LOCUS       XBB.1.5                 7380 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  XBB.1.5.
ACCESSION   XBB.1.5
VERSION     XBB.1.5
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(1251..5003)
                     /note="pLannotate"
                     /label="SPIKE_SARS2"
                     /database="swissprot"
                     /identity="96.6"
                     /match_length="98.3"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Attaches the virion to the cell
                     membrane by interacting with host receptor, initiating the
                     infection. The major receptor is host ACE2
                     (PubMed:32142651, PubMed:32155444, PubMed:33607086). When
                     S2/S2' has been cleaved, binding to the receptor triggers
                     direct fusion at the cell membrane (PubMed:34561887). When
                     S2/S2' has not been cleaved, binding to the receptor
                     results in internalization of the virus by endocytosis
                     using host TFRC and GRM2 and leading to fusion of the
                     virion membrane with the host endosomal membrane
                     (PubMed:32075877, PubMed:32221306, PubMed:34903715,
                     PubMed:36779763). Alternatively, may use NRP1/NRP2
                     (PubMed:33082294, PubMed:33082293) and integrin as entry
                     receptors (PubMed:35150743). The use of NRP1/NRP2 receptors
                     may explain the tropism of the virus in human olfactory
                     epithelial cells, which express these molecules at high
                     levels but ACE2 at low levels (PubMed:33082293). The stalk
                     domain of S contains three hinges, giving the head
                     unexpected orientational freedom (PubMed:32817270). From
                     Severe acute respiratory syndrome coronavirus 2
                     (2019-nCoV)."
     enhancer        complement(5683..6062)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /description="human cytomegalovirus immediate early
                     enhancer"
     CDS             6350..7210
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     polyA_signal    complement(1004..1228)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="bovine growth hormone polyadenylation signal"
     promoter        complement(5479..5682)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="human cytomegalovirus (CMV) immediate early
                     promoter"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     promoter        6245..6349
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     LTR             complement(5163..5390)
                     /note="pLannotate"
                     /label="5' LTR (truncated) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="84.8"
                     /fragment="True"
                     /other="LTR"
                     /description="truncated 5' long terminal repeat (LTR) from
                     human T-cell leukemia virus (HTLV) type 1"
     intron          complement(5049..5159)
                     /note="pLannotate"
                     /label="CMV intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.1"
                     /fragment="True"
                     /other="intron"
                     /description="modified intron A from human cytomegalovirus"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttccgat
      661 tggccttaaa tcatggcctt aaaattctat gatttctctc attacttcct cacatgttgg
      721 aggcattttc tctccctctg cacttaatag cctatcttgc tttaatttct tcccactctt
      781 ggaggctagg tttggtttgg tgggctgatg agggagggag agaccgctcc aagtacttta
      841 gcgggtggga ttgaaggcgg agccctcctg agctatgagt gtcctatgag tggggctgga
      901 actaagaacc aggggcgtgg acagggtgtg tcacagagaa ggggatgtgc ctgcttcttt
