LOCUS       pSS2.1-mNeongreen       6031 bp ds-DNA     circular     07-JUN-2025
DEFINITION  .
ACCESSION   VB241118-2903may
VERSION     VB241118-2903may
COMMENT     Imported using the Genbank importer. File name:
            pSS2.1-mNeongreen.gb
FEATURES             Location/Qualifiers
     Promoter        7..25
                     /label="T3 promoter"
                     /note="color: #0375bb; direction: RIGHT"
                     /References="<a href= target=_blank>Gene Expr Patterns.9:54 (2009)</a>; <a href= target=_blank>Biotechnol Lett.33:1319 (2011)</a>"
                     /Subtype="Zebrafish ubiquitous promoter"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="T3 promoter"
                     /official_designation="T3 promoter"
                     /uuid="23b077fdf0b0405fb778fb2619a95978"
     misc_feature    39..184
                     /label="PmMsg Promoter"
                     /note="color: #a949ca; direction: RIGHT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="PmMsg Promoter"
                     /official_designation="PmMsg Promoter"
                     /uuid="6ff531ea9f524266ae385d6952c92e3b"
     misc_feature    205..915
                     /label="mNeongreen (CO-Sp)"
                     /note="color: #946a00; direction: RIGHT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="mNeongreen (CO-Sp)"
                     /official_designation="mNeongreen (CO-Sp)"
                     /uuid="247c64d7da144e549da26f8cedd96ec1"
     misc_feature    929..1079
                     /label="PmNamp8 terminator"
                     /note="color: #512bbd; direction: RIGHT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="PmNamp8 terminator"
                     /official_designation="PmNamp8 terminator"
                     /uuid="cfc00fe9b1804b54838215ef481f54e6"
     Promoter        complement(1097..1115)
                     /label="T7 promoter"
                     /note="color: #d84e4e; direction: LEFT"
                     /Application=""
                     /Full=""
                     /References="Nucleic Acids Res. 7:1931 (1979); J Mol Biol.219:45 (1991)"
                     /Subtype="E. coli promoter"
                     /__SeqFeature__="True"
                     /__level__=0
                     /description="T7 promoter"
                     /name="T7 promoter"
                     /official_designation="T7 promoter"
                     /uuid="97321cbbef8d45c48efed8c0d548c338"
     Rep_origin      complement(1326..1914)
                     /label="pUC ori"
                     /note="color: #1f36a9; direction: LEFT"
                     /Application=""
                     /Full=""
                     /References="Mol Microbiol. 6:3385 (1992)"
                     /Subtype="E. coli replication_origin"
                     /__SeqFeature__="True"
                     /__level__=0
                     /application_notes="Facilitates plasmid replication in E.coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /name="pUC ori"
                     /official_designation="pUC ori"
                     /uuid="f09c4dacdc56460eac9fa1805e592776"
     misc_feature    complement(2018..2518)
                     /label="TEF1 terminator"
                     /note="color: #db6901; direction: LEFT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="TEF1 terminator"
                     /official_designation="TEF1 terminator"
                     /uuid="5397093aa164406aa3db747b84a32bba"
     misc_feature    complement(2519..2941)
                     /label="Bsr"
                     /note="color: #800c0c; direction: LEFT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="Bsr"
                     /official_designation="Bsr"
                     /uuid="64fa4d9ad8c54f40897b13c8a411c6ac"
     misc_feature    complement(2942..3385)
                     /label="TEF1 promoter"
