LOCUS       Exported                9848 bp ds-DNA     circular SYN 25-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MLLn-CAGEn-NES (Nrdj1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Meyers S, Lenny N, Hiebert SW
  TITLE     The t(8;21) fusion protein interferes with AML-1B-dependent 
            transcriptional activation.
  JOURNAL   Mol Cell Biol. 1995 Apr . 15(4):1974-82.
  PUBMED    7891692
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 17, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     833..849
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     833..849
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6049..6671
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6248..6267)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6695..6715)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6700..7029
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6880..7015
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6942..6961
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6965..6976)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7067..7086)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7068..7086)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7100..7116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7100..7116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7113..7135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7124..7140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7148..7178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7193..7214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7331..7348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7502..8090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7582..7601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8261..9121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8884..8903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9122..9226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9253..9708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9340..9359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9550..9571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(9834..9848,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9848,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcagatct ggtaccacgc gtatcgataa gcttgcatgc ctgcaggtcg actctagaac
      841 tagtggatcc gttgtgatgg cgcacagctg tcggtggcgc ttccccgccc gacccgggac
      901 caccgggggc ggcggcggcg gggggcgccg gggcctaggg ggcgccccgc ggcaacgcgt
      961 cccggccctg ctgcttcccc ccgggccccc ggtcggcggt ggcggccccg gggcgccccc
     1021 ctcccccccg gctgtggcgg ccgcggcggc ggcggcggga agcagcgggg ctggggttcc
     1081 agggggagcg gccgccgcct cagcagcctc ctcgtcgtcc gcctcgtctt cgtcttcgtc
     1141 atcgtcctca gcctcttcag ggccggccct gctccgggtg ggcccgggct tcgacgcggc
     1201 gctgcaggtc tcggccgcca tcggcaccaa cctgcgccgg ttccgggccg tgtttgggga
     1261 gagcggcggg ggaggcggca gcggagagga tgagcaattc ttaggttttg gctcagatga
     1321 agaagtcaga gtgcgaagtc ccacaaggtc tccttcagtt aaaactagtc ctcgaaaacc
     1381 tcgtgggaga cctagaagtg gctctgaccg aaattcagct atcctctcag atccatctgt
     1441 gttttcccct ctaaataaat cagagaccaa atctggagat aagatcaaga agaaagattc
     1501 taaaagtata gaaaagaaga gaggaagacc tcccaccttc cctggagtaa aaatcaaaat
     1561 aacacatgga aaggacattt cagagttacc aaagggaaac aaagaagata gcctgaaaaa
     1621 aattaaaagg acaccttctg ctacgtttca gcaagccaca aagattaaaa aattaagagc
     1681 aggtaaactc tctcctctca agtctaagtt taagacaggg aagcttcaaa taggaaggaa
     1741 gggggtacaa attgtacgac ggagaggaag gcctccatca acagaaagga taaagacccc
     1801 ttcgggtctc ctcattaatt ctgaactgga aaagccccag aaagtccgga aagacaagga
     1861 aggaacacct ccacttacaa aagaagataa gacagttgtc agacaaagcc ctcgaaggat
     1921 taagccagtt aggattattc cttcttcaaa aaggacagat gcaaccattg ctaagcaact
     1981 cttacagagg gcaaaaaagg gggctcaaaa gaaaattgaa aaagaagcag ctcagctgca
     2041 gggaagaaag gtgaagacac aggtcaaaaa tattcgacag ttcatcatgc ctgttgtcag
     2101 tgctatctcc tcgcggatca ttaagacccc tcggcggttt atagaggatg aggattatga
     2161 ccctccaatt aaaattgccc gattagagtc tacaccgaat agtagattca gtgccccgtc
     2221 ctgtggatct tctgaaaaat caagtgcagc ttctcagcac tcctctcaaa tgtcttcaga
     2281 ctcctctcga tctagtagcc ccagtgttga tacctccaca gactctcagg cttctgagga
     2341 gattcaggta cttcctgagg agcggagcga tacccctgaa gttcatcctc cactgcccat
     2401 ttcccagtcc ccagaaaatg agagtaatga taggagaagc agaaggtatt cagtgtcgga
     2461 gagaagtttt ggatctagaa cgacgaaaaa attatcaact ctacaaagtg ccccccagca
     2521 gcagacctcc tcgtctccac ctccacctct gctgactcca ccgccaccac tgcagccagc
     2581 ctccagtatc tctgaccaca caccttggct tatgcctcca acaatcccct tagcatcacc
     2641 atttttgcct gcttccactg ctcctatgca agggaagcga aaatctattt tgcgagaacc
     2701 gacatttagg tggacttctt taaagcattc taggtcagag ccacaatact tttcctcagc
     2761 aaagtatgcc aaagaaggtc ttattcgcaa accaatattt gataatttcc gaccccctcc
     2821 actaactccc gaggacgttg gctttgcatc tggtttttct gcatctggta ccgctgcttc
     2881 agcccgattg ttttcgccac tccattctgg aacaaggttt gatatgcaca aaaggagccc
     2941 tcttctgaga gctccaagat ttactccaag tgaggctcac tctagaatat ttgagtctgt
