LOCUS       CH.1.1                  7383 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  CH.1.1.
ACCESSION   CH.1.1
VERSION     CH.1.1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(1251..5006)
                     /note="pLannotate"
                     /label="SPIKE_SARS2"
                     /database="swissprot"
                     /identity="96.7"
                     /match_length="98.4"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Attaches the virion to the cell
                     membrane by interacting with host receptor, initiating the
                     infection. The major receptor is host ACE2
                     (PubMed:32142651, PubMed:32155444, PubMed:33607086). When
                     S2/S2' has been cleaved, binding to the receptor triggers
                     direct fusion at the cell membrane (PubMed:34561887). When
                     S2/S2' has not been cleaved, binding to the receptor
                     results in internalization of the virus by endocytosis
                     using host TFRC and GRM2 and leading to fusion of the
                     virion membrane with the host endosomal membrane
                     (PubMed:32075877, PubMed:32221306, PubMed:34903715,
                     PubMed:36779763). Alternatively, may use NRP1/NRP2
                     (PubMed:33082294, PubMed:33082293) and integrin as entry
                     receptors (PubMed:35150743). The use of NRP1/NRP2 receptors
                     may explain the tropism of the virus in human olfactory
                     epithelial cells, which express these molecules at high
                     levels but ACE2 at low levels (PubMed:33082293). The stalk
                     domain of S contains three hinges, giving the head
                     unexpected orientational freedom (PubMed:32817270). From
                     Severe acute respiratory syndrome coronavirus 2
                     (2019-nCoV)."
     enhancer        complement(5686..6065)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /description="human cytomegalovirus immediate early
                     enhancer"
     CDS             6353..7213
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     polyA_signal    complement(1004..1228)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="bovine growth hormone polyadenylation signal"
     promoter        complement(5482..5685)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="human cytomegalovirus (CMV) immediate early
                     promoter"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     promoter        6248..6352
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     LTR             complement(5166..5393)
                     /note="pLannotate"
                     /label="5' LTR (truncated) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="84.8"
                     /fragment="True"
                     /other="LTR"
                     /description="truncated 5' long terminal repeat (LTR) from
                     human T-cell leukemia virus (HTLV) type 1"
     intron          complement(5052..5162)
                     /note="pLannotate"
                     /label="CMV intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.1"
                     /fragment="True"
                     /other="intron"
                     /description="modified intron A from human cytomegalovirus"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttccgat
      661 tggccttaaa tcatggcctt aaaattctat gatttctctc attacttcct cacatgttgg
      721 aggcattttc tctccctctg cacttaatag cctatcttgc tttaatttct tcccactctt
      781 ggaggctagg tttggtttgg tgggctgatg agggagggag agaccgctcc aagtacttta
      841 gcgggtggga ttgaaggcgg agccctcctg agctatgagt gtcctatgag tggggctgga
