LOCUS       pSS1-mNeongreen         4131 bp ds-DNA     circular     07-JUN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        7..25
                     /label="T3 promoter"
     misc_feature    39..184
                     /label="PmMsg Promoter"
     misc_feature    199..204
                     /label="Kozak"
     misc_feature    205..915
                     /label="mNeongreen (CO-Sp)"
     misc_feature    929..1079
                     /label="PmNamp8 terminator"
     primer_bind     complement(1096..1115)
                     /label="T7"
     rep_origin      complement(1326..1914)
                     /label="ori"
     CDS             complement(2081..2872)
                     /label="AmpR"
     CDS             complement(2873..2941)
                     /label="AmpR"
     CDS             complement(2959..3753)
                     /label="NeoR/KanR"
ORIGIN
        1 GCTAGCAATT AACCCTCACT AAAGGGAGAC TCTACGTCAA TTGCACAAAT TTGAGAAAAG
       61 AGACGGATAT CAAGAAAAAT ATATTTTTCA AACGCTAAAA CAAATAACGG GTAAAGCAAA
      121 TACACAGTCC GGAAATAACC CCTTTAATAC GAAAATAATA ATTAACATTC TCATAAACTC
      181 CCGATCTGAC CTAAGGTCAA AAAAATGGTT TCAAAGGGCG AAGAGGATAA CATGGCCTCT
      241 CTCCCAGCGA CACATGAGTT ACACATATTT GGCTCTATTA ACGGTGTTGA TTTTGACATG
      301 GTCGGTCAAG GAACGGGTAA TCCAAATGAT GGTTATGAAG AGTTAAACCT GAAAAGCACT
      361 AAGGGAGATC TTCAATTCTC TCCTTGGATT TTAGTCCCTC ATATCGGGTA TGGCTTTCAT
      421 CAGTATCTTC CCTACCCTGA CGGTATGAGT CCTTTTCAGG CAGCCATGGT AGATGGATCC
      481 GGATACCAAG TACATCGAAC AATGCAATTT GAAGATGGTG CCTCCCTTAC TGTTAATTAT
      541 AGATATACCT ACGAAGGAAG CCACATTAAA GGAGAAGCTC AAGTTAAGGG GACTGGTTTT
      601 CCTGCTGACG GTCCTGTGAT GACTAATTCT TTGACCGCTG CTGACTGGTG TAGGTCGAAG
      661 AAAACTTACC CCAACGATAA AACGATAATT AGTACCTTTA AATGGTCATA TACTACTGGA
      721 AATGGTAAAC GCTACCGTTC AACTGCTAGA ACCACATATA CTTTTGCAAA ACCAATGGCT
      781 GCTAATTATT TGAAGAATCA GCCGATGTAT GTGTTCCGTA AAACGGAATT GAAGCATTCT
      841 AAGACAGAAC TAAATTTCAA AGAATGGCAA AAAGCATTTA CAGATGTTAT GGGCATGGAT
      901 GAGTTATATA AATAAGGTGA GTGACACTAC GACGATAAGG AGAATGTACT GGATTTTGAT
      961 AAGCGTTGTT ATCTTGATTA TTTACTTCAA TTTATTTTTT GTTTTTATAT TTGTTTTTAA
     1021 TATTGTATTC TAGTCTTTAA ACTGTTTATT TAAATAGTAT TGCCTTAAAG CAATACGTAG
     1081 AATGCTACTC ACCCTCCCTA TAGTGAGTCG TATTAGGCCA CAATCGTGAA TGTGTTTGCG
     1141 AATTTAGAGG TATGTATTTT ATCAGATTAT TTTACTATAA CATTCATAAT CAAATTATAT
     1201 TTTTAAAATA TTACTTCAAG TGTATATCAT GTATAATTAC ACGGCATACA AAAAAAAAAC
     1261 TCGTCAATAA ATAAATAGAA TTTATTTTAT TAACTACAGG AATGTGATGG CCGCGTTGCT
     1321 GGCGTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     1381 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     1441 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     1501 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     1561 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     1621 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     1681 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     1741 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     1801 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     1861 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     1921 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1981 TTGGTCATGA GATTATCAAA GAAGATCTTC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2041 AAATCAATCT AAAGTATATA TGAGTAACCG GTTCTGACAG CACCCAGAAA CGCTGGTCAA
     2101 AGTAAAAGAT GCTGAAGACC AGTTGGGTGC GCGGGTGGGT TACATCGAAC TGGATCTCAA
     2161 TAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTCCCAATGA TGAGCACCTT
     2221 TAAAGTTCTG CTGTGTGGCG CGGTGTTATC CCGTATTGAC GCCGGTCAAG AGCAGCTCGG
     2281 TCGCCGCATT CACTATTCGC AGAATGACTT GGTTGAATAT AGTCCGGTCA CAGAAAAGCA
     2341 TCTGACGGAT GGCATGACAG TCAGAGAACT GTGCTCAGCT GCCATAACCA TGAGCGATAA
     2401 CACTGCGGCC AACTTACTTC TGACCACGAT CGGAGGACCG AAAGAACTGA CCGCCTTCTT
     2461 GCACAACATG GGGGATCATG TGACTCGCTT AGATCGTTGG GAACCGGAGC TGAATGAAGC
     2521 GATACCGAAC GACGAGCGTG ACACCACGAT GCCTGTGGCA ATGGCAACAA CCTTGCGCAA
     2581 ACTATTAACT GGCGAACTTC TGACTCTCGC TTCCCGGCAA CAACTGATTG ACTGGATGGA
     2641 AGCGGATAAA GTTGCAGGAC CTCTGCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC
     2701 GGATAAATCT GGCGCCGGGG AGCGTGGCTC TCGCGGTATT ATTGCAGCGC TGGGGCCGGA
     2761 TGGTAAACCC AGTCGTATCG TAGTGATCTA CACGACCGGC AGCCAGGCAA CCATGGATGA
     2821 ACGAAATAGG CAGATCGCGG AGATTGGCGC CTCACTGATT AAGCATTGGT AAAGCAAAAA
     2881 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
     2941 TACTCTTCCT TTTTCAATTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG
     3001 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG
     3061 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG
     3121 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA
     3181 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC
     3241 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG
     3301 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG
     3361 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT
     3421 CTCGGCAGGA GCAAGGTGGG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG
     3481 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT
     3541 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCAGGGCAC CGGACAGGTC
     3601 GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA
     3661 GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG
     3721 AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG
     3781 ATCAGATCTT GATCCCCTGC GCCATCAGAT CCTTGGCGGC AAGAAAGCCA TCCAGTTTAC
     3841 TTTGCAGGGC TTCCCAACCT TACCAGAGGG CGCCCCAGCT GGCAATTCCG GTTCGCTTGC
     3901 TGTCCATAAA ACCGCCCAGT CTAGCTATCG CCATGTAAGC CCACTGCAAG CTACCTGCTT
     3961 TCTCTTTGCG CTTGCGTTTT CCCTTGTCCA GATAGCCCAG TAGCTGACAT TCATCCGGGG
     4021 TCAGCACCGT TTCTGCGGAC TGGCTTTCTA CGTGTTCCGC TTCCTTTAGC AGCATGAATC
     4081 AGAAATTTGG GGATAATGGG AATTTTTTAC TATATACAAA GGACCAGCTC A
//