LOCUS       pSS02_NAPstar7         12723 bp    DNA     circular SYN 12-JUN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   WWU Munster - FB Biologie
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12723
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b"
     CDS             join(70..693,694..714,715..1449,1450..1452,1453..2082,
                     2083..2127)
                     /codon_start=1
                     /label=NAPstar7
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /note="This feature has 6 segments:
                        1:   70 ..  693 / #993366 / Rex
                        2:  694 ..  714 / #993366 / Linker
                        3:  715 .. 1449 / #00ff00 / tSapphire
                        4: 1450 .. 1452 / #993366 / Linker
                        5: 1453 .. 2082 / #993366 / Rex
                        6: 2083 .. 2127 / #993366 / Linker"
                     /translation="MVASKVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTA
                     FQVDKDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGF
                     GESFELRGFFSRKAEKVGRPVRGGVIEHVDLLPQRVPGRIEIALLTTPREAAQKAADLL
                     VAAGIKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVY
                     IMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKD
                     PNEKRDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNG
                     HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDF
                     FKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
                     YNTKVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGT
                     DGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFSRKAE
                     KVGRPVRGGVIEHVDLLPQRVPGRIEIALLTTPREAAQKAADLLVAAGIKGILNFAPVV
                     LEVPKEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     CDS             2128..2838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    complement(2840..2864)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(3193..3209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    3217..3233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(3241..3247,3248..3265,3266..3271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 3241 .. 3247 / #ffffff
                        2: 3248 .. 3265 / #ffffff
                        3: 3266 .. 3271 / #ffffff"
     protein_bind    3286..3307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        3498..4175
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /note="color: #ffffff"
     CDS             4243..5268
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #993366"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5308..5482
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    5560..5584
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3"
     CDS             6009..6803
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #993366"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6890..7478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    7664..7804
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      8148..8342
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(8408..9475)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9909..10538)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11838..11862
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3"
     primer_bind     12065..12081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gggtaccggg ccccccctcg aggcgcgcca agctatcaaa caagtttgta caaaaaagca
       61 ggcttcacca tggttgctag caaagttcct gaagcagcca tttccagact gattacttac
      121 ctgagaattc tggaagagct ggaagcccaa ggtgtacacc gcaccgcctc cgagcaactg
      181 ggcgagctgg cccaggtcac cgccttccag gttgataagg acctgtccta cttcggctcc
      241 tacggcactg acggcgttgg ctacactgta ccggtcctca agagagaact gcgccatatc
      301 ctcggtctga accgcaaatg gggcctgtgc atcgtgggga tgggccgcct gggatccgct
      361 cttgctgatt ggcctggttt tggcgagagc ttcgagctgc gcggtttctt ctctagaaag
      421 gctgagaagg ttggtcggcc ggttcgcggt ggtgttatcg aacacgtcga tctgctgccc
      481 cagcgcgtac ctggtcgcat cgaaatcgct ctgctgacga ctccacgcga ggcagcacag
      541 aaggctgccg acctgctggt tgccgctggc atcaagggca tcctgaactt cgctccagtt
      601 gtactggagg ttcccaagga agtggctgtt gagaacgttg acatcctggc cggtctgacg
      661 cgtctgagct ttgccattct gaaccccacg tggagcgcag caggtgggca tggtttcact
      721 gctcacaacg tgtatatcat ggctgacaaa cagaagaacg gtatcaaggc caacttcaag
      781 atccgccaca acatcgaaga cggcggcgtt cagctggctg atcactacca gcagaacacc
      841 ccaatcggcg acggccctgt tctgctgcct gacaaccact acttaagcat ccagtctaaa
      901 ctgtccaaag acccaaacga gaagcgcgac cacatggttc tgctggaatt tgttaccgcc
      961 gctggcatca cccacggcat ggacgaactg tacaagggcg gcaccggggg atctatggtt
     1021 tccaaaggcg aggaactgtt cactggtgtt gttcctatcc tggttgaact ggacggcgac
     1081 gttaacggcc acaaattcag cgttagcggg gaaggcgaag gagacgctac ctacggcaag
     1141 ctgactctga agttcatctg caccactggc aagctgcctg ttccttggcc taccctggtt
     1201 actaccttct cctatggtgt tatggttttc gccagatatc ctgaccacat gaagcagcac
     1261 gacttcttca agagcgccat gcctgagggc tacgttcagg aacgcaccat ctttttcaag
     1321 gacgacggca actataagac acgcgctgag gttaagttcg agggcgacac tctggttaac
     1381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg ccataagctt
     1441 gaatataaca ccaaggtacc tgaagccgcc atcagccgcc tcatcactta cctccgcatc
     1501 ctggaggagc tcgaagccca aggcgttcac cgcactgcaa gcgagcagct gggcgaactg
     1561 gcacaggtta cagcattcca ggttgacgaa gatctgtcct atttcggttc ctacggcacc
     1621 gacggtgttg gctacacagt ccctgtcctg aagcgcgagc tgcgccacat cctgggcctg
     1681 aaccgcaagt ggggcttatg catcgttggc atgggccgcc tcggtagtgc actggcagac
     1741 tggccgggct ttggtgaatc tttcgaactg cgcggcttct tctcaaggaa ggctgagaag
     1801 gttgggcgcc ctgttcgcgg tggcgttatc gagcatgtcg acctcctgcc acagcgcgtc
     1861 ccgggtcgca tcgagatcgc cctgctgacc actcctcgcg aagctgcaca gaaggcagcc
     1921 gacttactag tcgccgctgg tatcaaggga attctgaact tcgcccctgt tgttctggag
     1981 gttcctaagg aagttgctgt tgaaaacgtt gatttcctgg ccggcctgac ccgcctgagc
     2041 ttcgcaatcc tgaatcctaa gtggcgcgaa gaaatgatgg gttccggaac tgggggcaac
     2101 gcctctgacg gtggtgggtc tggtggtatg gtgagcaagg gcgaggagga taacatggcc
     2161 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2281 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2581 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2641 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2701 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2761 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2821 gacgaactgt acaagtgagt cccagctttc ttgtacaaag tggttcgata attccttaat
     2881 taactagttc tagagcggcc gcccaccgcg gtggagctcg aatttccccg atcgttcaaa
     2941 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     3001 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     3061 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3121 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactgaa
     3181 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3241 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3301 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
     3421 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     3481 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     3541 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     3601 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     3661 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     3721 acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctcgtctac
     3781 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     3841 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     3901 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     3961 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     4021 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     4081 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagacct tcctctatat
     4141 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
     4201 tatctctctc gagctttcgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