      901 actaagaacc aggggcgtgg acagggtgtg tcacagagaa ggggatgtgc ctgcttcttt
      961 ctggcccagg aggaaccggg tcaattcttc agcacctggg tacccataga gcccaccgca
     1021 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     1081 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     1141 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     1201 acagatggct ggcaactaga aggcacagca gatctggatc ctcatcatta tttacagcaa
     1261 gagccgcagc tacaacagcc cttgaggcag ctgcagcagc tagtcataca acaaagcatg
     1321 atggtcacca tgacgatagc gatcaggcct gctataaagc ccagccaaat gtaccaaggc
     1381 catttaatgt actgctcata ttttcccagc tcctgcaggt caataagtga ttcgttgagg
     1441 ttcttggcca cttcgttaag tctatcaatc tctttctgga tgttcaccac gctagcatta
     1501 attcctgaaa tgtcgccgag gtcaacatcg ggtgaggtgt gattcttaaa gtacttatcc
     1561 agttcctcct tgaaagagtc aagttccggt tggagaggat cgtacacggt gttgttcacg
     1621 atcccaataa ctacgtcaca gttcccagat acgaatgtgt tgtcggttgt gattatctgg
     1681 ggctcataaa agttcctctg ggtaacaaac cagtgtgttc cattgctaac aaagacgcct
     1741 tcgcgtggaa agtgtgcctt accgtcgtgg cagatcgccg gagcggtagt aaagttcttc
     1801 tcttgagcgg gaacgtaagt aacatgcagg aacacaacgc catgtggggc ggattggggg
     1861 aagctcatga ggtggtaccc cttcccacaa aagtcgaccc gctttgattg cccgagcacg
     1921 cattcactca tctttgtagc agccagattc gcagatgctc taatctcggc ggctctgata
     1981 agctgctgcg tgacgtacgt ctgcagactc tgcagccttc cagtaataag ccggtcgatc
     2041 tggacttctg cctccacctt atccaatcta ctaaggatat cattcagcac ggaggatata
     2101 gcaccgaact tggatgacag ttgcttgacg agagtattca gggcttgtgc attgtgattg
     2161 actacatctt gcaatttccc gagggcgctg gctgtggaac tcaagctgtc ctggatttta
     2221 ccaatggcgg aattgaactg gttagcgata agtttctggt tttcgtacag gacgttctgg
     2281 gtcaccccga tcccattgaa gcgatatgcc atctgcatgg caaaaggtat ttgcagtgcg
     2341 gctcctgctc cgaacgtcca gccggatgta atagtgccgg ccagcagggc gctggtatat
     2401 tgtgcgatca tctcatcagt gagcagcggt gggagtacgg tcagcccgtt gaatttctgg
     2461 gcacatatca gatcgcgagc agcaatgtct cccaaacagt cgccatactg cttaataaat
     2521 ccagcatcgg cgagggtcac cttattaaag agcaaatcct ctataaagct ccgcttagat
     2581 ggcttagaag gatcaggcag aatttgagag aaattgaacc caccgaagta cttgattggt
     2641 ggcgtcttgt aaatctgctt aacctgcgca aacacctcct gagtgttctt atcctgttcg
     2701 acggcgattc ctgtcagtgc cctcttcagc tgtgtgcaga agctgccgta ctgcaggagc
     2761 aggttggaac attcggtgct atcaccacag atgtacatgg tacagtccac ggaggtttta
     2821 gtcatagaca ctggcaggat ctctgtagtc acagagatgg taaaatttgt ggggatagcg
     2881 atagagttgt tactgtatgc cactgaattt tcagccccca gtgacattgt ataggcgatt
     2941 atggactggg aggccacgct cctagccctt ctgtggctct ttgtctgggt ctgataagag
     3001 gcgcagatac ctgctccaat cggtatatca cattcataag agttgttcac gtactcggct
     3061 ccaatgaggc agccggctct ggtctggaat acattagaac cggtgctgta tacccgccat
     3121 gttggggtca gctggtcggc gtgaatagcc acggggacct cggtacagtt tacaccttga
     3181 tacagcacag cgacttgatt tgaggtattt gtgccgggag taataacact cactcctcca
     3241 aaagaacaag gagtaatgtc cagaatctcc aatgtctggg gatctcgcac cgcatcggtc
     3301 gtgtctgcga tatccctgcc gaactgctgg aatggcagga atttcttatt ggattctgtc
     3361 aacacgcctg tcccggtgag gccattgaag ttgaagttca cgcacttatt tttcaccaga
     3421 ttggtagact ttttaggccc acatactgta gcaggcgcat gaagcagctc aaatgacagc
     3481 accaccaccc gatatggctg gtggccaacg ccgtaagtcg gccgaaatcc atagctttgc
     3541 agcgggaagt aacaattgct cccggcgacc ccgttgcagg gcttgtttcc ggcctggtag
     3601 atctctgttg aaatgtcccg ctcaaaaggt ttcagcttgc tctttcgaaa cagcctatac
     3661 cgatagttgt aattgccgct cacggtacta tcgagcttat tggagttcca ggcgatgacg
     3721 cagccggtga aatcatctgg cagtttgtaa ttgtagtcgg ctatgtttcc agtctgccct
     3781 ggggcgattt ggctgacctc gttacctcta ataacgaagc tgtccgcata gacattagta
     3841 aagcacagat cattcagctt cgtaggggag acaccgtagc atttaaaggc gaaaaaggga
