LOCUS       RARe-RFP-PGK-H2BGFP     8081 bp ds-DNA     circular     06-MAR-2024
DEFINITION  .
COMMENT     Nikolova_S7C_2_RARe_E3
FEATURES             Location/Qualifiers
     promoter        871..901
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_binding    905..927
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     933..953
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        970..988
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     971..990
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1470..1595
                     /label="HIV-1 psi"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2088..2321
                     /label="RRE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2727..2782
                     /label="RARe"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2799..2995
                     /label="SV40"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3012..3683
                     /label="mRFP"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3733..3850
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3899..4409
                     /label="PGK"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4421..4798
                     /label="Histone H2B"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4817..5536
                     /label="EGFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(6236..6256)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(6265..6282)
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             6353..6421
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      6440..6746
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             7207..7866
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
       61 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
      121 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
      181 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
      241 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
      301 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
      361 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
      421 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
      481 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
      541 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
      601 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
      661 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
      721 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG
      781 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT
      841 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT
      901 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC
      961 CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTGCAAG CTTAATGTAG
     1021 TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA
     1081 CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC
     1141 TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT
     1201 TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACATAA ACGGGTCTCT CTGGTTAGAC
     1261 CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA
     1321 AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG
     1381 AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG
     1441 ACTTGAAAGC GAAAGGGAAA CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG
     1501 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG
     1561 GAGGCTAGAA GGAGAGAGAT GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT
     1621 CGCGATGGGA AAAAATTCGG TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA
     1681 TAGTATGGGC AAGCAGGGAG CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT
     1741 CAGAAGGCTG TAGACAAATA CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG
     1801 AACTTAGATC ATTATATAAT ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA
     1861 TAAAAGACAC CAAGGAAGCT TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA
     1921 CCGCACAGCA AGCGGCCGCT GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG
     1981 AGAAGTGAAT TATATAAATA TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC
     2041 AAGGCAAAGA GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC
     2101 CTTGGGTTCT TGGGAGCAGC AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA
     2161 CAGGCCAGAC AATTATTGTC TGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT
     2221 GAGGCGCAAC AGCATCTGTT GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA
     2281 ATCCTGGCTG TGGAAAGATA CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT
     2341 GGAAAACTCA TTTGCACCAC TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG
     2401 GAACAGATTT GGAATCACAC GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA
     2461 AGCTTAATAC ACTCCTTAAT TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA
     2521 TTATTGGAAT TAGATAAATG GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG
     2581 TGGTATATAA AATTATTCAT AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT
     2641 GCTGTACTTT CTATAGTGAA TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC
     2701 CACCTCCCAA CCCCGAGGGG ACTCGACAGT TGGGTCATTT GAAGGTTAGC AGCCCGGGTA
     2761 GGGTTCACCG AAAGTTCACT CGCTCGAGAT CTGCGATCTG CATCTCAATT AGTCAGCAAC
     2821 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
     2881 TCCGCCCCAT CGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC
     2941 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
     3001 TGCTAGCCAT GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA
     3061 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
     3121 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT
     3181 GGGACATCCT GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
     3241 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
     3301 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
     3361 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
     3421 AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
     3481 AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCCGAGGTCA
     3541 AGACCACCTA CATGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAG ACCGACATCA
     3601 AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAGCGCGCCG
     3661 AGGGCCGCCA CTCCACCGGC GCCTAAGAAT GCAATTCTCG ACCTCGAGAC AAATGGCAGT
     3721 ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT
     3781 AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAAAT
     3841 TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA CTTTGGCCGC GGCTCGAGGG
     3901 GGTTGGGGTT GCGCCTTTTC CAAGGCAGCC CTGGGTTTGC GCAGGGACGC GGCTGCTCTG
     3961 GGCGTGGTTC CGGGAAACGC AGCGGCGCCG ACCCTGGGTC TCGCACATTC TTCACGTCCG
     4021 TTCGCAGCGT CACCCGGATC TTCGCCGCTA CCCTTGTGGG CCCCCCGGCG ACGCTTCCTG
     4081 CTCCGCCCCT AAGTCGGGAA GGTTCCTTGC GGTTCGCGGC GTGCCGGACG TGACAAACGG
     4141 AAGCCGCACG TCTCACTAGT ACCCTCGCAG ACGGACAGCG CCAGGGAGCA ATGGCAGCGC
     4201 GCCGACCGCG ATGGGCTGTG GCCAATAGCG GCTGCTCAGC AGGGCGCGCC GAGAGCAGCG
     4261 GCCGGGAAGG GGCGGTGCGG GAGGCGGGGT GTGGGGCGGT AGTGTGGGCC CTGTTCCTGC
     4321 CCGCGCGGTG TTCCGCATTC TGCAAGCCTC CGGAGCGCAC GTCGGCAGTC GGCTCCCTCG
     4381 TTGACCGAAT CACCGACCTC TCTCCCCAGG GGGATCTACC ATGCCAGAGC CAGCGAAGTC
     4441 TGCTCCCGCC CCGAAAAAGG GCTCCAAGAA GGCGGTGACT AAGGCGCAGA AGAAAGGCGG
     4501 CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCATC TATGTGTACA AGGTTCTGAA
     4561 GCAGGTCCAC CCTGACACCG GCATTTCGTC CAAGGCCATG GGCATCATGA ACTCGTTTGT
     4621 GAACGACATT TTCGAGCGCA TCGCAGGTGA GGCTTCCCGC CTGGCGCATT ACAACAAGCG
     4681 CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC CTGCTGCTGC CTGGGGAGTT
     4741 GGCCAAGCAC GCCGTGTCCG AGGGTACTAA GGCCATCACC AAGTACACCA GCGCTAAGGA
     4801 TCCACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT
     4861 CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA
     4921 GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC
     4981 CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA
     5041 CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA
     5101 GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT
     5161 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG
     5221 CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC
     5281 CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG
     5341 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
     5401 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
     5461 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
     5521 CGAGCTGTAC AAGTAAATGC ATCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA
     5581 GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA
     5641 AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
     5701 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
     5761 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
     5821 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT
     5881 TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT
     5941 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
     6001 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA
     6061 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC
     6121 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC
     6181 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG GACGTACCCA ATTCGCCCTA
     6241 TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     6301 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     6361 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     6421 GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC
     6481 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC
     6541 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT
     6601 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG
     6661 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG
     6721 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT
     6781 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT
     6841 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT
     6901 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
     6961 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
     7021 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
     7081 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
     7141 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
     7201 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
     7261 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
     7321 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
     7381 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
     7441 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
     7501 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
     7561 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
     7621 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
     7681 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
     7741 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
     7801 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
     7861 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT
     7921 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT
     7981 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
     8041 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA C
//