggat ccaccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat
     4621 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4681 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     4741 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
     4801 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4861 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4921 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4981 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
     5041 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     5101 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
     5161 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5221 acagtacgag cgcgccgagg gccgccactc caccggcgcc taagcggccg caattcactc
     5281 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     5341 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     5401 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     5461 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     5521 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     5581 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     5641 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5701 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5761 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg caccgtcgac
     5821 cagctggtcg acggtgcacc gtcgaccagc ttggcgtaat catggtcata gctgtttcct
     5881 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5941 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6001 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     6061 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6121 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6181 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     6241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6361 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6421 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6781 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     6841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7021 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7141 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7321 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7681 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7981 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8341 tttccccgaa aagtgccacc tg
//
