LOCUS       Exported               11107 bp ds-DNA     circular SYN 17-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="from myobacteriumphage bxb1 (Thomson et al 2012)"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1925..2251
                     /label=8xDBM
     regulatory      2353..2362
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    2359..3387
                     /label=ETV2
     misc_feature    3386..3974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    4107..5700
                     /label=pCAG
     regulatory      5762..5767
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5778..7892
                     /codon_start=1
                     /label=synZiFTR
                     /translation="MSRPGERPFQCRICMRNFSRRHGLDRHTRTHTGEKPFQCRICMRN
                     FSDHSSLKRHLRTHTGSQKPFQCRICMRNFSVRHNLTRHLRTHTGEKPFQCRICMRNFS
                     DHSNLSRHLKTHTGSQKPFQCRICMRNFSQRSSLVRHLRTHTGEKPFQCRICMRNFSES
                     GHLKRHLRTHLRGSTCRDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPGGLEG
                     GGGSGGTEDVVCCHSIYGKKKGDIDTYRYIGSSGTGCVVIVGRIVLSGSGTSAPITAYA
                     QQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWAVYHGAGTRTIAS
                     PKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGS
                     LLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVF
                     TDNSSPPAVTLTHPITKIDREVLYQEFDEMEECSQHYPYDVPDYAGGGGSGGTDEFPTM
                     VFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPA
                     PKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPV
                     APHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFS
                     ALLSQISSQL"
     misc_feature    5778..6317
                     /label=ZF10
     CDS             6318..6383
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6390..6410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6411..7259
                     /label=NS3
     CDS             7260..7286
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    7512..7883
                     /label=p65
     polyA_signal    7893..8117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8151..8198
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      8465..8920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9202..9306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9307..10167
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10338..10926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagcggacga ttgagtaaca ctgtatctag
     1921 tctacctaca gggctcggcg tagccgatgt cgcgcggata gcgtcaacct cttcggcgta
     1981 gccgatgtcg cgcaatcagt tctcccgagg tcggcgtagc cgatgtcgcg cagagccgtg
     2041 cttccaatac ggcgtagccg atgtcgcgcc gggtttcgta acaatcgcat gaggattcgc
     2101 aacgccttcg gcgtagccga tgtcgcgctc ccgtctcagt aaaggtcggc gtagccgatg
     2161 tcgcgcaatc ggactgcctt cgtacggcgt agccgatgtc gcgcgtatca gtcgcctcgg
     2221 aacggcgtag ccgatgtcgc gcattcgtaa gaggctcact ctcccttaca cggagtggat
     2281 aggatcctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
     2341 cgctaccgga tcgccaccat ggacctgtgg aactgggatg aggcatcccc acaggaagtg
     2401 cctccaggga acaagctggc agggcttgaa ggagccaaat taggcttctg tttccctgat
     2461 ctggcactcc aaggggacac gccgacagcg acagcagaga catgctggaa aggtacaagc
     2521 tcatccctgg caagcttccc acagctggac tggggctccg cgttactgca cccagaagtt
     2581 ccatgggggg cggagcccga ctctcaggct cttccgtggt ccggggactg gacagacatg
     2641 gcgtgcacag cctgggactc ttggagcggc gcctcgcaga ccctgggccc cgcccctctc
     2701 ggcccgggcc ccatccccgc cgccggctcc gaaggcgccg cgggccagaa ctgcgtcccc
     2761 gtggcgggag aggccacctc gtggtcgcgc gcccaggccg ccgggagcaa caccagctgg
     2821 gactgttctg tggggcccga cggcgatacc tactggggca gtggcctggg cggggagccg
     2881 cgcacggact gtaccatttc gtggggcggg cccgcgggcc cggactgtac cacctcctgg
     2941 aacccggggc tgcatgcggg tggcaccacc tctttgaagc ggtaccagag ctcagctctc
     3001 accgtttgct ccgaaccgag cccgcagtcg gaccgtgcca gtttggctcg atgccccaaa
     3061 actaaccacc gaggtcccat tcagctgtgg cagttcctcc tggagctgct ccacgacggg
     3121 gcgcgtagca gctgcatccg ttggactggc aacagccgcg agttccagct gtgcgacccc
     3181 aaagaggtgg ctcggctgtg gggcgagcgc aagagaaagc cgggcatgaa ttacgagaag
     3241 ctgagccggg gccttcgcta ctactatcgc cgcgacatcg tgcgcaagag cggggggcga
     3301 aagtacacgt accgcttcgg gggccgcgtg cccagcctag cctatccgga ctgtgcggga
     3361 ggcggacggg gagcagagac acaataatca acctctggat tacaaaattt gtgaaagatt
     3421 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3481 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3541 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3601 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3661 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3781 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3841 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3901 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3961 ggccgcctcc ccgccctttg ggccgcctcc ccgcgctagt gctggggatg cggtgggctc
     4021 tatgggtctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4081 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4141 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4201 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4261 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4321 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4381 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     4441 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     4501 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
