LOCUS       Exported                7323 bp ds-DNA     circular SYN 12-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    TetO-Mex3a_Adar_V5_2a_mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..285
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        318..356
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    507..2066
                     /note="Mex3a"
     misc_feature    930..1085
                     /note="KH domain"
     misc_feature    1179..1361
                     /note="KH domain"
     misc_feature    1911..2030
                     /note="RING Finger"
     misc_feature    2100..3248
                     /note="deaminase"
     misc_feature    2481..2600
                     /note="5' RNA binding loop"
     misc_feature    2607..2609
                     /note="EDIT me to Glutamine!"
     misc_signal     2608..2609
                     /note="EDIT ME to Glutamine!"
     misc_feature    3267..3308
                     /note="V5 epitope"
     CDS             3330..3383
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3390..4097
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     intron          4119..4691
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      4888..4943
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     rep_origin      complement(5505..6093)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6264..7124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7125..7229)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
      481 gtcagctgac gcgtgctagc gcggccatgc ctagtctagt ggtatctgga ataatggaaa
      541 gaaatggggg ctttggagaa ctaggatgtt tcgggggaag cgctaaagac agggggcttc
      601 tggaagacga gcgcgccctc cagctggcac tggaccagct ctgccttctg gggctgggcg
      661 agccgcctgc acccaccgcc ggagaagatg gtgggggagg gggcggcgga gcaccagccc
      721 agcctgcagc accacctcag ccagctcccc ctcctcctcc agcagccgct cccgctgctc
      781 caagcgcagc ccctgccgct caaaccccac aacctcccac agcccctaag ggcgcaaacg
      841 atgccaagct gtgtgccctg tataaagagg cagaattgag gcttaaagga agctcaaaca
      901 ccacggagtg cgttcccgtg cccacctccg agcacgtggc cgagatcgtg ggcaggcaag
      961 gctgcaagat taaggctctg agggccaaaa ccaacaccta catcaagaca cctgtgcgag
     1021 gtgaggagcc agtgttcatg gtgactggga ggcgggaaga cgtggccaca gcccggcggg
     1081 agatcatctc tgcagctgag cacttctcca tgataagagc ctcacgcaac aagtctggcg
     1141 ccgcctttgg cgtggcccct gctctgccag gccaagtgac catccgtgta cgagtaccct
     1201 accgcgtggt gggactggtg gtgggcccca agggagcgac catcaaacgc atccagcagc
     1261 agaccaacac gtacatcatc actcccagcc gggaccgcga ccccgtgttt gagataaccg
     1321 gcgcaccggg caacgtggag cgtgcgcggg aggagatcga gacgcacatc gctgtgcgca
     1381 ccggcaagat tcttgaatac aacagcgacg gcgacttcct ggctggcagc cctgacgctg
     1441 ccttggacag ccgctactca gacgcctggc gggtgcatgc tccaggctgt aagccgctct
     1501 ccaccttcag gcagaacagc ctgggctgca taggcgaatg cggcgtggac tcggcctttg
     1561 aggccccgcg cctaagcgag cagggcgggg actttggcta cagcggatat ctgtttccgg
     1621 gctacggtgt gggcaagcag gacgtgtact atggcgtggc agagacaagc cccccgctgt
     1681 gggcgggtca ggagaatgcc acgcccacct cggtgctctt ctcctctgcc tcctcctcct
     1741 cctcttcagc caaggcacgc gcgggccccc caggcgcgca tcgctctcct gccacctctg
     1801 cggggccaga gcagctgacc gggctccctc ggaggccacc cggagagcca ctgcagggtt
     1861 tctctaaact ggggacaggc gggctgcgga gccccggcag cgggcgggac tgcatggtgt
     1921 gctttgagag tgaagtgaca gcagccctgg tgccctgtgg acacaacctt ttctgcatgg
     1981 agtgtgcagt acgcatctgc gagaggacgg acccagagtg tcctgtctgc cacatcaccg
     2041 ccacacaagc catccgaata ttttccggcc gcatcgataa gcttggaggt ggaggtagta
     2101 ccttccacga ccagatagct atgctgagcc acaggtgctt caatgctctg accaacagtt
     2161 tccagccctc cctgctcggc cgcaagatcc tggctgccat tattatgaaa agagatccag
     2221 aggacatggg tgttgtcgtg agtttgggga caggcaatcg ctgtgtgaaa ggggactctc
     2281 tcagcctgaa gggagagacg gtcaatgact gccatgccga aatcatctcc cggaggggct
     2341 tcatcaggtt tctctacagt gaactgatga agtacaacca ccacactgcc aagaacagca
     2401 tatttgagct tgccagggga ggagagaagc tgcagataaa gaagacggtt tcttttcatc
     2461 tctacatcag cacggcgcca tgtggagatg gggccctctt tgacaaatcc tgcagtgacc
     2521 gtgccgtgga aagcacagag tcccgccatt accctgtctt tgaaaatccc aagcaaggca
     2581 agcttcgcac caaggtggag aatgggcaag gcacaattcc tgtggagtcc agtgatattg
     2641 tacccacgtg ggatggcatc cgccttgggg aaagactccg taccatgtcc tgtagtgaca
     2701 aaatcctacg ctggaatgtg ctgggcctgc aaggggcgtt gttgacgcac ttcctacagc
     2761 ctgtgtacct gaaatctgta accttaggtt accttttcag ccaagggcat ctgacccgtg
     2821 ctatttgctg ccgcgtgacc agagatggga aagcatttga ggatggacta cgctatccct
     2881 ttattgtcaa ccaccccaag gtcggccgag tcagtgttta tgattccaaa agacagtccg
     2941 gaaagaccaa ggaaacaagc gtcaactggt gcatggctga tggctatgac ctagagatcc
     3001 tggatggcac cagaggcact gtggatggac cagggaaaga gttgtctcgg gtgtccaaga
     3061 agaatatttt ccttcagttt aagaagctct gctccttccg agcccgcaga gatttactgc
     3121 agctctctta tggtgaagcc aagaaagctg cccgtgacta tgacttagcc aagaactact
     3181 tcaagaaaag cctgcgagac atgggctatg ggaattggat cagcaaaccc caggaggaaa
     3241 agaactttac ggatccccag ccatggggta agcctatccc taaccctctc ctcggtctcg
     3301 attctacgaa gcttgtcgac ggcagtggag agggcagagg aagtctgcta acatgcggtg
     3361 acgtcgagga gaatcctggc ccatctagag tgagcaaggg cgaggaggat aacatggcca
     3421 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     3481 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3541 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     3601 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3661 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3721 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3781 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     3841 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3901 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3961 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     4021 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     4081 acgagctgta caagtaatct agagctgaga acttcagggt gagtttgggg acccttgatt
     4141 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     4201 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     4261 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     4321 taactttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cactttcgtt
     4381 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtaat
     4441 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     4501 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     4561 tcctggtaat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     4621 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     4681 ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     4741 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4801 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4861 agccccttga gcatctgact tctgggtaat aaaggaaatt tattttcatt gcaatagtgt
     4921 gtgggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4981 atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa
     5041 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     5101 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     5161 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     5221 tcctgactac tcccagtcat agctgtccct cttctcttat gaactcgact gcattaatga
     5281 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     5701 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5941 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6241 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6301 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6361 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
     6421 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6481 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6541 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6601 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6661 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6721 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6781 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6841 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6901 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6961 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7021 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7081 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7261 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     7321 cgt
//
