LOCUS       pCMV-MMLVgag-D3A       13704 bp DNA     circular SYN 29-MAY-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13704
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     CDS             complement(170..1030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  170 ..  961 / #ccffcc
                        2:  962 .. 1030 / #ccffcc / signal sequence
                     Cleavage site after base 961"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(1161..1616)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     1758..1774
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        1784..1802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     enhancer        1923..2302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2303..2506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          2618..3190
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
                     /note="color: #------"
     CDS             3288..3704
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3288..3677
                     /label=Matrix protein_p15
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3678..3929
                     /label=RNA binding protein_p12
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3930..4718
                     /label=Capsid protein_p30
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4719..4886
                     /label=Nucleocapsid protein_p10
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4917..4943
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     CDS             4956..4982
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     CDS             4995..5021
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     misc_feature    5022..5054
                     /label=Cleavable linker v2.4
                     /note="color: #ffff00"
     CDS             5055..5078
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             5079..5132
                     /codon_start=1
                     /label=bp SV40 NLS
                     /note="color: #993366"
                     /translation="KRTADGSEFESPKKKRKV"
     misc_feature    5133..6035
                     /label=DNMT3A
                     /note="color: #ffcc99; direction: RIGHT"
     misc_feature    6036..6116
                     /label=Linker
                     /note="color: #000000"
     misc_feature    6117..6761
                     /label=DNMT3L
                     /note="color: #99cc00; direction: RIGHT"
     misc_feature    6762..7001
                     /label=XTEN80
                     /note="color: #ccffff"
     CDS             7002..11105
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /note="This feature has 5 segments:
                        1:  7002 ..  7028 / #993366
                        2:  7029 ..  7031 / #cc99b2
                        3:  7032 ..  9518 / #993366
                        4:  9519 ..  9521 / #cc99b2
                        5:  9522 .. 11105 / #993366"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11109..11135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             11115..11141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /note="color: #cc99b2"
                     /translation="YDVPDYASL"
     CDS             11154..11174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             11181..11201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             11241..11936
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             11952..12167
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /note="color: #993366"
                     /translation="MDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLE
                     NYKNLVSLGYQLTKPDVILRLEKGEEP"
     CDS             12210..12230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    12301..12356
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     primer_bind     complement(12714..12730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    12738..12754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(12762..12792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 12762 .. 12768 / #ffffff / -10
                        2: 12769 .. 12786 / #ffffff
                        3: 12787 .. 12792 / #ffffff / -35"
     protein_bind    12807..12828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(13116..13704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1741 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     1801 gggcgaattg gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac
     1861 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