                     /note="color: #0c5d90; direction: LEFT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="TEF1 promoter"
                     /official_designation="TEF1 promoter"
                     /uuid="f4dd31ce461e402cabbef5707b938f80"
     CDS             complement(3434..4225)
                     /label="AmpR"
     CDS             complement(4226..4294)
                     /label="AmpR"
     CDS             complement(4312..5106)
                     /label="NeoR/KanR"
     CDS             complement(4312..5106)
                     /label="NeoR/KanR"
                     /note="color: #f58600; direction: LEFT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /name="NeoR/KanR"
                     /official_designation="NeoR/KanR"
                     /uuid="af8fa7c3efea4a0dbcd43d7e771a54d2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    5260..5811
                     /label="Pmcen15"
                     /note="color: #5566f5; direction: RIGHT"
                     /__SeqFeature__="True"
                     /__level__=0
                     /direction="NONE"
                     /name="Pmcen15"
                     /official_designation="Pmcen15"
                     /uuid="ba635dcaaf9b448aa7f427a243c2f534"
ORIGIN
        1 gctagcaatt aaccctcact aaagggagac tctacgtcaa ttgcacaaat ttgagaaaag
       61 agacggatat caagaaaaat atatttttca aacgctaaaa caaataacgg gtaaagcaaa
      121 tacacagtcc ggaaataacc cctttaatac gaaaataata attaacattc tcataaactc
      181 ccgatctgac ctaaggtcaa aaaaatggtt tcaaagggcg aagaggataa catggcctct
      241 ctcccagcga cacatgagtt acacatattt ggctctatta acggtgttga ttttgacatg
      301 gtcggtcaag gaacgggtaa tccaaatgat ggttatgaag agttaaacct gaaaagcact
      361 aagggagatc ttcaattctc tccttggatt ttagtccctc atatcgggta tggctttcat
      421 cagtatcttc cctaccctga cggtatgagt ccttttcagg cagccatggt agatggatcc
      481 ggataccaag tacatcgaac aatgcaattt gaagatggtg cctcccttac tgttaattat
      541 agatatacct acgaaggaag ccacattaaa ggagaagctc aagttaaggg gactggtttt
      601 cctgctgacg gtcctgtgat gactaattct ttgaccgctg ctgactggtg taggtcgaag
      661 aaaacttacc ccaacgataa aacgataatt agtaccttta aatggtcata tactactgga
      721 aatggtaaac gctaccgttc aactgctaga accacatata cttttgcaaa accaatggct
      781 gctaattatt tgaagaatca gccgatgtat gtgttccgta aaacggaatt gaagcattct
      841 aagacagaac taaatttcaa agaatggcaa aaagcattta cagatgttat gggcatggat
      901 gagttatata aataaggtga gtgacactac gacgataagg agaatgtact ggattttgat
      961 aagcgttgtt atcttgatta tttacttcaa tttatttttt gtttttatat ttgtttttaa
     1021 tattgtattc tagtctttaa actgtttatt taaatagtat tgccttaaag caatacgtag
     1081 aatgctactc accctcccta tagtgagtcg tattaggcca caatcgtgaa tgtgtttgcg
     1141 aatttagagg tatgtatttt atcagattat tttactataa cattcataat caaattatat
     1201 ttttaaaata ttacttcaag tgtatatcat gtataattac acggcataca aaaaaaaaac
     1261 tcgtcaataa ataaatagaa tttattttat taactacagg aatgtgatgg ccgcgttgct
     1321 ggcgttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1921 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1981 ttggtcatga gattatcaaa gaagatcttc tagatccagg tacgacatta tacttgttat
     2041 gttttacaaa tgaattggta acaacacttg aatttatcaa aaaatatcca tcaattattt
     2101 ctgatattct tatttatggt tgttctggag caattggaca attgtttatt ttccatacat
     2161 tagaaaaata tggcagttta atcttaataa ctatcacact tacaagaaaa atgctaacgc
     2221 tattattaag tcttatttgg tttaatcata agttaacaat tggccagtgg ataggtgtag
     2281 tattggtatt ttatggagca agtttggaag catatataaa acatacacaa ttagctaaaa
     2341 aaaaagtata aaacatttat acgaaatctc atacgtaaat ttataaaatt tataataaac
     2401 atataaataa tataaaaatg aaacaatatt ctaaaaacaa atctataaca aaaaacatat
     2461 attttaatta cgctacataa ttaatatata atagcaatac catcaatata ctgcttcctt
     2521 aattacgagt atacttaaga ggtatcaatt cctcaatagt tgttttaaca agtttaccat
     2581 tcatctcaat aagaacaaaa caatctggcg cataatctga tataagctct cgacacatac
     2641 cacatggaga aacaacacga atagacctat caacttcatc agaataagga tgtcgaactg
