LOCUS       Exported                5769 bp ds-DNA     circular SYN 12-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    TetOHyperTRIBE_Adar_only_mcherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..285
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        318..356
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    507..509
                     /note="Mex3a"
     misc_feature    512
                     /note="Mex3a"
     misc_feature    546..1694
                     /note="deaminase"
     misc_feature    927..1046
                     /note="5' RNA binding loop"
     misc_feature    1053..1055
                     /note="EDIT me to Glutamine!"
     misc_feature    1713..1754
                     /note="V5 epitope"
     CDS             1776..1829
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1836..2543
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     intron          2565..3137
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      3334..3389
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     rep_origin      complement(3951..4539)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4710..5570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5571..5675)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
      481 gtcagctgac gcgtgctagc gcggccatgt ccggccgcat cgataagctt ggaggtggag
      541 gtagtacctt ccacgaccag atagctatgc tgagccacag gtgcttcaat gctctgacca
      601 acagtttcca gccctccctg ctcggccgca agatcctggc tgccattatt atgaaaagag
      661 atccagagga catgggtgtt gtcgtgagtt tggggacagg caatcgctgt gtgaaagggg
      721 actctctcag cctgaaggga gagacggtca atgactgcca tgccgaaatc atctcccgga
      781 ggggcttcat caggtttctc tacagtgaac tgatgaagta caaccaccac actgccaaga
      841 acagcatatt tgagcttgcc aggggaggag agaagctgca gataaagaag acggtttctt
      901 ttcatctcta catcagcacg gcgccatgtg gagatggggc cctctttgac aaatcctgca
      961 gtgaccgtgc cgtggaaagc acagagtccc gccattaccc tgtctttgaa aatcccaagc
     1021 aaggcaagct tcgcaccaag gtggagaatg ggcaaggcac aattcctgtg gagtccagtg
     1081 atattgtacc cacgtgggat ggcatccgcc ttggggaaag actccgtacc atgtcctgta
     1141 gtgacaaaat cctacgctgg aatgtgctgg gcctgcaagg ggcgttgttg acgcacttcc
     1201 tacagcctgt gtacctgaaa tctgtaacct taggttacct tttcagccaa gggcatctga
     1261 cccgtgctat ttgctgccgc gtgaccagag atgggaaagc atttgaggat ggactacgct
     1321 atccctttat tgtcaaccac cccaaggtcg gccgagtcag tgtttatgat tccaaaagac
     1381 agtccggaaa gaccaaggaa acaagcgtca actggtgcat ggctgatggc tatgacctag
     1441 agatcctgga tggcaccaga ggcactgtgg atggaccagg gaaagagttg tctcgggtgt
     1501 ccaagaagaa tattttcctt cagtttaaga agctctgctc cttccgagcc cgcagagatt
     1561 tactgcagct ctcttatggt gaagccaaga aagctgcccg tgactatgac ttagccaaga
     1621 actacttcaa gaaaagcctg cgagacatgg gctatgggaa ttggatcagc aaaccccagg
     1681 aggaaaagaa ctttacggat ccccagccat ggggtaagcc tatccctaac cctctcctcg
     1741 gtctcgattc tacgaagctt gtcgacggca gtggagaggg cagaggaagt ctgctaacat
     1801 gcggtgacgt cgaggagaat cctggcccat ctagagtgag caagggcgag gaggataaca
     1861 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1921 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1981 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2041 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2101 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2161 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2221 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2281 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2341 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2401 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2461 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2521 gcatggacga gctgtacaag taatctagag ctgagaactt cagggtgagt ttggggaccc
     2581 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt
     2641 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
     2701 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
     2761 tttctgtaac ttttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact
     2821 ttcgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag
     2881 ggtaattata ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag
     2941 gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca
     3001 actacatcct ggtaatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg
     3061 agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct
     3121 tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg
     3181 gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     3241 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     3301 tcatgaagcc ccttgagcat ctgacttctg ggtaataaag gaaatttatt ttcattgcaa
     3361 tagtgtgtgg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     3421 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc
     3481 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     3541 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     3601 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     3661 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaac tcgactgcat
     3721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4141 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4381 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     4861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5701 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     5761 ccctttcgt
//