     1921 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1981 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2041 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2101 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2161 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2221 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2281 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2341 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2401 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2461 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2521 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2581 atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
     2641 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2701 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2761 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     2821 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     2881 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     2941 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     3001 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     3061 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     3121 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     3181 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
     3241 attcctcgag ggccggatcc gaattcctgt atttgtctga aaacatgggc cagactgtta
     3301 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     3361 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     3421 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     3481 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     3541 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     3601 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     3661 ctcgatcctc cctttatcca gccctcactc cttctctagg cgccaaacct aaacctcaag
     3721 ttctttctga cagtgggggg ccgctcatcg acctacttac agaagacccc ccgccttata
     3781 gggacccaag accaccccct tccgacaggg acggaaatgg tggagaagcg acccctgcgg
     3841 gagaggcacc ggacccctcc ccaatggcat ctcgcctacg tgggagacgg gagccccctg
     3901 tggccgactc cactacctcg caggcattcc ccctccgcgc aggaggaaac ggacagcttc
     3961 aatactggcc gttctcctct tctgaccttt acaactggaa aaataataac ccttcttttt
     4021 ctgaagatcc aggtaaactg acagctctga tcgagtctgt cctcatcacc catcagccca
     4081 cctgggacga ctgtcagcag ctgttgggga ctctgctgac cggagaagaa aaacaacggg
     4141 tgctcttaga ggctagaaag gcggtgcggg gcgatgatgg gcgccccact caactgccca
     4201 atgaagtcga tgccgctttt cccctcgagc gcccagactg ggattacacc acccaggcag
     4261 gtaggaacca cctagtccac tatcgccagt tgctcctagc gggtctccaa aacgcgggca
     4321 gaagccccac caatttggcc aaggtaaaag gaataacaca agggcccaat gagtctccct
     4381 cggccttcct agagagactt aaggaagcct atcgcaggta cactccttat gaccctgagg
     4441 acccagggca agaaactaat gtgtctatgt ctttcatttg gcagtctgcc ccagacattg
     4501 ggagaaagtt agagaggtta gaagatttaa aaaacaagac gcttggagat ttggttagag
     4561 aggcagaaaa gatctttaat aaacgagaaa ccccggaaga aagagaggaa cgtatcagga
     4621 gagaaacaga ggaaaaagaa gaacgccgta ggacagagga tgagcagaaa gagaaagaaa
     4681 gagatcgtag gagacataga gagatgagca agctattggc cactgtcgtt agtggacaga
     4741 aacaggatag acagggagga gaacgaagga ggtcccaact cgatcgcgac cagtgtgcct
     4801 actgcaaaga aaaggggcac tgggctaaag attgtcccaa gaaaccacga ggacctcggg
     4861 gaccaagacc ccagacctcc ctcctgaccc tagatgactc tggcggctca cttcaactgc
     4921 ctccacttga aagactgaca ctgggatcat tacaattacc tcctttagaa cgattaacac
     4981 tcggttcact acagcttccg cctcttgaga gattgacatt aacgtccacg ctgctaatgg
     5041 agaactcgtc tggagattac aaagacgatg acgataagaa acggacagcc gacggaagcg
     5101 agttcgagtc accaaagaag aagcggaaag tcaaccatga ccaggaattt gaccccccaa
     5161 aggtttaccc acctgtgcca gctgagaaga ggaagcccat ccgcgtgctg tctctctttg
     5221 atgggattgc tacagggctc ctggtgctga aggacctggg catccaagtg gaccgctaca
     5281 ttgcctccga ggtgtgtgag gactccatca cggtgggcat ggtgcggcac cagggaaaga
     5341 tcatgtacgt cggggacgtc cgcagcgtca cacagaagca tatccaggag tggggcccat
     5401 tcgacctggt gattggaggc agtccctgca atgacctctc cattgtcaac cctgcccgca
     5461 agggacttta tgagggtact ggccgcctct tctttgagtt ctaccgcctc ctgcatgatg
     5521 cgcggcccaa ggagggagat gatcgcccct tcttctggct ctttgagaat gtggtggcca
     5581 tgggcgttag tgacaagagg gacatctcgc gatttcttga gtctaacccc gtgatgattg
     5641 acgccaaaga agtgtctgct gcacacaggg cccgttactt ctggggtaac cttcctggca
     5701 tgaacaggcc tttggcatcc actgtgaatg ataagctgga gctgcaagag tgtctggagc
     5761 acggcagaat agccaagttc agcaaagtga ggaccattac caccaggtca aactctataa
     5821 agcagggcaa agaccagcat ttccccgtct tcatgaacga gaaggaggac atcctgtggt
     5881 gcactgaaat ggaaagggtg tttggcttcc ccgtccacta cacagacgtc tccaacatga
     5941 gccgcttggc gaggcagaga ctgctgggcc gatcgtggag cgtgccggtc atccgccacc
     6001 tcttcgctcc gctgaaggaa tattttgctt gtgtgtctag cggcaatagt aacgctaaca
