LOCUS       Exported                6362 bp ds-DNA     circular SYN 27-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Orian Bricard
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     5..27
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     155..174
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
                     /note="color: black; sequence: GGTGATGTCGGCGATATAGG"
     LTR             374..890
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
                     /note="color: #ffe4c4"
     misc_feature    954..1295
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
                     /note="color: #ffe4c4"
     CDS             1362..1778
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1708..1730
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
                     /note="color: black; sequence: CCCTTGAACCTCCTCGTTCGACC"
     primer_bind     1714..1729
                     /label=MGFW
                     /note="color: black; sequence: aacctcctcgttcgac; added: 
                     2023-08-08"
     primer_bind     1746..1762
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
                     /note="color: black; sequence: CTTTATCCAGCCCTCAC"
     misc_feature    1756^1757
                     /label=start
                     /note="color: #a6acb3"
     misc_feature    1848..2387
                     /label=IRES
                     /note="color: #a6acb3"
     primer_bind     complement(1992..2009)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="color: black; sequence: GCATTCCTTTGGCGAGAG"
     primer_bind     2219..2238
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     primer_bind     2314..2335
                     /label=IRES2FW
                     /note="color: black; sequence: tacatgtgtttagtcgaggtta; 
                     added: 2023-08-08"
     misc_feature    2375..2399
                     /label=pMIGII_homology_arm
                     /note="color: #a6acb3"
     CDS             2400..2777
                     /codon_start=1
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /product="histone H2B type 1-B"
                     /label=H2bc3
                     /note="histone 1, H2bb; histone cluster 1, H2bb"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS26363.1"
                     /db_xref="GeneID:319178"
                     /db_xref="MGI:MGI:2448377"
                     /protein_id="NP_783595.1"
                     /translation="MPEPSKSAPAPKKGSKKAISKAQKKDGKKRKRSRKESYSVYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     exon            2400..2777
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     gene            2400..2777
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /label=H2bc3
                     /note="H2B clustered histone 3"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:319178"
                     /db_xref="MGI:MGI:2448377"
     misc_feature    2403..2405
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N-acetylproline.
                     /evidence=ECO:0000250|UniProtKB:P23527; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2415..2417
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2433..2435
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2436..2438
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2442..2444
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphoserine, by STK4/MST1. 
                     /evidence=ECO:0000269|PubMed:15197225, 
                     ECO:0000269|PubMed:16039583; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2445..2447
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2448..2450
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2460..2462
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2469..2471
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2481..2771
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Histone H2B; Region: H2B; smart00427"
                     /note="color: #a6acb3"
                     /db_xref="CDD:197718"
     misc_feature    2508..2510
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphoserine, by AMPK. 
                     /evidence=ECO:0000269|PubMed:20647423; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2538..2540
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2571..2573
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6,N6-dimethyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2637..2639
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Dimethylated arginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2655..2657
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2655..2657
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6,N6,N6-trimethyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2658..2660
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2676..2678
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2724..2726
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2736..2738
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="O-linked (GlcNAc) serine. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); glycosylation site"
                     /note="color: #a6acb3"
     misc_feature    2745..2747
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q00729; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2748..2750
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methylated lysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q00729; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     primer_bind     2777..2811
                     /label=HP mC2pMIHP FW
                     /note="color: black; sequence: 
                     ggaCccaccggtcgccaccgGATCCACGGTCGCCA; added: 2022-02-16"
     misc_feature    2820..2858
                     /label=E
                     /note="color: #ff9ccd"
     misc_feature    2859..2876
                     /label=Linker
                     /note="color: #ffef86"
     misc_feature    2877..2900
                     /label=FLAG
                     /note="color: #85dae9"
     misc_feature    2901..2918
                     /label=Linker
                     /note="color: #ffef86"
     tag             2919..2951
                     /label=VSV-g
                     /note="color: #c7b0e3"
     primer_bind     complement(2952..2997)
                     /label=HP mC2pMIHP RV
                     /note="color: black; sequence: 
                     tgcaggtcgactctagagtcgcggccgcTTAcaccatcagcatgcc; added: 
                     2022-02-16"
     misc_feature    2961..2976
                     /label=mcherry
                     /label=nonstandard type:  misc_marker
                     /note="color: #f58a5e"
     misc_feature    2976..2995
                     /label=pMIGII_homology_arm
                     /note="color: #a6acb3"
     LTR             3130..3644
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
                     /note="color: #ffe4c4"
     protein_bind    3806..3822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(3830..3860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 3830 .. 3836 / #ffffff / -10
                        2: 3837 .. 3854 / #ffffff
                        3: 3855 .. 3860 / #ffffff / -35"
     protein_bind    3875..3896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(4013..4030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(4184..4772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #c0c0c0"
     primer_bind     complement(4264..4283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(4943..5803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4943 .. 5734 / #ccffcc
                        2: 5735 .. 5803 / #ccffcc / signal sequence
                     Cleavage site after base 5734"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5566..5585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(5804..5908)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     5976..5994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     primer_bind     complement(6032..6054)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer_bind     6154..6173
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
ORIGIN
        1 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
       61 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
      121 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
      181 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
      241 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
      301 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
      361 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
      421 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
      481 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
      541 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
      601 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
      661 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
      721 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
      781 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
      841 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
      901 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
      961 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     1021 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     1081 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     1141 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     1201 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     1261 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     1321 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     1381 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1441 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1501 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1561 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1621 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1681 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1741 cctcccttta tccagccctc actccttctc taggcgccgg aattccgaac agtcttccgt
     1801 aatagaagac acaaggctcg agcgggatca attccgcccc ccccctaacg ttactggccg
     1861 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1921 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1981 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2041 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2101 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2161 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2221 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2281 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2341 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca
     2401 tgccagagcc ttctaagtct gcaccagccc ctaagaaggg atctaagaaa gccatctcta
     2461 aggcgcagaa gaaggatggc aagaagcgca agcgcagccg caaggagagc tattcggtgt
     2521 acgtgtacaa ggtgctgaag caagtgcacc ccgacaccgg catctcctcc aaggccatgg
     2581 gcatcatgaa ctcgttcgtg aacgacatct tcgagcgcat cgcgagcgag gcgtcccgcc
     2641 tggcgcatta caacaagcgc tcgaccatca cgtcccggga gatccagacg gccgtgcgcc
     2701 tgctgctgcc cggggagctg gccaagcacg ccgtgtcgga gggcactaag gccgtcacca
     2761 agtacaccag ctccaaggac ccaccggtcg ccaccggatc cacggtcgcc accatgggcg
     2821 gggcacctgt gccctatcca gatcccctgg agcctagggg aggaggcggg ggaggggact
     2881 acaaagacga tgacgacaag ggaggaggcg ggggagggta tacagatatc gaaatgaata
     2941 gactcggaaa gggcatgctg atggtgtaag cggccgcgac tctagagtcg acctgcaggc
     3001 atgcaagctt caggtagccg gctaacgtta acaaccggta cctctagaac tatagctagc
     3061 atgcgcaaat ttaaagcgct gatatcgata aaataaaaga ttttatttag tctccagaaa
     3121 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     3181 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     3241 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     3301 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     3361 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     3421 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     3481 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
     3541 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     3601 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa cagtttcttg
     3661 aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct gactcaatta
     3721 gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat ggacagcgca
     3781 gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac aacatacgag
     3841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5941 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     6001 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     6061 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6121 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     6181 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6241 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6301 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6361 gg
//