     3901 gcgaaattgt acagtacgct gtaatcggcc acgcaattgg atatccgttt cctgttccag
     3961 gcgtaaacac tggcaaaggt agttgcgtta aagacctcgt ggaatgggca caaattggta
     4021 atgttgggga agcgaacgat gctctctgtg ggctgaactc tgaagttgct cgtctggtaa
     4081 atgcccttct cgactgtgaa gcttttcagt gtgcatttgg tttcagacaa tgggtccagc
     4141 gcgcaatcaa cagcgtcagt gatggtgccg ttctcgttgt atttgagcag gaaggtccta
     4201 ggctgcagat atcccacata atatgcagca gcacccgcgg tccagcttga ggatgaatct
     4261 ccaggggtca gataagaccg atgcagtgcg agcagagttt ggaagcgcgt gatgttgatg
     4321 ccgataggaa ggtccacgag gggttccagg gcgctaaaac cttgtggcaa atctctgccc
     4381 aggttcacag gggtgtgctt gctgtatatt ttaaaataac cgtcgatgtt cttgaacacg
     4441 aattcgcgca ggttcttaaa gttcccctgt ttcccttcga ggtccatcag gaaaggctgg
     4501 cttacgtact caaaagtaca gttatttgca ctggagtaca cgcgcagctc actttccatc
     4561 cgagatttat tgttctcatg gtaatacacg tcgaggaaag ggtcgttgca gaactggaac
     4621 tcgcagactt ttatgacgac gtttgtcgca ttattcacga tcagcaggga ttgtgtttta
     4681 ctatccagag tcgtgccgaa gatccatccc cggataatat tgctcttttc tgtactggca
     4741 aaatacacgc cgtcgttaaa aggcaacacg ggattgtcga atctcttagt gccattagtc
     4801 ccggatacgt gtattgcatg aaaccaagtc acatttgaga agaagggcaa gaaaaggtcc
     4861 tgcgtggagt gcaaaacact gcttcggaac accttgtcgg gatagtagac gcctcgtgta
     4921 aaggaattgg tgtagctctg tgttcgcgtg atcaagttga cgcactgact agacaccagg
     4981 ggaagaagaa ccagaaacac gaacattcta gagcggccgc gatatctgat cacacgtgtc
     5041 gacgacggtg actgcagaaa agacccatgg aaaggaacag tctgttagtc tgtcagctat
     5101 tatgtctggt ggcgcgcgcg gcagcaacga gtactgctca gactacactg ccctccaccg
     5161 ttaactagag ttgagcaagc agggtcaggc aaagcgtgga gagccggctg agtctaggta
     5221 ggctccaagg gagcgccgga caaaggcccg gtctcgacct gagctttaaa cttacctaga
     5281 cggcggacgc agttcaggag gcaccacagg cgggaggcgg cagaacgcga ctcaaccggc
     5341 gtggatggcg gcctcaggta gggcggcggg cgcgtgaagg agagatgcga gccgatggag
     5401 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
     5461 gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     5521 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     5581 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     5641 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     5701 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     5761 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     5821 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     5881 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     5941 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     6001 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     6061 atgtcaacat ggcggtaatg ttggacatga gccaatataa atgtacatat tatgatatgg
     6121 atacaacgta tgcaatggcc aatagccaat ctgatgcggt attttctcct tacgcatctg
     6181 tgcggtattt cacaccgcat atggtgcaat cattgggacg tcaggtggca cttttcgggg
     6241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6361 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6421 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6481 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6541 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6601 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6661 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6721 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6781 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6841 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6901 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6961 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7021 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7081 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7141 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7201 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7381 ttc
//
