LOCUS       Exported                9920 bp ds-DNA     circular SYN 25-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MLLn-CAGEn-NES (Npu)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Meyers S, Lenny N, Hiebert SW
  TITLE     The t(8;21) fusion protein interferes with AML-1B-dependent 
            transcriptional activation.
  JOURNAL   Mol Cell Biol. 1995 Apr . 15(4):1974-82.
  PUBMED    7891692
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     833..849
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     833..849
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6121..6743
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6320..6339)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6767..6787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6772..7101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6952..7087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7014..7033
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7037..7048)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7139..7158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7140..7158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7172..7188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7172..7188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7185..7207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7220..7250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7403..7420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7574..8162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7654..7673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8333..9193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8956..8975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9194..9298)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9325..9780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9412..9431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9622..9643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(9906..9920,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9920,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcagatct ggtaccacgc gtatcgataa gcttgcatgc ctgcaggtcg actctagaac
      841 tagtggatcc gttgtgatgg cgcacagctg tcggtggcgc ttccccgccc gacccgggac
      901 caccgggggc ggcggcggcg gggggcgccg gggcctaggg ggcgccccgc ggcaacgcgt
      961 cccggccctg ctgcttcccc ccgggccccc ggtcggcggt ggcggccccg gggcgccccc
     1021 ctcccccccg gctgtggcgg ccgcggcggc ggcggcggga agcagcgggg ctggggttcc
     1081 agggggagcg gccgccgcct cagcagcctc ctcgtcgtcc gcctcgtctt cgtcttcgtc
     1141 atcgtcctca gcctcttcag ggccggccct gctccgggtg ggcccgggct tcgacgcggc
     1201 gctgcaggtc tcggccgcca tcggcaccaa cctgcgccgg ttccgggccg tgtttgggga
     1261 gagcggcggg ggaggcggca gcggagagga tgagcaattc ttaggttttg gctcagatga
     1321 agaagtcaga gtgcgaagtc ccacaaggtc tccttcagtt aaaactagtc ctcgaaaacc
     1381 tcgtgggaga cctagaagtg gctctgaccg aaattcagct atcctctcag atccatctgt
     1441 gttttcccct ctaaataaat cagagaccaa atctggagat aagatcaaga agaaagattc
     1501 taaaagtata gaaaagaaga gaggaagacc tcccaccttc cctggagtaa aaatcaaaat
     1561 aacacatgga aaggacattt cagagttacc aaagggaaac aaagaagata gcctgaaaaa
     1621 aattaaaagg acaccttctg ctacgtttca gcaagccaca aagattaaaa aattaagagc
     1681 aggtaaactc tctcctctca agtctaagtt taagacaggg aagcttcaaa taggaaggaa
     1741 gggggtacaa attgtacgac ggagaggaag gcctccatca acagaaagga taaagacccc
     1801 ttcgggtctc ctcattaatt ctgaactgga aaagccccag aaagtccgga aagacaagga
     1861 aggaacacct ccacttacaa aagaagataa gacagttgtc agacaaagcc ctcgaaggat
     1921 taagccagtt aggattattc cttcttcaaa aaggacagat gcaaccattg ctaagcaact
     1981 cttacagagg gcaaaaaagg gggctcaaaa gaaaattgaa aaagaagcag ctcagctgca
     2041 gggaagaaag gtgaagacac aggtcaaaaa tattcgacag ttcatcatgc ctgttgtcag
     2101 tgctatctcc tcgcggatca ttaagacccc tcggcggttt atagaggatg aggattatga
     2161 ccctccaatt aaaattgccc gattagagtc tacaccgaat agtagattca gtgccccgtc
     2221 ctgtggatct tctgaaaaat caagtgcagc ttctcagcac tcctctcaaa tgtcttcaga
     2281 ctcctctcga tctagtagcc ccagtgttga tacctccaca gactctcagg cttctgagga
     2341 gattcaggta cttcctgagg agcggagcga tacccctgaa gttcatcctc cactgcccat
     2401 ttcccagtcc ccagaaaatg agagtaatga taggagaagc agaaggtatt cagtgtcgga
     2461 gagaagtttt ggatctagaa cgacgaaaaa attatcaact ctacaaagtg ccccccagca
     2521 gcagacctcc tcgtctccac ctccacctct gctgactcca ccgccaccac tgcagccagc
     2581 ctccagtatc tctgaccaca caccttggct tatgcctcca acaatcccct tagcatcacc
     2641 atttttgcct gcttccactg ctcctatgca agggaagcga aaatctattt tgcgagaacc
     2701 gacatttagg tggacttctt taaagcattc taggtcagag ccacaatact tttcctcagc
     2761 aaagtatgcc aaagaaggtc ttattcgcaa accaatattt gataatttcc gaccccctcc
     2821 actaactccc gaggacgttg gctttgcatc tggtttttct gcatctggta ccgctgcttc
     2881 agcccgattg ttttcgccac tccattctgg aacaaggttt gatatgcaca aaaggagccc
     2941 tcttctgaga gctccaagat ttactccaag tgaggctcac tctagaatat ttgagtctgt
     3001 aaccttgcct agtaatcgaa cttctgctgg aacatcttct tcaggagtat ccaatagaaa
     3061 aaggaaaaga aaagtgttta gtcctattcg atctgaacca agatctcctt ctcactccat
     3121 gaggacaaga agtggaaggc ttagtagttc tgagctctca cctctcaccc ccccgtcttc
     3181 tgtctcttcc tcgttaagca tttctgttag tcctcttgcc actagtgcct taaacccaac
     3241 ttttactttt ccttctcatt ccctgactca gtctggggaa tctgcagaga aaaatcagag
