LOCUS       Exported               11892 bp ds-DNA     circular SYN 17-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="mutant attB recombination sequence for bxb1"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1894..2287
                     /label=TRE-3G
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1936..1954
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1972..1990
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2008..2026
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2044..2062
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2080..2098
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     regulatory      2282..2287
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2288..4627
                     /label=hNIL
     CDS             3359..3412
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4628..5216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    5273..6950
                     /label=pCAG
     misc_feature    7009..7752
                     /label=Tet-On(R) 3G
     CDS             7753..7806
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7807..8514
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             8539..8559
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8563..8583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8587..8607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8678..8902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8936..8983
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      9250..9705
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9987..10091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10092..10952
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11123..11711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aaggtttact ccctatcagt gatagagaac
     1921 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     1981 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     2041 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     2101 gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt
     2161 gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac
     2221 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa cttaagaatt
     2281 cgccaccatg ggagacatgg gagatccacc aaaaaaaaaa cgtctgattt ccctatgtgt
     2341 tggttgcggc aatcagattc acgatcagta tattctgagg gtttctccgg atttggaatg
     2401 gcatgcggca tgtttgaaat gtgcggagtg taatcagtat ttggacgaga gctgtacatg
     2461 ctttgttagg gatgggaaaa cctactgtaa aagagattat atcaggttgt acgggatcaa
     2521 atgcgccaag tgcagcatcg gcttcagcaa gaacgacttc gtgatgcgtg cccgctccaa
     2581 ggtgtatcac atcgagtgtt tccgctgtgt ggcctgcagc cgccagctca tccctgggga
     2641 cgaatttgcg cttcgggagg acggtctctt ctgccgagca gaccacgatg tggtggagag
     2701 ggccagtcta ggcgctggcg acccgctcag tcccctgcat ccagcgcggc cactgcaaat
     2761 ggcagcggag cccatctccg ccaggcagcc ggccctgcgg ccccacgtcc acaagcagcc
     2821 ggagaagacc acccgcgtgc ggactgtgct gaacgagaag cagctgcaca ccttgcggac
     2881 ctgctacgcc gcaaacccgc ggccagatgc gctcatgaag gagcaactgg tagagatgac
     2941 gggcctcagt ccccgtgtga tccgggtctg gtttcaaaac aagcggtgca aggacaagaa
     3001 gcgaagcatc atgatgaagc aactccagca gcagcagccc aatgacaaaa ctaatatcca
     3061 ggggatgaca ggaactccca tggtggctgc cagtccagag agacacgacg gtggcttaca
     3121 ggctaaccca gtggaagtac aaagttacca gccaccttgg aaagtactga gcgacttcgc
     3181 cttgcagagt gacatagatc agcctgcttt tcagcaactg gtcaattttt cagaaggagg
     3241 accgggctct aattccactg gcagtgaagt agcatcaatg tcctctcaac ttccagatac
     3301 acctaacagc atggtagcca gtcctattga ggcattgatg cacagcagag gctctggcga
     3361 gggcagaggc agcctgctga catgtggcga cgtggaagag aaccctggcc ccggtaccat
     3421 ggaagccaga ggcgaactgg gccctgccag agaatctgcc ggcggagatc tgctgctggc
     3481 cctgctggct agaagggccg acctgagaag agaaatcccc ctgtgcgccg gctgcgacca
     3541 gcacatcctg gaccggttta tcctgaaggc cctggacaga cactggcaca gcaagtgcct
     3601 gaagtgcagc gactgccaca cccccctggc cgagagatgc tttagcagag gcgagagcgt
     3661 gtactgcaag gacgacttct tcaagagatt cggcaccaag tgcgccgcct gccagctggg
     3721 aattcctcct acacaggtcg tgcggagagc ccaggacttc gtgtaccatc tgcactgctt
     3781 cgcctgcgtc gtgtgcaaga gacagctggc caccggcgac gagttctacc tgatggaaga
     3841 tagccggctc gtgtgtaaag ccgactacga gacagccaag cagagagagg ccgaggccac
     3901 cgccaaaaga cccagaacca ccatcaccgc caagcagctg gaaaccctga agtccgccta
     3961 caacaccagc cccaagcccg ccagacatgt gcgggaacag ctgagcagcg agacaggcct
     4021 ggacatgaga gtggtgcaag tgtggttcca gaacagacgg gccaaagaga agcggctgaa
     4081 gaaggacgcc ggcaggcaga gatggggcca gtacttccgg aacatgaagc ggtccagagg
     4141 cggcagcaag agcgacaagg acagcgtgca ggaaggccag gacagcgacg ccgaggtgtc
     4201 cttcccagat gagcctagcc tggccgaaat gggcccagcc aatggcctgt atggcagcct
     4261 gggagagcca acccaggctc tgggcagacc ttctggcgcc ctgggcaact tctctctgga
     4321 acacggcgga ctggccggac ccgagcagta tagagaactg aggcccggca gcccttacgg
     4381 cgtgccacct tctccagctg cccctcagtc tctgccagga cctcagcctc tgctgtccag
     4441 cctggtgtac cccgatacaa gcctgggcct ggtgccatct ggcgctccag gcggacctcc
     4501 tccaatgaga gtgctggccg gcaatggccc tagcagcgat ctgagcacag gcagctctgg
     4561 cggctacccc gattttcctg ccagccctgc ctcctggctg gacgaagtgg atcacgccca
