LOCUS       Empty(Ctrl)-eGFP-PGK-H2 8473 bp ds-DNA     circular     24-MAR-2024
DEFINITION  .
COMMENT     Racelis_n8j_1_MattT-1
FEATURES             Location/Qualifiers
     CDS             complement(1586..2257)
                     /label="mRFP"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(5546..5565)
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(5548..5566)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(5583..5603)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(5609..5631)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(5635..5665)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(5971..6653)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(6751..7410)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(7871..8177)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(8196..8264)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     8335..8352
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     8361..8381
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG
       61 GATGGGCGGA GTTAGGGGCG GGATAGCTAG AGCCAGACAT GATAAGATAC ATTGATGAGT
      121 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
      181 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTCCTCTC ACTCTCTGAT
      241 ATTCATTTCT TTGCAAGTTA TAAATACTGA ATAATAAGAT GACATGAACT ACTACTGCTA
      301 GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC
      361 AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG
      421 TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATTTGGT CTAACCAGAG AGACCCAGTA
      481 CAAGCAAAAA GCAGATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT
      541 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGCATC
      601 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
      661 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
      721 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
      781 GCTTTTGCAA AAAGCTGGGG GATCCACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC
      841 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT
      901 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA
      961 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG
     1021 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
     1081 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
     1141 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
     1201 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
     1261 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA
     1321 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
     1381 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC
     1441 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG
     1501 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGCGAC TCTAGAGGAT
     1561 CCTCACGTCG ACTAGTCTCG AGTTAGGCGC CGGTGGAGTG GCGGCCCTCG GCGCGCTCGT
     1621 ACTGTTCCAC GATGGTGTAG TCCTCGTTGT GGGAGGTGAT GTCCAGCTTG ATGTCGGTCT
     1681 TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT TGGCCATGTA GGTGGTCTTG ACCTCGGCGT
     1741 CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC TCATCTTGAT CTCGCCCTTC AGGGCGCCGT
     1801 CCTCGGGGTA CATCCGCTCG GTGGAGGCCT CCCAGCCCAT GGTCTTCTTC TGCATTACGG
     1861 GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA GCTTCACCTT GTAGATGAAC TCGCCGTCCT
     1921 GCAGGGAGGA GTCCTGGGTC ACGGTCACCA CGCCGCCGTC CTCGAAGTTC ATCACGCGCT
     1981 CCCACTTGAA GCCCTCGGGG AAGGACAGCT TCAAGTAGTC GGGGATGTCG GCGGGGTGCT
     2041 TCACGTAGGC CTTGGAGCCG TACTGGAACT GAGGGGACAG GATGTCCCAG GCGAAGGGCA
     2101 GGGGGCCGCC CTTGGTCACC TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT
     2161 CGCCCTCGCC CTCGATCTCG AACTCGTGGC CGTTCACGGA GCCCTCCATG CGCACCTTGA
     2221 AGCGCATGAA CTCCTTGATG ACGTCCTCGG AGGAGGCCAT GGTGGCGACC GGTGGATCCT
     2281 TAGCGCTGGT GTACTTGGTG ATGGCCTTAG TACCCTCGGA CACGGCGTGC TTGGCCAACT
     2341 CCCCAGGCAG CAGCAGGCGC ACGGCCGTCT GGATCTCCCT GGAGGTGATG GTCGAGCGCT
     2401 TGTTGTAATG CGCCAGGCGG GAAGCCTCAC CTGCGATGCG CTCGAAAATG TCGTTCACAA
     2461 ACGAGTTCAT GATGCCCATG GCCTTGGACG AAATGCCGGT GTCAGGGTGG ACCTGCTTCA
     2521 GAACCTTGTA CACATAGATG GAATAGCTCT CCTTGCGGCT GCGCTTGCGC TTCTTGCCGC
     2581 CTTTCTTCTG CGCCTTAGTC ACCGCCTTCT TGGAGCCCTT TTTCGGGGCG GGAGCAGACT
     2641 TCGCTGGCTC TGGCATGGTA GATCCCCCTG GGGAGAGAGG TCGGTGATTC GGTCAACGAG
     2701 GGAGCCGACT GCCGACGTGC GCTCCGGAGG CTTGCAGAAT GCGGAACACC GCGCGGGCAG
     2761 GAACAGGGCC CACACTACCG CCCCACACCC CGCCTCCCGC ACCGCCCCTT CCCGGCCGCT
     2821 GCTCTCGGCG CGCCCTGCTG AGCAGCCGCT ATTGGCCACA GCCCATCGCG GTCGGCGCGC
     2881 TGCCATTGCT CCCTGGCGCT GTCCGTCTGC GAGGGTACTA GTGAGACGTG CGGCTTCCGT
     2941 TTGTCACGTC CGGCACGCCG CGAACCGCAA GGAACCTTCC CGACTTAGGG GCGGAGCAGG
     3001 AAGCGTCGCC GGGGGGCCCA CAAGGGTAGC GGCGAAGATC CGGGTGACGC TGCGAACGGA
     3061 CGTGAAGAAT GTGCGAGACC CAGGGTCGGC GCCGCTGCGT TTCCCGGAAC CACGCCCAGA
     3121 GCAGCCGCGT CCCTGCGCAA ACCCAGGGCT GCCTTGGAAA AGGCGCAACC CCAACCCCCT
     3181 CGAGCCGCGG CCAAAGTGGA TCTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT
     3241 TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC
     3301 TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT AAAATTGTGG ATGAATACTG
     3361 CCATTTGTCT CGAGGTCGAG AATTAAAAAC AACAAGATGA AGAGCACCAA CTCGAGTTGG
     3421 TGCTCTTCAT CTTGTTGCCG GTGTTTCGTC CTTTCCACAA GATATATAAA GCCAAGAAAT
     3481 CGAAATACTT TCAAGTTACG GTAAGCATAT GATAGTCCAT TTTAAAACAT AATTTTAAAA
     3541 CTGCAAACTA CCCAAGAAAT TATTACTTTC TACGTCACGT ATTTTGTACT AATATCTTTG
     3601 TGTTTACAGT CAAATTAATT CCAATTATCT CTCTAACAGC CTTGTATCGT ATATGCAAAT
