LOCUS       Exported                4874 bp ds-DNA     circular SYN 04-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   pSEVA121
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 5, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..8
                     /label=PacI
     promoter        18..52
                     /label=P-J23100
                     /note="P-J23100"
     RBS             64..90
                     /label=TIR high
                     /note="TIR"
                     /note="high TIR"
     CDS             82..137
                     /codon_start=1
                     /label=BCD
                     /translation="MKAIFVLKHLNHAKEVF"
     CDS             138..848
                     /codon_start=1
                     /product="green fluoresent protein"
                     /label=GFP
                     /note="GFP"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      852..900
                     /label=L3S3P22 Terminator (synthetic)
                     /note="L3S3P22 Terminator"
     promoter        911..939
                     /label=Promoter 3BCD2
                     /note="Promoter 3BCD2"
     misc_feature    973..987
                     /label=TIR high copy
     CDS             979..2169
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="TcR"
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     terminator      2180..2220
                     /label=rrnC
                     /note="rrnC"
     CDS             complement(2224..2850)
                     /codon_start=1
                     /label=cat
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQ"
     promoter        2851..2953
                     /label=cat promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    2971..2976
                     /label=SpeI
     terminator      2977..3079
                     /label=Lambda T0 terminator
                     /label=T0
     misc_feature    3080..3086
                     /label=SanDI
     misc_feature    3098..3105
                     /label=SwaI
     misc_feature    3106..3115
                     /label=PshAI
                     /note="modified"
     misc_feature    3116..3361
                     /label=OriT
     misc_feature    3362..3369
                     /label=FseI
     rep_origin      3395..3983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4169..4309
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4411..4602)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4755..4762
                     /label=AscI
     terminator      4763..4874
                     /label=T1
ORIGIN
        1 ttaattaagc gttgcgcttg acggctagct cagtcctagg tacagtgcta gctactagag
       61 attaaagagg agaaactata tatgaaagca attttcgtac tgaaacatct taatcatgct
      121 aaggaggttt tctaatgagc aaaggagaag aacttttcac tggagttgtg ccaattcttg
      181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      241 atgctacata cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
      301 catggccaac acttgtcact actctgacct atggcgttca atgcttttcc cgttatccgg
      361 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
      421 gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag
      481 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
      541 ttctcggaca caaactagag tacaactata actcacacaa tgtatacatc acggcagaca
      601 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggttccg
      661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      721 cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg
      781 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
      841 tctacaaatg accaattatt gaaggccgct aacgcggcct ttttttgttt ctggtctccc
      901 aaaaatttat ttgcttatta atcatccggc tcgtataatg tgtggagggc ccaagttcac
      961 ttaaaaagga gattggacat gaagtccaac aatgcgctca tcgtcatcct cggcaccgtc
     1021 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     1081 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     1141 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     1201 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     1261 gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     1321 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     1381 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     1441 ggcgccatct ccctgcacgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     1501 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgtcc gatgcccttg
     1561 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgaccat tgtggccgca
     1621 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     1681 attttcggcg aggaccgctt tcgctggagc gcgacgatga gcggcctgtc gcttgcggta
     1741 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     1801 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     1861 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     1921 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     1981 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     2041 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     2101 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     2161 tcgacctgag acaattgtcc agataaaaaa aatccttagc tttcgctaag gatgatttct
     2221 ttattgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     2281 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     2341 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     2401 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     2461 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     2521 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2581 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2641 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2701 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2761 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2821 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2881 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2941 tacgtgccga tcaacgtctc attttcgcca actagtctgg attctcacca ataaaaaacg
     3001 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3061 ctatcaacag gagtccaagg ggtccccaat aattacgatt taaatgacaa gggtcctttt
     3121 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     3181 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     3241 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     3301 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt
     3361 aggccggccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4141 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     4201 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4261 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4321 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4381 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4441 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc
     4501 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     4561 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     4621 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     4681 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     4741 atggatgcgg cgggggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga
     4801 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     4861 tttatttgat gcct
//
