LOCUS       pPB-p53RE(CDKN1A)-CMVmi 7189 bp ds-DNA     circular     15-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(135..441)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(460..528)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     599..616
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     625..645
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    670..983
                     /label="5'PB terminal repeat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1009..1025
                     /label="CAG promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    complement(1031..1222)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein         complement(1232..1828)
                     /label="Puro_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(1848..2240)
                     /label="PGK promoter (pSiRPG)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        complement(1852..2250)
                     /label="PGKp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     Insulator       complement(2251..2327)
                     /label="Insulator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2334..3348
                     /label="p53RE(CDKN1A)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2397..2416
                     /label="p53BS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3280..3299
                     /label="p53BS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3361..3485
                     /label="CMVmin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3384..3390
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3497..4192
                     /label="mScarlet-I"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3497..4264
                     /label="Translation 3497-4264"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYKPKKKRKVDPKKKRKVDPKKKRKV*"
     misc_feature    4193..4261
                     /label="NLSx3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4271..4291
                     /label="AU1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4298..4522
                     /label="BGH pA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(4546..4566)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(4572..4594)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(4598..4628)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4723..4964
                     /label="3' PB terminal repeat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(4999..5018)
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(5001..5019)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(5036..5056)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(5062..5084)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(5088..5118)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(5424..6106)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(6204..6863)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA
     1081 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
     1141 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
     1201 TCAATGTATC TTATCATGTC TGggatccct aGGCACCGGG CTTGCGGGTC ATGCACCAGG
     1261 TGCGCGGTCC TTCGGGCACC TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA
     1321 AGGGGAGGTT GCGGGGCGCG GAGGTCTCCA GGAAGGCGGG CACCCCGGCG CGCTCGGCCG
     1381 CCTCCACTCC GGGGAGCACG ACGGCGCTGC CCAGACCCTT GCCCTGGTGG TCGGGCGAGA
     1441 CGCCGACGGT GGCCAGGAAC CACGCGGGCT CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT
     1501 CCATCTGTTG CTGCGCGGCC AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT
     1561 CGGCGAACAC CGCCCCCGCT TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC
     1621 CGTCGTCCGC GACCCACACC TTGCCGATGT CGAGCCCGAC GCGCGTGAGG AAGAGTTCTT
     1681 GCAGCTCGGT GACCCGCTCG ATGTGGCGGT CCGGATCGAC GGTGTGGCGC GTGGCGGGGT
     1741 AGTCGGCGAA CGCGGCGGCG AGGGTGCGTA CGGCCCTGGG GACGTCGTCG CGGGTGGCGA
     1801 GGCGCACCGT GGGCTTGTAC TCGGTCATgg atccggtaag ctagcttggg gtcgaaaggc
     1861 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
     1921 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