     4621 gttctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4681 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4741 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4801 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4861 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4921 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4981 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5041 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5101 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5161 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgccctt
     5221 tgggccgcct ccccgcgcta gtgctgggga tgcggtgggc tctatgggtc tcgacattga
     5281 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5641 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     5701 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     5761 gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     5821 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     5881 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc
     5941 tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     6001 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     6061 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     6121 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     6181 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     6241 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     6301 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     6361 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     6421 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     6481 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     6541 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     6601 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     6661 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     6721 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     6781 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     6841 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     6901 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     6961 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga atgtcgacat gagccggctg
     7021 gacaagagca aagtgatcaa cagcgccctg gaactgctga acggcgtggg catcgagggc
     7081 ctgaccaccc ggaagctggc ccagaaactg ggcgtggaac agcccaccct gtactggcac
     7141 gtgaagaaca agcgggccct gctggacgcc ctgcccatcg agatgctgga ccggcaccac
     7201 acccacagct gccctctgga aggcgagagc tggcaggact tcctgcggaa caacgccaag
     7261 agctacagat gcgccctgct gagccaccgg gacggcgcca aagtgcacct gggcaccaga
     7321 cccaccgaga agcagtacga gacactggaa aaccagctgg ccttcctgtg ccagcagggc
     7381 ttcagcctgg aaaacgccct gtacgccctg agcgccgtgg gccacttcac cctgggctgc
     7441 gtgctggaag aacaggaaca ccaggtcgcc aaagaggaaa gagagacacc caccaccgac
     7501 agcatgcccc ccctgctgaa gcaggccatc gagctgttcg acagacaggg cgccgagccc
     7561 gccttcctgt tcggcctgga actgatcatc tgcggcctcg agaagcagct gaagtgcgag
     7621 agcggcggac ccaccgacgc cctggacgac ttcgacctgg acatgctgcc cgccgatgcc
     7681 ctggatgatt ttgatctgga tatgctccct gctgacgctc tcgatgactt cgatctcgat
     7741 atgctgccag gcgagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc
     7801 ggccccgtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
     7861 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     7921 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     7981 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     8041 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     8101 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     8161 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     8221 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     8281 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     8341 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     8401 gactacagac tggaaagaat caaggaggcc aacaacgaga catacgtcga gcagcacgag
     8461 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga
     8521 ctcagatctc gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     8581 gtagatccaa aaaagaagag aaaggtagga tccaccggat ctagataact gatcataatc
     8641 agccatacca catttgaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     8701 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8761 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8821 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8881 ggggatgcgg tgggctctat ggctagccgc tgcaagaact cttcctcacg gatccgaagt
     8941 tcctattccg aagttcctat tcttcaaata gtataggaac ttcagcgctc actggccgtc
     9001 gttttacaaa gggcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     9061 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     9121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     9181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     9241 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     9301 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     9361 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9421 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     9481 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9541 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     9601 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     9661 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     9721 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     9781 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9841 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     9901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    11281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11881 aggaagcgga ag
//
