LOCUS       Exported                5693 bp ds-DNA     circular SYN 03-FEB-2025
DEFINITION  Inverted placI_Q243_Insert.
ACCESSION   .
VERSION     .
KEYWORDS    p15_FerCA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   nd
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     GenBank       5693 bp   DNA     circular
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    889..1085
                     /label=PlacI / RBS
     misc_feature    1086..1562
                     /label=FerC
     misc_feature    1613..1812
                     /label=P lacI/RBS
     misc_feature    1810..3939
                     /label=FerA
     misc_feature    join(5475..5693,1..441)
                     /label=CAT
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
      541 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
      601 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
      661 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
      721 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      781 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg
      841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggccc atcgaatggt
      901 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      961 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     1021 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     1081 cgcatatggg tgaacgtatg cgtcaggatg atcaggatgc aggcaccggt agcaccgatg
     1141 caccggctgc actgctgccg agtccgctgg ataatatgct gggttatcgt attcgtcgtg
     1201 caagcatggc aatgatgcat gatctggctc atgcactgcg tccgtttgat attagcgttg
     1261 gtgaagcaag cctgctgatt atggttggtg caaatccggg ttgtcgtcag agcgatctgg
     1321 gtcgtgcact ggaaattaaa cgtgcaaata tgacaccgct ggttggtcgt ctgaaagcac
     1381 gtggtctgat tacagatgca ccgattgatg gtcgtagcct gagcctgacc ctgaccgaag
     1441 aaggtgaagc actgcagaaa gttctgctgg cacatgccca gaccagcaat gatcgttttc
     1501 gtaaacgtct ggcactggat gaaaaagatc tgattgcagc aatggcagca ctggcacgtt
     1561 gaataaaacg aaaggctcag tcggaagact gggcctttcg ttttattcta gaccatcgaa
     1621 tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     1681 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
     1741 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
     1801 gaagcggcca tggccgttga agccggtgtt cgtcctcagg caggcgcacg tgatattaat
     1861 cgtctgctgc gtccgcgtag cattgcaatt gttggtgcaa gcgaaacacc gggtagcctg
     1921 ggtgcaagcg ttctggcaaa tctggttcgt aatgaatttc cgggtgatat tcatctggtt
     1981 aatccgaaac gtgaaaccat tagcggtcgt ccggcagttc cgagcgttga tgcactgccg
     2041 gaaggtgttg attgtgcaat tctggcaatt ccgcgtgttg cagttctgga taccatgcgt
     2101 cagctggcag cacgtaaagc cggtgcagca attatctttg cagcaggttt tgccgaaggt
     2161 ggtgaacagg gtatggcaga tcagcaagaa attggtcgta ttgcacatga agcaggtatt
     2221 gttgttgaag gtccgaattg tctgggtagc gttaattatc tggatcgtat tccgctgacc
     2281 tttatcgata ccgatattaa agcaccgagt ccgggtggtg ttggtattgt tagccagagc
     2341 ggtgcaatgg cagccgttct ggcagttatg ctggaaagcc gtaccctgga tctgacctat
     2401 agcgttagca ccggtaatga agccggtagc ggtgttgaag attatgtgga atttatgatc
     2461 gccgatgaaa aaacccgtat tattgccatg attgtggaac agtttcgtga tccggctcgt
     2521 tttctggcac tgatggataa agcaaatgca gcaggtaaac tggttgttct gctgcatccg
     2581 ggtaaaagca gcgcagcacg tgaaagcgca gcaacccata caggcgcaat ggcaggcgat
     2641 tataaactga tgcgtgcaaa agttgaacgt gccggtgttg ttgttgcaga aaccctggaa
     2701 gaactgggtg atattaccga aattgcagca cgttgtccgg cactgccgag cggtggcaca
     2761 gcagttctgg gtgaaagcgg tgcactgaaa gcactgacac tggatctggc agaagaactg
     2821 gaactggcac tgccgaccct ggatgatggt aatgcaccgg cactgcgtgc agccctgccg
     2881 gaatttgttc cggttagcaa tccgctggat ctgacagcac agggtctggt tgatccggat
     2941 atgtattatc gtaccctggc agcactgttt gaagatgatc gtgttggcac catttttgcc
     3001 ggtattattc agaccaatcc ggcaaccatt ggtattaaac tgccaccgtt tctgaaagca
     3061 gttcgtgaac tgaaagcaac caaaccggtt atttttggtg gtgttgatga aggtgcagat
     3121 gttccggcag attggattga acagctgcgt gcagaaggta ttccgtattt tccgaccacc
     3181 gaacgtgcac tgcgtgccat tcgtcgtctg agcgcagcgg gtgcacgtga tgcaagccgt
     3241 accgatgcag caccggcaag cgttccggca ctggcaagcg aaaaaggtgt tgttccggaa
     3301 tataaagcaa aagcactgct ggcaccgctg ggtattagct ttccgaaagg tcagtttgca
     3361 gcaaccgttg aagatgcaat tgcagcagcc gaagcaattg gtggtccggt tgttatgaaa
     3421 gcacaggcag cagcactgag ccataaaagt gatgccggtg gtgttgcact gaatctggtt
     3481 ggtgcggaag caattcgtgc agcatgggat aaaatgtttg cagatgtgaa acgttatgac
     3541 gccagcatta ttctggatgg tgttctgatc gaagcaatgg gtgcccgtgg tctggaactg
     3601 attgttggtg ccaaaaatga tccgcagtgg ggtccggtta ttctggcagg ttttggtggc
     3661 gttaccgcag aaattctgca ggatgttcgt ctgctgtcac cggatatgac caaagaagca
     3721 attgttgcag aactgggtaa actgaaacag gcagcactgc tgcatggtta tcgtggtagt
     3781 ccggcactgg atgttggtgc agttgccgaa ctgattggtc aggtgggtcg cctgctgcgt
     3841 ggcgaaccgc gtattcaaga actggatctg aatccggttg ttgtttatcc ggaaggtcag
     3901 ggtgcaattg ccctggatgc actgatgctg gttgattgaa taaaacgaaa ggctcagtcg
     3961 gaagactggg cctttcgttt tatggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4021 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4081 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt
     4141 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta acatgagaat
     4201 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
     4261 gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
     4321 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
     4381 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     4441 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
     4501 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
     4561 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     4621 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     4681 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     4741 cgccagggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     4801 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
     4861 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
     4921 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     4981 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc
     5041 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac
     5101 actccgctag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
     5161 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
     5221 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     5281 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     5341 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     5401 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     5461 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     5521 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     5581 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     5641 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttc
//