     1981 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
     2041 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
     2101 gtctagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
     2161 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
     2221 gagccggttg gcgcctaccg gtggatgtgg CCCCCCCAAA GCCCCCAGGG ATGTAATTAC
     2281 GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG CGGCGCGgaa ttcCCTGCTT
     2341 CCCAGGAACA TGCTTGGGCA GCAGGCTGTG GCTCTGATTG GCTTTCTGGC CGTCAGGAAC
     2401 ATGTCCCAAC ATGTTGAGCT CTGGCATAGA AGAGGCTGGT GGCTATTTTG TCCTTGGGCT
     2461 GCCTGTTTTC AGGTGAGGAA GGGGATGGTA GGAGACAGGA GACCTCTAAA GACCCCAGGT
     2521 AAACCTTAGC CTGTTACTCT GAACAGGGTA TGTGATCTGC CAGCAGATCC TTGCGACAGG
     2581 GCTGGGATCT GATGCATGTG TGCTTGTGTG AGTGTGTGCT GGGAGTCAGA TTCTGTGTGT
     2641 GACTTTTAAC AGCCTGCTCC CTTGCCTTCT TCAGGGCAGA AGTCCTCCCT TAGAGTGTGT
     2701 CTGGGTACAC ATTCAAGTGC ATGGTTGCAA ACTTTTTTTT TTTAAAGCAC TGAATAGTAC
     2761 TAGACACTTA GTAGGTACTT AAGAAATATT GAATGTCGTG GTGGTGGTGA GCTAGAAGTT
     2821 ATAAAAAAAA TTCTTTCCCA AAAACAACAA CAAAAAGAAT TATTTCATTG TGAAGCTCAG
     2881 TACCACAAAA ATTTAAATAA TTCATTACAA GCCTTTATTA AAAAAAATTT TCTCCCCAAA
     2941 GTAAACAGAC AGACAATGTC TAGTCTATTT GAAATGCCTG AAAGCAGAGG GGCTTCAAGG
     3001 CAGTGGGAGA AGGTGCCTGT CCTCTGCTGG ACATTTGACA ACCAGCCCTT TGGATGGTTT
     3061 GGATGTATAG GAGCGAAGGT GCAGACAGCA GTGGGGCTTA GAGTGGGGTC CTGAGGCTGT
     3121 GCCGTGGCCT TTCTGGGGTT TAGCCACAAT CCTGGCCTGA CTCCAGGGCG AGGCAGGCCA
     3181 AGGGGGTCTG CTACTGTGTC CTCCCACCCC TACCTGGGCT CCCATCCCCA CAGCAGAGGA
     3241 GAAAGAAGCC TGTCCTCCCC GAGGTCAGCT GCGTTAGAGG AAGAAGACTG GGCATGTCTG
     3301 GGCAGAGATT TCCAGACTCT GAGCAGCCTG AGATGTCAGT AATTGTAGct cgagTACGTA
     3361 GTAGGCGTGT ACGGTGGGAG GCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG
     3421 CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC
     3481 TCCGCgcggc cgcgccATGG TGAGCAAGGG CGAGGCAGTG ATCAAGGAGT TCATGCGGTT
     3541 CAAGGTGCAC ATGGAGGGCT CCATGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA
     3601 GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT
     3661 GCCCTTCTCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAGGG CCTTCATCAA
     3721 GCACCCCGCC GACATCCCCG ACTACTATAA GCAGTCCTTC CCCGAGGGCT TCAAGTGGGA
     3781 GCGCGTGATG AACTTCGAGG ACGGCGGCGC CGTGACCGTG ACCCAGGACA CCTCCCTGGA
     3841 GGACGGCACC CTGATCTACA AGGTGAAGCT CCGCGGCACC AACTTCCCTC CTGACGGCCC
     3901 CGTAATGCAG AAGAAGACAA TGGGCTGGGA AGCGTCCACC GAGCGGTTGT ACCCCGAGGA
     3961 CGGCGTGCTG AAGGGCGACA TTAAGATGGC CCTGCGCCTG AAGGACGGAG GCCGCTACCT
     4021 GGCGGACTTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGATGCCCG GCGCCTACAA
     4081 CGTTGACCGC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCGTGG TGGAACAGTA
     4141 CGAACGCTCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGCCAAAGAA
     4201 AAAGAGAAAA GTAGATCCAA AAAAGAAGAG AAAGGTAGAT CCAAAAAAGA AGAGAAAGGT
     4261 Ataggtcgac ATTTATTTAT TTATTTATTT Aagatctctg tgccttctag ttgccagcca
     4321 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4381 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4441 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4501 ggggatgcgg tgggctctat ggctgcagcc caagcttggc gtaatcatgg tcatagctgt
     4561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4681 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctTAAAAGTT TTGTTACTTT
     4741 ATAGAAGAAA TTTTGAGTTT TTGTTTTTTT TTAATAAATA AATAAACATA AATAAATTGT
     4801 TTGTTGAATT TATTATTAGT ATGTAAGTGT AAATATAATA AAACTTAATA TCTATTCAAA
     4861 TTAATAAATA AACCTCGATA TACAGACCGA TAAAACACAT GCGTCAATTT TACGCATGAT
     4921 TATCTTTAAC GTACGTCACA ATATGATTAT CTTTCTAGGG TTAATCTAGT ATACGCGTtt
     4981 aattaatcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     5041 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5101 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5161 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5221 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5881 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6481 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//