     4561 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     4621 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     4681 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
     4741 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     4801 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
     4861 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
     4921 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
     4981 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     5041 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     5101 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     5161 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5221 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5281 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     5341 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     5401 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     5461 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     5521 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     5581 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     5641 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     5701 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attccgatcg
     5761 cgccacctgc cgccaccatg tctagacccg gagagcgccc attccagtgt cggatttgca
     5821 tgcggaactt ttcgagaaga cacggcctgg acagacatac ccgtactcat acaggtgaaa
     5881 aaccctttca gtgtcggatc tgtatgcgaa atttctccga ccacagcagc ctgaagagac
     5941 atctacgtac ccacaccggc agccagaagc catttcagtg tcggatctgt atgcggaact
     6001 tctccgtgag acacaacctg accagacatc tacgtacgca caccggagag aagccattcc
     6061 aatgccgaat atgcatgcgc aacttcagtg accacagcaa cctgagcaga cacctaaaaa
     6121 cccacaccgg ttcccagaag ccatttcagt gtcggatctg tatgcggaac ttctcccagc
     6181 gcagcagcct ggtgagacat ctacgtacgc acaccggaga gaagccattc caatgccgaa
     6241 tatgcatgcg caacttcagt gagagcggcc acctgaagag acacctgcgt acgcacctga
     6301 ggggatccac ctgcagggac tacaaagacc atgacggtga ttataaagat catgacatcg
     6361 attacaagga tgacgatgac aagatggccc ccaagaaaaa gaggaaggtg ggcattcacg
     6421 gggtgccggg tggactcgag ggaggcggtg gaagcggcgg taccgaggac gtggtgtgct
     6481 gccactcaat ctacggcaag aagaagggtg atatcgacac ctaccgatac ataggctctt
     6541 ccgggacagg ctgcgtggtc atagtgggca ggatcgtctt gtccggatcc ggcactagtg
     6601 cgcccatcac ggcgtacgcc cagcagacga gaggcctcct agggtgtata atcaccagcc
     6661 tgactggccg ggacaaaaac caagtggagg gtgaggtcca gatcgtgtca actgctaccc
     6721 aaaccttcct ggcaacgtgc atcaatgggg tatgctgggc agtctaccac ggggccggaa
     6781 cgaggaccat cgcatcaccc aagggtcctg tcatccagat gtataccaat gtggaccaag
     6841 accttgtggg ctggcccgct cctcaaggtt cccgctcatt gacaccctgt acctgcggct
     6901 cctcggacct ttacctggtc acgaggcacg ccgatgtcat tcccgtgcgc cggcgaggtg
     6961 atagcagggg tagcctgctt tcgccccggc ccatttccta cttgaaaggc tcctcggggg
     7021 gtccgctgtt gtgccccgcg ggacacgccg tgggcctatt cagggccgcg gtgtgcaccc
     7081 gtggagtggc taaagcggtg gactttatcc ctgtggagaa cctagagaca accatgagat
     7141 ccccggtgtt cacggacaac tcctctccac cagcagtcac cctgacgcac ccaatcacca
     7201 aaatcgatag ggaggttctc taccaggagt tcgatgagat ggaagagtgc tctcagcact
     7261 atccctacga tgtgcccgat tacgctggag gcggtggaag cggcggtacc gatgagtttc
     7321 ccaccatggt gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc
     7381 cccaagtcct gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg
     7441 cccaggcccc agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac
     7501 ctgcccccaa gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc
     7561 agtttgatga tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca
     7621 cagacctggc atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg
     7681 tggcccccca cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag
     7741 tgacaggggc ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc
     7801 ccaatggcct cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag
     7861 ccctgctgag tcagatcagc tcccaattgt aactgtgcct tctagttgcc agccatctgt
     7921 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7981 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     8041 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     8101 tgcggtgggc tctatggcta gccgctgcaa gaactcttcc tcacggatcc gaagttccta
     8161 ttccgaagtt cctattcttc aaatagtata ggaacttcag cgctcactgg ccgtcgtttt
     8221 acaaagggcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8281 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     8401 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     8461 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     8701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     8821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8881 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     8941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     9001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     9061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     9121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9541 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9841 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    10501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11041 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11101 gcggaag
//