     3001 aaccttgcct agtaatcgaa cttctgctgg aacatcttct tcaggagtat ccaatagaaa
     3061 aaggaaaaga aaagtgttta gtcctattcg atctgaacca agatctcctt ctcactccat
     3121 gaggacaaga agtggaaggc ttagtagttc tgagctctca cctctcaccc ccccgtcttc
     3181 tgtctcttcc tcgttaagca tttctgttag tcctcttgcc actagtgcct taaacccaac
     3241 ttttactttt ccttctcatt ccctgactca gtctggggaa tctgcagaga aaaatcagag
     3301 accaaggaag cagactagtg ctccggcaga gccattttca tcaagtagtc ctactcctct
     3361 cttcccttgg tttaccccag gctctcagac tgaaagaggg agaaataaag acaaggcccc
     3421 cgaggagctg tccaaagatc gagatgctga caagagcgtg gagaaggaca agagtagaga
     3481 gagagaccgg gagagagaaa aggagaataa gcgggagtca aggaaagaga aaaggaaaaa
     3541 gggatcagaa attcagagta gttctgcttt gtatcctgtg ggtagggttt ccaaagagaa
     3601 ggttgttggt gaagatgttg ccacttcatc ttctgccaaa aaagcaacag ggcggaagaa
     3661 gtcttcatca catgattctg ggactgatat tacttctgtg actcttgggg atacaacagc
     3721 tgtcaaaacc aaaatactta taaagaaagg gagaggaaat ctggaaaaaa ccaacttgga
     3781 cctcggccca actgccccat ccctggagaa ggagaaaacc ctctgccttt ccactccttc
     3841 atctagcact gttaaacatt ccacttcctc cataggctcc atgttggctc aggcagacaa
     3901 gcttccaatg actgacaaga gggttgccag cctcctaaaa aaggccaaag ctcagctctg
     3961 caagattgag aagagtaaga gtcttaaaca aaccgaccag cccaaagcac agggtcaaga
     4021 aagtgactca tcagagacct ctgtgcgagg accccggatt aaacatgtct gcagaagagc
     4081 agctgttgcc cttggccgaa aacgagctgt gtttcctgat gacatgccca ccctgagtgc
     4141 cttaccatgg gaagaacgag aaaagatttt gtcttccatg gggaatgatg acaagtcatc
     4201 aattgctggc tcagaagatg ctgaacctct tgctccaccc atcaaaccaa ttaaacctgt
     4261 cactagaaac aaggcacccc aggaacctcc agtaaagaaa ggacgtcgat cgaggcggtg
     4321 tgggcagtgt cccggctgcc aggtgcctga ggactgtggt gtttgtacta attgcttaga
     4381 taagcccaag tttggtggtc gcaatataaa gaagcagtgc tgcaagatga gaaaatgtca
     4441 gaatctacaa tggatgcctt ccaaagccta cctgcagaag caagctaaag ctgtgaaaaa
     4501 gaaagagaaa aagtctaaga ccagtgaaaa gaaagacagc aaagagagca gtgttgtgaa
     4561 gaacgtggtg gactctagtc agaaacctac cccatcagca agagaggatc ctgccccaaa
     4621 gaaaagcagt agtgagcctc ctccacgaaa gcccgtcgag gaaaagagtg aagaagggaa
     4681 tgtctcggcc cctgggcctg aatccaaaca ggccaccact ccagcttcca ggaagtcaag
     4741 caagcaggtc tcccagccag cactggtcat cccgcctcag ccacctacta caggaccgcc
     4801 aagaaaagaa gttcccaaaa ccactcctag tgagcccaag aaaaagcagc ctccaccacc
     4861 agaatcaggt ccagagcaga gcaaacagaa aaaagtggct ccccgcccaa gtatccctgt
     4921 aaaacaaaaa ccaaaagaaa aggaaaaacc acctccggtc aataagcagg agaatgcagg
     4981 cactttgaac atcctcagca ctctctccaa tggcaatagt tctaagcaaa aaattccagc
     5041 agatggagtc cacaggatca gagtggactt taaggaagac tgtgaagcag aaaatgtgtg
     5101 ggagatggga ggcttaggga tccttgaagt gaaaagtcca ataaagcaaa gcaaatgtct
     5161 ggtcggctct agcgaaatca tcacacgtaa ttatgggaag acaaccatta aggaagtagt
     5221 tgagatattt gacaatgata aaaatatcca agttctagcg tttaacactc acactgacaa
     5281 catcgagtgg gctcccatta aagctgcaca acttactaga ccgaacgctg agttggttga
     5341 actcgaaatt gataccctac atggtgtgaa aacgatcagg tgtacccccg accacccagt
     5401 ttacactaaa aacaggggct acgtgcgagc ggatgagtta accgatgatg atgagcttgt
     5461 tgtagccatc gagaacctgt atggcgatga agtagatggc tattttcagg gcatggaagc
     5521 ggagacgtat ataggaaagc ttaaatcccg agcaattgta tcaaacgagg acacgtatga
     5581 tattcagacg tcaactcata acttttttgc gaatgataac agcgccggcg gagtgcaggt
     5641 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt
     5701 gcactacacc gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa
     5761 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc
     5821 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc
     5881 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct
     5941 aaaactggaa gaacagaaac tgattagtga ggaagatgga ggaagtggag gtagtgctct
     6001 ccaaaaaaag ttggaagagc ttgaactgga cgaacagtag aggatcccgg gtggcatccc
     6061 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     6121 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     6181 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     6241 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     6301 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     6361 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     6421 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     6481 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     6541 ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg
     6601 ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg
     6661 cctgttgaat tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga
     6721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6781 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6841 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7021 ttttgcaaaa agctcctcga ggaactgaaa aaccagaaag ttaattccct atagtgagtc
     7081 gtattaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9241 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9661 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
     9721 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     9781 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     9841 acgacgtt
//
