LOCUS       p5E\itga11-cFos         6178 bp    DNA     circular     29-MAY-2025
SOURCE      
  ORGANISM  
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
COMMENT     228.p5E-MCS, Tol2kit v1.0
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15991886|
COMMENT     VNTDBDATE|-15991886|
COMMENT     LSOWNER|
COMMENT     VNTNAME|p5E itga11-cFos|
COMMENT     VNTAUTHORNAME|Enyedi Balázs|
COMMENT     VNTAUTHOREML|enyedib@eok.sote.hu|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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                     /label=att4_shared
     rep_origin      5502..6175
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             4572..5381
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
                     /vntifkey="4"
                     /label=kanR
     primer_bind     complement(4439..4459)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     primer_bind     537..552
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
     promoter        complement(4204..4223)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="30"
                     /label=T3
     promoter        complement(4416..4435)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="30"
                     /label=T7
     primer_bind     689..711
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=T7\primer
     terminator      268..295
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
                     /vntifkey="43"
                     /label=T2
     terminator      427..470
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
                     /vntifkey="43"
                     /label=T1
     misc_feature    723..3922
                     /vntifkey="21"
                     /label=itga11_3.2kb-prom
     misc_feature    3960..4175
                     /vntifkey="21"
                     /label=cFos_minimal\promoter
     misc_feature    723..4175
                     /vntifkey="21"
                     /label=itga11-cFos
BASE COUNT     1709 a      1263 c      1400 g      1806 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta 
      721 ccgaattgct ctgctttgat ggaaatattt gatgaaagga aagacagatg actttttaag 
      781 tactcttaat gaatgcagcg ctctttactt tggtcatttt tgttttaagc aaaatcagct 
      841 cagtgttgat cagatgtttg gtctctttgg tttcaaactt aaggcacata catagtagaa 
      901 actgcaacat cctcatacta ttaagaattt atatattctt tattaatcgt gtgttcaata 
      961 gtatgtaaca ttgatttttc aactcagatc ttcctccaca atttggtgga ttagttttct 
     1021 ggcagtcaga aggtcgtgag ttctttaatg tgcttcttta atgttttatt gtttatctat 
     1081 aatattgggt atcatggatt ttgagtatat aatggtacaa ataatgagat aaaaatgtat 
     1141 ttttattgtt ttggacagtc ggaattccag acatgaataa gtgaatgatg ataattgaat 
     1201 attattttcc tatacaatgg agataatgct tttaaaggta aaggaaatat ttaaatatct 
     1261 gtggttaatt acagtatagt aaaaacgagt ttttaattat taagtggtcg tctaattaat 
     1321 gacataatgt gattgacttt tgcacattgg aggaaggatg acactatggc tgaagtattt 
     1381 cctgacaggt aatgttctgt tttaaaatta tttagtcacg caaagctgat gtggattgtg 
     1441 ctgttttacg agtgagttat atgcacagaa gtgttgttca ccaacttaaa tcctccggca 
     1501 aaacgttgat gtgactattt tcagtttcat aagggacctt ttacagcatc gacagattat 
     1561 tatttagttt aacaacaaag catcagttaa tagagctatt cctccgagcc tgctttggtt 
     1621 catttttgaa caatgacacc ctataatttt tatcatgcta ctttgaatat ttgatatgaa 
     1681 atagcacgca agtatggctt agtaataagt gtaatttttg cacgccgcca gccaaccact 
     1741 aactacattt ctagctggcg aatgacatta actaataggt tgtctgctgt catctttaag 
     1801 gtgcgcacgt ttgtgtgcat gcaaaagcat ggctttggac agcaggggag ggactttgtt 
     1861 ttaaagatat catgctagcc ggttagtatt ttggcatatc acctactaca cccttaatga 
     1921 gtgctaatga tgaccttttg aatgataata ttaactatgt agattaacgc accaaaaatg 
     1981 taaaaccgcg agccctgaac gtgatagttt tctgaaataa aatccatgtt tacaccaaag 
     2041 atggttgcta taactatagt gatgacgata ttaacaatgc acaataatta aataatgtgc 
     2101 tttaactact ataaaattca ttaatgaaat tgacctgggc gtcacggtag cgcaatgttt 
     2161 agcacaatcg cctcacagta agaaggtcac tggttcgagc ctgggctggg tcagttggca 
     2221 tttctgtctg gagattgcat gttctccccg tatttgcatg ctccagttta ccccacaagt 
