LOCUS       Exported               12708 bp ds-DNA     circular SYN 13-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="from myobacteriumphage bxb1 (Thomson et al 2012)"
     protein_bind    1802..1835
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1875..2268
                     /label=TRE-3G
     protein_bind    1881..1899
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1917..1935
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1953..1971
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1989..2007
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2025..2043
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2061..2079
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2097..2115
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     promoter        2242..2251
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     regulatory      2263..2268
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2269..5493
                     /label=hNIL
     CDS             3097..3153
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4225..4278
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5494..6082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    6139..7816
                     /label=pCAG
     misc_feature    7875..8618
                     /label=Tet-On(R) 3G
     CDS             8619..8672
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8673..9380
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             9405..9425
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9429..9449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9453..9473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9544..9768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9797..9829
                     /label=lox251
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     rep_origin      10066..10521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10803..10907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10908..11768
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11939..12527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggtctccg tcgtcaggat catccggatc
     1801 cataacttcg tatagcatac attatacgaa gttatcatga tattcggcaa gcaggcatcg
     1861 actagttaat taaggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1921 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1981 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     2041 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     2101 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
     2161 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
     2221 aactttccgt accacttcct accctcgtaa acttaagaat tcgccaccat gttcgtcaaa
     2281 tccgagacct tggagttgaa ggaggaagag gacgtgttag tgctgctcgg atcggcctcc
     2341 cccgccttgg cggccctgac cccgctgtca tccagcgccg acgaagaaga ggaggaggag
     2401 ccgggcgcgt caggcggggc gcgtcggcag cgcggggctg aggccgggca gggggcgcgg
     2461 ggcggcgtgg ctgcgggtgc ggagggctgc cggcccgcac ggctgctggg tctggtacac
     2521 gattgcaaac ggcgcccttc ccgggcgcgg gccgtctccc gaggcgccaa gacggccgag
     2581 acggtgcagc gcatcaagaa gacccgtaga ctgaaggcca acaaccgcga gcgaaaccgc
     2641 atgcacaacc tcaacgcggc actggacgcg ctgcgcgagg tgctccccac gttccccgag
     2701 gacgccaagc tcaccaagat cgagaccctg cgcttcgccc acaactacat ctgggcactc
     2761 accgagaccc tgcgcctggc ggatcactgc gggggcggcg gcgggggcct gccgggggcg
     2821 ctcttctccg aggcagtgtt gctgagcccg ggaggcgcca gcgccgccct gagcagcagc
     2881 ggagacagcc cctcgcccgc ctccacgtgg agttgcacca acagccccgc gccgtcctcc
     2941 tccgtgtcct ccaattccac ctccccctac agctgcactt tatcgcccgc cagcccggcc
     3001 gggtcagaca tggactattg gcagccccca cctcccgaca agcaccgcta tgcacctcac
     3061 ctccccatag ccagggattg tatcttggga tccggagcta ctaacttcag cctgctgaag
     3121 caggctggag acgtggagga gaaccctgga cctatgggag acatgggaga tccaccaaaa
     3181 aaaaaacgtc tgatttccct atgtgttggt tgcggcaatc agattcacga tcagtatatt
     3241 ctgagggttt ctccggattt ggaatggcat gcggcatgtt tgaaatgtgc ggagtgtaat
     3301 cagtatttgg acgagagctg tacatgcttt gttagggatg ggaaaaccta ctgtaaaaga
     3361 gattatatca ggttgtacgg gatcaaatgc gccaagtgca gcatcggctt cagcaagaac
     3421 gacttcgtga tgcgtgcccg ctccaaggtg tatcacatcg agtgtttccg ctgtgtggcc
     3481 tgcagccgcc agctcatccc tggggacgaa tttgcgcttc gggaggacgg tctcttctgc
     3541 cgagcagacc acgatgtggt ggagagggcc agtctaggcg ctggcgaccc gctcagtccc
     3601 ctgcatccag cgcggccact gcaaatggca gcggagccca tctccgccag gcagccggcc
     3661 ctgcggcccc acgtccacaa gcagccggag aagaccaccc gcgtgcggac tgtgctgaac
     3721 gagaagcagc tgcacacctt gcggacctgc tacgccgcaa acccgcggcc agatgcgctc
     3781 atgaaggagc aactggtaga gatgacgggc ctcagtcccc gtgtgatccg ggtctggttt
     3841 caaaacaagc ggtgcaagga caagaagcga agcatcatga tgaagcaact ccagcagcag
     3901 cagcccaatg acaaaactaa tatccagggg atgacaggaa ctcccatggt ggctgccagt
     3961 ccagagagac acgacggtgg cttacaggct aacccagtgg aagtacaaag ttaccagcca
     4021 ccttggaaag tactgagcga cttcgccttg cagagtgaca tagatcagcc tgcttttcag
     4081 caactggtca atttttcaga aggaggaccg ggctctaatt ccactggcag tgaagtagca
     4141 tcaatgtcct ctcaacttcc agatacacct aacagcatgg tagccagtcc tattgaggca
     4201 ttgatgcaca gcagaggctc tggcgagggc agaggcagcc tgctgacatg tggcgacgtg
     4261 gaagagaacc ctggccccgg taccatggaa gccagaggcg aactgggccc tgccagagaa
     4321 tctgccggcg gagatctgct gctggccctg ctggctagaa gggccgacct gagaagagaa
     4381 atccccctgt gcgccggctg cgaccagcac atcctggacc ggtttatcct gaaggccctg
     4441 gacagacact ggcacagcaa gtgcctgaag tgcagcgact gccacacccc cctggccgag
     4501 agatgcttta gcagaggcga gagcgtgtac tgcaaggacg acttcttcaa gagattcggc
     4561 accaagtgcg ccgcctgcca gctgggaatt cctcctacac aggtcgtgcg gagagcccag
     4621 gacttcgtgt accatctgca ctgcttcgcc tgcgtcgtgt gcaagagaca gctggccacc
     4681 ggcgacgagt tctacctgat ggaagatagc cggctcgtgt gtaaagccga ctacgagaca
     4741 gccaagcaga gagaggccga ggccaccgcc aaaagaccca gaaccaccat caccgccaag
     4801 cagctggaaa ccctgaagtc cgcctacaac accagcccca agcccgccag acatgtgcgg
     4861 gaacagctga gcagcgagac aggcctggac atgagagtgg tgcaagtgtg gttccagaac
     4921 agacgggcca aagagaagcg gctgaagaag gacgccggca ggcagagatg gggccagtac
     4981 ttccggaaca tgaagcggtc cagaggcggc agcaagagcg acaaggacag cgtgcaggaa
     5041 ggccaggaca gcgacgccga ggtgtccttc ccagatgagc ctagcctggc cgaaatgggc
