LOCUS       Plasmid_241239-        6743 bp DNA     circular SYN 21-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   VB250109-1642mec
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 22, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Sequence Label: JN196 Fos-FLpoERT2-WPRE
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
     promoter        158..868
                     /label={Fos}
                     /note="/application_notes="
                     /note="/official_designation={Fos}"
                     /note="/uuid=4587c32312074bab936ca83e52217990"
     misc_feature    893..898
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             899..3121
                     /label={Flpo-ERT2}
                     /note="/application_notes="
                     /note="/official_designation={Flpo-ERT2}"
                     /note="/uuid=f8d38ba2f1284eeeaade7ed86c0fe0cd"
     misc_feature    3152..3749
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     misc_feature    3780..3987
                     /label=BGH pA
                     /label=nonstandard type:  Regulatory_Elem
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     misc_feature    complement(3995..4124)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             5052..5912
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
     rep_origin      6083..6671
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgagc tttcctttag gaacagaggc
      181 ttcgagcctt taaggctgcg tacttgcttc tcctaatacc agagactcaa aaaaaaaaaa
      241 aaagttccag attgctggac aatgacccgg gtctcatccc ttgaccctgg gaaccgggtc
      301 cacattgaat caggtgcgaa tgttcgctcg ccttctctgc ctttcccgcc tcccctcccc
      361 cggccgcggc cccggttccc cccctgcgct gcaccctcag agttggctgc agccggcgag
      421 ctgttcccgt caatccctcc ctcctttaca caggatgtcc atattaggac atctgcgtca
      481 gcaggtttcc acggccggtc cctgttgttc tggggggggg accatctccg aaatcctaca
      541 cgcggaaggt ctaggagacc ccctaagatc ccaaatgtga acactcatag gtgaaagatg
      601 tatgccaaga cgggggttga aagcctgggg cgtagagttg acgacagagc gcccgcagag
      661 ggccttgggg cgcgcttccc cccccttcca gttccgccca gtgacgtagg aagtccatcc
      721 attcacagcg cttctataaa ggcgccagct gaggcgccta ctactccaac cgcgactgca
      781 gcgagcaact gagaagactg gatagagccg gcggttccgc gaacgagcag tgaccgcgct
      841 cccacccagc tctgctctgc agctcccaca agtttgtaca aaaaagcagg ctgccaccat
      901 gagccagttc gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga
      961 gagattcgag aggcccagcg gcgagaagat cgccagctgt gccgccgagc tgacctacct
     1021 gtgctggatg atcacccaca acggcaccgc catcaagagg gccaccttca tgagctacaa
     1081 caccatcatc agcaacagcc tgagcttcga catcgtgaac aagagcctgc agttcaagta
     1141 caagacccag aaggccacca tcctggaggc cagcctgaag aagctgatcc ccgcctggga
     1201 gttcaccatc atcccttaca acggccagaa gcaccagagc gacatcaccg acatcgtgtc
     1261 cagcctgcag ctgcagttcg agagcagcga ggaggccgac aagggcaaca gccacagcaa
     1321 gaagatgctg aaggccctgc tgtccgaggg cgagagcatc tgggagatca ccgagaagat
     1381 cctgaacagc ttcgagtaca ccagcaggtt caccaagacc aagaccctgt accagttcct
     1441 gttcctggcc acattcatca actgcggcag gttcagcgac atcaagaacg tggaccccaa
     1501 gagcttcaag ctggtgcaga acaagtacct gggcgtgatc attcagtgcc tggtgaccga
     1561 gaccaagaca agcgtgtcca ggcacatcta ctttttcagc gccagaggca ggatcgaccc
     1621 cctggtgtac ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga gagtgaacag
     1681 gaccggcaac agcagcagca acaagcagga gtaccagctg ctgaaggaca acctggtgcg
     1741 cagctacaac aaggccctga agaagaacgc cccctacccc atcttcgcta tcaagaacgg
     1801 ccctaagagc cacatcggca ggcacctgat gaccagcttt ctgagcatga agggcctgac
     1861 cgagctgaca aacgtggtgg gcaactggag cgacaagagg gcctccgccg tggccaggac
     1921 cacctacacc caccagatca ccgccatccc cgaccactac ttcgccctgg tgtccaggta
     1981 ctacgcctac gaccccatca gcaaggagat gatcgccctg aaggacgaga ccaaccccat
     2041 cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc gagggcagca tcagataccc
     2101 cgcctggaac ggcatcatca gccaggaggt gctggactac ctgagcagct acatcaacag
     2161 gcggatcctc gagccatctg ctggagacat gagagctgcc aacctttggc caagcccgct
     2221 catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt
     2281 cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc
     2341 cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca
     2401 catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt
     2461 ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat
     2521 ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa
     2581 atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg
     2641 catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc
     2701 tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca
     2761 ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac
     2821 cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca
     2881 catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct
     2941 ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca ctagccgtgg
     3001 aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc
     3061 gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacagcttg
     3121 aacccagctt tcttgtacaa agtgggaatt ccgataatca acctctggat tacaaaattt
     3181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3541 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3721 tctccctttg ggccgcctcc ccgcatcggg aattcctaga gctcgctgat cagcctcgac
     3781 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3841 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3901 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3961 ggaagagaat agcaggcatg ctggggaggg ccgcaggaac ccctagtgat ggagttggcc
     4021 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4081 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4141 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4201 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4261 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     4321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4501 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     4561 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     4621 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4681 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4741 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4801 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4861 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4921 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5761 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6001 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6721 ttgctcacat gtcctgcagg cag
//