     2701 caacaatagt atcaaaatcc ttttgtccat tagatactgc acttccaata gctattgcct
     2761 ctgcacaaac agtaacacgt cctatataag cttcaatgtg aacagctgat ataatctctc
     2821 cagtctttgt tctaattgct gctccaacat ggtgtttatt atcctcataa agcattgtaa
     2881 tcttttctgt agcaacttct actaattcta aatcctgctg agatatatta aatgtcttca
     2941 tctttttttt aatatatttt tttcaaatat ctaaatatta ttttatgtta aaatgaattt
     3001 ttgaacgtcg atattcaaaa aatatcatga ctgtttttat ttttttgacc taattttttc
     3061 agattaagaa taaatattta acattaagat ttttagatta tacaataaag acgtcatttc
     3121 ttatgacaac tagtccaaat aactacataa ttataaaaat tattagaaaa atctacgttt
     3181 ctaaaaccaa ttcaatatta tatatatata tatatagcct tcataattat tctagaaaac
     3241 aatttatttt aatactattc ttttaaagct acaatattca atattatcat ctatatcatt
     3301 attattacat atctttaata tttttcagaa aaataattat aaaaataaaa cgtgccatca
     3361 tattttttat tgaaattaaa tctatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3421 ccggttctga cagcacccag aaacgctggt caaagtaaaa gatgctgaag accagttggg
     3481 tgcgcgggtg ggttacatcg aactggatct caatagcggt aagatccttg agagttttcg
     3541 ccccgaagaa cgtttcccaa tgatgagcac ctttaaagtt ctgctgtgtg gcgcggtgtt
     3601 atcccgtatt gacgccggtc aagagcagct cggtcgccgc attcactatt cgcagaatga
     3661 cttggttgaa tatagtccgg tcacagaaaa gcatctgacg gatggcatga cagtcagaga
     3721 actgtgctca gctgccataa ccatgagcga taacactgcg gccaacttac ttctgaccac
     3781 gatcggagga ccgaaagaac tgaccgcctt cttgcacaac atgggggatc atgtgactcg
     3841 cttagatcgt tgggaaccgg agctgaatga agcgataccg aacgacgagc gtgacaccac
     3901 gatgcctgtg gcaatggcaa caaccttgcg caaactatta actggcgaac ttctgactct
     3961 cgcttcccgg caacaactga ttgactggat ggaagcggat aaagttgcag gacctctgct
     4021 gcgctcggcc cttccggctg gctggtttat tgcggataaa tctggcgccg gggagcgtgg
     4081 ctctcgcggt attattgcag cgctggggcc ggatggtaaa cccagtcgta tcgtagtgat
     4141 ctacacgacc ggcagccagg caaccatgga tgaacgaaat aggcagatcg cggagattgg
     4201 cgcctcactg attaagcatt ggtaaagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa ttcagaagaa
     4321 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     4381 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     4441 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     4501 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     4561 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     4621 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     4681 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     4741 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gggatgacag
     4801 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     4861 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     4921 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     4981 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     5041 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     5101 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca
     5161 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     5221 gggcgcccca gctggcaatt ccggttcgct tgctgtccat agtagttcta gaatttttgt
     5281 taaaatttta tttcaagagt tgatgggagt tcttggtata cgcgaattag atgaacgttt
     5341 gaaagatcca cttttacaag agactctttc taatatgttt cctcgggata atccaaaaaa
     5401 tactagattt gcaataaatt attttacttc tattggatta ggattgataa ctgtttcatt
     5461 gcgtgagcat ttggaaacta tcaaggtaaa gctttgaaaa tatagtatta actctaattt
     5521 ctttagaata ctgttacagt aaatcgaagt ctttctaaat cttcatcatc ttcaattata
     5581 tcttcctata ctacatcata ttcatctgaa tctttatcat atcccaatga tacttcagag
     5641 tcagaatcat tgaatcgttc aagatctcgt catcggtcac ctgctcgtca tagaatgaga
     5701 tcccctcgag ataatcgttc aaggccacgt tcatgttcat taaatcgttc tagattgcaa
     5761 tcttctgagt cggaatcatt gaatcgttcg agatctcgtc atcggtcacc tccgcccagt
     5821 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     5881 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     5941 tggctttcta cgtgttccgc ttcctttagc agcatgaatc agaaatttgg ggataatggg
     6001 aattttttac tatatacaaa ggaccagctc a
//