     3301 accaaggaag cagactagtg ctccggcaga gccattttca tcaagtagtc ctactcctct
     3361 cttcccttgg tttaccccag gctctcagac tgaaagaggg agaaataaag acaaggcccc
     3421 cgaggagctg tccaaagatc gagatgctga caagagcgtg gagaaggaca agagtagaga
     3481 gagagaccgg gagagagaaa aggagaataa gcgggagtca aggaaagaga aaaggaaaaa
     3541 gggatcagaa attcagagta gttctgcttt gtatcctgtg ggtagggttt ccaaagagaa
     3601 ggttgttggt gaagatgttg ccacttcatc ttctgccaaa aaagcaacag ggcggaagaa
     3661 gtcttcatca catgattctg ggactgatat tacttctgtg actcttgggg atacaacagc
     3721 tgtcaaaacc aaaatactta taaagaaagg gagaggaaat ctggaaaaaa ccaacttgga
     3781 cctcggccca actgccccat ccctggagaa ggagaaaacc ctctgccttt ccactccttc
     3841 atctagcact gttaaacatt ccacttcctc cataggctcc atgttggctc aggcagacaa
     3901 gcttccaatg actgacaaga gggttgccag cctcctaaaa aaggccaaag ctcagctctg
     3961 caagattgag aagagtaaga gtcttaaaca aaccgaccag cccaaagcac agggtcaaga
     4021 aagtgactca tcagagacct ctgtgcgagg accccggatt aaacatgtct gcagaagagc
     4081 agctgttgcc cttggccgaa aacgagctgt gtttcctgat gacatgccca ccctgagtgc
     4141 cttaccatgg gaagaacgag aaaagatttt gtcttccatg gggaatgatg acaagtcatc
     4201 aattgctggc tcagaagatg ctgaacctct tgctccaccc atcaaaccaa ttaaacctgt
     4261 cactagaaac aaggcacccc aggaacctcc agtaaagaaa ggacgtcgat cgaggcggtg
     4321 tgggcagtgt cccggctgcc aggtgcctga ggactgtggt gtttgtacta attgcttaga
     4381 taagcccaag tttggtggtc gcaatataaa gaagcagtgc tgcaagatga gaaaatgtca
     4441 gaatctacaa tggatgcctt ccaaagccta cctgcagaag caagctaaag ctgtgaaaaa
     4501 gaaagagaaa aagtctaaga ccagtgaaaa gaaagacagc aaagagagca gtgttgtgaa
     4561 gaacgtggtg gactctagtc agaaacctac cccatcagca agagaggatc ctgccccaaa
     4621 gaaaagcagt agtgagcctc ctccacgaaa gcccgtcgag gaaaagagtg aagaagggaa
     4681 tgtctcggcc cctgggcctg aatccaaaca ggccaccact ccagcttcca ggaagtcaag
     4741 caagcaggtc tcccagccag cactggtcat cccgcctcag ccacctacta caggaccgcc
     4801 aagaaaagaa gttcccaaaa ccactcctag tgagcccaag aaaaagcagc ctccaccacc
     4861 agaatcaggt ccagagcaga gcaaacagaa aaaagtggct ccccgcccaa gtatccctgt
     4921 aaaacaaaaa ccaaaagaaa aggaaaaacc acctccggtc aataagcagg agaatgcagg
     4981 cactttgaac atcctcagca ctctctccaa tggcaatagt tctaagcaaa aaattccagc
     5041 agatggagtc cacaggatca gagtggactt taaggaagac tgtgaagcag aaaatgtgtg
     5101 ggagatggga ggcttaggga tctgtttaag ctatgaaacg gaaatattga cagtagaata
     5161 tggattatta ccgattggta aaattgtaga aaagcgcatc gaatgtactg tttatagcgt
     5221 tgataataat ggaaatattt atacacaacc tgtagcacaa tggcacgatc gcggaaagca
     5281 aaaggtgttt gagtattgtt tggaggatgg ttcattgatt cgggcaacaa aagaccataa
     5341 gtttatgact gttgatggtc aaatgttgcc aattaaggaa atatttcgtc gtaagttgga
     5401 tttgatgcgg gttgataatt tgccgaatgg aagcggagga gaaaatctat acttccaagg
     5461 tgagaacctg tattttcagg gcgggagtgg tgggatcgaa atagccacag aaaaatattt
     5521 aggcgaacaa aatgtctatg acattggagt tgagcgcgac cataattttg cactcaaaaa
     5581 tggcggctat tttcagggca tcgaaatagc cacagaaaaa tatttaggcg aacaaaatgt
     5641 ctatgacatt ggagttgagc gcgaccataa ttttgcactc aaaaatggca acagcgccgg
     5701 cggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca
     5761 gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg
     5821 ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga
     5881 agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta
     5941 tgcctatggt gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga
     6001 tgtggagctt ctaaaactgg aagaacagaa actgattagt gaggaagatg gaggaagtgg
     6061 aggtagtgct ctccaaaaaa agttggaaga gcttgaactg gacgaacagt agaggatccc
     6121 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     6181 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     6241 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     6301 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     6361 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     6421 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     6481 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     6541 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     6601 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     6661 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     6721 actcagtaga tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag
     6781 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6841 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6901 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6961 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7021 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7081 ggaggcctag gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc
     7141 ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     7201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9301 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9361 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9421 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9481 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9541 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9601 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9661 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9721 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9781 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9841 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9901 gttttcccag tcacgacgtt
//