      961 ctggcccagg aggaaccggg tcaattcttc agcacctggg tacccataga gcccaccgca
     1021 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     1081 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     1141 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     1201 acagatggct ggcaactaga aggcacagca gatctggatc ctcatcatta tttacagcaa
     1261 gagccgcagc tacaacagcc cttgaggcag ctgcagcagc tagtcataca acaaagcatg
     1321 atggtcacca tgacgatagc gatcaggcct gctataaagc ccagccaaat gtaccaaggc
     1381 catttaatgt actgctcata ttttcccagc tcctgcaggt caataagtga ttcgttgagg
     1441 ttcttggcca cttcgttaag tctatcaatc tctttctgga tgttcaccac gctagcatta
     1501 attcctgaaa tgtcgccgag gtcaacatcg ggtgaggtgt gattcttaaa gtacttatcc
     1561 agttcctcct tgaaagagtc aagttccggt tggagaggat cgtacacggt gttgttcacg
     1621 atcccaataa ctacgtcaca gttcccagat acgaatgtgt tgtcggttgt gattatctgg
     1681 ggctcataaa agttcctctg ggtaacaaac cagtgtgttc cattgctaac aaagacgcct
     1741 tcgcgtggaa agtgtgcctt accgtcgtgg cagatcgccg gagcggtagt aaagttcttc
     1801 tcttgagcgg gaacgtaagt aacatgcagg aacacaacgc catgtggggc ggattggggg
     1861 aagctcatga ggtggtaccc cttcccacaa aagtcgaccc gctttgattg cccgagcacg
     1921 cattcactca tctttgtagc agccagattc gcagatgctc taatctcggc ggctctgata
     1981 agctgctgcg tgacgtacgt ctgcagactc tgcagccttc cagtaataag ccggtcgatc
     2041 tggacttctg cctccacctt atccaatcta ctaaggatat cattcagcac ggaggatata
     2101 gcaccgaact tggatgacag ttgcttgacg agagtattca gggcttgtgc attgtgattg
     2161 actacatctt gcaatttccc gagggcgctg gctgtggaac tcaagctgtc ctggatttta
     2221 ccaatggcgg aattgaactg gttagcgata agtttctggt tttcgtacag gacgttctgg
     2281 gtcaccccga tcccattgaa gcgatatgcc atctgcatgg caaaaggtat ttgcagtgcg
     2341 gctcctgctc cgaacgtcca gccggatgta atagtgccgg ccagcagggc gctggtatat
     2401 tgtgcgatca tctcatcagt gagcagcggt gggagtacgg tcagcccgtt gaatttctgg
     2461 gcacatatca gatcgcgagc agcaatgtct cccaaacagt cgccatactg cttaataaat
     2521 ccagcatcgg cgagggtcac cttattaaag agcaaatcct ctataaagct ccgcttagat
     2581 ggcttagaag gatcaggcag aatttgagag aaattgaacc caccgaagta cttgattggt
     2641 ggcgtcttgt aaatctgctt aacctgcgca aacacctcct gagtgttctt atcctgttcg
     2701 acggcgattc ctgtcagtgc cctcttcagc tgtgtgcaga agctgccgta ctgcaggagc
     2761 aggttggaac attcggtgct atcaccacag atgtacatgg tacagtccac ggaggtttta
     2821 gtcatagaca ctggcaggat ctctgtagtc acagagatgg taaaatttgt ggggatagcg
     2881 atagagttgt tactgtatgc cactgaattt tcagccccca gtgacattgt ataggcgatt
     2941 atggactggg aggccacgct cctagccctt ctgtggctct ttgtctgggt ctgataagag
     3001 gcgcagatac ctgctccaat cggtatatca cattcataag agttgttcac gtactcggct
     3061 ccaatgaggc agccggctct ggtctggaat acattagaac cggtgctgta tacccgccat
     3121 gttggggtca gctggtcggc gtgaatagcc acggggacct cggtacagtt tacaccttga
     3181 tacagcacag cgacttgatt tgaggtattt gtgccgggag taataacact cactcctcca
     3241 aaagaacaag gagtaatgtc cagaatctcc aatgtctggg gatctcgcac cgcatcggtc
     3301 gtgtctgcga tatccctgcc gaactgctgg aatggcagga atttcttatt ggattctgtc
     3361 aacacgcctg tcccggtgag gccattgaag ttgaagttca cgcacttatt tttcaccaga
     3421 ttggtagact ttttaggccc acatactgta gcaggcgcat gaagcagctc aaatgacagc
     3481 accaccaccc gatatggctg gtggccaacg ccgtaagtcg gccgaaatcc atagctttgc
     3541 agcgggctgt aacaattggg cccggcgacc ccgttgcagg gcttgtttcc ggcctggtag
     3601 atctctgttg aaatgtcccg ctcaaaaggt ttcagcttgc tctttcgaaa cagcctatag
     3661 agatagttgt aattgccgct gggtttacta tcgagcttat tggagttcca ggcgatgacg
     3721 cagccggtga aatcatctgg cagtttgtaa ttgtagtcgg ctatgtttcc agtctgccct
     3781 ggggcgattt ggctgacctc gttacctcta ataacgaagc tgtccgcata gacattagta
     3841 aagcacagat cattcagctt cgtaggggag acaccgtagc atttaaaggc gaaaaaggga
     3901 gcgaaattgt agattacgct gtaatcggcc acgcaattgg atatccgttt cctgttccag
