LOCUS       pCAGGS-CIBN-p53CT(98-39 6866 bp ds-DNA     circular     24-NOV-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    83..665
                     /label="CAG promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    88..375
                     /label="CAG enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1653..1677
                     /label="CAG1640"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1688..1709
                     /label="cag1700-seq-fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1731..1731
                     /label="MCS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1740..2252
                     /label="CIBN"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1740..2253
                     /label="CIBN"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1740..3878
                     /label="Translation 1740-3878"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSSMITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCNEAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKELEKTDYIHLEAMPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSDGGRMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKSR*"
     misc_feature    2265..2849
                     /label="DNA-binding domain"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2871..2942
                     /label="NLS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2943..3038
                     /label="Tetramerization domain"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3060..3152
                     /label="Basic domain"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3165..3869
                     /label="mNG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3871..3884
                     /label="MCS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3906..3931)
                     /label="caggs-seq-rev"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3918..3941)
                     /label="CAGGS-R2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(4365..4385)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(4391..4413)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(4417..4447)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     nucleotide      4542..4872
                     /label="SV40 Ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(5098..5780)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     protein         complement(5878..6735)
                     /label="Amp_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cgggggcgag
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1681 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gAATTCACCA
     1741 TGAATGGAGC TATAGGAGGT GACCTTTTGC TCAATTTTCC TGACATGTCG GTCCTAGAGC
     1801 GCCAAAGGGC TCACCTCAAG TACCTCAATC CCACCTTTGA TTCTCCTCTC GCCGGCTTCT
     1861 TTGCCGATTC TTCAATGATT ACCGGCGGCG AGATGGACAG CTATCTTTCG ACTGCCGGTT
     1921 TGAATCTTCC GATGATGTAC GGTGAGACGA CGGTGGAAGG TGATTCAAGA CTCTCAATTT
     1981 CGCCGGAAAC GACGCTTGGG ACTGGAAATT TCAAGGCAGC GAAGTTTGAT ACAGAGACTA
     2041 AGGATTGTAA TGAGGCGGCG AAGAAGATGA CGATGAACAG AGATGACCTA GTAGAAGAAG
     2101 GAGAAGAAGA GAAGTCGAAA ATAACAGAGC AAAACAATGG GAGCACAAAA AGCATCAAGA
     2161 AGATGAAACA CAAAGCCAAG AAAGAAGAGA ACAATTTCTC TAATGATTCA TCTAAAGTGA
     2221 CGAAGGAATT GGAGAAAACG GATTATATTC ATCtcgaggc cATGCCTTCC CAGAAAACCT
     2281 ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC AAGTCTGTGA
     2341 CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG ACCTGCCCTG
     2401 TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC ATGGCCATCT
     2461 ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT GAGCGCTGCT
     2521 CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA AATTTGCGTG
     2581 TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC TATGAGCCGC
     2641 CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC AGTTCCTGCA
     2701 TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC TCCAGTGGTA
     2761 ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG AGAGACCGGC
     2821 GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG CCCCCAGGGA
     2881 GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG AAGAAACCAC
     2941 TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG ATGTTCCGAG
     3001 AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA GGGGGGAGCA
     3061 GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC CATAAAAAAC
     3121 TCATGTTCAA GACAGAAGGG CCTGActcag acggcGGCCG CATGGTGAGC AAGGGCGAGG
     3181 AGGATAACAT GGCCTCTCTC CCAGCGACAC ATGAGTTACA CATCTTTGGC TCCATCAACG
     3241 GTGTGGACTT TGACATGGTG GGTCAGGGCA CCGGCAATCC AAATGATGGT TATGAGGAGT
     3301 TAAACCTGAA GTCCACCAAG GGTGACCTCC AGTTCTCCCC CTGGATTCTG GTCCCTCATA
     3361 TCGGGTATGG CTTCCATCAG TACCTGCCCT ACCCTGACGG GATGTCGCCT TTCCAGGCCG
     3421 CCATGGTAGA TGGCTCCGGC TACCAAGTCC ATCGCACAAT GCAGTTTGAA GATGGTGCCT
     3481 CCCTTACTGT TAACTACCGC TACACCTACG AGGGAAGCCA CATCAAAGGA GAGGCCCAGG
     3541 TGAAGGGGAC TGGTTTCCCT GCTGACGGTC CTGTGATGAC CAACTCGCTG ACCGCTGCGG
     3601 ACTGGTGCAG GTCGAAGAAG ACTTACCCCA ACGACAAAAC CATCATCAGT ACCTTTAAGT
     3661 GGAGTTACAC CACTGGAAAT GGCAAGCGCT ACCGGAGCAC TGCGCGGACC ACCTACACCT
     3721 TTGCCAAGCC AATGGCGGCT AACTATCTGA AGAACCAGCC GATGTACGTG TTCCGTAAGA
     3781 CGGAGCTCAA GCACTCCAAG ACCGAGCTCA ACTTCAAGGA GTGGCAAAAG GCCTTTACCG
     3841 ATGTGATGGG CATGGACGAG CTGTACAAGT ctagaTAAgt cgacggatct ttttccctct
     3901 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3961 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     4021 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     4081 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     4141 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     4201 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     4261 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     4321 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     4381 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4441 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4501 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     4561 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4621 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4681 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4741 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4861 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5281 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     5341 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5521 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5641 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6841 cacatttccc cgaaaagtgc cacctg
//