     3661 ATGAAGGAAT CATGGGAAAT AGGCCCTCGG TGAAGGGGGC GGCCGCTCGA GGCTAGTCTC
     3721 GTGATCGATA CCGTCGAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC
     3781 CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT
     3841 GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA
     3901 CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC
     3961 AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT
     4021 GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT
     4081 AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG
     4141 ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC
     4201 AACCCCAAAT CCCCAGGAGC TGTTGATCCT TTAGGTATCT TTCCACAGCC AGGATTCTTG
     4261 CCTGGAGCTG CTTGATGCCC CAGACTGTGA GTTGCAACAG ATGCTGTTGC GCCTCAATAG
     4321 CCCTCAGCAA ATTGTTCTGC TGCTGCACTA TACCAGACAA TAATTGTCTG GCCTGTACCG
     4381 TCAGCGTCAT TGACGCTGCG CCCATAGTGC TTCCTGCTGC TCCCAAGAAC CCAAGGAACA
     4441 AAGCTCCTAT TCCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG
     4501 GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT
     4561 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGCTTGCTG TGCGGTGGTC
     4621 TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCTTGGTGTC TTTTATCTCT
     4681 ATCCTTTGAT GCACACAATA GAGGGTTGCT ACTGTATTAT ATAATGATCT AAGTTCTTCT
     4741 GATCCTGTCT GAAGGGATGG TTGTAGCTGT CCCAGTATTT GTCTACAGCC TTCTGATGTT
     4801 TCTAACAGGC CAGGATTAAC TGCGAATCGT TCTAGCTCCC TGCTTGCCCA TACTATATGT
     4861 TTTAATTTAT ATTTTTTCTT TCCCCCTGGC CTTAACCGAA TTTTTTCCCA TCGCGATCTA
     4921 ATTCTCCCCC GCTTAATACT GACGCTCTCG CACCCATCTC TCTCCTTCTA GCCTCCGCTA
     4981 GTCAAAATTT TTGGCGTACT CACCAGTCGC CGCCCCTCGC CTCTTGCCGT GCGCGCTTCA
     5041 GCAAGCCGAG TCCTGCGTCG AGAGAGCTCC TCTGGTTTCC CTTTCGCTTT CAAGTCCCTG
     5101 TTCGGGCGCC ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
     5161 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
     5221 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
     5281 ACCAGAGAGA CCCGTTTATG TATCGAGCTA GGCACTTAAA TACAATATCT CTGCAATGCG
     5341 GCAATTCAGT GGTTCGTCCA ATCCATGTCA GACCCGTCTG TTGCCTTCCT AATAAGGCAC
     5401 GATCGTACCA CCTTACTTCC ACCAATCGGC ATGCACGGTG CTTTTTCTCT CCTTGTAAGG
     5461 CATGTTGCTA ACTCATCGTT ACCATGTTGC AAGACTACAA GAGTATTGCA TAAGACTACA
     5521 TTAAGCTTGC AGCTCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA
     5581 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
     5641 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
     5701 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA
     5761 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC
     5821 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
     5881 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA
     5941 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
     6001 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     6061 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     6121 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     6181 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     6241 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     6301 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     6361 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     6421 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     6481 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     6541 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     6601 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     6661 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     6721 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     6781 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     6841 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
     6901 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     6961 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     7021 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     7081 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     7141 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     7201 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     7261 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     7321 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     7381 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     7441 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     7501 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     7561 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     7621 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     7681 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTAA
     7741 ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT
     7801 TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA
     7861 GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC
     7921 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA
     7981 TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC
     8041 CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG
     8101 AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA
     8161 CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCATTCAG GCTGCGCAAC
     8221 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA
     8281 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
     8341 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACGTCCCTA
     8401 GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG
     8461 CCTCTGCATA AAT
//