LOCUS       UAS_dL5_mCer3           6063 bp    DNA        circular     20-JUN-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2365..3084
                     /locus_tag="mCer"
                     /label="mCer"
                     /ApEinfo_label="mCer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1564..2328
                     /locus_tag="dL5"
                     /label="dL5"
                     /ApEinfo_label="dL5"
                     /ApEinfo_fwdcolor="#b86c08"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             4460..5119
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             3755..3823
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_binding    176..198
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      5217..5899
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(3667..3684)
                     /PCR_conditions="primer sequence:TGTAAAACGACGGCCAGT"
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     204..224
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     polyA_site      3100..3520
                     /locus_tag="Ocean pout afp polyA"
                     /label="Ocean pout afp polyA"
                     /ApEinfo_label="Ocean pout afp polyA"
                     /ApEinfo_fwdcolor="#ffc0cb"
                     /ApEinfo_revcolor="#ffc0cb"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    425..849
                     /locus_tag="14xUAS-E1b"
                     /label="14xUAS-E1b"
                     /ApEinfo_label="14xUAS-E1b"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#804000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    826..849
                     /locus_tag="Carp b-actin xcrn +24"
                     /label="Carp b-actin xcrn +24"
                     /ApEinfo_label="Carp b-actin xcrn +24"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    785..825
                     /locus_tag="E1b basal promoter"
                     /label="E1b basal promoter"
                     /ApEinfo_label="E1b basal promoter"
                     /ApEinfo_fwdcolor="#804040"
                     /ApEinfo_revcolor="#804040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    255..413
                     /locus_tag="tol1-left"
                     /label="tol1-left"
                     /ApEinfo_label="tol1-left"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3546..3651
                     /locus_tag="tol1-right"
                     /label="tol1-right"
                     /ApEinfo_label="tol1-right"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    869..1557
                     /locus_tag="UBC intron (+1a)"
                     /label="UBC intron (+1a)"
                     /ApEinfo_label="UBC intron (+1a)"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgGTCCCTT TAGCCAGTAG CGGTTCTAGG CACGGGCCGT CCGGGCGGTG GCCTGGGGCG
      301 GAAAACTGAA GGGGGGCGGC ACCGGCGGCT CAGCCCTTGT AATATATTAT ATGCACCACT
      361 ATTGGTTTAC TTATGTCACA GTTTGTAAGT TTGTAACAGC CTGAACCTGG ATCAGCTTAG
      421 GCCTCCAAGG CGGAGTACTG TCCTCCGGGC TGGCGGAGTA CTGTCCTCCG GCAAGGTCGG
      481 AGTACTGTCC TCCGACACTA GAGGTCGGAG TACTGTCCTC CGACGCAAGG CGGAGTACTG
      541 TCCTCCGGGC TGCGGAGTAC TGTCCTCCGG CAAGGTCGGA GTACTGTCCT CCGACACTAG
      601 AGGTCGGAGT ACTGTCCTCC GACGCAAGGT CGGAGTACTG TCCTCCGACA CTAGAGGTCG
      661 GAGTACTGTC CTCCGACGCA AGGTCGGAGT ACTGTCCTCC GACACTAGAG GTCGGAGTAC
      721 TGTCCTCCGA CGCAAGGCGG AGTACTGTCC TCCGGGCTGG CGGAGTACTG TCCTCCGGCA
      781 AGGGTCGACT CTAGAGGGTA TATAATGGAT CCCATCGCGT CTCAGCCTCA CTTTGAGCTC
      841 CTCCACACGa attcgatact aaggatccTT GTTTgtgagt atgatgttaa atataagcaa
      901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa
      961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg
     1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa
     1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc
     1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct
     1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt
     1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt
     1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt
     1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc
     1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca
     1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gCGTAAAgcc
     1561 acCATGGAAT TCggcctttc tggcctgCAG GCCGTCGTTA CCCAAGAACC TAGTGTTACC
     1621 GTTAGCCCAG GTGGTACTGT TATACTTACT TGTGGAAGTG GTACGGGTGC CGTCACATCT
     1681 GGTCATTATG CAAATTGGTT TCAACAAAAA CCAGGACAAG CTCCAAGAGC TTTGATTTTT
     1741 GATACTGATA AGAAGTATTC TTGGACCCCA GGTAGATTTT CTGGATCTTT GCTGGGAGCA
     1801 AAGGCAGCTT TGACAATATC AGATGCTCAG CCTGAGGACG AAGCCGAGTA TTACTGTTCT
     1861 CTTAGCGACG TGGATGGCTA CTTGTTTGGC GGTGGAACAC AACTGACGGT TCTGTCCGGT
     1921 GGTGGCGGCT CTGGTGGCGG TGGCAGCGGC GGTGGTGGTT CCGGAGGCGG CGGTTCTCAG
     1981 GCTGTGGTGA CTCAGGAGCC GTCAGTGACT GTGTCCCCAG GAGGGACAGT CATTCTCACT
     2041 TGTGGCTCCG GCACTGGAGC TGTCACCAGT GGTCATTATG CCAACTGGTT CCAGCAGAAG
     2101 CCTGGCCAAG CCCCCAGGGC ACTTATATTT GACACCGACA AGAAGTATTC CTGGACCCCT
     2161 GGCCGATTCT CAGGCTCCCT CCTTGGGGCC AAGGCTGCCC TGACCATCTC GGATGCGCAG
     2221 CCTGAAGATG AgGCTGAGTA TTACTGTTCG CTCTCCGACG TTGACGGTTA TCTGTTCGGA
     2281 GGAGGCACCC AGCTGACCGT CCTCTCCgcg gccggtctgg ccCATATGgg aggcggtggg
     2341 tctggtggcg gtggcagcAC CGGTATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
     2401 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
     2461 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
     2521 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA GCTGGGGCGT GCAGTGCTTC
     2581 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
     2641 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
     2701 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
     2761 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACG CCATCCACGG CAACGTCTAT
     2821 ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT TCGGCCTCAA CTGCAACATC
     2881 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
     2941 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC
     3001 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
     3061 GGCATGGACG AGCTGTACAA GTAACTCGAG CCTctagtaa ttaagtctca gccaccgtta
     3121 actgaacatg tcaaaacctg tggagactgt tgagatttga tgttctgaaa agataaagcc
     3181 tataaataaa atgttgccca aatttcctgc ctgatgtttt tctttgtctt tgctacatgg
     3241 ctttgctgct cggatcggct cactctgtgt atgccacgtt cactttgtac tctccttctc
     3301 acggtaggtt tattattttt agatgtgcag ttagtttctg tgaaataaca caccacacac
     3361 tgatattgtc tgtgcattga cttggtgagt gcacattgtt tttgatcttg acatatttat
     3421 atttgattga tcaggtgaac tgtgtgaatc taaagtgctc catacagatg ttctgcattg
     3481 aaaatattct cattttatta gtggaagtga gtgtatgcca gcggccgcgg tacCCTGCAG
     3541 TCTAGACATT TCAGTTGACG AAGACAAACA AAGTTCTGTT GTGACTATGG GGGGGGGGGG
     3601 CGCCTGGGGA TGGTCTCGCC CGGGGAGTAA TTCAGGGTAG AACCGCCACT GCCTTTAGCG
     3661 ACattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3721 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     3841 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     3901 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3961 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4021 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4081 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4261 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4321 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4381 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4441 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4501 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4561 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4621 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4681 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4741 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4801 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4861 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4921 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4981 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5041 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5101 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5701 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5941 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6001 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6061 aga     
//