     6061 gccgcgggcc gagcttcagc agcggcctgg tgccgttaag cttgcgcggc agccatatgg
     6121 gccctatgga gatatacaag acagtgtctg catggaagag acagccagtg cgggtactga
     6181 gcctcttcag aaacatcgac aaggtactaa agagtttggg cttcttggaa agcggttctg
     6241 gttctggggg aggaacgctg aagtacgtgg aagatgtcac aaatgtcgtg aggagagacg
     6301 tggagaaatg gggccccttt gacctggtgt acggctcgac gcagccccta ggcagctctt
     6361 gtgatcgctg tcccggctgg tacatgttcc agttccaccg gatcctgcag tatgcgctgc
     6421 ctcgccagga gagtcagcgg cccttcttct ggatattcat ggacaatctg ctgctgactg
     6481 aggatgacca agagacaact acccgcttcc ttcagacaga ggctgtgacc ctccaggatg
     6541 tccgtggcag agactaccag aatgctatgc gggtgtggag caacattcca gggctgaaga
     6601 gcaagcatgc gcccctgacc ccaaaggaag aagagtatct gcaagcccaa gtcagaagca
     6661 ggagcaagct ggacgccccg aaagttgacc tcctggtgaa gaactgcctt ctcccgctga
     6721 gagagtactt caagtatttt tctcaaaact cacttcctct tggagggccg agctctggcg
     6781 cacccccacc aagtggaggg tctcctgccg ggtccccaac atctactgaa gaaggcacca
     6841 gcgaatccgc aacgcccgag tcaggccctg gtacctccac agaaccatct gaaggtagtg
     6901 cgcctggttc cccagctgga agccctactt ccaccgaaga aggcacgtca accgaaccaa
     6961 gtgaaggatc tgcccctggg accagcactg aaccatctga gatggacaag aagtattcta
     7021 tcggactggc catcgggact aatagcgtcg ggtgggccgt gatcactgac gagtacaagg
     7081 tgccctctaa gaagttcaag gtgctcggga acaccgaccg gcattccatc aagaaaaatc
     7141 tgatcggagc tctcctcttt gattcagggg agaccgctga agcaacccgc ctcaagcgga
     7201 ctgctagacg gcggtacacc aggaggaaga accggatttg ttaccttcaa gagatattct
     7261 ccaacgaaat ggcaaaggtc gacgacagct tcttccatag gctggaagaa tcattcctcg
     7321 tggaagagga taagaagcat gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg
     7381 cctatcacga gaaataccca accatctacc atcttcgcaa aaagctggtg gactcaaccg
     7441 acaaggcaga cctccggctt atctacctgg ccctggccca catgatcaag ttcagaggcc
     7501 acttcctgat cgagggcgac ctcaatcctg acaatagcga tgtggataaa ctgttcatcc
     7561 agctggtgca gacttacaac cagctctttg aagagaatcc catcaatgca agcggagtcg
     7621 atgccaaggc cattctgtca gcccggctgt caaagagccg cagacttgag aatcttatcg
     7681 ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa cctgattgct ctttcacttg
     7741 ggctgactcc caatttcaag tctaatttcg acctggcaga ggatgccaag ctgcaactgt
     7801 ccaaggacac ctatgatgac gatctcgaca acctcctggc ccagatcggt gaccaatacg
     7861 ccgacctttt ccttgctgct aagaatcttt ctgacgccat cctgctgtct gacattctcc
     7921 gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc aatgattaag cggtatgatg
     7981 agcaccacca ggacctgacc ctgcttaagg cactcgtccg gcagcagctt ccggagaagt
     8041 acaaggaaat cttctttgac cagtcaaaga atggatacgc cggctacatc gacggaggtg
     8101 cctcccaaga ggaattttat aagtttatca aacctatcct tgagaagatg gacggcaccg
     8161 aagagctcct cgtgaaactg aatcgggagg atctgctgcg gaagcagcgc actttcgaca
     8221 atgggagcat tccccaccag atccatcttg gggagcttca cgccatcctt cggcgccaag
     8281 aggacttcta cccctttctt aaggacaaca gggagaagat tgagaaaatt ctcactttcc
     8341 gcatccccta ctacgtggga cccctcgcca gaggaaatag ccggtttgct tggatgacca
     8401 gaaagtcaga agaaactatc actccctgga acttcgaaga ggtggtggac aagggagcca
     8461 gcgctcagtc attcatcgaa cggatgacta acttcgataa gaacctcccc aatgagaagg
     8521 tcctgccgaa acattccctg ctctacgagt actttaccgt gtacaacgag ctgaccaagg
     8581 tgaaatatgt caccgaaggg atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg
     8641 caattgtgga ccttctgttc aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg
     8701 actatttcaa gaaaattgaa tgcttcgact ctgtggagat tagcggggtc gaagatcggt
     8761 tcaacgcaag cctgggtacc taccatgatc tgcttaagat catcaaggac aaggattttc
     8821 tggacaatga ggagaacgag gacatccttg aggacattgt cctgactctc actctgttcg
     8881 aggaccggga aatgatcgag gagaggctta agacctacgc ccatctgttc gacgataaag
     8941 tgatgaagca acttaaacgg agaagatata ccggatgggg acgccttagc cgcaaactca
     9001 tcaacggaat ccgggacaaa cagagcggaa agaccattct tgatttcctt aagagcgacg
     9061 gattcgctaa tcgcaacttc atgcaactta tccatgatga ttccctgacc tttaaggagg
     9121 acatccagaa ggcccaagtg tctggacaag gtgactcact gcacgagcat atcgcaaatc
     9181 tggctggttc acccgctatt aagaagggta ttctccagac cgtgaaagtc gtggacgagc
     9241 tggtcaaggt gatgggtcgc cataaaccag agaacattgt catcgagatg gccagggaaa
     9301 accagactac ccagaaggga cagaagaaca gcagggagcg gatgaaaaga attgaggaag
     9361 ggattaagga gctcgggtca cagatcctta aagagcaccc ggtggaaaac acccagcttc
     9421 agaatgagaa gctctatctg tactaccttc aaaatggacg cgatatgtat gtggaccaag
     9481 agcttgatat caacaggctc tcagactacg acgtggacgc catcgtccct cagagcttcc
     9541 tcaaagacga ctcaattgac aataaggtgc tgactcgctc agacaagaac cggggaaagt
     9601 cagataacgt gccctcagag gaagtcgtga aaaagatgaa gaactattgg cgccagcttc
     9661 tgaacgcaaa gctgatcact cagcggaagt tcgacaatct cactaaggct gagaggggcg