     3961 gcgtaaacac tggcaaaggt agttgcgtta aagacctcgt ggaatgggca caaattggta
     4021 atgttgggga agcgaacgat gctctctgtg ggctgaactc tgaagttgct cgtctggtaa
     4081 atgcccttct cgactgtgaa gcttttcagt gtgcatttgg tttcagacaa tgggtccagc
     4141 gcgcaatcaa cagcgtcagt gatggtgccg ttctcgttgt atttgagcag gaaggtccta
     4201 ggctgcagat atcccacata atatgcagca gcacccgcgg tccatcctga ggatgaatcc
     4261 acaggggtca gataagaccg atgcagtgcg agcagagttt ggaagcgcgt gatgttgatg
     4321 ccgataggaa ggtccacgag gggttccagg gcgctaaaac cttgtggcaa atctctctcc
     4381 aggttaatag gggtgtgctt gctgtatatt ttaaaataac cgtcgatgtt cttgaacacg
     4441 aattcgcgca ggttcttaaa gttcccctct ttcccttcga ggtccatcag gaaaggctgg
     4501 cttacgtact caaaagtaca gttatttgca ctggagtaca cgcgaaactc actttccatc
     4561 caagatttat tgttcttctg gtacacgtcg aggaaagggt cgttgcagaa ctggaactcg
     4621 cagactttta tgacgacgtt tgtcgcatta ttcacgatca gcagggattg tgttttacta
     4681 tccagagtcg tgccgaagat ccatccccgg ataatattgc tcttttctgt actggcaaaa
     4741 tacacgccgt cgttaaaagg caaggcggga ttgtcgaatc tcttagtgcc attagtcccg
     4801 gatacgtgta ttgcatgaaa ccaagtcaca tttgagaaga agggcaagaa aaggtcctgc
     4861 gtggagtgca aaacactgct tcggaacacc ttgtcgggat agtagacgcc tcgtgtaaag
     4921 gaattggtgt agctctgtgt tcgcgtgatc aagttgacgc actgactaga caccagggga
     4981 agaagaacca gaaacacgaa cattctagag cggccgcgat atctgatcac acgtgtcgac
     5041 gacggtgact gcagaaaaga cccatggaaa ggaacagtct gttagtctgt cagctattat
     5101 gtctggtggc gcgcgcggca gcaacgagta ctgctcagac tacactgccc tccaccgtta
     5161 actagagttg agcaagcagg gtcaggcaaa gcgtggagag ccggctgagt ctaggtaggc
     5221 tccaagggag cgccggacaa aggcccggtc tcgacctgag ctttaaactt acctagacgg
     5281 cggacgcagt tcaggaggca ccacaggcgg gaggcggcag aacgcgactc aaccggcgtg
     5341 gatggcggcc tcaggtaggg cggcgggcgc gtgaaggaga gatgcgagcc gatggaggct
     5401 ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat
     5461 ctgacggttc actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     5521 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     5581 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     5641 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     5701 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     5761 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     5821 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     5881 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     5941 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     6001 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     6061 tcaacatggc ggtaatgttg gacatgagcc aatataaatg tacatattat gatatggata
     6121 caacgtatgc aatggccaat agccaatctg atgcggtatt ttctccttac gcatctgtgc
     6181 ggtatttcac accgcatatg gtgcaatcat tgggacgtca ggtggcactt ttcggggaaa
     6241 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6301 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6361 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6421 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6481 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6541 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6601 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6661 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6721 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6781 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6841 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6901 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6961 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7021 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7081 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7141 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7201 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7261 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7321 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
