LOCUS       Exported                6383 bp ds-DNA     circular SYN 25-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Orian Bricard
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     5..27
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     155..174
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
                     /note="color: black; sequence: GGTGATGTCGGCGATATAGG"
     LTR             374..890
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
                     /note="color: #ffe4c4"
     misc_feature    954..1295
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
                     /note="color: #ffe4c4"
     CDS             1362..1778
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1708..1730
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
                     /note="color: black; sequence: CCCTTGAACCTCCTCGTTCGACC"
     primer_bind     1714..1729
                     /label=MGFW
                     /note="color: black; sequence: aacctcctcgttcgac; added: 
                     2023-08-08"
     primer_bind     1746..1762
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
                     /note="color: black; sequence: CTTTATCCAGCCCTCAC"
     misc_feature    1848..2387
                     /label=IRES
                     /note="color: #a6acb3"
     primer_bind     complement(1992..2009)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="color: black; sequence: GCATTCCTTTGGCGAGAG"
     primer_bind     2219..2238
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     primer_bind     2314..2335
                     /label=IRES2FW
                     /note="color: black; sequence: tacatgtgtttagtcgaggtta; 
                     added: 2023-08-08"
     misc_feature    2375..2399
                     /label=pMIGII_homology_arm
                     /note="color: #a6acb3"
     CDS             2400..2777
                     /codon_start=1
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /product="histone H2B type 1-B"
                     /label=H2bc3
                     /note="histone 1, H2bb; histone cluster 1, H2bb"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS26363.1"
                     /db_xref="GeneID:319178"
                     /db_xref="MGI:MGI:2448377"
                     /protein_id="NP_783595.1"
                     /translation="MPEPSKSAPAPKKGSKKAISKAQKKDGKKRKRSRKESYSVYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     exon            2400..2777
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     gene            2400..2777
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /label=H2bc3
                     /note="H2B clustered histone 3"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:319178"
                     /db_xref="MGI:MGI:2448377"
     misc_feature    2403..2405
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N-acetylproline.
                     /evidence=ECO:0000250|UniProtKB:P23527; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2415..2417
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2433..2435
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2436..2438
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2442..2444
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphoserine, by STK4/MST1. 
                     /evidence=ECO:0000269|PubMed:15197225, 
                     ECO:0000269|PubMed:16039583; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2445..2447
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2448..2450
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2460..2462
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2469..2471
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P33778; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2481..2771
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Histone H2B; Region: H2B; smart00427"
                     /note="color: #a6acb3"
                     /db_xref="CDD:197718"
     misc_feature    2508..2510
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphoserine, by AMPK. 
                     /evidence=ECO:0000269|PubMed:20647423; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2538..2540
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2571..2573
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6,N6-dimethyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2637..2639
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Dimethylated arginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2655..2657
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2655..2657
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6,N6,N6-trimethyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2658..2660
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2676..2678
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0000250|UniProtKB:Q96A08; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2724..2726
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2736..2738
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="O-linked (GlcNAc) serine. 
