LOCUS       pMPS55_pDestTol2       11234 bp    DNA     circular SYN 07-JUN-2025
DEFINITION  pMPS55.
ACCESSION   pMPS55
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..11234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(174..1031)
                     /label=ampR
     rep_origin      1162..1487
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.1"
                     /note="/match_length=72.1"
                     /note="/other=rep_origin"
     LTR             complement(1773..1789)
                     /label=Tol2 3' TIR
     misc_structure  complement(1962..2308)
                     /label=Tol2 exon4 136-482
     primer_bind     2320..2341
                     /label=M13R
     primer_bind     2320..2341
                     /label=M13R
                     /label=M13R(1)
     protein_bind    2356..2376
                     /label=attB4
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    2363..2376
                     /label=attL4
     primer_bind     2393..2432
                     /label=Ubi_in fusion For
     misc_feature    2408..5890
                     /label=Ubi
     primer_bind     complement(5861..5905)
                     /label=Ubi_in fusion Rev
     protein_bind    5917..5940
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5917..5937
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     complement(5924..5948)
                     /label=oAMS1186
     primer_bind     5934..5976
                     /label=Fragment.FOR
     primer_bind     5934..5951
                     /label=Fragment.FOR
     CDS             5967..6395
                     /codon_start=1
                     /label=N41_CNBD
                     /translation="MFYQEVRRGDFMRNWQLVAAVPLFQKLGPAVLVEIVRALRARTVP
                     AGAVICRIGESGDRMFFVVEGSVSVATPNPVELGPGAFFGEMALISGEPQSATVSAATT
                     VSLLSLHSADFQMLCSSSPEIAEIIRKTALERRGAAANA"
     primer_bind     complement(6220..6238)
                     /label=Primer 2
     primer_bind     6239..6261
                     /label=Primer 1
     primer_bind     6239..6261
                     /label=Primer 3
     CDS             6249..6251
                     /label=R307
     CDS             6402..7118
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFG
                     FVTAAAITHGMDELYK"
     primer_bind     complement(7093..7118)
                     /label=Fragment.REV
     primer_bind     complement(7120..7134)
                     /label=Ubi_in fusion Rev
     promoter        7134..7151
                     /label=T7 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.7"
                     /note="/other=promoter"
     primer_bind     7155..7176
                     /label=gibson PolyA F
     polyA_signal    7156..7290
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     7297..7320
                     /label=oAMS1187
     primer_bind     complement(7297..7313)
                     /label=Fragment.REV
     protein_bind    7307..7327
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7330..7462
                     /label=SV40 late polyA (from pCS2+)
                     /label=SV40 late polyA (from pCS2+)(1)
     primer_bind     7330..7350
                     /label=gibson PolyA F
     protein_bind    complement(7527..7547)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_recomb     7534..7547
                     /label=attR3
     misc_feature    7534..7541
                     /label=att3_shared
     CDS             7551..7571
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=12.1"
                     /note="/other=CDS"
                     /translation="IHWPSFY"
     primer_bind     complement(7556..7571)
                     /label=M13F
     polyA_signal    7587..7719
                     /label=SV40 late polyA (from pCS2+)
                     /label=SV40 late polyA (from pCS2+)(1)
     primer_bind     7587..7607
                     /label=gibson PolyA F
     primer_bind     7817..7859
                     /label=Gibson cmlc2 F
     promoter        7843..8741
                     /label=cmlc2 promoter
     primer_bind     complement(8720..8761)
                     /label=gibson cmlc2 R
     primer_bind     8722..8761
                     /label=gibson mCherry F
     misc_feature    8742..9462
                     /label=mcherry
     primer_bind     complement(9116..9138)
                     /label=mCherry R
     primer_bind     9119..9139
                     /label=mCherry F
     primer_bind     9447..9483
                     /label=gibson PolyA F
     polyA_signal    9463..9595
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.5"
                     /note="/other=polyA_signal"
     misc_structure  complement(9602..9936)
                     /label=Tol2 exon1 bp1-335 of 461
     LTR             complement(10100..10118)
                     /label=Tol2 5' TIR
     misc_structure  complement(10119..10155)
                     /label=medaka tyrosinase ex5 frag
     misc_feature    10152..10192
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.0"
                     /note="/other=misc_feature"
     primer_bind     10174..10181
                     /label=Primer 1
     primer_bind     10174..10181
                     /label=Primer 3
     primer_bind     complement(10182..10193)
                     /label=Primer 1
     primer_bind     complement(10182..10193)
                     /label=Primer 3
     promoter        complement(10204..10223)
                     /label=T3
     promoter        10205..10223
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     complement(10239..10260)
                     /label=M13R
                     /label=M13R(1)
     primer_bind     complement(10239..10260)
                     /label=M13R
     protein_bind    10266..10288
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        10292..10322
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    10337..10358
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(10371..10463)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      10646..11234
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaaggct ggctaagaac
     1501 tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc acaatgacgg
     1561 ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc tgtccaacaa
     1621 tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa caccagaaat
     1681 gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca ctctgttcca
     1741 cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac tcaaaaattt
     1801 tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc
     1861 tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta
     1921 ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta agcttttaat
     1981 ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa ctgtttgtat
     2041 ttaattatca aactataaga cagacaatct aatgccagta cacgctactc aaagttgtaa
     2101 aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc aagcctagct
     2161 cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg
     2221 ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac
     2281 tccctggtgt ctgaaacaca ggccagatgg gccctcgagc aggaaacagc tatgaccatg
     2341 attacgccaa gctatcaact ttgtatagaa aagttgctgg atcccatcga ttcgaattca
