LOCUS       Exported                6572 bp ds-DNA     circular SYN 15-FEB-2024
DEFINITION  pcDNA3.1Star-Mas1-BamHI and EcoRI-HA tag-N-ter-FINAL 6572bp ds-DNA 
            circular SYN  20-FEB-2022.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA 3.1-Mas1-HA tag N-termiuns
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 2, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(56..74)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    100..324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      370..798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        812..1142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      993..1128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1209..2003
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2177..2298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2347..2363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2371..2387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2395..2425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2440..2461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2749..3337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3508..4368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4369..4473)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4739..5118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5119..5322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        5367..5385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5420..5446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat
       61 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      301 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      361 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt
      541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      721 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac
      781 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      841 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      901 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      961 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1021 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1081 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1141 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     1201 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     1261 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     1321 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     1381 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     1441 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     1501 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     1561 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     1621 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     1681 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     1741 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1801 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1861 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     1921 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     1981 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     2041 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     2101 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     2161 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2281 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     2341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4501 cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct
     4561 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     4621 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     4681 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     4741 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4801 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4861 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4921 ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa
     4981 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5041 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5101 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     5161 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5221 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     5281 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     5341 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
     5401 taccgagctc ggatccatgt acccatacga tgttccagat tacgctatgg atgggtcaaa
     5461 cgtgacatca tttgttgttg aggaacccac gaacatctca actggcagga acgcctcagt
     5521 cgggaatgca catcggcaaa tccccatcgt gcactgggtc attatgagca tctccccagt
     5581 ggggtttgtt gagaatggga ttctcctctg gttcctgtgc ttccggatga gaagaaatcc
     5641 cttcactgtc tacatcaccc acctgtctat cgcagacatc tcactgctct tctgtatttt
     5701 catcttgtct atcgactatg ctttagatta tgagctttct tctggccatt actacacaat
     5761 tgtcacatta tcagtgactt ttctgtttgg ctacaacacg ggcctctatc tgctgacggc
     5821 cattagtgtg gagaggtgcc tgtcagtcct ttaccccatc tggtaccgat gccatcgccc
     5881 caagtaccag tcggcattgg tctgtgccct tctgtgggct ctttcttgct tggtgaccac
     5941 catggagtat gtcatgtgca tcgacagaga agaagagagt cactctcgga atgactgccg
     6001 agcagtcatc atctttatag ccatcctgag cttcctggtc ttcacgcccc tcatgctggt
     6061 gtccagcacc atcttggtcg tgaagatccg gaagaacacg tgggcttccc attcctccaa
     6121 gctttacata gtcatcatgg tcaccatcat tatattcctc atcttcgcta tgcccatgag
     6181 actcctttac ctgctgtact atgagtattg gtcgaccttt gggaacctac accacatttc
     6241 cctgctcttc tccacaatca acagtagcgc caaccctttc atttacttct ttgtgggaag
     6301 cagtaagaag aagagattca aggagtcctt aaaagttgtt ctgaccaggg ctttcaaaga
     6361 tgaaatgcaa cctcggcgcc agaaagacaa ttgtaatacg gtcacagttg agactgtcgt
     6421 ctaagaactg tgagggaagt tgtggataaa aatggtggaa cacaggtcat ttttagtttg
     6481 tgcttggaat atgacttaag tatctcctaa atgtgataca gaagaacatc tcatcccata
     6541 tgcatgagat actaattaat gatgaaagaa tt
//