                     /evidence=ECO:0000250|UniProtKB:P62807; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); glycosylation site"
                     /note="color: #a6acb3"
     misc_feature    2745..2747
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q00729; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2748..2750
                     /gene="H2bc3"
                     /gene_synonym="H2b-143; Hist1h2; Hist1h2bb"
                     /note="N6-methylated lysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q00729; propagated from 
                     UniProtKB/Swiss-Prot (Q64475.3); methylation site"
                     /note="color: #a6acb3"
     primer_bind     2777..2811
                     /label=HP mC2pMIHP FW
                     /note="color: black; sequence: 
                     ggaCccaccggtcgccaccgGATCCACGGTCGCCA; added: 2022-02-16"
     misc_feature    2820..2876
                     /label=HAT tag
                     /note="color: #b1ff67"
     misc_feature    2877..2894
                     /label=Linker
                     /note="color: #ffef86"
     misc_feature    2895..2921
                     /label=NWS
                     /label=Strep II
                     /note="color: #85dae9"
     misc_feature    2922..2939
                     /label=Linker
                     /note="color: #ffef86"
     tag             2940..2972
                     /label=VSV-g
                     /note="color: #85dae9"
     primer_bind     complement(2973..3018)
                     /label=HP mC2pMIHP RV
                     /note="color: black; sequence: 
                     tgcaggtcgactctagagtcgcggccgcTTAcaccatcagcatgcc; added: 
                     2022-02-16"
     misc_feature    2982..2997
                     /label=mcherry
                     /label=nonstandard type:  misc_marker
                     /note="color: #f58a5e"
     misc_feature    2997..3016
                     /label=pMIGII_homology_arm
                     /note="color: #a6acb3"
     LTR             3151..3665
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
                     /note="color: #ffe4c4"
     protein_bind    3827..3843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(3851..3881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 3851 .. 3857 / #ffffff / -10
                        2: 3858 .. 3875 / #ffffff
                        3: 3876 .. 3881 / #ffffff / -35"
     protein_bind    3896..3917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(4034..4051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(4205..4793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #c0c0c0"
     primer_bind     complement(4285..4304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(4964..5824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4964 .. 5755 / #ccffcc
                        2: 5756 .. 5824 / #ccffcc / signal sequence
                     Cleavage site after base 5755"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5587..5606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(5825..5929)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     5997..6015
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     primer_bind     complement(6053..6075)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer_bind     6175..6194
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
ORIGIN
        1 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
       61 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
      121 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
      181 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
      241 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
      301 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
      361 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
      421 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
      481 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
      541 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
      601 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
      661 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
      721 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
      781 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
      841 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
      901 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
      961 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     1021 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     1081 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     1141 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     1201 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     1261 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     1321 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     1381 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1441 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1501 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1561 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1621 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1681 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1741 cctcccttta tccagccctc actccttctc taggcgccgg aattccgaac agtcttccgt
     1801 aatagaagac acaaggctcg agcgggatca attccgcccc ccccctaacg ttactggccg
     1861 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1921 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1981 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2041 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2101 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2161 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2221 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2281 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2341 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca
     2401 tgccagagcc ttctaagtct gcaccagccc ctaagaaggg atctaagaaa gccatctcta
     2461 aggcgcagaa gaaggatggc aagaagcgca agcgcagccg caaggagagc tattcggtgt
     2521 acgtgtacaa ggtgctgaag caagtgcacc ccgacaccgg catctcctcc aaggccatgg
     2581 gcatcatgaa ctcgttcgtg aacgacatct tcgagcgcat cgcgagcgag gcgtcccgcc
     2641 tggcgcatta caacaagcgc tcgaccatca cgtcccggga gatccagacg gccgtgcgcc
     2701 tgctgctgcc cggggagctg gccaagcacg ccgtgtcgga gggcactaag gccgtcacca
     2761 agtacaccag ctccaaggac ccaccggtcg ccaccggatc cacggtcgcc accatgggca
     2821 aggaccacct gatccacaac gtgcacaagg agttccacgc ccacgcccac aacaagggag
     2881 gaggcggggg agggaactgg tcccaccccc aattcgagaa aggaggaggc gggggagggt
     2941 atacagatat cgaaatgaat agactcggaa agggcatgct gatggtgtaa gcggccgcga
     3001 ctctagagtc gacctgcagg catgcaagct tcaggtagcc ggctaacgtt aacaaccggt
     3061 acctctagaa ctatagctag catgcgcaaa tttaaagcgc tgatatcgat aaaataaaag
     3121 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     3181 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     3241 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     3301 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     3361 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     3421 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     3481 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     3541 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     3601 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     3661 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     3721 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     3781 tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     3841 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3901 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4201 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4261 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4321 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4381 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4441 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4501 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4561 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4621 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4681 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4801 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6001 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6061 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     6241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     6301 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     6361 gattaagttg ggtaacgcca ggg
//
