LOCUS       Exported                5987 bp ds-DNA     circular SYN 04-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   pSEVA121
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 5, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5605..5797
                     /organism="Enterobacteria phage lambda"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10710"
     source          5798..5871
                     /organism="Enterobacteria phage lambda"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10710"
     misc_feature    1..8
                     /label=PacI
     promoter        18..52
                     /label=P-J23100
                     /note="P-J23100"
     RBS             64..90
                     /label=TIR high
                     /note="TIR"
                     /note="high TIR"
     CDS             82..137
                     /codon_start=1
                     /label=BCD
                     /translation="MKAIFVLKHLNHAKEVF"
     CDS             138..848
                     /codon_start=1
                     /product="green fluoresent protein"
                     /label=GFP
                     /note="GFP"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      852..900
                     /label=L3S3P22 Terminator (synthetic)
                     /note="L3S3P22 Terminator"
     promoter        911..939
                     /label=Promoter 3BCD2
                     /note="Promoter 3BCD2"
     misc_feature    973..987
                     /label=TIR RK2
     CDS             979..2169
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="TcR"
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     terminator      2180..2220
                     /label=rrnC
                     /note="rrnC"
     CDS             complement(2224..2850)
                     /codon_start=1
                     /label=cat
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQ"
     promoter        2851..2953
                     /label=cat promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    2971..2976
                     /label=SpeI
     terminator      2977..3079
                     /label=Lambda T0 terminator
                     /label=T0
     misc_feature    3080..3086
                     /label=SanDI
     misc_feature    3087..3097
                     /label=scar1
     misc_feature    3098..3105
                     /label=SwaI
     misc_feature    3106..3115
                     /label=PshAI
                     /note="modified"
     misc_feature    3116..3361
                     /label=OriT
     misc_feature    3362..3369
                     /label=FseI
     rep_origin      3370..5591
                     /label=RK2
     misc_feature    5592..5599
                     /label=AscI
     misc_feature    5605..5871
                     /label=cos
     misc_feature    5786..5797
                     /label=cosN site
     misc_feature    5822..5841
                     /label=ONR455
     misc_feature    5852..5871
                     /label=ONR454
     terminator      complement(5882..5986)
                     /label=T1 terminator
ORIGIN
        1 ttaattaagc gttgcgcttg acggctagct cagtcctagg tacagtgcta gctactagag
       61 attaaagagg agaaactata tatgaaagca attttcgtac tgaaacatct taatcatgct
      121 aaggaggttt tctaatgagc aaaggagaag aacttttcac tggagttgtg ccaattcttg
      181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      241 atgctacata cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
      301 catggccaac acttgtcact actctgacct atggcgttca atgcttttcc cgttatccgg
      361 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
      421 gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag
      481 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
      541 ttctcggaca caaactagag tacaactata actcacacaa tgtatacatc acggcagaca
      601 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggttccg
      661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      721 cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg
      781 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
      841 tctacaaatg accaattatt gaaggccgct aacgcggcct ttttttgttt ctggtctccc
      901 aaaaatttat ttgcttatta atcatccggc tcgtataatg tgtggagggc ccaagttcac
      961 ttaaaaagga gagcgacgat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     1021 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     1081 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     1141 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     1201 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     1261 gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     1321 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     1381 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     1441 ggcgccatct ccctgcacgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     1501 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgtcc gatgcccttg
     1561 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgaccat tgtggccgca
     1621 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     1681 attttcggcg aggaccgctt tcgctggagc gcgacgatga gcggcctgtc gcttgcggta
     1741 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     1801 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     1861 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     1921 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     1981 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     2041 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     2101 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     2161 tcgacctgag acaattgtcc agataaaaaa aatccttagc tttcgctaag gatgatttct
     2221 ttattgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     2281 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     2341 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     2401 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     2461 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     2521 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2581 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2641 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2701 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2761 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2821 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2881 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2941 tacgtgccga tcaacgtctc attttcgcca actagtctgg attctcacca ataaaaaacg
     3001 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3061 ctatcaacag gagtccaagg ggtccccaat aattacgatt taaatgacaa gggtcctttt
     3121 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     3181 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     3241 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     3301 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt
     3361 aggccggccg cgatgcaggt ggctgctgaa cccccagccg gaactgaccc cacaaggccc
     3421 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
     3481 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
     3541 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
     3601 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
     3661 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
     3721 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
     3781 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
     3841 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
     3901 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
     3961 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
     4021 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
     4081 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
     4141 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
     4201 agcaacgcgg cctgcttggc ttcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
     4261 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
     4321 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
     4381 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac tatcgagccg
     4441 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
     4501 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
     4561 cgattcattc accctccttg cgggattgcc ccggaattaa ttccccggat cgatccgtcg
     4621 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     4681 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     4741 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     4801 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     4861 accgtttctg cggactggct ttctacgtgg ctgccatttt tggggtgagg ccgttcgcgg
     4921 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     4981 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     5041 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     5101 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     5161 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     5221 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     5281 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     5341 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     5401 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     5461 ttttccgcga ggtatccaca acgccggcgg ccctacatgg ctctgctgta gtgagtgggt
     5521 tgcgctccgg cagcggtcct gatcccccgc agaaaaaaag gatctcaaga agatcctttg
     5581 atcttttcta cggcgcgccc aataccattg ttcattccac ggacaaaaac agagaaagga
     5641 aacgacagag gccaaaaagc ctcgctttca gcacctgtcg tttcctttct tttcagaggg
     5701 tattttaaat aaaaacatta agttatgacg aagaagaacg gaaacgcctt aaaccgaaaa
     5761 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa
     5821 aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgtg gattacgatt
     5881 ttctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     5941 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
