LOCUS       Exported               10510 bp ds-DNA     circular SYN 13-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="from myobacteriumphage bxb1 (Thomson et al 2012)"
     protein_bind    1802..1835
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1875..2268
                     /label=TRE-3G
     protein_bind    1881..1899
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1917..1935
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1953..1971
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1989..2007
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2025..2043
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2061..2079
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2097..2115
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     regulatory      2263..2272
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    2269..3297
                     /label=ETV2
     misc_feature    3296..3884
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3941..5618
                     /label=pCAG
     misc_feature    5677..6420
                     /label=Tet-On(R) 3G
     CDS             6421..6474
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6475..7182
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             7207..7227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7231..7251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7255..7275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7346..7570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7599..7631
                     /label=lox251
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     rep_origin      7868..8323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8605..8709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8710..9570
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9741..10329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggtctccg tcgtcaggat catccggatc
     1801 cataacttcg tatagcatac attatacgaa gttatcatga tattcggcaa gcaggcatcg
     1861 actagttaat taaggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1921 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1981 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     2041 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     2101 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
     2161 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
     2221 aactttccgt accacttcct accctcgtaa acttaagaat tcgccaccat ggacctgtgg
     2281 aactgggatg aggcatcccc acaggaagtg cctccaggga acaagctggc agggcttgaa
     2341 ggagccaaat taggcttctg tttccctgat ctggcactcc aaggggacac gccgacagcg
     2401 acagcagaga catgctggaa aggtacaagc tcatccctgg caagcttccc acagctggac
     2461 tggggctccg cgttactgca cccagaagtt ccatgggggg cggagcccga ctctcaggct
     2521 cttccgtggt ccggggactg gacagacatg gcgtgcacag cctgggactc ttggagcggc
     2581 gcctcgcaga ccctgggccc cgcccctctc ggcccgggcc ccatccccgc cgccggctcc
     2641 gaaggcgccg cgggccagaa ctgcgtcccc gtggcgggag aggccacctc gtggtcgcgc
     2701 gcccaggccg ccgggagcaa caccagctgg gactgttctg tggggcccga cggcgatacc
     2761 tactggggca gtggcctggg cggggagccg cgcacggact gtaccatttc gtggggcggg
     2821 cccgcgggcc cggactgtac cacctcctgg aacccggggc tgcatgcggg tggcaccacc
     2881 tctttgaagc ggtaccagag ctcagctctc accgtttgct ccgaaccgag cccgcagtcg
     2941 gaccgtgcca gtttggctcg atgccccaaa actaaccacc gaggtcccat tcagctgtgg
     3001 cagttcctcc tggagctgct ccacgacggg gcgcgtagca gctgcatccg ttggactggc
     3061 aacagccgcg agttccagct gtgcgacccc aaagaggtgg ctcggctgtg gggcgagcgc
     3121 aagagaaagc cgggcatgaa ttacgagaag ctgagccggg gccttcgcta ctactatcgc
     3181 cgcgacatcg tgcgcaagag cggggggcga aagtacacgt accgcttcgg gggccgcgtg
     3241 cccagcctag cctatccgga ctgtgcggga ggcggacggg gagcagagac acaataatca
     3301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3661 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     3721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3781 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3841 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgccctttg ggccgcctcc
     3901 ccgcgctagt gctggggatg cggtgggctc tatgggtctc gacattgatt attgactagt
     3961 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4021 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4081 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4141 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4201 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4261 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4321 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     4381 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     4441 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     4501 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     4561 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc
     4621 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     4681 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     4741 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     4801 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     4861 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     4921 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     4981 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     5041 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     5101 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     5161 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     5221 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     5281 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     5341 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     5401 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     5461 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     5521 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     5581 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     5641 ttgtgctgtc tcatcatttt ggcaaagaat gtcgacatga gccggctgga caagagcaaa
     5701 gtgatcaaca gcgccctgga actgctgaac ggcgtgggca tcgagggcct gaccacccgg
     5761 aagctggccc agaaactggg cgtggaacag cccaccctgt actggcacgt gaagaacaag
     5821 cgggccctgc tggacgccct gcccatcgag atgctggacc ggcaccacac ccacagctgc
     5881 cctctggaag gcgagagctg gcaggacttc ctgcggaaca acgccaagag ctacagatgc
     5941 gccctgctga gccaccggga cggcgccaaa gtgcacctgg gcaccagacc caccgagaag
     6001 cagtacgaga cactggaaaa ccagctggcc ttcctgtgcc agcagggctt cagcctggaa
     6061 aacgccctgt acgccctgag cgccgtgggc cacttcaccc tgggctgcgt gctggaagaa
     6121 caggaacacc aggtcgccaa agaggaaaga gagacaccca ccaccgacag catgcccccc
     6181 ctgctgaagc aggccatcga gctgttcgac agacagggcg ccgagcccgc cttcctgttc
     6241 ggcctggaac tgatcatctg cggcctcgag aagcagctga agtgcgagag cggcggaccc
     6301 accgacgccc tggacgactt cgacctggac atgctgcccg ccgatgccct ggatgatttt
     6361 gatctggata tgctccctgc tgacgctctc gatgacttcg atctcgatat gctgccaggc
     6421 gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg ccccgtgtct
     6481 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     6541 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     6601 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     6661 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     6721 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     6781 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     6841 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     6901 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     6961 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
     7021 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     7081 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     7141 agatactgcg acctccctag caaactgggg cacaagctta attccggact cagatctcga
     7201 gctgatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa
     7261 aagaagagaa aggtaggatc caccggatct agataactga tcataatcag ccataccaca
     7321 tttgaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7381 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7441 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7501 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7561 ggctctatgg ctagccgctg caagaactct tcctcacgat aacttcgtat aggagacttt
     7621 atacgaagtt aagcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     7801 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     7861 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7921 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7981 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8041 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8101 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8161 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     8221 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8281 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     8341 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8401 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8461 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8521 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8581 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8881 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8941 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9001 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9061 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9121 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9181 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9241 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9301 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9361 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9421 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9481 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9541 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9601 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9661 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9721 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9781 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9841 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9901 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9961 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10021 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10081 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10141 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10201 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10261 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10321 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10381 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10441 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10501 gaagcggaag
//