     9721 gactgagcga actggacaaa gcaggattca ttaaacggca acttgtggag actcggcaga
     9781 ttactaaaca tgtcgcccaa atccttgact cacgcatgaa taccaagtac gacgaaaacg
     9841 acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc caagctggtc agcgatttca
     9901 gaaaggactt tcaattctac aaagtgcggg agatcaataa ctatcatcat gctcatgacg
     9961 catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg
    10021 agttcgtgta cggagactac aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc
    10081 aggagatcgg aaaggccacc gcaaagtact tcttctacag caacatcatg aatttcttca
    10141 agaccgaaat cacccttgca aacggtgaga tccggaagag gccgctcatc gagactaatg
    10201 gggagactgg cgaaatcgtg tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc
    10261 tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta
    10321 aggaatcaat cctccccaag cgcaactccg acaagctcat tgcaaggaag aaggattggg
    10381 accctaagaa gtacggcgga ttcgattcac caactgtggc ttattctgtc ctggtcgtgg
    10441 ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt gaaggaactg ctgggtatca
    10501 ccattatgga gcgcagctcc ttcgagaaga acccaattga ctttctcgaa gccaaaggtt
    10561 acaaggaagt caagaaggac cttatcatca agctcccaaa gtatagcctg ttcgaactgg
    10621 agaatgggcg gaagcggatg ctcgcctccg ctggcgaact tcagaagggt aatgagctgg
    10681 ctctcccctc caagtacgtg aatttcctct accttgcaag ccattacgag aagctgaagg
    10741 ggagccccga ggacaacgag caaaagcaac tgtttgtgga gcagcataag cattatctgg
    10801 acgagatcat tgagcagatt tccgagtttt ctaaacgcgt cattctcgct gatgccaacc
    10861 tcgataaagt ccttagcgca tacaataagc acagagacaa accaattcgg gagcaggctg
    10921 agaatatcat ccacctgttc accctcacca atcttggtgc ccctgccgca ttcaagtact
    10981 tcgacaccac catcgaccgg aaacgctata cctccaccaa agaagtgctg gacgccaccc
    11041 tcatccacca gagcatcacc ggactttacg aaactcggat tgacctctca cagctcggag
    11101 gggatgccta tccctatgac gtgcccgatt atgccagcct gggcagcggc tcccccaaga
    11161 aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacggcatt ggtagtggga
    11221 gcaacggcag cagcggatcc agcgagctga ttaaggagaa catgcacatg aagctgtaca
    11281 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
    11341 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
    11401 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
    11461 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
    11521 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
    11581 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
    11641 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
    11701 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
    11761 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
    11821 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
    11881 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggcg
    11941 gtggcggagg gatggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca
    12001 aggatgtatt tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga
    12061 tcgtgtacag aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc
    12121 ttactaagcc agatgtgatc ctccggttgg agaagggaga agagccctct ggcggctcaa
    12181 aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gcggaaagtc tgagatcttt
    12241 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
    12301 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
    12361 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
    12421 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
    12481 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
    12541 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
    12601 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
    12661 tccctcttct cttatggaga tccctcgacg gatcggccgc aattcgtaac catgtcatag
    12721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    12781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    12841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    12901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    12961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    13021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    13081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    13141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    13201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    13261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    13321 tgtaggtatc tcagttcggt gtgggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    13381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    13441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    13501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    13561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    13621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    13681 acgcgcagaa aaaaaggatc tcaa
//