     4261 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     4321 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     4381 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     4441 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     4501 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     4561 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     4621 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     4681 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     4741 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     4801 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     4861 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     4921 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     4981 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     5041 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     5101 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     5161 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     5221 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     5281 ccggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     5341 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     5401 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     5461 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
     5521 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5581 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5641 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     5701 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     5761 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     5821 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     5881 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     5941 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     6001 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     6061 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     6121 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     6181 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     6241 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     6301 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     6361 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     6421 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     6481 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     6541 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     6601 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     6661 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     6721 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     6781 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     6841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7021 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7621 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7681 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7741 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7801 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7861 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7921 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     7981 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8041 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     8101 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     8161 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     8221 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     8281 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     8341 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     8401 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     8461 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     8521 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     8581 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     8641 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     8701 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     8761 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     8821 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     8881 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     8941 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9001 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9061 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     9121 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     9181 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     9241 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     9301 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     9361 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     9421 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac
     9481 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
     9541 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
     9601 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
     9661 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
     9721 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
     9781 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
     9841 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
     9901 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
     9961 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    10021 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    10081 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    10141 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    10201 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    10261 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    10321 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    10381 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    10441 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    10501 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    10561 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    10621 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    10681 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    10741 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    10801 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    10861 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    10921 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    10981 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    11041 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    11101 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    11161 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    11221 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    11281 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    11341 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    11401 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    11461 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    11521 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    11581 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    11641 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    11701 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    11761 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgtt attctaataa
    11821 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
    11881 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg
    11941 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    12001 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    12061 cgttgtaaaa cgacggccag tgccaagctt cgacgagtca gtaataaacg gcgtcaaagt
    12121 ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca
    12181 acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg
    12241 tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct
    12301 cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt
    12361 cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag
    12421 gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa
    12481 tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg
    12541 tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg
    12601 ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc
    12661 tggtgttagt ttctagtttg tgcgatcgaa tttgtagatt aatctgagtt tttctgatta
    12721 aca
//