     2281 ccaaagacat gtggtatagg tgaattgaaa aggctaaatt gtccctagtg catgtgtgtg 
     2341 aatgagtgtg catggatgtt taccattgat gggttgcaga tgaaaggaca taatctgcgt 
     2401 aaaacatatg ctggataagt tggtggttca ttccactgtg tacagccttt aaagggatag 
     2461 ttcacctaaa attgaaatat ctgtcatcgt ttactcaccc ttcacatata acaaaccagt 
     2521 ttgagtttct tttttctgtt gaacacaaaa aagaagtgat tttgataaat ggaaacctgc 
     2581 aaccattgac tcctatcgta tttgtttttc atactatggg tggttacaca tggttgcagg 
     2641 tttctttaat ttatcatgta taatatatgt cttgtggcat ggtggctcag tggttagcac 
     2701 tgttgcctca cagcaagaag gtcactgggt tgagtcccag ctgggccaga tagcatttct 
     2761 gtgtggtgtt tgcatgttta ccctgtgttg gtgcgtgttt cccccggtgc tccggtttcc 
     2821 cccacaattt aaagacatgt gatatagatg aattgaataa actaaattgg ccatatgagt 
     2881 gtgtgaatga gagtgtatgg ctgtttcgca gtactgggtt gcagctagaa gggcattcgc 
     2941 tgcgtaaaac acatgctgga atagttgctg gttcattcct ctgtcacaac ttataaaata 
     3001 gagactaagc ttaaggaaaa tgaatgaatg aatatactgt atgtattttt aaatatatct 
     3061 tctttgttca acacaaaaaa atgagctcca taaagatttg gaaacacttg agggtgagta 
     3121 aataaacatt ttgtggtgaa atttgctttt aagtctgaca gttgtcttgt gaagtatata 
     3181 aacaggatat tatgcatgtg tttggtagtt taattgtaag attacattta cactgcatgt 
     3241 tcattttgac caaaaactaa gcaacagcgg ttttgatatt ttcaaggtca agttgaaatg 
     3301 gaggtgggcc atgtttcact attagagcag ccagaacgtc agtattagtc attatataat 
     3361 ctgtaattat atgcttgggt agcgtggttt ggatggtgtc tgaggttctg ctgtgagttt 
     3421 gtcagtgggc tttatatggt aacttgcagc tacagtatgt taaccacaaa tctcggcatc 
     3481 agtcaacttg gagggcatct tctccgaaag tatgcagcct aaaatgcatg ttaagcgaat 
     3541 tacaaaatta cggaagatca ggttttgcca tagaggtgat atacagtgat ccctaaaacc 
     3601 tgaatttcca tattcaaagc atttagcaat gagctcataa gaaagtgacc caaactccaa 
     3661 cgtagatgga cagacacaca gagggaggaa gtaaaagaga gagaaagaaa aagagaaaag 
     3721 atctgtctgc tggctgaatg agcacctcct tttggttcgg ccacagtccc ctcctccctg 
     3781 cttcagatat agagtgtttt taggagcagt agagtcaatc agagccgggt gtacaggaga 
     3841 gcgcactgtc aggatatttc atccttctgc acacacacac atacaccaac acacacattt 
     3901 tcttacgttc tccctctcca acggatccac tagttctaga gcggccgcca ccgcggtggc 
     3961 cagtgacgta ggaagtccat ccattcacag cgcttctata aaggcgccag ctgaggcgcc 
     4021 tactactcca accgcgactg cagcgagcaa ctgagaagac tggatagagc cggcggttcc 
     4081 gcgaacgagc agtgaccgcg ctcccaccca gctctgctct gcagctccca ccagtgtcta 
     4141 cccctggacc ccttgccggg ctttccccaa acttcccacc gcggtggagc tccagctttt 
     4201 gttcccttta gtgagggtta attcaagttt gtacaaaaaa gttgaacgag aaacgtaaaa 
     4261 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 
     4321 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta 
     4381 gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca 
     4441 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg 
     4501 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata 
     4561 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca 
     4621 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga 
     4681 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag 
     4741 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta 
     4801 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca 
     4861 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa 
     4921 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt 
     4981 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg 
     5041 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct 
     5101 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt 
     5161 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac 
     5221 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt 
     5281 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga 
     5341 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt 
     5401 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat 
     5461 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     5641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     5881 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc 
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt 
//