     2401 aggcctcacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2461 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2521 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2581 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2641 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2701 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2761 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2821 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2881 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2941 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     3001 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     3061 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     3121 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     3181 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3241 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3301 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3361 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3421 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3481 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3541 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3601 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3661 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3721 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3781 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3841 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3901 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3961 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     4021 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     4081 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     4141 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     4201 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4261 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4321 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4381 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4441 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4501 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4561 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4621 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4681 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4741 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4801 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4861 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4921 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4981 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     5041 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     5101 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     5161 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     5221 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5281 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5341 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5401 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5461 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5521 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5581 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5641 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5701 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5761 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5821 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5881 ttgtttacag tcgagcctct agaaccggtg gtaccgcaag tttgtacaaa aaagcaggct
     5941 ccgaattcgc ccttggatcc gccaccatgt tctaccagga ggtgaggagg ggcgacttca
     6001 tgaggaactg gcagctggtg gccgccgtgc ctctgttcca gaagctgggc cctgccgtgc
     6061 tggtggagat cgtgagggcc ctgagggcca ggaccgtgcc tgccggcgcc gtgatctgca
     6121 ggatcggcga gtctggcgac aggatgttct tcgtggtgga gggcagcgtg agcgtggcca
     6181 cccctaaccc tgtggagctg ggccctggcg ccttcttcgg cgagatggcc ctgatcagcg
     6241 gcgagcctca gagcgccacc gtgagcgccg ccaccaccgt gagcctgctg agcctgcaca
     6301 gcgccgactt ccagatgctg tgcagcagca gccctgagat cgccgagatc atcaggaaga
     6361 ccgccctgga gaggagaggc gctgccgcta acgccgaatt cgtgagcaag ggcgccgagc
     6421 tgttcaccgg catcgtgccc atcctgatcg agctgaatgg cgatgtgaat ggccacaagt
     6481 tcagcgtgag cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     6541 tctgcaccac cggcaagctg cctgtgccct ggcccaccct ggtgaccacc ctgagctacg
     6601 gcgtgcagtg cttctcacgc taccccgatc acatgaagca gcacgacttc ttcaagagcg
     6661 ccatgcctga gggctacatc caggagcgca ccatcttctt cgaggatgac ggcaactaca
     6721 agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc gagctgaccg
     6781 gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac aactacaacg
     6841 cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg aacttcaaga
     6901 tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag cagaataccc
     6961 ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc cagagcgccc
     7021 tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc gtgaccgccg
     7081 ccgccatcac ccacggcatg gatgagctgt acaagtgact cgagcctcta gaactatagt
     7141 gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     7201 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7261 ggaaccatta taagctgcaa taaacaagtt aagggcgaat tcgacccagc tttcttgtac
     7321 aaagtggggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7381 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7441 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7501 cagggggagg tgtgggaggt tttttccaac tttattatac atagttgata attcactggc
     7561 cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg
     7621 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7681 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     7741 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     7801 acaaatgtgg tatggctgat tatgatcctc tagatcagat ctaaagctta aatcagttgt
     7861 gttaaataag agacattcaa aataaatgta aatgagctct ccaaatcagc agacttaaca
     7921 ttctttaaaa tgattgattc aatagtgata aaaatcaggc atagccagtt gtaactttag
     7981 ataaattaca gaaaatgtca aatacagaga accgattctt ttttatgata catccaagca
     8041 cacatttaac acaatccagg caaaccccga atttcacagt cacaagcact gtttgtacaa
     8101 gagctttgcc taaggacaca cagtctctat aagtccaggt cgttggtttc actcttattt
     8161 taaacatgtg acatttttcc tgccatcctg tcttaggctg ctgtttgctt cattccatgt
     8221 cacattaaat tcctcagtag caccttttac acacacagcc aatcttttcc agaaaattca
     8281 attgctttga agagataatg tgtgaacaaa tccatttaga aaaggaaaat taagaatttg
     8341 taaaatcatc tgtaaattgt tggcattctt ctgtatatga acatcacatc atttacaggt
     8401 aaaggtctgg tcattaatta tatgacaatt tactggtatt attttgtgaa aggggctatt
     8461 ttcaatgcgt tcatccatcc ttttcatccc tcaaatctct cattcacgtc cccctcccca
     8521 tctgcacact ttatctcatt ttccaccctg ctggaatctg agcacttgtg cagttatcag
     8581 ggctcctgta tttaggaggc tctgggtgtc catgtagggg acgaacagaa acactgcaga
     8641 cctttataga agaacaattg ataagagtcc tcatacataa agactccatt agtaagccag
     8701 tgacccagga gcccagacca acagcaaagc agacagtgac cggctccacc atggtgagca
     8761 agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     8821 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     8881 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     8941 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     9001 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     9061 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     9121 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     9181 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     9241 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     9301 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagc
     9361 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     9421 gccgccactc caccggcggc atggacgagc tgtacaagct gagatccaga catgataaga
     9481 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     9541 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagagatc
     9601 tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg
     9661 cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt
     9721 ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt
     9781 ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg
     9841 cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata
     9901 gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
     9961 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
    10021 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
    10081 gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag
    10141 caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
    10201 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
    10261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10321 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10381 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11221 aaaaaggatc tcaa
//