     5101 ccagccaatg gcctgtatgg cagcctggga gagccaaccc aggctctggg cagaccttct
     5161 ggcgccctgg gcaacttctc tctggaacac ggcggactgg ccggacccga gcagtataga
     5221 gaactgaggc ccggcagccc ttacggcgtg ccaccttctc cagctgcccc tcagtctctg
     5281 ccaggacctc agcctctgct gtccagcctg gtgtaccccg atacaagcct gggcctggtg
     5341 ccatctggcg ctccaggcgg acctcctcca atgagagtgc tggccggcaa tggccctagc
     5401 agcgatctga gcacaggcag ctctggcggc taccccgatt ttcctgccag ccctgcctcc
     5461 tggctggacg aagtggatca cgcccagttc tgaaatcaac ctctggatta caaaatttgt
     5521 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5581 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5641 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5701 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5761 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5821 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5881 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5941 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6001 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6061 tccctttggg ccgcctcccc gccctttggg ccgcctcccc gcgctagtgc tggggatgcg
     6121 gtgggctcta tgggtctcga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     6481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     6541 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     6601 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
     6661 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     6721 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     6781 gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc
     6841 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     6901 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     6961 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     7021 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     7081 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     7141 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     7201 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     7261 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     7321 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     7381 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     7441 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     7501 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     7561 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     7621 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     7681 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     7741 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     7801 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     7861 caaagaatgt cgacatgagc cggctggaca agagcaaagt gatcaacagc gccctggaac
     7921 tgctgaacgg cgtgggcatc gagggcctga ccacccggaa gctggcccag aaactgggcg
     7981 tggaacagcc caccctgtac tggcacgtga agaacaagcg ggccctgctg gacgccctgc
     8041 ccatcgagat gctggaccgg caccacaccc acagctgccc tctggaaggc gagagctggc
     8101 aggacttcct gcggaacaac gccaagagct acagatgcgc cctgctgagc caccgggacg
     8161 gcgccaaagt gcacctgggc accagaccca ccgagaagca gtacgagaca ctggaaaacc
     8221 agctggcctt cctgtgccag cagggcttca gcctggaaaa cgccctgtac gccctgagcg
     8281 ccgtgggcca cttcaccctg ggctgcgtgc tggaagaaca ggaacaccag gtcgccaaag
     8341 aggaaagaga gacacccacc accgacagca tgccccccct gctgaagcag gccatcgagc
     8401 tgttcgacag acagggcgcc gagcccgcct tcctgttcgg cctggaactg atcatctgcg
     8461 gcctcgagaa gcagctgaag tgcgagagcg gcggacccac cgacgccctg gacgacttcg
     8521 acctggacat gctgcccgcc gatgccctgg atgattttga tctggatatg ctccctgctg
     8581 acgctctcga tgacttcgat ctcgatatgc tgccaggcga gggcagggga agtcttctaa
     8641 catgcgggga cgtggaggaa aatcccggcc ccgtgtctaa gggcgaagag ctgattaagg
     8701 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
     8761 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     8821 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
     8881 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
     8941 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     9001 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
     9061 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
     9121 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
     9181 ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc gctaagaacc
     9241 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
     9301 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
     9361 aactggggca caagcttaat tccggactca gatctcgagc tgatccaaaa aagaagagaa
     9421 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtaggatcca
     9481 ccggatctag ataactgatc ataatcagcc ataccacatt tgaaacccgc tgatcagcct
     9541 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9601 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9661 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9721 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct agccgctgca
     9781 agaactcttc ctcacgataa cttcgtatag gagactttat acgaagttaa gcgctcactg
     9841 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9901 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9961 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
    10021 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
    10081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10261 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
    10321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10381 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
    10441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10501 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
    10561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    10621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    10681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    10741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    10801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    11041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    11101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    12001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    12061 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    12121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
